scholarly journals Depth-varying Density and Organization of Chondrocytes in Immature and Mature Bovine Articular Cartilage Assessed by 3D Imaging and Analysis

2005 ◽  
Vol 53 (9) ◽  
pp. 1109-1119 ◽  
Author(s):  
Kyle D. Jadin ◽  
Benjamin L. Wong ◽  
Won C. Bae ◽  
Kelvin W. Li ◽  
Amanda K. Williamson ◽  
...  
Keyword(s):  
Articular Cartilage ◽  
3D Imaging ◽  
Articular Surface ◽  
Three Dimensional ◽  
Neighboring Cell ◽  
3D Images ◽  
Bovine Articular Cartilage ◽  
Stages Of Growth ◽  
Cell Organization ◽  
Heterogeneous Tissue

Articular cartilage is a heterogeneous tissue, with cell density and organization varying with depth from the surface. The objectives of the present study were to establish a method for localizing individual cells in three-dimensional (3D) images of cartilage and quantifying depth-associated variation in cellularity and cell organization at different stages of growth. Accuracy of nucleus localization was high, with 99% sensitivity relative to manual localization. Cellularity (million cells per cm3) decreased from 290, 310, and 150 near the articular surface in fetal, calf, and adult samples, respectively, to 120, 110, and 50 at a depth of 1.0 mm. The distance/angle to the nearest neighboring cell was 7.9 μm/31°, 7.1 μm/31°, and 9.1 μm/31° for cells at the articular surface of fetal, calf, and adult samples, respectively, and increased/decreased to 11.6 μm/31°, 12.0 μm/30°, and 19.2 μm/25° at a depth of 0.7 mm. The methodologies described here may be useful for analyzing the 3D cellular organization of cartilage during growth, maturation, aging, degeneration, and regeneration.

scholarly journals Preoperative Evaluation and Surgical Simulation for Osteochondritis Dissecans of the Elbow Using Three-Dimensional MRI-CT Image Fusion Images

Diagnostics ◽  
2021 ◽  
Vol 11 (12) ◽  
pp. 2337
Author(s):  
Sho Kohyama ◽  
Yasumasa Nishiura ◽  
Yuki Hara ◽  
Takeshi Ogawa ◽  
Akira Ikumi ◽  
...  
Keyword(s):  
Articular Cartilage ◽  
Osteochondritis Dissecans ◽  
Surgical Simulation ◽  
Articular Surface ◽  
Preoperative Evaluation ◽  
Three Dimensional ◽  
Resonance Imaging ◽  
Structural Details ◽  
Fusion Images ◽  
Axial Traction

We used our novel three-dimensional magnetic resonance imaging-computed tomography fusion images (3D MRI-CT fusion images; MCFIs) for detailed preoperative lesion evaluation and surgical simulation in osteochondritis dissecans (OCD) of the elbow. Herein, we introduce our procedure and report the findings of the assessment of its utility. We enrolled 16 men (mean age: 14.0 years) and performed preoperative MRI using 7 kg axial traction with a 3-Tesla imager and CT. Three-dimensional-MRI models of the humerus and articular cartilage and a 3D-CT model of the humerus were constructed. We created MCFIs using both models. We validated the findings obtained from the MCFIs and intraoperative findings using the following items: articular cartilage fissures and defects, articular surface deformities, vertical and horizontal lesion diameters, the International Cartilage Repair Society (ICRS) classification, and surgical procedures. The MCFIs accurately reproduced the lesions and correctly matched the ICRS classification in 93.5% of cases. Surgery was performed as simulated in all cases. Preoperatively measured lesion diameters exhibited no significant differences compared to the intraoperative measurements. MCFIs were useful in the evaluation of OCD lesions and detailed preoperative surgical simulation through accurate reproduction of 3D structural details of the lesions.

scholarly journals A Review on Dual-Lens Fluorescence Microscopy for Three-Dimensional Imaging

