scholarly journals Comparative Clinical Study of Different Multiplex Real Time PCR Strategies for the Simultaneous Differential Diagnosis between Extrapulmonary Tuberculosis and Focal Complications of Brucellosis

2013 ◽  
Vol 7 (12) ◽  
pp. e2593 ◽  
Author(s):  
Rocio Sanjuan-Jimenez ◽  
Pilar Morata ◽  
Pilar Bermúdez ◽  
M. José Bravo ◽  
Juan D. Colmenero
Keyword(s):  
Differential Diagnosis ◽  
Clinical Study ◽  
Real Time ◽  
Real Time Pcr ◽  
Extrapulmonary Tuberculosis ◽  
Comparative Clinical Study

scholarly journals Rapid Differential Diagnosis between Extrapulmonary Tuberculosis and Focal Complications of Brucellosis Using a Multiplex Real-Time PCR Assay

PLoS ONE ◽  
2009 ◽  
Vol 4 (2) ◽  
pp. e4526 ◽  
Author(s):  
María Isabel Queipo-Ortuño ◽  
Juan D. Colmenero ◽  
Pilar Bermudez ◽  
María José Bravo ◽  
Pilar Morata
Keyword(s):  
Differential Diagnosis ◽  
Real Time ◽  
Real Time Pcr ◽  
Extrapulmonary Tuberculosis ◽  
Pcr Assay

scholarly journals Preliminary clinical study using a multiplex real-time PCR test for the detection of bacterial and fungal DNA directly in blood

2010 ◽  
Vol 16 (6) ◽  
pp. 774-779 ◽  
Author(s):  
F. Wallet ◽  
S. Nseir ◽  
L. Baumann ◽  
S. Herwegh ◽  
B. Sendid ◽  
...  
Keyword(s):  
Clinical Study ◽  
Real Time ◽  
Real Time Pcr ◽  
Fungal Dna ◽  
Pcr Test

scholarly journals Evaluation of Real Time PCR for the diagnosis of Extrapulmonary Tuberculosis and comparison with AFB Microscopy among Bangladeshi population

2012 ◽  
Vol 2 (1) ◽  
pp. 26-32 ◽  
Author(s):  
MR Chowdhury ◽  
SMN Bari ◽  
KS Islam ◽  
AGM Rakibuzzaman ◽  
S Afrin
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Extrapulmonary Tuberculosis ◽  
Percutaneous Nephrostomy ◽  
The Body ◽  
Public Health Issue ◽  
Pcr Analysis ◽  
Global Public Health ◽  
Pcr Assay ◽  
Bangladeshi Population

Tuberculosis is a global public health issue and a disease burden in Africa and Asia. Bangladesh is one of the high burden countries in Southeast Asian region. Mycobacterium tuberculosis the causative agent of tuberculosis, is a devastating bacterium because it spreads person to person, acquired multiple antibiotic resistance and most importantly has the limitation in rapid diagnosis, specially in case of extrapulmonary infection. Here we showed Real-Time PCR assay with the primers targeting IS6110 is a better method than conventional AFB microscopy for the diagnosis of extrapulmonary tuberculosis. For the comparative study 99 extrapulmonary specimens from 9 different parts of the body were collected from suspected patients. Among those only 10 samples were positive, where 50% them were scanty positive in AFB microscopy, on the other hand 33 samples were positive in Real–Time PCR assay. All samples positive in AFB microscopy were also positive in Real-Time PCR assay with an additional 23 positive. Higher percentage of positive results were found in Real-Time PCR analysis in all the samples except Percutaneous nephrostomy (PCN) fluid and Pus. The sharp differences in the result indicate the effectiveness and of the Real-Time PCR assay over the conventional AFB microscopy. High precision and accuracy make it better choice as a diagnostic method for the diagnosis of extrapulmonary tuberculosis. DOI: http://dx.doi.org/10.3329/ijns.v2i1.10880 International Journal of Natural Sciences (2012), 2(1): 26-32

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