scholarly journals The influence of food restriction versus ad libitum feeding of chow and purified diets on variation in body weight, growth and physiology of female Wistar rats

2012 ◽  
Vol 46 (2) ◽  
pp. 101-107 ◽  
Author(s):  
M Moraal ◽  
P P A M Leenaars ◽  
H Arnts ◽  
K Smeets ◽  
B S Savenije ◽  
...  
Keyword(s):  
Body Weight ◽  
Food Restriction ◽  
Wistar Rats ◽  
Statistical Significance ◽  
Blood Parameters ◽  
Calorie Intake ◽  
Restricted Feeding ◽  
Organ Weights ◽  
Female Wistar Rats ◽  
Ad Libitum

Ad libitum (AL) supply of standard chow is the feeding method most often used for rodents in animal experiments. However, AL feeding is known to result in a shorter lifespan and decreased health as compared with restricted feeding. Restricted feeding and thus limiting calorie intake prevents many health problems, increases lifespan and can also increase group uniformity. All this leads to a reduced number of animals needed. So-called standard chows are known to be prone to variation in composition. Synthetic foods have a more standard composition, contributing to group uniformity which, like diet reduction, may decrease the number of animals necessary to obtain statistical significance. In this study, we compared the effects of AL versus restricted feeding (25% reduction in food intake) on standard chow versus synthetic food of three different suppliers on body weight (BW), growth, several blood parameters and organ weights in growing female Wistar rats over a period of 61 days. Diet restriction led to a decreased growth and significantly reduced variation in BW and growth as compared with AL feeding. AL feeding on synthetic diets caused a significantly higher BW gain than on chow diets. Due to experimental design, this same effect occurred on food restriction. Blood parameters and organ weights were affected neither by diet type nor by amount. Incidentally, variations were significantly reduced on food restriction versus AL, and on synthetic diets versus chow diets. This study demonstrates that food restriction versus AL feeding leads to a significantly reduced variation in BW and growth, thereby indicating the potential for reduction when applying this feeding schedule.

scholarly journals Effect of 4-h time restricted feeding on body weight, leptin concentration and lipid profile in healthy non-obese male Wistar rats

2021 ◽  
Author(s):  
Michael A. Olamoyegun ◽  
Folasade O. Ajao ◽  
Marcus O. Iyedupe
Keyword(s):  
Body Weight ◽  
Blood Glucose ◽  
Lipid Profile ◽  
Wistar Rats ◽  
Metabolic Diseases ◽  
Restricted Feeding ◽  
Mean Values ◽  
Male Wistar Rats ◽  
Ad Libitum ◽  
Obese Male

Abstract Background: Obesity greatly increases the risk of metabolic diseases and preventive approaches for obesity are often inadequate to effectively prevent and manage the diseases. Altering feeding time strategy intervention decreases caloric intake without calorie counting and may be an effective therapy. Therefore, this study investigates the effect of 4-h time restricted feeding on body weight, leptin concentration and lipid profile in healthy non-obese male Wistar rats. Methods: Rats placed on time-restricted feeding (TRF) regimen had freely access to food for 4 hour per day at designated periods. Twenty four rats divided into four groups (n=6) were used. Group I animals were placed on a 4 hour per day TRF between 8am-12noon. Group II rats were also placed on a 4 hour per day TRF between 12noon-4pm. Group III rats also placed on a 4 hour per day TRF between 8pm-12 midnight while Group IV rats had access food and water ad libitum. This diet strategy resembles taking only breakfast, lunch or dinner once a day. The study lasted for a period of 4 weeks with daily food intake and weekly body weight determined throughout the period. At the end of the experimental period, blood glucose, lipid profile and leptin concentration were assessed. SPSS 21.0 package was used for data analysis, one-way analysis of variance (ANOVA) was used to compare the mean values of variables among the groups and bonferroni’s posthoc test was used for significance of pair wise comparisons of mean values among the groups. Significance was set at p < 0.05.Results: In this study, the body weights and leptin concentrations of 8pm – 12am and ad libitum groups significantly increased compared with the 8am - 12noon and 12noon -4pm groups. Dyslipidemia was observed in the ad libitum group when compared with the 8am - 12noon and 12noon - 4pm groups. Conclusion: From this study, 4-hr time restricted feeding has beneficial effects on body weight, blood glucose, lipid profile and leptin concentration. This feeding restriction patterns may be helpful in obesity management and in preventing metabolic diseases development in non obese.

