Localization of pituitary adenylate cyclase-activating polypeptide and its messenger ribonucleic acid in the rat testis by light and electron microscopic immunocytochemistry and in situ hybridization.

Endocrinology ◽  
1994 ◽  
Vol 135 (3) ◽  
pp. 818-825 ◽  
Author(s):  
S Shioda ◽  
G Legradi ◽  
W C Leung ◽  
S Nakajo ◽  
K Nakaya ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Adenylate Cyclase ◽  
Ribonucleic Acid ◽  
Electron Microscopic ◽  
Electron Microscopic Immunocytochemistry ◽  
Messenger Ribonucleic Acid ◽  
Rat Testis ◽  
Pituitary Adenylate Cyclase

scholarly journals Prolactin Receptor Messenger Ribonucleic Acid in Normal and Neoplastic Human Pituitary Tissues1

1997 ◽  
Vol 82 (3) ◽  
pp. 963-968
Author(s):  
Long Jin ◽  
Xiang Qian ◽  
Elzbieta Kulig ◽  
Bernard W. Scheithauer ◽  
Rocio Calle-Rodrigue ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Reverse Transcription ◽  
Ribonucleic Acid ◽  
Prolactin Receptor ◽  
Pituitary Surgery ◽  
Specific Cell ◽  
Human Pituitary ◽  
Messenger Ribonucleic Acid ◽  
Reverse Transcription Pcr

Abstract We examined the specific cell types in normal human pituitaries that expressed PRL receptor (PRL-R) messenger ribonucleic acid (mRNA) by combined in situ hybridization and immunohistochemistry. The distribution of PRL-R mRNA in 28 pituitary adenomas was examined by in situ hybridization and reverse transcription-PCR in 12 cases of adenomas. In another set of experiments, 34 PRL adenomas from men, women, and bromocriptine-treated patients were analyzed for PRL-R by in situ hybridization. In the normal pituitary, PRL- and LH-producing cells had significantly more mean grain counts per cell and higher percentages of cells positive for PRL-R than GH and TSH cells. PRL-R mRNA was present in all groups of adenomas by in situ hybridization and reverse transcription-PCR. PRL adenomas had a significantly higher density of labeling compared to other adenoma types. Although there was no difference in the levels of PRL-R mRNA in PRL adenomas from men and premenopausal and postmenopausal women, patients treated with bromocriptine before pituitary surgery had significantly lower levels of PRL-R compared to all other groups. These results indicate that in the normal pituitary, PRL and LH cells have the highest level of PRL-R mRNA, whereas PRL adenomas have significantly higher levels of PRL-R mRNA than other types of adenomas, and bromocriptine treatment decreases the levels of PRL-R mRNA in PRL adenomas.

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