scholarly journals Diversity of endophytic fungi from the root bark of Syzygium zeylanicum, and the antibacterial activity of fungal extracts, and secondary metabolite

2021 ◽  
Vol 22 (10) ◽  
Author(s):  
Syarifah Syarifah ◽  
Elfita Elfita ◽  
HARY WIDJAJANTI ◽  
ARUM SETIAWAN ◽  
ALFIA R. KURNIAWATI
Keyword(s):  
Antibacterial Activity ◽  
Secondary Metabolites ◽  
Secondary Metabolite ◽  
Bioactive Compounds ◽  
Molecular Identification ◽  
Endophytic Fungi ◽  
Biological Activities ◽  
Root Bark ◽  
Fungal Extracts

Abstract. Syarifah, Elfita, Widjajanti H, Setiawan A, Kurniawati AR. 2021. Diversity of endophytic fungi from the root bark of Syzygium zeylanicum, and the antibacterial activity of fungal extracts, and secondary metabolite. Biodiversitas 22: 4572-4582. The decoction of the root bark of Syzygium zeylanicum has been used as traditional medicine, such as for treating pathogenic bacterial infections. Endophytic fungi that live in medicinal plant tissues have a high species diversity and biological activities correlate with their host. Therefore, this study aimed to explore the diversity of endophytic fungi from the root bark of S. zeylanicum and to determine the antibacterial activity of endophytic fungi and their secondary metabolites. In this study, we isolate and identify the endophytic fungi from the root bark of S. zeylanicum, continued by screening their antibacterial activity against two Gram-negative bacteria (Escherichia coli InaCCB5 and Salmonella thypi ATCC1048 and two Gram-positive bacteria (Staphylococcus aureus InaCCB4 and Bacillus subtilis InaCCB1204) by the Kirby-Bauer method. The fungal extract with the highest antibacterial activity proceeded with the isolation and determination of the structure of their bioactive compounds. The isolates were morphologically identified. Isolates that showed strong antibacterial activity were identified by molecular identification. Isolation of bioactive compounds was carried out by chromatographic techniques and the determination of the structure of pure chemical compounds was performed by the spectroscopic analysis. In total, there were 8 isolates of endophytic fungi were obtained from the root bark of S. zeylanicum, namely SZR1 – SZR8. SZR2 isolate has the highest antibacterial activity. Molecular identification through phylogenetic analysis showed that SZR2 isolate had high similarity with Penicillium brefeldianum. Isolation of bioactive compounds from SZR2 produced compound 1 in the form of light yellow crystals which showed strong antibacterial activity against S. typhi, E. coli, and B. subtilis with MIC values of 64 g/mL. Compound 1 was identified as p-hydroxybenzaldehyde, which was also obtained in its host. In conclusion, the endophytic fungus Penicillium brefeldianum produces similar secondary metabolites and antibacterial activity as its host plant.

scholarly journals Evaluation of antimicrobial, antioxidant and cytotoxic properties of bioactive compounds produced from endophytic fungi of Himalayan yew (Taxus wallichiana) in Nepal

F1000Research ◽  
2020 ◽  
Vol 9 ◽  
pp. 379 ◽  
Author(s):  
Dhurva Prasad Gauchan ◽  
Pratistha Kandel ◽  
Astha Tuladhar ◽  
Ashesh Acharya ◽  
Upendra Kadel ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Secondary Metabolites ◽  
Ethyl Acetate ◽  
Bioactive Compounds ◽  
Endophytic Fungi ◽  
Diffusion Method ◽  
Dry Extract ◽  
Brine Shrimp Lethality Assay ◽  
Fungal Extracts ◽  
Taxus Wallichiana

