scholarly journals Characterization of repetitive DNA sequences carrying 5S rDNA of the triploid ginbuna (Japanese silver crucian carp, Carassius auratus langsdorfi).

1998 ◽  
Vol 73 (1) ◽  
pp. 9-20 ◽  
Author(s):  
Masaru Murakami ◽  
Hideo Fujitani
Keyword(s):  
Repetitive Dna ◽  
Dna Sequences ◽  
Carassius Auratus ◽  
Crucian Carp ◽  
5S Rdna ◽  
Repetitive Dna Sequences ◽  
Silver Crucian Carp ◽  
Crucian Carp Carassius Auratus

scholarly journals Genetic characterization of the progeny of a pair of the tetraploid silver crucian carp Carassius auratus langsdorfii

2013 ◽  
Vol 79 (6) ◽  
pp. 935-941 ◽  
Author(s):  
Jie Dong ◽  
Masaru Murakami ◽  
Takafumi Fujimoto ◽  
Etsuro Yamaha ◽  
Katsutoshi Arai
Keyword(s):  
Carassius Auratus ◽  
Crucian Carp ◽  
Genetic Characterization ◽  
Silver Crucian Carp ◽  
Crucian Carp Carassius Auratus

Purification and characterization of chymotrypsins from the hepatopancreas of crucian carp (Carassius auratus)

2009 ◽  
Vol 116 (4) ◽  
pp. 860-866 ◽  
Author(s):  
Feng Yang ◽  
Wen-Jin Su ◽  
Bao-Ju Lu ◽  
Tao Wu ◽  
Le-Chang Sun ◽  
...  
Keyword(s):  
Carassius Auratus ◽  
Crucian Carp ◽  
Purification And Characterization ◽  
Crucian Carp Carassius Auratus

Wheat specific repetitive DNA sequences ? construction and characterization of four different genomic clones

1986 ◽  
Vol 72 (2) ◽  
pp. 207-210 ◽  
Author(s):  
M. Metzlaff ◽  
W. Troebner ◽  
F. Baldauf ◽  
R. Schlegel ◽  
J. Cullum
Keyword(s):  
Repetitive Dna ◽  
Dna Sequences ◽  
Repetitive Dna Sequences ◽  
Genomic Clones

scholarly journals Repetitive DNA sequences accelerate molecular cytogenetic research in plants with small chromosomes

2019 ◽  
Vol 24 (2) ◽  
pp. 82
Author(s):  
Agus Budi Setiawan ◽  
Ari Wibowo ◽  
Chee How Teo ◽  
Shinji Kikuchi ◽  
Takato Koba
Keyword(s):  
Repetitive Dna ◽  
Dna Sequences ◽  
5S Rdna ◽  
Repetitive Dna Sequences ◽  
Type I ◽  
Chromosome Identification ◽  
Metaphase Chromosomes ◽  
Homologous Chromosomes ◽  
Centromeric Sequence ◽  
Subtelomeric Sequence

Repetitive DNA sequences are highly abundant in plant genomes and are favorable probes for chromosome identification in plants. However, it is difficult to conduct studies on the details of metaphase chromosome structures in plants with small chromosomes due to their highly condensed status. Therefore, identification of homologous chromosomes for karyotyping and analyzing chromosome structures is a challenging issue for cytogeneticists without specific probes and precise chromosome stages. In this study, five repetitive DNA probes, i.e., 5S and 45S ribosomal DNAs (rDNAs), melon centromeric sequence (Cmcent), cucumber subtelomeric sequence (Type I), and microsatellite (CT)10 repeats, were used to identify primary constrictions and homologous chromosomes for karyotyping. Four and two loci of 45S rDNA were respectively observed on metaphase and pachytene chromosomes of Abelia × grandiflora. Cmcent was detected on both primary constrictions of melon pachytene and metaphase chromosomes. Furthermore, one pair of 5S rDNA signals were hybridized on melon metaphase chromosomes. Eight and two loci of 45S and 5S rDNA were respectively detected on cucumber chromosomes. Type I and (CT)10 probes were specifically hybridized on subtelomeric and interstitial regions on the chromosomes, respectively. These results suggest that repetitive DNA sequences are versatile probes for chromosome identification in plants with small chromosomes, particularly for karyotyping analyses.

Variation in highly repetitive DNA composition of heterochromatin in rye studied by fluorescence in situ hybridization

Genome ◽  
1995 ◽  
Vol 38 (6) ◽  
pp. 1061-1069 ◽  
Author(s):  
A. Cuadrado ◽  
N. Jouve ◽  
C. Ceoloni
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Repetitive Dna ◽  
Dna Sequences ◽  
5S Rdna ◽  
Individual Identification ◽  
Highly Repetitive Dna ◽  
The Individual

The molecular characterization of heterochromatin in six lines of rye has been performed using fluorescence in situ hybridization (FISH). The highly repetitive rye DNA sequences pSc 119.2, pSc74, and pSc34, and the probes pTa71 and pSc794 containing the 25S–5.8S–18S rDNA (NOR) and the 5S rDNA multigene families, respectively, were used. This allowed the individual identification of all seven rye chromosomes and most chromosome arms in all lines. All varieties showed similar but not identical patterns. A standard in situ hybridization map was constructed following the nomenclature system recommended for C-bands. All FISH sites observed appeared to correspond well with C-band locations, but not all C-banding sites coincided with hybridization sites of the repetitive DNA probes used. Quantitative and qualitative differences between different varieties were found for in situ hybridization response at corresponding sites. Variation between plants and even between homologous chromosomes of the same plant was found in open-pollinated lines. In inbred lines, the in situ pattern of the homologues was practically identical and no variation between plants was detected. The observed quantitative and qualitative differences are consistent with a corresponding variation for C-bands detected both within and between cultivars.Key words: fluorescence in situ hybridization, repetitive DNA, rye, Secale cereale, polymorphism.

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