In Vitro Comparison of Recombinant and Plasma-Derived von Willebrand Factor Concentrate for Treatment of Acquired von Willebrand Syndrome in Adult Extracorporeal Membrane Oxygenation Patients

2021 ◽  
Vol Publish Ahead of Print ◽  
Author(s):  
Michael Mazzeffi ◽  
Reney Henderson ◽  
Eric Krause ◽  
Joseph Rabin ◽  
Ronson Madathil ◽  
...  
Keyword(s):  
Extracorporeal Membrane Oxygenation ◽  
Von Willebrand Factor ◽  
Acquired Von Willebrand Syndrome ◽  
Membrane Oxygenation ◽  
Factor Concentrate ◽  
Von Willebrand ◽  
Willebrand Factor ◽  
In Vitro Comparison

In vitro Evaluation of a Very-High-Purity, Solvent/Detergent-Treated, Von Willebrand Factor Concentrate

Vox Sanguinis ◽  
1991 ◽  
Vol 61 (1) ◽  
pp. 1-7
Author(s):  
Claudine Mazurier ◽  
Sylvie Jorieux ◽  
Christophe de Romeuf ◽  
Bruno Samor ◽  
Maurice Goudemand
Keyword(s):  
Von Willebrand Factor ◽  
High Purity ◽  
In Vitro Evaluation ◽  
Factor Concentrate ◽  
Von Willebrand ◽  
Willebrand Factor ◽  
Very High

In vitro study of a triple-secured von Willebrand factor concentrate

Vox Sanguinis ◽  
2004 ◽  
Vol 86 (2) ◽  
pp. 100-104 ◽  
Author(s):  
C. Mazurier ◽  
M. Poulle ◽  
B. Samor ◽  
L. Hilbert ◽  
S. Chtourou
Keyword(s):  
Von Willebrand Factor ◽  
In Vitro Study ◽  
Vitro Study ◽  
Factor Concentrate ◽  
Von Willebrand ◽  
Willebrand Factor

von Willebrand Factor Multimers in Pediatric Extracorporeal Membrane Oxygenation Support

ASAIO Journal ◽  
2014 ◽  
Vol 60 (4) ◽  
pp. 419-423 ◽  
Author(s):  
Sanjiv Pasala ◽  
Richard T. Fiser ◽  
Kimo C. Stine ◽  
Christopher J. Swearingen ◽  
Parthak Prodhan
Keyword(s):  
Extracorporeal Membrane Oxygenation ◽  
Von Willebrand Factor ◽  
Membrane Oxygenation ◽  
Von Willebrand ◽  
Willebrand Factor

In vitro Evaluation of a Very-High-Purity, Solvent/Detergent-Treated, Von Willebrand Factor Concentrate

Vox Sanguinis ◽  
1991 ◽  
Vol 61 (1) ◽  
pp. 1-7 ◽  
Author(s):  
Claudine Mazurier ◽  
Sylvie Jorieux ◽  
Christophe Romeuf ◽  
Bruno Samor ◽  
Maurice Goudemand
Keyword(s):  
Von Willebrand Factor ◽  
High Purity ◽  
In Vitro Evaluation ◽  
Factor Concentrate ◽  
Von Willebrand ◽  
Willebrand Factor ◽  
Very High

Abnormal Structure of von Willebrand Factor in Myeloproliferative Syndrome Is Associated to Either Thrombotic or Bleeding Diathesis

1987 ◽  
Vol 58 (02) ◽  
pp. 753-757 ◽  
Author(s):  
M F López-Fernández ◽  
C López-Berges ◽  
R Martín ◽  
A Pardo ◽  
F J Ramos ◽  
...  
Keyword(s):  
Von Willebrand Factor ◽  
Proteinase Inhibitors ◽  
Relative Proportion ◽  
Variable Degree ◽  
Bleeding Diathesis ◽  
Myeloproliferative Syndrome ◽  
Von Willebrand ◽  
Willebrand Factor

SummaryThe multimeric and subunit patterns of plasma von Willebrand factor (vWF) were analyzed in eight patients with myeloproliferative syndrome (MS) in order to investigate the possible existence of heterogeneity in the “in vivo” proteolytic cleavage of the protein, previously observed in this entity. Six patients lacked large vWF multimers, five of them having normal bleeding times (BT) and clinically documented episodes of thrombotic origin, whereas one patient had long BT and bleeding symptoms. Seven patients showed a relative increase in the 176 kDa subunit fragment while the 189 kDa polypeptide was increased in only one. In addition, another patient (and prior to any therapy) showed the presence of a new fragment of approximately 95 kDa which disappeared after Busulfan therapy. The collection of blood from these patients with proteinase inhibitors did not correct the abnormalities.The infusion of DDAVP to two patients with abnormal vWF was accompanied by: the appearance of larger vWF multimers which disappeared rapidly from plasma; an increase in the relative proportion of the satellite bands of each multimer and a further increase of the 176 kDa fragment. These data point to some heterogeneity in the vWF abnormality present in MS which may be related in part to a variable degree of proteolysis of vWF occurring “in vivo” rather than “in vitro”, and which may be associated to either a thrombotic or a bleeding diathesis. They also suggest that despite the presence of abnormal, already proteolyzed vWF, DDAVP-enhanced proteolysis occurs in MS to a similar extent to what is described in normal individuals.

Adverse Influence of Cigarette Smoking on the Endothelium

1993 ◽  
Vol 70 (04) ◽  
pp. 707-711 ◽  
Author(s):  
Andrew D Blann ◽  
Charles N McCollum
Keyword(s):  
Endothelial Cells ◽  
Von Willebrand Factor ◽  
Tobacco Smoke ◽  
Lipid Peroxides ◽  
Short Exposure ◽  
Acute Effects ◽  
Adverse Influence ◽  
Von Willebrand ◽  
Willebrand Factor

SummaryThe effect of smoking on the blood vessel intima was examined by comparing indices of endothelial activity in serum from smokers with that from non-smokers. Serum from smokers contained higher levels of von Willebrand factor (p <0.01), the smoking markers cotinine (p <0.02) and thiocyanate (p <0.01), and was more cytotoxic to endothelial cells in vitro (p <0.02) than serum from non-smokers. The acute effects of smoking two unfiltered medium tar cigarettes was to briefly increase von Willebrand factor (p <0.001) and cytotoxicity of serum to endothelial cells in vitro (p <0.005), but lipid peroxides or thiocyanate were not increased by this short exposure to tobacco smoke. Although there were correlations between von Willebrand factor and smokers consumption of cigarettes (r = 0.28, p <0.02), number of years smoking (r = 0.41, p <0.001) and cotinine (r = 0.45, p <0.01), the tissue culture of endothelial cells with physiological levels of thiocyanate or nicotine suggested that these two smoking markers were not cytotoxic. They are therefore unlikely to be directly responsible for increased von Willebrand factor in the serum of smokers. We suggest that smoking exerts a deleterious influence on the endothelium and that the mechanism is complex.

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