In Situ Hybridization in Light Microscopy

2000 ◽  
Author(s):  
Gerard Morel ◽  
Annie Cavalier
Keyword(s):  
In Situ Hybridization ◽  
Light Microscopy

Methanosaeta fibers in anaerobic migrating blanket reactors

2000 ◽  
Vol 41 (4-5) ◽  
pp. 35-39 ◽  
Author(s):  
L.T. Angenent ◽  
D. Zheng ◽  
S. Sung ◽  
L. Raskin
Keyword(s):  
Electron Microscopy ◽  
Fatty Acids ◽  
Scanning Electron Microscopy ◽  
In Situ Hybridization ◽  
Light Microscopy ◽  
Volatile Fatty Acids ◽  
Dense Structure ◽  
Scanning Electron ◽  
Granular Blanket

An anaerobic migrating blanket reactor (AMBR) was seeded with flocculent biomass from a digester and fed a substrate consisting of volatile fatty acids and sucrose to study granulation. After three months of operation, a mature granular blanket developed in the reactor. Moreover, fibers of approximately 1 cm long had become prevalent in the AMBR. Scanning electron microscopy (SEM) and light microscopy revealed a very dense structure consisting of bundles of filaments resembling Methanosaeta cells. Further studies with fluorescence in-situ hybridization (FISH), showed that Methanosaeta concilii was the predominant microorganism in these fibers.

Preparing plant chromosomes for scanning electron microscopy

Genome ◽  
1990 ◽  
Vol 33 (3) ◽  
pp. 333-339 ◽  
Author(s):  
John E. Dillé ◽  
Douglas C. Bittel ◽  
Kathleen Ross ◽  
J. Perry Gustafson
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
In Situ Hybridization ◽  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
Light Microscopy ◽  
Plant Chromosomes ◽  
Cellular Debris ◽  
Scanning Electron

The scanning electron microscope may be useful in the analysis of plant chromosomes treated with in situ hybridization, especially when the probes and (or) chromosomes are near or beyond the resolution of the light microscope. Usual methods of plant chromosome preparation are unsuitable for scanning electron microscope observation as a result of cellular debris, which also interferes with probe hybridization. A method is described whereby protoplasts are obtained from fixed root tips by enzymatic digestion and applied to slides in a manner that produces little or no cellular debris overlying the chromosomes. The slides were examined by scanning electron microscopy and light microscopy after C-banding and in situ hybridization with a rye nucleolus organizer region spacer probe. This technique, which allows for scanning electron microscope visualization of bands and probes not easily identified with light microscopy, should prove useful in the physical mapping of low copy number or unique DNA sequences.Key words: protoplasts, rice, wheat, rye, physical maps, in situ hybridization.

HIV detection by in-situ hybridization based on confocal reflected light microscopy

1991 ◽  
Author(s):  
Louis C. Smith ◽  
Zeljko Jericevic ◽  
Roland Cuellar ◽  
Stephen W. Paddock ◽  
Dorothy E. Lewis
Keyword(s):  
In Situ Hybridization ◽  
Light Microscopy ◽  
Reflected Light

Immunocytochemistry. A Review of Methodology for Electron Microscopic Applicaiton

1974 ◽  
Vol 32 ◽  
pp. 328-329
Author(s):  
Joseph E. Mazurkiewicz
Keyword(s):  
Electron Microscopy ◽  
Light Microscopy ◽  
Ultrastructural Level ◽  
Electron Microscopic ◽  
Biological Interest ◽  
Investigative Approach ◽  
Immunologic Activity ◽  
Dense Label

Immunocytochemistry is a powerful investigative approach in which one of the most exacting examples of specificity, that of the reaction of an antibody with its antigen, isused to localize tissue and cell specific molecules in situ. Following the introduction of fluorescent labeled antibodies in T950, a large number of molecules of biological interest had been studied with light microscopy, especially antigens involved in the pathogenesis of some diseases. However, with advances in electron microscopy, newer methods were needed which could reveal these reactions at the ultrastructural level. An electron dense label that could be coupled to an antibody without the loss of immunologic activity was desired.

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