A COMPARISON OF THE UTILIZATION OF RAPESEED OIL AND CORN OIL BY THE RAT

1959 ◽  
Vol 37 (5) ◽  
pp. 613-621 ◽  
Author(s):  
Joyce L. Beare ◽  
T. K. Murray ◽  
H. C. Grice ◽  
J. A. Campbell
Keyword(s):  
Body Weight ◽  
Vitamin A ◽  
Rapeseed Oil ◽  
Wistar Rats ◽  
Corn Oil ◽  
Weekly Interval ◽  
Organ Weights ◽  
Weight Gains ◽  
Ad Libitum ◽  
Liver Storage

The effects of Golden rapeseed oil and corn oil on weekly weight gains, food consumptions, liver storage of vitamin A, plasma and adrenal cholesterol concentrations, organ weights, and testes histology were determined in Wistar rats for 5 weeks after weaning. At each weekly interval, animals fed rapeseed oil showed lesser weight gains and food consumptions, but, when body weight gains were adjusted for food consumptions by covariance analyses, differences largely disappeared. Liver storage of vitamin A was similar with both oils. Testes of animals fed rapeseed oil exhibited no alteration in cellular characteristics, but a reduction in tubular size. When fed to older rats, rapeseed oil again produced smaller weight gains which were accounted for at 4 weeks' time by a depressed appetite.Similar weight gains were obtained with corn oil and rapeseed oil when the oils were paired fed and when adjustments for food consumption were made by covariance analyses of weight gains of animals receiving the oils ad libitum. With corn oil supplied on a restricted basis the testicular tubules were smaller than those obtained with unrestricted feeding of corn oil. The absorption of corn oil and rapeseed oil fed ad libitum was 95 and 92% respectively. It was concluded that the two oils were not very differently utilized.

Serum leptin is not correlated with body fat in severe food restriction

2012 ◽  
Vol 37 (6) ◽  
pp. 1063-1071 ◽  
Author(s):  
Sarah E. Gairdner ◽  
Catherine E. Amara
Keyword(s):  
Body Composition ◽  
Body Mass ◽  
Body Fat ◽  
Fat Mass ◽  
Food Restriction ◽  
Wistar Rats ◽  
Serum Leptin ◽  
Wide Range ◽  
Female Wistar Rats ◽  
Ad Libitum

Leptin is an adipose-derived hormone with established roles in energy balance that can impact the response to refeeding after malnutrition. Although the amount of circulating leptin has traditionally been associated with the amount of adipose tissue, controversy exists as to whether this relationship is constant in both humans and animals and over a wide range of body composition. Our objective was to evaluate whether the leptin – body fat ratio is constant in the range of healthy to low body mass in female Wistar rats. Eight ad libitum fed (C) and eight food-restricted (FR) rats were compared over a period of four weeks. FR rats attained the target 75% of baseline body mass after the first two weeks, which was maintained for the remaining two weeks. Serum leptin and IGF-1 (ELISA) and body composition (DXA) were measured at baseline (t0) and once weekly for the remainder of the study (t1–t4). The leptin – body fat ratio was reduced during the two-week period of weight loss (t0 = 0.036 ± 0.016 (ng·mL–1)·g–1 vs. t1 = 0.010 ± 0.004 (ng·mL–1)·g–1 and t2 = 0.015 ± 0.007 (ng·mL–1)·g–1, p < 0.05). Leptin concentration plateaued at its nadir (~0.24 (ng·mL–1)·g–1) at fat mass < 22 g. IGF-1 was correlated with lean mass (r = 0.45, p < 0.05) and fat mass (r = 0.58, p < 0.05), regardless of body mass. We concluded that the leptin – body fat ratio was reduced early in food restriction and the correlation between these two variables was absent at low body fat.

