GERMINATION OF PORANG (Amorphophalus muelleri) FROM DIFFERENT BULBIL TO VARIOUS PLANTING MEDIA

Porang plant is atubers that havevarious benefits. Porang cultivation needs intensive management through cultivation efforts which of course must be supported by the availability of seeds. Therefore, it is necessary to procure porang seeds from vegetative (tubers) and generative (stem bulbil and branch bulbil). This study aims to obtain the best growing media for porang germination from various types of bulbil. The method used in this study was a factorial randomized block design. The way of working in this research is: Preparation of Seedling Media, Preparation of planting material (Bulbil), Seeding, Maintenance (including watering and weeding). The results showed that there was no significant interaction between the planting medium and the type of bulbil, the media had no significant effect on all observed variables, while the bulbil had a significant effect on the percentage of germination growth, stem height, and number of leaves. The best type of bulbil for breeding is stem bulbil which is superior to the variables of plumule emergence time, petiole length, stem height, and number of leaves compared to branch bulbil. Meanwhile, the best growing media in porang nurseries was a mixture of 50% husk charcoal + 50% sawdust on the variables when the plumule appeared, the percentage of germination, petiole length and stem height. The appearance of bulbil from stems on a mixture of 50% husk charcoal + 50% sawdust media, namely the time of emergence of plumules (5.61 days), percentage of sprouting (86.67%), petiole length (8.72 cm), height stems (31.11 cm) and number of leaves (7.72 strands).

HIPPOCAMPUS CELL DISORDERS AND NEUROSENSORY TESTS IN MICE (Mus musculus) DUE TO INDUCTION OF EXCESS SODIUM CHLORIDE

Salt or sodium chloride (NaCl) is an additive to give food a salty taste. The use of salt in everyday life is difficult to avoid. Salt has both good and bad effects on the body. The electrolyte content of salt can help launch metabolism in the body, whereas if the body contains too much salt it will cause heart attacks and hypertension. The dose of salt consumption that has been set by the government is 5g/day. This study was conducted to examine the effect of consuming excessive sodium chloride (NaCl) on the ability to smell and to what extent it damages cells in the hippocampus of mice (Mus musculus). The method of this study was CRD (completely randomized design) with 6 replications, 1 group control and 3 repetitions induced by sodium chloride (NaCl) is 260mg/gBB (P1), 520mg/gBB (P2), and 780mg/gBB (P3). The parameters of this study are neurosensory coordination in the form of olfactory response of mice (Mus musculus) to ammonia and cell disruption in the hippocampus (DG & CA) which were observed by histological preparations of Hematoxylin eosin (HE) staining. The results of this study indicate the presence of olfactory disorders in mice (Mus musculus) and cell death in the hippocampus also increased due to excessive sodium chloride (NaCl) induction. The more salt is consumed in daily life, it will disrupt the cells in the hippocampus.

THE APPLICATION OF DRY RUBBER LATEX AND VERMICOMPOST TO PLANT WATER CONSUMPTION AND THE GROWTH OF PADDY ON SWAMPY LAND

Swampy land is an alternative for rice plant cultivation where the amount of rainfall has an impact on the amount of inundation. In order to provide appropriate water for rice plants, especially during the dry season, an irrigation water system is required for its control.Alternative materials such as dry rubber latex and vermicom-post can be utilized to reduce water use and ensure that water is delivered as efficiently as possible. The goal of this study was to see how dried rubber latex on the soil surface and vermicompost in the soil affected rice plant water demand and growth. This research was conducted from November 2020 – February 2021. The study used a factorial randomized block design (RAKF) with 2 treatments, covered dry rubber latex variations (K), K0 (0%), K1 (25%), K2 (50%), K3 (75%), and vermicompos (V), V0(0 g), V1(58.82 g), V2(117.64 g), V3 (176.46 g)with 3 rep-licat. So that the total treatmentsare 48 treatments.The results showed that the application of dry rubber latex and vermicompost significantly affected the number of leaves and the number of tillers. The K3 treatment (75%) was the best because it had the smallest evapotranspiration rate with an average of 1.2 mm day-1. The K3 treatment (75%) had the smallest average water requirement of 1.6 mm day-1 with an average number of 37.07 leaves. The high yield of K3 treatment was due to the wider the area covered by 75% (K3), the higher the water content, nutrients, and the lower the soil temperature. Closure using dry rubber latex can reduce sunlight so that the process of water loss is lower, and the available nutrients are higher.