2020 ◽  
Vol 8 ◽  
Author(s):  
Xiaoyan Li ◽  
Yubing Han ◽  
Wenjie Liu ◽  
Cuifang Kuang ◽  
Xu Liu ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
3D Imaging ◽  
Signal To Noise Ratio ◽  
Three Dimensional ◽  
Super Resolution ◽  
Light Sheet ◽  
Animal Cells ◽  
3D Images ◽  
Single Lens ◽  
Model Animal

Three-dimensional (3D) imaging using dual-lens fluorescence microscopies is popular in observing fluorescently labeled biological samples, such as mammalian/model animal cells, tissues, and embryos. Specifically, dual-lens super-resolution fluorescence microscopy methods using two opposing objective lenses allow significantly higher axial resolution and better signal to noise ratio than traditional single-lens counterparts, and thus distinguish more details in 3D images of fine intracellular structures. For 3D imaging of thick tissues and entire embryos, dual-lens light-sheet fluorescence microscopy methods using two objective lenses, either orthogonal or non-orthogonal, to achieve selective plane illumination, can meet the requirements, and thus can be used to observe embryo development and structures of interest in thick tissues. This review summarizes both dual-lens fluorescence microscopy methods, including their principles, configurations, and 3D imaging applications, providing a guideline for biological laboratories with different 3D imaging needs.

scholarly journals Wavelength-Selective Phase-Shifting Digital Holography: Color Three-Dimensional Imaging Ability in Relation to Bit Depth of Wavelength-Multiplexed Holograms

2018 ◽  
Vol 8 (12) ◽  
pp. 2410 ◽  
Author(s):  
Tatsuki Tahara ◽  
Reo Otani ◽  
Yasuhiro Takaki
Keyword(s):  
Digital Holography ◽  
3D Imaging ◽  
Dynamic Range ◽  
Three Dimensional ◽  
Image Sensor ◽  
Phase Shifting ◽  
3D Images ◽  
Wavelength Resolution ◽  
The Relationship

The quality of reconstructed images in relation to the bit depth of holograms formed by wavelength-selective phase-shifting digital holography was investigated. Wavelength-selective phase-shifting digital holography is a technique to obtain multiwavelength three-dimensional (3D) images with a full space-bandwidth product of an image sensor from wavelength-multiplexed phase-shifted holograms and has been proposed since 2013. The bit resolution required to obtain a multiwavelength holographic image was quantitatively and experimentally evaluated, and the relationship between wavelength resolution and dynamic range of an image sensor was numerically simulated. The results indicate that two-bit resolution per wavelength is required to conduct color 3D imaging.

In SituX-Ray Tomography Measurements of Deformation in Cellular Solids

MRS Bulletin ◽  
2003 ◽  
Vol 28 (4) ◽  
pp. 284-289 ◽  
Author(s):  
E. Maire ◽  
A. Elmoutaouakkil ◽  
A. Fazekas ◽  
L. Salvo
Keyword(s):  
3D Imaging ◽  
Recent Literature ◽  
Three Dimensional ◽  
Cellular Solids ◽  
Aluminum Foams ◽  
3D Images ◽  
Closed Cell ◽  
Pertinent Information ◽  
Deformation Modes

AbstractThe use of microtomography to study the structure and especially the deformation modes of cellular solids is reviewed in this article. First, the technique is described in detail. Examples illustrating the power of the coupling ofin situdeformation with three-dimensional (3D) imaging, drawn from the recent literature and the authors' own work, are then given. The most detailed example is the study of the deformation modes of several samples made of different aluminum foams. Four kinds of closed-cell foams were investigated, corresponding to different routes available today for their manufacture. The initial macrostructure was quantified using the 3D images combined with 3D granulometry, allowing retrieval of pertinent information about the cell size and the wall and strut thicknesses. The global behavior exhibited by the foams during thein situcompression experiments was shown to vary from one brand of material to another. Some of these variations can be explained by differences in the known microstructure and the measured macrostructure of the samples.

scholarly journals Application of Three-Dimensional Imaging to the Intestinal Crypt Organoids and Biopsied Intestinal Tissues