A COMPARISON OF THE UTILIZATION OF RAPESEED OIL AND CORN OIL BY THE RAT

1959 ◽  
Vol 37 (1) ◽  
pp. 613-621 ◽  
Author(s):  
Joyce L. Beare ◽  
T. K. Murray ◽  
H. C. Grice ◽  
J. A. Campbell
Keyword(s):  
Body Weight ◽  
Vitamin A ◽  
Rapeseed Oil ◽  
Wistar Rats ◽  
Corn Oil ◽  
Weekly Interval ◽  
Organ Weights ◽  
Weight Gains ◽  
Ad Libitum ◽  
Liver Storage

The effects of Golden rapeseed oil and corn oil on weekly weight gains, food consumptions, liver storage of vitamin A, plasma and adrenal cholesterol concentrations, organ weights, and testes histology were determined in Wistar rats for 5 weeks after weaning. At each weekly interval, animals fed rapeseed oil showed lesser weight gains and food consumptions, but, when body weight gains were adjusted for food consumptions by covariance analyses, differences largely disappeared. Liver storage of vitamin A was similar with both oils. Testes of animals fed rapeseed oil exhibited no alteration in cellular characteristics, but a reduction in tubular size. When fed to older rats, rapeseed oil again produced smaller weight gains which were accounted for at 4 weeks' time by a depressed appetite.Similar weight gains were obtained with corn oil and rapeseed oil when the oils were paired fed and when adjustments for food consumption were made by covariance analyses of weight gains of animals receiving the oils ad libitum. With corn oil supplied on a restricted basis the testicular tubules were smaller than those obtained with unrestricted feeding of corn oil. The absorption of corn oil and rapeseed oil fed ad libitum was 95 and 92% respectively. It was concluded that the two oils were not very differently utilized.

Olfactory bulbectomy counteracts inhibitory effect of food restriction on reproductive function

1994 ◽  
Vol 266 (6) ◽  
pp. R1891-R1895
Author(s):  
D. R. Pieper ◽  
C. A. Lobocki ◽  
K. H. Karo
Keyword(s):  
Body Weight ◽  
Food Restriction ◽  
Reproductive Function ◽  
Inhibitory Effect ◽  
Olfactory Bulbs ◽  
Gonadotropin Secretion ◽  
Golden Hamsters ◽  
Testicular Regression ◽  
Effect Of Food ◽  
Ad Libitum

Previous studies have shown that bilateral removal of the olfactory bulbs (BX) results in a large increase in gonadotropin secretion in golden hamsters. The principal question addressed by the present study was whether BX would offset the inhibitory effect of food restriction on reproductive function. BX or sham (SH) BX male golden hamsters were fed ad libitum or were restricted to only enough food to maintain them at 70% of the body weight of control groups fed ad libitum. The SH-70% group underwent marked testicular regression after approximately 6-8 wk, but the testes size of the BX-70% hamsters decreased only in proportion to the decrease in body weight. The BX food-restricted group had to be fed more food to maintain the same weight as the SH-70% hamsters, and the BX-70% group also had a higher core body temperature, lower percent body fat, and higher serum free thyroxine levels than SH food-restricted animals. In summary, removal of the olfactory bulbs appears to facilitate tonic gonadotropin secretion, such that food restriction is no longer capable of inducing testicular regression. In addition, the olfactory bulbs may have a strong influence on metabolic function in golden hamsters.

scholarly journals Acute and Subacute Toxicity Studies of the Ethyl Acetate Soluble Proanthocyanidins of the Immature Inflorescence of Cocos nucifera L. in Female Wistar Rats