INNOVATION OF SINBIOTIC FORMULA FOR THE GROWTH OF WHITE SHRIMP LARVAE (Litopenaeus vannamei)

In the hatchery of white shrimp larvae, there are some problems, such as a decreased water quality and diseases caused by bacteria which cause a decrease in shrimp growth. The solution to reduce these problems is by applying synbiotics. This research aimed to make an innovative synbiotic formula for the absolute length growth and survival rate of white shrimp larvae (Litopenaeus vannamei) as well as the total number of bacteria and the number of Vibrio sp. In this study, there were four treatments, as follows: C-: negative control (without synbiotic application) C+: positive control (commercial synbiotic) P: probiotic 2.5 x 105 cfu/ml and prebiotic 1,5 ppm Q: probiotic 5 x 105 cfu/ml and prebiotic 1 ppm R: probiotic 7,5 x 105 cfu/ml and prebiotic 0,5 ppm S: probiotic 106 cfu/ml and prebiotic 2 ppm, each treatment with four repetitions. This research was set up in a completely randomized design experiment using twenty-four plastic tanks with 50 L total volume filled with 20 L sterile sea water and stocked with 4,000 nauplii in the PT. Citra Larva Cemerlang hatchery, Kalianda, Lampung. Variables observed in this research were survival rate, absolute length growth, total bacterial and Vibrio sp. counts, and water quality in the white shrimp larvae (Litopenaeus vannamei). The results showed that the best survival rate is in treatment S (probiotic 106 cfu/ml and prebiotic 2 ppm) (87.7%), the highest absolute length growth is in treatment P (3.8 mm), the highest total bacteria was in treatment S (11.1 log cfu/ml), and the best total Vibrio sp. is in treatment S (3.5 log cfu/ml). Water quality of the six treatments shows results that are still in normal conditions following Indonesian National Standard SNI 7311: 2009.

THREE SPOT GOURAMI (Trichogaster trichopterus) FOOD HABITS IN THE KOMERING RIVER'S DOWNSTREAM, SOUTH SUMATERA

One of the downstream areas of the Komering River with potential for capture fisheries is Sungai Dua Village. One of the types of fish caught by fishermen in Sungai Dua is the three spot gourami (Trichogaster trichopterus). The three spot gourami fish is a type of freshwater consumption fish that has high economic value. But the fishes only rely on catches from nature. If the fishing is carried out continuously, without domestication and cultivation, it will cause a decline in the fish population. The research about the biological aspects of red-eye septic fish (Trichogaster trichopterus) which include food habits, relationship length and weight, condition factors and stomach fullness index. This research was conducted from April to June 2017. Sampling was carried out in the downstream waters of the Komering River in Sungai Dua Village, Banyuasin Regency, South Sumatra. Fish samples were analyzed at the Animal Taxonomy Laboratory, Department of Biology, Faculty of Mathematics and Science, Sriwijaya University. The determination of stations in sampling was carried out using multiple sampling methods, namely purposive sampling and quota sampling. The result of this research showed that (1) the Cyanophyta is the main food for the three spot gourami, Diatomae and Desmidiacae as a complementary food, while the additional foods are Rhizopoda, Chlorophyta, Ostracoda, and Entomostraca, (2) The growth pattern of red-eyed fish is negative allometric with a b value of -2 (b <3), the increase in length is faster than the weight gain, (3) The highest condition factor value is found in the size range 6.01-7.00 cm of 23.25 gr / cm and the lowest condition factor value is in the size range 5.01-6.00 cm of 0.62 gr / cm, and (4) The highest hull fullness index value of the red eye sepat fish hull is in the size range 7.01-8.00 cm at 32.86% and the lowest hull fullness index value at the size range 8.01-9.00 cm is 2.05% .

THE EFFECT OF CULTURE MEDIA TYPE AND PLANT GROWTH REGULATORS ON CALLUS INDUCTION OF OIL PALM (Elaeis guineensis Jacq) PISIFERA TYPE

Oil palm has a high economic value since it is one of the highest vegetable oil-producing plants compared to other oil-producing plants. The provision of good planting material for oil palm plantations is one of the determining factors to increase productivity. The parent Pisifera type of oil palm is important to develop the high-yielding oil palm Tenera varieties. The provision of oil palm material could be done through generative system with seeds and also could be done through vegetative approaches using tissue culture techniques. It aims to get plants that are genetically the same as their parents (true to type). The purpose of this study was to see the effect of different type of tissue culture media and plant growth regulators (PGR’s) on the callus induction stage of Pisifera type oil palm. The results show that the treatment using Murasige and Skoog (MS) culture media is able to give a better effect when compared to the use of Eeuwens and Blake (Y3) basic media. While the use of PGR treatment (H6 = NAA 6 mg/l + 2.4-D 0.5 mg/l) showed a callus growth percentage of 76%, which was better than (H1 = Kin 0.1 mg/l + 2.4-D 100 mg/l) by 42%.

