Mass Production of the Nuclear Polyhedrosis Virus (NPV) of Helicoverpa armigera and Spodoptera litura

2021 ◽  
pp. 185-194
Author(s):  
R. Rajeshwari
Keyword(s):  
Helicoverpa Armigera ◽  
Mass Production ◽  
Spodoptera Litura ◽  
Nuclear Polyhedrosis Virus ◽  
Helicoverpa Armígera ◽  
Polyhedrosis Virus ◽  
Nuclear Polyhedrosis

scholarly journals Biological Test of Formulation of Subculture Helicovepa armigera Nuclear Polihedrosis Virus (HaNPV) on Mortality of Spodoptera litura Larvae infested to cabbage (Brassica oleracea Var. capitata Linn.) Plantation

2015 ◽  
Vol 2 (1) ◽  
pp. 646
Author(s):  
Desy Efriyani Anggraeny Nasution ◽  
Mia Miranti ◽  
Melanie .
Keyword(s):  
Brassica Oleracea ◽  
Helicoverpa Armigera ◽  
Spodoptera Litura ◽  
Nuclear Polyhedrosis Virus ◽  
Insect Pests ◽  
Block Design ◽  
Economic Value ◽  
Helicoverpa Armígera ◽  
Polyhedrosis Virus ◽  
Nuclear Polyhedrosis

<p>Helicoverpa armigera Nuclear Polyhedrosis Virus (HaNPV) is one of the biological agents that could be used for the development of organic farming in Indonesia. One of the organic agricultural food products which have high economic value is cabbage, HaNPV effectif to control the population of several species of insect pests, can be used instead of synthetic insecticides in agricultural development efforts of organic cabbage. HaNPV formulation can be used as a way to maintain the virulence properties of the virus and facilitate the storage and application. The preparation of subculture HaNPV formulations was in liquid, powders, cornstarch and talk carrier material. The design experimental was used randomized block design consists single factor (type HaNPV formulation consisting of powder formulations, liquid formulation, formulation in cornstarch, and talc formulation including water formulation without virus as a control), with eight replications. The results showed that all of the formulations of subculture HaNPV causing larvae mortality at range 97.4% -100%. The highest mortality of larvae occurred in cabbage plants are protected by HaNPV carrier liquid and talk preparations by 100% each. <br /><strong></strong></p><p><strong>Keywords</strong>: Formulations Helicoverpa armigera Nuclear Polyhedrosis Virus (HaNPV), subculture, Spodoptera litura, Brassica oleracea Var. capitata Linn.</p>

scholarly journals THE POTENTIAL OF SUBCULTURE Helicoverpa armigera NUCLEAR POLYHEDROSIS VIRUS (HaNPV) TO BE UTILIZED AS AN ALTERNATE SYNTHETIC INSECTICIDES TO CONTROL INSECTS PESTS IN CABBAGES PLANTATION (Brassica oleracea var. capitata L)

2015 ◽  
Vol 2 (1) ◽  
pp. 405
Author(s):  
Mia Miranti ◽  
Wawan Hermawan ◽  
Melanie . ◽  
Rahman Perdana Hadi ◽  
Dicky Budi Sugiarto ◽  
...  
Keyword(s):  
Helicoverpa Armigera ◽  
Spodoptera Litura ◽  
Nuclear Polyhedrosis Virus ◽  
Viral Agent ◽  
Alternate Host ◽  
Randomized Design ◽  
Helicoverpa Armígera ◽  
Polyhedrosis Virus ◽  
Nuclear Polyhedrosis ◽  
Entomopathogenic Viruses

<p>Subculture Helicoverpa armigera Nuclear Polyhedrosis Virus (HaNPV) is an entomopathogenic viruses isolated from cadaver of Helicoverpa armigera larvae, and was succesfully produced in Spodoptera litura larvae as an alternate host. These viral agent have been applied to control the population of three species insects pests Spodoptera litura, Crocidolomia pavonana and Plutella xyllostella which were infested in Cabbages Plantation. The concentration of virus of 4 x 107 Polyhedral/ml was sprayed in cabbage leaves 24 hours before infested of larval and every four days after. The research used randomized design which consisted of factor (three species of second instar larvae, S. litura, C. pavonana, P. xyllostella) and eight replications. The result showed that the three species of larval were sensitive against subculture HaNPV infection. The mortality of each larval species (S. litura, C. pavonana and P. xyllostella) were 100%, 97.5% and 98.7%. Subculture HaNPV can be utilized to control the population of three spesies of insects pests in Cabbage Plantation. </p><p>Keywords : Subculture HaNPV, Polyhedral</p>

scholarly journals DAYA PATOGENESITAS Helicoverpa armigera Nuclear Polyhedrosis Virus HaNPV SETELAH TERKENA RADIASI SINAR ULTRAVIOLET

2000 ◽  
Vol 6 (1) ◽  
pp. 65-70
Author(s):  
Mahanani Tri Asri ◽  
Isnawati Isnawati
Keyword(s):  
Biological Control ◽  
Helicoverpa Armigera ◽  
Nuclear Polyhedrosis Virus ◽  
Uv Light ◽  
Ultra Violet ◽  
Ultra Violet Light ◽  
Violet Light ◽  
Helicoverpa Armígera ◽  
Polyhedrosis Virus ◽  
Nuclear Polyhedrosis