2019 ◽  
Vol 2019 ◽  
pp. 1-12
Author(s):  
C. P. Ekanayake ◽  
M. G. Thammitiyagodage ◽  
S. Padumadasa ◽  
B. Seneviratne ◽  
C. Padumadasa ◽  
...  
Keyword(s):  
Body Weight ◽  
Acute Toxicity ◽  
Ethyl Acetate ◽  
Wistar Rats ◽  
Cocos Nucifera ◽  
Toxicity Study ◽  
Immature Inflorescence ◽  
Subacute Toxicity ◽  
Toxicity Studies ◽  
Female Wistar Rats

Ayurvedic and traditional medical practitioners of Sri Lanka use the decoction of the immature inflorescence of Cocos nucifera L. (IC) variety aurantiaca for the treatment of menorrhagia. The progestogenic effect of the ethyl acetate soluble proanthocyanidins (EASPA) of the IC in female rats at a dose of 3.5 mg/kg body weight has been reported. Acute and subacute toxicity studies of EASPA of the IC carried out using female Wistar rats according to Organization for Economic Co-operation and Development (OECD) guidelines 423 and 407, respectively, are reported herein. In the acute toxicity study, a single dose of EASPA (2000 mg/kg body weight) was orally administered to rats, which were monitored for 14 days. In the subacute toxicity study, rats were orally administered with EASPA daily for 28 days at doses of 1.75, 3.5, 7, and 14 mg/kg body weight. No rat in either the acute or subacute toxicity study exhibited mortality or clinical signs of toxicity. Further, these rats did not show any significant change in their mean body weight, food, and water intake, haematological and biochemical parameters as well as in the results of their histopathological examinations compared to those of control group rats. According to results of the acute toxicity, the LD50 of EASPA is estimated to be greater than 2000 mg/kg body weight. Considering the results of the subacute toxicity study, the oral administration of EASPA daily for 28 days was well tolerated up to the dose, 14 mg/kg by rats. These results will be useful in the development of a novel therapeutic agent from EASPA of the IC for the treatment of menorrhagia, which incapacitates a considerable proportion of women worldwide.

Effect of body weight on the attainment of puberty and reproductive performance of Galway and Fingalway female lambs

1979 ◽  
Vol 28 (3) ◽  
pp. 297-307 ◽  
Author(s):  
J. F. Quirke
Keyword(s):  
Body Weight ◽  
Breeding Season ◽  
Litter Size ◽  
Conception Rate ◽  
Restricted Feeding ◽  
Ewe Lambs ◽  
Younger Age ◽  
Body Weights ◽  
The Mean ◽  
Ad Libitum

ABSTRACTGroups of spring-born Galway (G) and Fingalway (F) ewe lambs were fed a concentrate diet either ad libitum (H) or at a restricted level (L) during the period 25 July to 16 October 1974 in order to induce differences in body weight at the start of the breeding season. The mean live weights of the GL, GH, FL and FH groups on 16 October 1974 were 37·8 ± 1·3, 44·6 ± 1·3, 35·1 ± 1·1 and 41·9 ± 1·2 kg respectively. More than 95% of the lambs in all four groups attained puberty before 28 February 1975. Galway ewe lambs reached puberty later in the breeding season and were older and heavier at puberty than Fingalways. Animals fed ad libitum were heavier at puberty and attained puberty earlier in the season and at a younger age than those on the restricted feeding regime.Both conception rate and litter size were lower in Galways than Fingalways. There was no evidence of any effect of the nutritional treatments on conception rate or litter size. The mean body weights at puberty for ewes which lambed and those which were barren were 41·9 ± 0·49 and 41·5 ± 0·78 kg respectively. The birth weight and growth rate of the progeny of the ewe lambs was similar for the two breeds and was not influenced by the previous nutritional treatment of the dams. The total lamb mortality between birth and weaning was 40·3%, and 72% of all deaths occurred within 48 h of birth. Losses among twins (48%) were particularly heavy.

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