SYNERGISM BETWEEN RHIZOSPHERE BACTERIA ISOLATES FROM Scleria sp., Clidemia sp., AND Panicum sp. TO INCREASE THE EFFECTIVENESS OF MIXED CULTURES IN HYDROCARBON BIODEGRADATION

The purpose of this research is to obtain hydrocarbon degrading bacteria that work synergistically in a consortium. Consortium microorganisms is mixture of microbial populations in the form of communities that have mutualistic relationships and doesn’t inhibition the growth of other microbes. In this study, isolates were obtained from the rhizosphere of soil contaminated with petroleum. The isolates obtained were tested for synergism to determine the relationship between bacterial isolates. Synergism testing was carried out using the spread plate method on agar media. The results of this study showed that isolate number one showed antagonistic properties to other bacterial isolates by forming a clear zone around the disc paper. A total of eight bacterial isolates showed the greatest percentage of synergism, namely ≥ 80% so that the eight rhizosphere bacterial isolates could be used as materials for mixed culture.

EXTRACTION AND PURITY DNA OF Culex spp MOSQUITO IN KEMELAK VILLAGE, BINDUNG LANGIT, OGAN KOMERING ULU

Culex spp are mosquito vectors that have a very wide distribution capability and are carriers of pathogens that can interfere with human and animal health. The wide distribution makes Culex spp a dangerous threat. DNA extraction is one of the important steps in obtaining genetic information and genetic analysis. Good quality DNA is used for activities such as the use of molecular markers, genome library creation, and sequencing. This study aims to determine the quality, concentration and purity of Culex spp mosquito DNA in Kemelak Bindung Langit Village, OKU Regency. It is hoped that the sample can be used for further research analysis on Mitochondria D-Loop Sequences in Culex spp mosquitoes. Quantitative measurement of DNA in the form of concentration and purity of DNA using Nanodrop Thermo cycle while qualitative DNA using electrophoresis technique. The results of the isolation of the mosquito genome DNA, obtained clear DNA bands without any degradation (smear) and the concentration results for the four samples ranged from 10-100 ng/µL and the DNA purity was good, ranging from 1.8 to 2.00.

EFFECT OF WATER CONTENT AND SOIL IMPROVEMENT (HYDROGEL) ON PEAT FIRE SUPPRESSION

Forest and land fires are Indonesia's biggest problem which has continued from 2014 to the present. The number of activities to find the best solution in fire is something that has been done until now. Various methods, both preventive and repressive, have been implemented to prevent forest and land fires from occurring. This research aims to be a sureextinguishing gel, namely hydrogel, which can be used in efforts to extinguish forest and land fires. Extinguishing forest and land fires using hydrogel is a new method that is expected to prevent forest and land fire zones from spreading. This research shows that the tendency of decreasing the average water content of peat due to the drying process based on different intervals of oven time, namely the lowest yield ranged from 61.25% to the highest with a water content of 109.57%.

Morphological Characteristics and Pleasant Relationship Between Crossing of Black Rice Accesion with Inpara 5 that Containing SUB-1 Genes

In South Sumatra the cultivation of black rice is uncommon. This is because of the lack of available land for planting which can be used to grow local black rice. Since more farmers dominate the white rice or brown rice cultivation. One effort that can be made to increase the area of local black rice planting areas is to use South Sumatra's vast swampy land. Related to the previous study, this research will be carried out by testing the growth of F1 seeds crossed by accession to high productivity local black rice and nutrition with marinated resistant Inpara 5 varieties. In quantitative parameters, the maximum average value of it was shown at the age of 12 MST plants that IM.6 plants at 145.08 as the highest average value of the crossing results. IM.6 was the highest median value with 144.5. The maximum standard deviation value was 5.06 for IT.11 plants, and the minimum value being 141.4 for IM.6 forms and 150.0.6 for IM.6 plants. The qualitative parameters consist of plant shape, stem color, leaf color, and flag leaf are seen when the plant is 6 MST or in the vegetative phase. Done by describing plants from 5 sample plants per randomly selected population. From the result of this research, there is no significant differential between accession and parental.