2013 ◽  
Vol 2013 ◽  
pp. 1-9 ◽  
Author(s):  
Yun Chen ◽  
Ya-Hui Tsai ◽  
Yuan-An Liu ◽  
Shih-Hua Lee ◽  
Sheng-Hong Tseng ◽  
...  
Keyword(s):  
3D Imaging ◽  
Imaging System ◽  
3D Structure ◽  
Three Dimensional ◽  
Spatial Relationship ◽  
Intestinal Transplantation ◽  
Muscle Layer ◽  
3D Images ◽  
Nerve Network ◽  
Transplant Patients

Two-dimensional (2D) histopathology is the standard analytical method for intestinal biopsied tissues; however, the role of 3-dimensional (3D) imaging system in the analysis of the intestinal tissues is unclear. The 3D structure of the crypt organoids from the intestinal stem cell culture and intestinal tissues from the donors and recipients after intestinal transplantation was observed using a 3D imaging system and compared with 2D histopathology and immunohistochemistry. The crypt organoids and intestinal tissues showed well-defined 3D structures. The 3D images of the intestinal tissues with acute rejection revealed absence of villi and few crypts, which were consistent with the histopathological features. In the intestinal transplant for megacystis microcolon intestinal hypoperistalsis syndrome, the donor’s intestinal tissues had well-developed nerve networks and interstitial cells of Cajal (ICCs) in the muscle layer, while the recipient’s intestinal tissues had distorted nerve network and the ICCs were few and sparsely distributed, relative to those of the donor. The 3D images showed a clear spatial relationship between the microstructures of the small bowel and the features of graft rejection. In conclusion, integration of the 3D imaging and 2D histopathology provided a global view of the intestinal tissues from the transplant patients.

scholarly journals Localization of proteoglycan monomer and link protein in the matrix of bovine articular cartilage: An immunohistochemical study.

1980 ◽  
Vol 28 (7) ◽  
pp. 621-635 ◽  
Author(s):  
A R Poole ◽  
I Pidoux ◽  
A Reiner ◽  
L H Tang ◽  
H Choi ◽  
...  
Keyword(s):  
Articular Cartilage ◽  
Articular Surface ◽  
Guanidine Hydrochloride ◽  
Cartilage Matrix ◽  
Binding Studies ◽  
Superficial Zone ◽  
Surface Culture ◽  
Bovine Articular Cartilage ◽  
Link Protein ◽  
The Matrix

Using monospecific antisera and immunofluorescence microscopy, proteoglycan monomer (PG), and link proteins were demonstrated throughout the extracellular matrix of bovine articular cartilage. A narrow band of strong pericellular staining was usually observed for both molecules, indicating a pericellular concentration of proteoglycan monomer: this conclusion was supported by dye-binding studies. Whereas PG was evenly distributed throughout the remaining matrix, more link protein was detectable in interterritorial sites in middle and deep zones. Well-defined zones of weaker territorial staining for link protein stained strongest for chondroitin sulfate. Trypsin treatment of cartilage resulted in a loss of most of the PG staining, but some selective retention of link protein, particularly around chondrocytes in the superficial zone at and near the articular surface. This residual staining was largely removed if sections were fixed after chondroitinase treatment. After extraction of cartilage with 4M guanidine hydrochloride, only PG remained and this was concentrated in the superficial zone. These observations are shown to support the concept of aggregation of PG and link protein with hyaluronic acid (HA) in cartilage matrix, and the binding of PG and link protein to HA, which is attached to the chondrocyte surface. Culture of cartilage depleted of PG and link protein by trypsin demonstrated that individual chondrocytes can secrete both PG and link proteins and that the organization of cartilage matrix can be regenerated in part over a period of 4 days.

