scholarly journals The Perfect Host: A Mouse Host Embryo Facilitating More Efficient Germ Line Transmission of Genetically Modified Embryonic Stem Cells

PLoS ONE ◽  
2013 ◽  
Vol 8 (7) ◽  
pp. e67826 ◽  
Author(s):  
Robert A. Taft ◽  
Benjamin E. Low ◽  
Shannon L. Byers ◽  
Stephen A. Murray ◽  
Peter Kutny ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Germ Line ◽  
Genetically Modified ◽  
Embryonic Stem ◽  
Germ Line Transmission ◽  
Host Embryo

Germ line transmission of an inactive N-myc allele generated by homologous recombination in mouse embryonic stem cells

1990 ◽  
Vol 10 (12) ◽  
pp. 6755-6758
Author(s):  
B R Stanton ◽  
S W Reid ◽  
L F Parada
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Homologous Recombination ◽  
Embryonic Development ◽  
Cell Lines ◽  
Germ Line ◽  
Es Cells ◽  
Embryonic Stem ◽  
Es Cell ◽  
Germ Line Transmission

We have disrupted one allele of the N-myc locus in mouse embryonic stem (ES) cells by using homologous recombination techniques and have obtained germ line transmission of null N-myc ES cell lines with transmission of the null N-myc allele to the offspring. The creation of mice with a deficient N-myc allele will allow the generation of offspring bearing null N-myc alleles in both chromosomes and permit study of the role that this proto-oncogene plays in embryonic development.

scholarly journals Effective Production of Microinjectable Blastocysts for Germ-Line Transmission of Embryonic Stem Cells

2004 ◽  
Vol 53 (5) ◽  
pp. 475-477
Author(s):  
Sun-Uk KIM ◽  
Ying-Hao HAN ◽  
Tae-Hoon LEE ◽  
Byung-Hwa HYUN ◽  
Sang-Ho LEE ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Germ Line ◽  
Embryonic Stem ◽  
Germ Line Transmission

Germ line transmission and expression of a corrected HPRT gene produced by gene targeting in embryonic stem cells

Cell ◽  
1989 ◽  
Vol 56 (2) ◽  
pp. 313-321 ◽  
Author(s):  
Simon Thompson ◽  
Alan R. Clarke ◽  
Angela M. Pow ◽  
Martin L. Hooper ◽  
David W. Melton
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Gene Targeting ◽  
Germ Line ◽  
Embryonic Stem ◽  
Hprt Gene ◽  
Germ Line Transmission

scholarly journals Germ line transmission of an inactive N-myc allele generated by homologous recombination in mouse embryonic stem cells.

1990 ◽  
Vol 10 (12) ◽  
pp. 6755-6758 ◽  
Author(s):  
B R Stanton ◽  
S W Reid ◽  
L F Parada
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Homologous Recombination ◽  
Embryonic Development ◽  
Cell Lines ◽  
Germ Line ◽  
Es Cells ◽  
Embryonic Stem ◽  
Es Cell ◽  
Germ Line Transmission

We have disrupted one allele of the N-myc locus in mouse embryonic stem (ES) cells by using homologous recombination techniques and have obtained germ line transmission of null N-myc ES cell lines with transmission of the null N-myc allele to the offspring. The creation of mice with a deficient N-myc allele will allow the generation of offspring bearing null N-myc alleles in both chromosomes and permit study of the role that this proto-oncogene plays in embryonic development.

Germ-line transmission of a disrupted β2microglobulin gene produced by homologous recombination in embryonic stem cells

Nature ◽  
1989 ◽  
Vol 342 (6248) ◽  
pp. 435-438 ◽  
Author(s):  
Maarten Zijlstra ◽  
En Li ◽  
Fereydoun Sajjadi ◽  
Suresh Subramani ◽  
Rudolf Jaenisch
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Homologous Recombination ◽  
Germ Line ◽  
Embryonic Stem ◽  
Germ Line Transmission

The Use of Genetically Modified Embryonic Stem Cells to Generate Transgenic Mice

2004 ◽  
Vol 40 (3) ◽  
pp. 235-238 ◽  
Author(s):  
M. V. Pryzhkova ◽  
I. R. Zakeeva ◽  
A. V. Kibardin ◽  
G. P. Georgiev ◽  
S. L. Kiselev
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Transgenic Mice ◽  
Genetically Modified ◽  
Embryonic Stem

Attempts towards derivation and establishment of bovine embryonic stem cell-like cultures

2005 ◽  
Vol 17 (2) ◽  
pp. 113 ◽  
Author(s):  
Poul Maddox-Hyttel ◽  
Jakob O. Gjørret
Keyword(s):  
Stem Cells ◽  
Adult Stem Cells ◽  
Germ Line ◽  
Current Knowledge ◽  
Mammalian Species ◽  
Embryonic Stem ◽  
Transgenic Animals ◽  
Great Expectations ◽  
Safety Issues ◽  
Germ Line Transmission

Current knowledge on the biology of mammalian embryonic stem cells (ESC) is stunningly sparse in light of their potential value in studies of development, functional genomics, generation of transgenic animals and human medicine. Despite many efforts to derive ESC from other mammalian species, ESC that retain their capacity for germ line transmission have only been verified in the mouse. However, the criterion of germ line transmission may not need to be fulfilled for exploitation of other abilities of these cells. Promising results with human ESC-like cells and adult stem cells have nourished great expectations for their potential use in regenerative medicine. However, such an application is far from reality and substantial research is required to elucidate aspects of the basic biology of pluripotent cells, as well as safety issues associated with the use of such cells in therapy. In this context, methods for the derivation, propagation and differentiation of ESC-like cultures from domestic animals would be highly desirable as biologically relevant models. Here, we review previously published efforts to establish bovine ESC-like cells and describe a procedure used in attempts to derive similar cells from bovine Day 12 embryos.

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