Background: Endophytic fungi are largely underexplored in the discovery of natural bioactive products though being rich sources of novel compounds with promising pharmaceutical potential. In this study, Taxus wallichiana, which has huge medicinal value, was investigated for its endophytic diversity and capability to produce bioactive secondary metabolites by analyzing antioxidant, antimicrobial and cytotoxic properties. Methods: The endophytes were identified by ITS-PCR using genomic DNA samples. The secondary metabolites were extracted by solvent extraction method using ethyl acetate. The antioxidant activity was analyzed by Thin Layer Chromatography, Total Phenol Content (TPC), Total Flavonoid Content (TFC) and DPPH assay, and the antimicrobial activity was analyzed by agar-well diffusion method. Brine shrimp lethality assay was used to analyze the cytotoxicity of the fungal extracts. Results: Out of 16 different Taxus trees sampled from different locations of Dhorpatan, 13 distinctive endophytic fungi were isolated and grouped into 9 different genera: Bjerkandera, Trichoderma, Preussia, Botrytis, Arthrinium, Alternaria, Cladosporium, Sporormiella and Daldinia. The ethyl acetate extracts isolated from three endophytic fungi: Alternaria alternata, Cladosporium cladosporioides and Alternaria brassicae showed significant TPC values of 204±6.144, 312.3±2.147 and 152.7±4.958µg GAE/mg of dry extract, respectively, and TFC values of 177.9±2.911, 644.1±4.202 and 96.38±3.851µg RE/mg of dry extract, respectively. Furthermore, these three extracts showed a dose dependent radical scavenging activity with IC50 concentration of 22.85, 22.15 and 23.001 µg/ml, respectively. The extracts of C. cladosporioides and A. brassicae also showed promising antimicrobial activity against Escherichia coli, Staphylococcus aureus and Bacillus subtilis with a minimum inhibitory concentration of 250μg/ml for all bacteria. Both the samples showed cytotoxic property against shrimp nauplii with LC50 of 104.2 and 125.9µg/ml, respectively. Conclusions: The crude fungal extracts obtained from endophytes: A. alternata, C. cladosporioides and A. brassicae upon purification and further identification of the bioactive compounds can be a fascinating source for novel pharmaceutical agents.

scholarly journals Determination of Biological Activities of the Root Bark of Senna singueana

2020 ◽  
pp. 25-34
Author(s):  
Kidanemariam Teklay Hilawea ◽  
Zelalem Yibralign Desta
Keyword(s):  
Antibacterial Activity ◽  
Biological Activities ◽  
Total Flavonoid ◽  
Diffusion Method ◽  
Total Phenolic ◽  
Phytochemical Analysis ◽  
Root Bark ◽  
Total Flavonoid Content ◽  
Flavonoid Content

Aims: Senna singueana is a medicinal plant which is used for the treatment of different kinds of diseases and the plant was selected for the study because of its numerous uses. The main objective of this study was to determine the antioxidant and antibacterial activity of the extracts of the root barks of S. singueana. Methodology: The antibacterial activities of the extracts (determination of Minimal Inhibitory “MIC” and Minimal Bactericidal Concentration “MBC”) were determined by using agar well diffusion method. In addition to this the total flavonoid and total phenolic contents were determined by using aluminum chloride colorimetric complex assay and Folin-Ciocalteu method respectively. Results: Our results revealed that the total flavonoid content of the extracts is ranged from 30.39 mgQE/100 g to 240.83 mgQE/100 g. The extracts also showed good antioxidant activity and total phenolic content as well as weak to moderate antibacterial activity against some bacteria. Conclusions: The extracts of the root bark of Senna sinueana showed good total flavonoid content, DPPH radical scavenging activity and antibacterial activity. In addition to this, the extracts also showed the presence of some important compounds by phytochemical analysis.

scholarly journals EXPLORATION OF ENDOPHYTIC FUNGI OF DRAGON SCALE’S FERN (Pyrrosia piloselloides (L.) M.G. Price) AS AN ANTIBACTERIAL SOURCES

2019 ◽  
Vol 5 (2) ◽  
Author(s):  
Angga Puja Asiandu ◽  
Hary Widjajanti ◽  
Elisa Nurnawati
Keyword(s):  
Antibacterial Activity ◽  
Secondary Metabolites ◽  
Host Plant ◽  
Bioactive Compounds ◽  
Endophytic Fungi ◽  
Host Plants ◽  
Inhibitory Concentration ◽  
Inhibition Zone ◽  
Pyrrosia Piloselloides ◽  
Ethyl Acetate Extracts