This research is about pathogen capacity of the Helicoverpa armigera nuclear Polyhedrosis Virus (HaNPV) after irradiation by ultra violet (UV) light. The HaNPV is the one kind of the virus which attack the insect Helicoverpa armigera-attacker the some species of the crop life tobacco, cotton, potato, tomato. Because of that the HaNPV can be used as the biological control of the pest. Some researcher stated that the pathogen capacity of the HaNPV is decrease if the virus was expose in the ultra violet (UV) light. Because of the fact the virus in not useful as the biological control of the pest in the land corp. This research to test the statement. Laboratory experimental was done to tested the pathogen capacity of the HaNPV after irradiation treatment by the ultra violet light. The intensity of the UV light in this experiment were 28.7 lux, 97.3 lux, and 127.4 lux with the lighting period were 0, 1, 3, 6, 9, 12, and 15. The result of the research stated that the pathogen capacity of the HaNPV was not influenced by ultra violet light. So the virus is still useful as the biological control of the pest in the land crop.

scholarly journals EFISIENSI PENGENDALIAN PENGGEREK BUAH KAPAS Helicoverpa armigera HÜBNER DENGAN SERBUK BIJI MIMBA DAN NUCLEAR POLYHEDROSIS VIRUS

2020 ◽  
Vol 12 (2) ◽  
pp. 45
Author(s):  
I G.A.A. INDRAYANI ◽  
DWI WINARNO ◽  
TEGER BASUKI BASUKI
Keyword(s):  
Helicoverpa Armigera ◽  
Nuclear Polyhedrosis Virus ◽  
Rate Of Return ◽  
Recommended Dose ◽  
Neem Seed ◽  
Marginal Rate ◽  
Helicoverpa Armígera ◽  
Marginal Rate Of Return ◽  
Polyhedrosis Virus ◽  
Nuclear Polyhedrosis

ABSTRAK<br />Pengendalian hama non-kimiawi semakin meningkat sehingga<br />mengurangi penggunaan insektisida kimia. Alternatif pengendalian hama<br />menggunakan pestisida botani dan agensia mikrobia cukup efektif<br />mengendalikan penggerek buah kapas H. armigera. Penelitian efisiensi<br />pengendalian penggerek buah kapas H. armigera dengan SBM dan NPV<br />dilakukan di Kebun Percobaan Balai Penelitian Tanaman Tembakau dan<br />Serat di Asembagus, Situbondo, Jawa Timur mulai Januari hingga<br />Desember 2003. Tujuannya adalah untuk mengetahui tingkat efisiensi<br />pengendalian penggerek buah kapas H. armigera terutama dengan<br />kombinasi SBM dan NPV. Perlakuan yang digunakan adalah: (1)<br />SBM(LC 25 )+NPV(LC 50 ), (2) SBM(LC 50 )+NPV(LC 50 ), (3) SBM (dosis<br />rekomendasi), (4) NPV (dosis rekomendasi), (5) betasiflutrin (dosis<br />rekomendasi), dan (6) kontrol (tanpa perlakuan). Setiap perlakuan disusun<br />dalam rancangan acak kelompok (RAK) dengan tiga ulangan. Parameter<br />yang diamati meliputi populasi ulat H. armigera dan kompleks<br />predatornya, frekuensi penyemprotan masing-masing perlakuan, kerusakan<br />kuncup bunga dan buah kapas, biaya pengendalian hama, pendapatan,<br />marginal rate of return (MRR), dan hasil kapas serta kacang hijau. Hasil<br />penelitian menunjukkan bahwa pengendalian H. armigera dengan<br />kombinasi perlakuan SBM(LC 50 )+NPV(LC 50 ) lebih efisien menurunkan<br />biaya pengendalian hama hingga 63,4% dan meningkatkan pendapatan<br />sebesar 32,7% dibanding insektisida kimia betasiflutrin, dengan nilai MRR<br />4,66 dan 4,28 masing-masing atas kontrol dan insektisida kimia.<br />Kata kunci: Kapas, Gossypium hirsutum, hama, penggerek buah,<br />Helicoverpa armigera, SBM, NPV, pengendalian hama,<br />marginal rate of return, Jawa Timur<br />ABSTRACT<br />Efficiency in cotton bollworm Helicoverpa armigera Hübner control<br />using neem seed powder and nuclear polyhedrosis virus<br />Insect pest biological control potentially decreases the use of<br />chemical insecticides. The alternative control method chosen was<br />combination of botanical and microbial agents that showed higher<br />effectiveness against H. armigera. This control method might also<br />potential to minimize the use of chemical pesticide on cotton. Study on<br />efficiency of cotton bollworm Helicoverpa armigera Hübner control using<br />neem seed powder (NSP) and nuclear polyhedrosis virus was conducted at<br />Asembagus Experimental Station of Indonesian Tobacco and Fiber Crops<br />Research Institute, Situbondo, East Java, from January to December 2003.<br />The objective of this study was to find out the efficiency level of cotton<br />bollworm control using combination of neem seed powder (NSP) and<br />nuclear polyhedrosis virus (NPV). The treatments were : (1) NSP(LC 25 ) +<br />NPV(LC 50 ), (2) NSP(LC 50 ) + NPV(LC 50 ), (3) NSP (recommended dose),<br />(4) NPV (recommended dose), (5) betacyfluthrin (recommended dose),<br />and (6) control (untreated). The treatments were arranged in a randomized<br />block design with three replications. Parameters observed were population<br />of H. armigera larvae and its complex predators, frequency of spraying,<br />square and boll damage, cost of control, net income, marginal rate of<br />return, and yield of seed cotton and mungbean. The research results<br />showed that the combination of NSP(LC 50 )+NPV(LC 50 ) effectively<br />reduced the total cost of insect control by 63.4% and increased the increase<br />32.7% compared to betacyfluthrin. The combination also showed the<br />highest marginal rate of return of 4.66 and 4.28 based on control and<br />betacyfluthrin, respectively.<br />Key words: Cotton, Gossypium hirsutum, insect, cotton bollworm,<br />Helicoverpa armigera, NSP, NPV, insect control, marginal<br />rate of return, East Java

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