Validation of a Vision-Based, Three-Dimensional Facial Imaging System

2003 ◽  
Vol 40 (5) ◽  
pp. 523-529 ◽  
Author(s):  
A. Ayoub ◽  
A. Garrahy ◽  
C. Hood ◽  
J. White ◽  
M. Bock ◽  
...  
Keyword(s):  
3D Imaging ◽  
Cleft Lip ◽  
Imaging System ◽  
Three Dimensional ◽  
Procrustes Analysis ◽  
Facial Morphology ◽  
3D Images ◽  
Registration Errors ◽  
Measuring Machine ◽  
Full Face

Objective The aim of this study was to assess the accuracy of a newly developed three-dimensional (3D) imaging system in recording facial morphology. Methods Twenty-one infants with cleft lip each had a full-face alginate impression taken at the time of primary lip repair, and a stone cast was constructed from each impression. Five anthropometric points were marked on each cast. Each cast was digitized, and the 3D co-ordinates of the five points were obtained using a co-ordinate measuring machine (CMM, Ferranti) of documented accuracy (9.53 μm). Each cast was scanned in four positions using a computerized stereophotogrammetry (C3D) system. The five points were located on the 3D images, and their 3D co-ordinates were extracted by three operators. The co-ordinate systems produced by C3D were aligned, via translation and rotation, to match the CMM co-ordinate system using partial ordinary procrustes analysis. The displacements of the adjusted C3D co-ordinates from the reference co-ordinates were then measured. Three different types of errors were identified: operator, system, and registration errors. Results Operator error was within 0.2 mm of the true co-ordinates of the landmarks. C3D was accurate within 0.4 mm. The average displacement of points over the 21 casts at four positions for the three operators was 0.79 mm (median 0.68). Conclusions The presented 3D imaging system is reliable in recording facial deformity and could be utilized in recording cleft deformities and measuring the changes following surgery

scholarly journals Three-Dimensional Image Quality Evaluation and Optimization Based on Convolutional Neural Network

2021 ◽  
Vol 38 (4) ◽  
pp. 1041-1049
Author(s):  
Xiujuan Luo
Keyword(s):  
Neural Network ◽  
Image Quality ◽  
Convolutional Neural Network ◽  
3D Imaging ◽  
Quality Evaluation ◽  
Three Dimensional ◽  
3D Image ◽  
3D Images ◽  
Image Quality Evaluation ◽  
Global And Local

Currently, three-dimensional (3D) imaging has been successfully applied in medical health, movie viewing, games, and military. To make 3D images more pleasant to the eyes, the accurate judgement of image quality becomes the key step in content preparation, compression, and transmission in 3D imaging. However, there is not yet a satisfactory evaluation method that objectively assesses the quality of 3D images. To solve the problem, this paper explores the evaluation and optimization of 3D image quality based on convolutional neural network (CNN). Specifically, a 3D image quality evaluation model was constructed, and a 3D image quality evaluation algorithm was proposed based on global and local features. Next, the authors expounded on the preprocessing steps of salient regions in images, depicted the fusion process between global and local quality evaluations, and provided the way to process 3D image samples and acquire contrast-distorted images. The proposed algorithm was proved effective through experiments.

The Strain-Softening of Bovine Articular Cartilage Under Infinitesimal Deformation in Unconfined Compression

2001 ◽  
Author(s):  
Christopher C.-B. Wang ◽  
Nadeen O. Chahine ◽  
Terri-Ann N. Kelly ◽  
W. Michael Lai ◽  
Clark T. Hung ◽  
...  
Keyword(s):  
Articular Cartilage ◽  
Strain Softening ◽  
Articular Surface ◽  
Osmotic Swelling ◽  
Bovine Articular Cartilage ◽  
Softening Mechanism ◽  
Softening Behavior ◽  
Depth Direction ◽  
Swelling Equilibrium ◽  
Triphasic Theory

Abstract Significant strain-softening of articular cartilage along its depth direction has been observed in several studies [1,2], where the free-swelling equilibrium state of the tissue was taken to be the reference configuration for subsequent deformation. Microstructural models have been proposed to interpret this softening as buckling of pre-stressed collagen fibers [3,4]. In this study, we propose that a constitutive model which accounts for the disparity in tensile and compressive moduli of cartilage as well as the osmotic swelling response, can explain this experimentally observed strain-softening mechanism. The strain-softening behavior of the tissue is investigated experimentally and theoretically along three mutually perpendicular directions: directions parallel and perpendicular to the split line direction (1- and 2-direction), and normal to the articular surface (3-direction). A conewise nonlinear elasticity model is incorporated into the triphasic theory [5] to interpret this strain-softening in the context of Donnan osmotic swelling [6].

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