Endophytic fungi are fungi which live inside the host plant tissue and have been undergone a horizontal gene transfer process. Endophytic fungi are able to synthesize the same bioactive compounds which synthesized by their host plants. The host plant used in this research was dragon’s scales fern (Pyrrosia piloselloides (L.) M.G. Price). Dragon’s scales fern produces various of bioactive compounds which used as antibacterial agents such as polyphenols. This research was aimed to obtain endophytic fungi isolates from trophophyll fronds and sporophyll fronds of dragon’s scales fern, to determine the antibacterial activity of the secondary metabolite extracts of endophytic fungi, to determine the Minimum Inhibitory Concentration (MIC), to determine the characteristics of the endophytic fungi isolates which potentially as antibacterial source. Based on the research, 13 endophytic fungi isolates were obtained from dragon’s scales fern fronds consist of 5 isolates from trophophyll fronds and 8 isolates from sporophyll fronds. The antibacterial activity test showed that the extract of secondary metabolites of the isolate DTP2 had the highest inhibition zone diameter against E.coli 14.82 ± 4.05 mm, DTP4 against S.aureus 8.80 ± 0.03 mm and DSP4 against S.dysentriae 10.15 ± 0.36 mm. MIC of ethyl acetate extracts of secondary metabolites of isolate DTP2 against E.coli was 125 µg/mL, DTP4 against S.aureus was 125 µg/mL and DSP4 against S.dysentriae was 31.25 µg/mL. The endophytic fungi isolate DTP2 identified as Aureobasidium melanogenum, DTP4 identified as Penicillium alliisativi and DSP4 identified as Aspergillus flocculosus.

scholarly journals Evaluation of antimicrobial, antioxidant and cytotoxic properties of bioactive compounds produced from endophytic fungi of Himalayan yew (Taxus wallichiana) in Nepal

F1000Research ◽  
2020 ◽  
Vol 9 ◽  
pp. 379
Author(s):  
Dhurva Prasad Gauchan ◽  
Pratistha Kandel ◽  
Astha Tuladhar ◽  
Ashesh Acharya ◽  
Upendra Kadel ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Secondary Metabolites ◽  
Ethyl Acetate ◽  
Bioactive Compounds ◽  
Endophytic Fungi ◽  
Diffusion Method ◽  
Dry Extract ◽  
Brine Shrimp Lethality Assay ◽  
Fungal Extracts ◽  
Taxus Wallichiana

Background: Endophytic fungi are largely underexplored in the discovery of natural bioactive products though being rich sources of novel compounds with promising pharmaceutical potential. In this study, Taxus wallichiana, which has huge medicinal value, was investigated for its endophytic diversity and capability to produce bioactive secondary metabolites by analyzing antioxidant, antimicrobial and cytotoxic properties. Methods: The endophytes were identified by ITS-PCR using genomic DNA samples. The secondary metabolites were extracted by solvent extraction method using ethyl acetate. The antioxidant activity was analyzed by Thin Layer Chromatography, Total Phenol Content (TPC), Total Flavonoid Content (TFC) and DPPH assay, and the antimicrobial activity was analyzed by agar-well diffusion method. Brine shrimp lethality assay was used to analyze the cytotoxicity of the fungal extracts. Results: Out of 16 different Taxus trees sampled from different locations of Dhorpatan, 13 distinctive endophytic fungi were isolated and grouped into 9 different genera: Bjerkandera, Trichoderma, Preussia, Botrytis, Arthrinium, Alternaria, Cladosporium, Sporormiella and Daldinia. The ethyl acetate extracts isolated from three endophytic fungi: Alternaria alternata, Cladosporium cladosporioides and Alternaria brassicae showed significant TPC values of 204±6.144, 312.3±2.147 and 152.7±4.958µg GAE/mg of dry extract, respectively, and TFC values of 177.9±2.911, 644.1±4.202 and 96.38±3.851µg RE/mg of dry extract, respectively. Furthermore, these three extracts showed a dose dependent radical scavenging activity with IC50 concentration of 22.85, 22.15 and 23.001 µg/ml, respectively. The extracts of C. cladosporioides and A. brassicae also showed promising antimicrobial activity against Escherichia coli, Staphylococcus aureus and Bacillus subtilis with a minimum inhibitory concentration of 250μg/ml for all bacteria. Both the samples showed cytotoxic property against shrimp nauplii with LC50 of 104.2 and 125.9µg/ml, respectively. Conclusions: The crude fungal extracts obtained from endophytes: A. alternata, C. cladosporioides and A. brassicae upon purification and further identification of the bioactive compounds can be a fascinating source for novel pharmaceutical agents.

scholarly journals Diversity, Chemical Constituents and Biological Activities of Endophytic Fungi Isolated from Schinus terebinthifolius Raddi

