scholarly journals Coevolution of the Ile1,016 and Cys1,534 Mutations in the Voltage Gated Sodium Channel Gene of Aedes aegypti in Mexico

2015 ◽  
Vol 9 (12) ◽  
pp. e0004263 ◽  
Author(s):  
Farah Z. Vera-Maloof ◽  
Karla Saavedra-Rodriguez ◽  
Armando E. Elizondo-Quiroga ◽  
Saul Lozano-Fuentes ◽  
William C. Black IV
Keyword(s):  
Aedes Aegypti ◽  
Sodium Channel ◽  
Voltage Gated Sodium Channel ◽  
Voltage Gated ◽  
Channel Gene ◽  
Sodium Channel Gene

A novel F1552/C1552 point mutation in the Aedes aegypti voltage-gated sodium channel gene associated with permethrin resistance

2010 ◽  
Vol 96 (3) ◽  
pp. 127-131 ◽  
Author(s):  
Jintana Yanola ◽  
Pradya Somboon ◽  
Catherine Walton ◽  
Woottichai Nachaiwieng ◽  
La-aied Prapanthadara
Keyword(s):  
Aedes Aegypti ◽  
Sodium Channel ◽  
Point Mutation ◽  
Voltage Gated Sodium Channel ◽  
Voltage Gated ◽  
Channel Gene ◽  
Sodium Channel Gene

scholarly journals A mutation in the voltage-gated sodium channel gene associated with pyrethroid resistance in Latin American Aedes aegypti

2007 ◽  
Vol 16 (6) ◽  
pp. 785-798 ◽  
Author(s):  
K. Saavedra-Rodriguez ◽  
L. Urdaneta-Marquez ◽  
S. Rajatileka ◽  
M. Moulton ◽  
A. E. Flores ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Sodium Channel ◽  
Latin American ◽  
Pyrethroid Resistance ◽  
Voltage Gated Sodium Channel ◽  
Voltage Gated ◽  
Channel Gene ◽  
Sodium Channel Gene

scholarly journals Point mutations in domain II of the voltage-gated sodium channel gene in deltamethrin-resistant Aedes aegypti (Diptera: Culicidae)

2010 ◽  
Vol 45 (2) ◽  
pp. 275-282 ◽  
Author(s):  
Raweewan Srisawat ◽  
Narumon Komalamisra ◽  
Yuki Eshita ◽  
Mingqi Zheng ◽  
Katsushige Ono ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Sodium Channel ◽  
Point Mutations ◽  
Voltage Gated Sodium Channel ◽  
Voltage Gated ◽  
Channel Gene ◽  
Sodium Channel Gene

scholarly journals Evidence for gene duplication in the voltage-gated sodium channel gene of Aedes aegypti

2013 ◽  
Vol 2013 (1) ◽  
pp. 148-160 ◽  
Author(s):  
Ademir Jesus Martins ◽  
Luiz Paulo Brito ◽  
Jutta Gerlinde Birggitt Linss ◽  
Gustavo Bueno da Silva Rivas ◽  
Ricardo Machado ◽  
...  
Keyword(s):  
Gene Duplication ◽  
Aedes Aegypti ◽  
Sodium Channel ◽  
Voltage Gated Sodium Channel ◽  
Voltage Gated ◽  
Channel Gene ◽  
Sodium Channel Gene

scholarly journals Detection of Polymorphism on Voltage-gated Sodium Channel Gene of Indonesian Aedes aegypti Associated with Resistance to Pyrethroids

2018 ◽  
Vol 10 (3) ◽  
pp. 250-5 ◽  
Author(s):  
Budi Mulyaningsih ◽  
Sitti Rahmah Umniyati ◽  
Tri Baskoro Tunggul Satoto ◽  
Ernaningsih Ernaningsih ◽  
Dwi Aris Agung Nugrahaningsih
Keyword(s):  
Aedes Aegypti ◽  
Dengue Virus ◽  
Sodium Channel ◽  
Pyrethroid Resistance ◽  
Single Amino Acid ◽  
Voltage Gated Sodium Channel ◽  
Voltage Gated ◽  
Channel Gene ◽  
Sodium Channel Gene ◽  
Yogyakarta City

BACKGROUND: Aedes aegypti is a vector of several pathogens including dengue virus. Vector control is an effective way to break the transmission but unfortunately constant use of insecticides ultimately causes vector resistance. Pyrethroids have been used for about 15 years to combat Ae. aegypti in Yogyakarta Province, Indonesia. Single amino acid substitutions in the voltagegated sodium channel associated with pyrethroid resistance constitute one of the main causative factors of knockdown resistance (kdr). The aim of this study is to detect resistant status to cypermetrine and polymorphism on the voltagegated sodium channel gene of Ae. aegypti from 2 dengue endemic areas in Yogyakarta Province (Yogyakarta city and Sleman district).METHODS: Pyrethroid resistance in Ae. aegypti mosquitoes was detected by using CDC Bottle Bioassay. To detect the polymorphism on the voltage-gated sodium channel gene of Ae. aegypti analyses were conducted by using PCR and direct DNA squencing with primers AaSCF1 and AaSCR4 for S989P, I1011M (or V), L1014F sites, and AaSCF7 and AaSCR7 for the F1534C site.RESULTS: According to bioassay, the results for Ae. aegypti from Yogyakarta city (93% mortality) and Sleman district (88% mortality) suggest the possibility of resistance to cypermethrin. We observed polymorphism on voltagegated sodium channel gene on site F1534C (heterozygous).CONCLUSION: The findings provide early evidence that the use of cypermethrin (pyrethroids) in Yogyakarta city and Sleman district, Yogyakarta Province, Indonesia is reducing its effectiveness to control Ae. aegypti. Recommendations include additional tests for confirmation.KEYWORDS: Aedes aegypti, dengue virus, cypermethrin, Bioassay, Yogyakarta

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