2020 ◽  
Vol 8 (6) ◽  
pp. 859
Author(s):  
Paola dos Santos da Rocha ◽  
Vanessa Marina Branco Paula ◽  
Silvia Cristina Figueira Olinto ◽  
Edson Lucas dos Santos ◽  
Kely de Picoli Souza ◽  
...  
Keyword(s):  
Secondary Metabolites ◽  
Endophytic Fungi ◽  
Biological Activities ◽  
Chemical Constituents ◽  
Fungal Growth ◽  
Stem Bark ◽  
Antibacterial Activities ◽  
Schinus Terebinthifolius ◽  
Hydroxybenzoic Acids ◽  
Fungal Extracts

Schinus terebinthifolius Raddi is a medicinal plant widely used for the treatment of various diseases. The secondary metabolites responsible for the pharmacological properties can be produced directly by the plant or by endophytic fungi. The objective of this study was to evaluate the diversity of endophytic fungi of different parts of S. terebinthifolius and to identify chemical compounds produced by endophytes and their antioxidant and antibacterial activities. For this, fruits, stem bark and roots were dried, ground and placed in fungal growth medium. The selected endophytes were grown and subjected to extraction with ethyl acetate. DPPH, FRAP, β-carotene bleaching and antimicrobial assays were performed. The phylogenetic tree was elaborated, encompassing 15 different species. The fungal extracts showed hydroxybenzoic acids and 1-dodecanol as predominant compounds. All fungal extracts exhibited antioxidant activity. The fungal extracts exhibited bactericidal and bacteriostatic activities against Gram-positive and Gram-negative bacterial ATCC strains and against methicillin-resistant nosocomial bacteria. Among the 10 endophytic fungi evaluated, the extract of the fungus Ochrocladosporium elatum showed higher phenolic content and exhibited higher antioxidant and antibacterial activities in all tests. Together, the results increase the known diversity of S. terebinthifolius endophytic fungi, secondary metabolites produced and their antioxidant and antibacterial activities.

scholarly journals In-Vitro Antibacterial Activity of Endophytic Fungi from Peganum harmala (Laghouat, Algeria)

2020 ◽  
Vol 10 (3-s) ◽  
pp. 47-51
Author(s):  
Yasmina Ouzid ◽  
Siliya Karaoui ◽  
Noria Smail Saadoun ◽  
Karim Houali
Keyword(s):  
Antibacterial Activity ◽  
Secondary Metabolites ◽  
Endophytic Fungi ◽  
Biological Activities ◽  
Significant Activity ◽  
Bacterial Strains ◽  
Peganum Harmala ◽  
Bioactive Substances ◽  
Significant Difference

Medicinal plants are an inexhaustible source of molecules. They are colonized by mycoendophytes, fungi living in their tissues without apparent symptoms. These fungi can provide secondary metabolites with biological activities. It is with this in mind that we are interested in a spontaneous plant from the dayas region (Laghouat, Algeria): Peganum harmala or Harmel, a toxic medicinal plant belonging to the family Zygophyllaceae. Our study consists in highlighting the antibacterial activity of four kinds of mycoendophytes: Cladosporium, Alternaria, Aspergillus and Penicillium isolated from the leaves of this plant. The antibacterial activity is evaluated by the technique of the double disk diffusion on agar with respect to some Gram-positive bacterial strains. We have adopted two protocols for this purpose. For the first, the mycelia of all the mushrooms are deposited in the same petri dish. For the second, a single disc of the mycelium of a single species is deposited per box. The results obtained show a difference in the sensitivity of the bacterial strains to the bioactive substances of the mycoendophytes studied. The Alternaria genus showed the most significant activity. ANOVA performed between the mean diameters of the mycoendophyte inhibition zones and the antibiotic test disc: Chloramphenicol showed a highly significant difference between these two measurements. The Newman-Keuls test revealed a difference in the susceptibility of bacterial strains to the secondary metabolites of fungus mycoendophytes of Peganum harmala according to the two protocols used. The antibacterial effect is related to interactions between endophytic fungi and their host plant.

Bioactive Secondary Metabolites from Endophytic Fungi

2020 ◽  
Vol 27 (11) ◽  
pp. 1836-1854 ◽  
Author(s):  
Elena Ancheeva ◽  
Georgios Daletos ◽  
Peter Proksch
Keyword(s):  
Secondary Metabolites ◽  
Endophytic Fungi ◽  
Host Plants ◽  
Biological Activities ◽  
Cytotoxic Agents ◽  
Special Focus ◽  
Plant Metabolites ◽  
Direct Production ◽  
Bioactive Secondary Metabolites ◽  
Fungal Genes

Background: Endophytes represent a complex community of microorganisms colonizing asymptomatically internal tissues of higher plants. Several reports have shown that endophytes enhance the fitness of their host plants by direct production of bioactive secondary metabolites, which are involved in protecting the host against herbivores and pathogenic microbes. In addition, it is increasingly apparent that endophytes are able to biosynthesize medicinally important “phytochemicals”, originally believed to be produced only by their host plants. Objective: The present review provides an overview of secondary metabolites from endophytic fungi with pronounced biological activities covering the literature between 2010 and 2017. Special focus is given on studies aiming at exploration of the mode of action of these metabolites towards the discovery of leads from endophytic fungi. Moreover, this review critically evaluates the potential of endophytic fungi as alternative sources of bioactive “plant metabolites”. Results: Over the past few years, several promising lead structures from endophytic fungi have been described in the literature. In this review, 65 metabolites are outlined with pronounced biological activities, primarily as antimicrobial and cytotoxic agents. Some of these metabolites have shown to be highly selective or to possess novel mechanisms of action, which hold great promises as potential drug candidates. Conclusion: Endophytes represent an inexhaustible reservoir of pharmacologically important compounds. Moreover, endophytic fungi could be exploited for the sustainable production of bioactive “plant metabolites” in the future. Towards this aim, further insights into the dynamic endophyte - host plant interactions and origin of endophytic fungal genes would be of utmost importance.

scholarly journals Bitkilerde Bulunan Fitokimyasalların Biyolojik Aktiviteleri

2020 ◽  
Vol 8 (8) ◽  
pp. 1734-1746
Author(s):  
Tuğba Demir ◽  
Özlem Akpınar
Keyword(s):  
Secondary Metabolites ◽  
Chronic Diseases ◽  
Bioactive Compounds ◽  
Biological Activities ◽  
Beneficial Effects ◽  
Synthetic Materials ◽  
Effective Role ◽  
Materials Used ◽  
Antihypertensive Properties

Bioactive compounds, called phytochemicals, are produced as secondary metabolites in plants that have beneficial effects on health when they are consumed as nutrients. Phytochemicals have an effective role in the formation of the color, smell and taste of the plants. As an alternative to the synthetic materials used in the treatment of many chronic diseases, the interest in the use of plants phytochemicals have been increased. This trend has led to the development of a new market. This review includes biological activities of plant phytochemicals including antioxidant, antimicrobial, antifungal, antidiabetic, antiinflammatory, anticancer and antihypertensive properties.

scholarly journals Malagasy Liverworts, Source of New and Biologically Active Compounds

2007 ◽  
Vol 2 (6) ◽  
pp. 1934578X0700200 ◽  
Author(s):  
Liva Harinantenaina ◽  
Yoshinori Asakawa
Keyword(s):  
Antibacterial Activity ◽  
Secondary Metabolites ◽  
Aromatic Compounds ◽  
Biological Activities ◽  
Biologically Active ◽  
Raw 264.7 Cells ◽  
No Production ◽  
Raw 264.7 ◽  
Strong Inhibition ◽  
Phytochemical Investigation

The phytochemical investigation of eight Jungermaniales liverwort species: Bazzania decrescens, B. madagassa (Lepidoziaceae), Plagiochila barteri, P. terebrans (Plagiochilaceae), Isotachis aubertii (Isotachidaceae), Mastigophora diclados (Lepicoleaceae), Radula appressa (Radulaceae), and Thysananthus spathulistipus (Lejeuneaceae), collected from Madagascar, has been carried out to afford new and structurally interesting terpenoids and aromatic compounds. The biological activities of the isolated secondary metabolites were determined and the herbertene-type sesquiterpenoids were shown to have antibacterial activity. A new ent-clerodane diterpene from Thysananthus spathulistipus and bis-bibenzyls-type aromatic compounds exhibited strong inhibition of NO production in RAW 264.7 cells, while marchantin C produced moderate α-glucosidase inhibition. The chemosystematics of the studied species are discussed.

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