Association Study between Cervical Lesions and Single or Multiple Vaccine-Target and Non-Vaccine Target Human Papillomavirus (HPV) Types in Women from Northeastern Brazil

AbstractPersistent infection with high-risk human papillomavirus (HPV) is the main cause of cervical cancer and the prevalence of HPV types varies depending on the geographic region. Therefore, this study assessed the prevalence of HPV types in women with cervical lesions from Sergipe state, Northeastern Brazil. A cross-sectional study was conducted in women with cervical lesions from March to December 2014. These lesions were investigated by PCR and HPV types were identified by DNA sequencing. 432 patients were included, of which 337 patients tested positive for HPV. Eighteen different HPV types were detected, and high-risk HPV types were detected in 69.2%. HPV 16 (63.4%) was the most prevalent HPV type found, followed by HPV 66 (4.6%), HPV 18 (1.6%) and HPV 45 (1.4%). These results highlight the importance of the high prevalence of HPV 66, which is a possibly carcinogenic virus type not covered by the available vaccines. The prevalence of HPV 16 was high in the studied population, reaffirming the importance of young vaccination. However, the high prevalence of HPV 66 found in this study shows the importance of monitoring the diversity of HPV types in different populations and geographic regions to better understand the impacts of current HPV vaccines.

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Prevalence of precancerous cervical lesions and high-risk human papillomavirus types in Yaounde, Cameroon

The Journal of Infection in Developing Countries ◽

10.3855/jidc.15218 ◽

2021 ◽

Vol 15 (09) ◽

pp. 1339-1345

Author(s):

Richard Tagne Simo ◽

Arsène G Djoko Nono ◽

Hervet Paulin Fogang Dongmo ◽

Paul F Seke Etet ◽

Bertrand Kiafon Fonyuy ◽

...

Keyword(s):

Human Papillomavirus ◽

High Risk ◽

Precancerous Lesions ◽

Health Centre ◽

Oral Contraceptive Pill ◽

Cervical Lesions ◽

Hpv 16 ◽

Hpv Types ◽

Cytologic Abnormalities ◽

Hpv 18

Introduction: Various Human papillomavirus (HPV) types cause cervical cancer, and represent the primary cause of cancer death in Africa and the second cause of most common cancers in Cameroon. Herein, we determined the prevalence of high-risk HPV types in women and associated cervical cytologic abnormalities in Yaounde, Cameroon. Methodology: A cross-sectional study targeting HPV-positive women aged 20 and over was conducted between March and June 2020 at the Saint Martin de Porres’ Health Centre in Yaounde. HPV tests were performed by PCR for detection of HPVs 16, 18, 33, and 45. The test was performed on 616 women using exfoliated cell specimens; then, we processed on cytological diagnosis with Pap smears on HPV positive specimens. Results: The HPV types tested were detected in 137 participants, of which 38.7% with multiple HPV infections, and the remaining part with single HPV infections of type HPV 16 (28.5%), HPV 18 (17.5%), HPV 33 (10.2%), and HPV 45 (5.1%). Cervical cytologic abnormalities were found in 69.34% of participants including: LSIL (49.63%), HSIL (15.32%), ASC-US (3.66%) and AGC (0.73%). Co-infections with HPV 16 and HPV 18 were significantly associated with HSIL (p = 0.001) lesions, while HPV 45 was more common in participants with normal cytology (p = 0.001). Cervical lesion occurrence was significantly associated with the number of sexual partners (p = 0.02) and history of oral contraceptive pill use (p = 0.001). Conclusions: Our results suggest that HPV 16 and 18 are predominant in Yaounde, and are associated with more severe precancerous lesions.

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Human papillomavirus and abnormal cervical lesions among HIV-infected women in HIV-discordant couples from Kenya

Sexually Transmitted Infections ◽

10.1136/sextrans-2019-054052 ◽

2020 ◽

Vol 96 (6) ◽

pp. 457-463 ◽

Cited By ~ 1

Author(s):

Brandon L Guthrie ◽

Anne F Rositch ◽

Joy Alison Cooper ◽

Carey Farquhar ◽

Rose Bosire ◽

...

Keyword(s):

Human Papillomavirus ◽

Hpv Infection ◽

Cervical Neoplasia ◽

Cervical Cytology ◽

High Grade ◽

Cervical Lesions ◽

Hpv Prevalence ◽

High Grade Lesion ◽

Hpv Types ◽

Hpv Screening

ObjectiveHIV infection increases the risk of high-grade cervical neoplasia and invasive cervical carcinoma. The study addresses the limited data describing human papillomavirus (HPV) infection and cervical neoplasia among HIV-infected women in HIV-discordant relationships in sub-Saharan Africa, which is needed to inform screening strategies.MethodsA cross-sectional study of HIV-infected women with HIV-uninfected partners was conducted to determine the distribution of type-specific HPV infection and cervical cytology. This study was nested in a prospective cohort recruited between September 2007 and December 2009 in Nairobi, Kenya. Cervical cells for HPV DNA testing and conventional cervical cytology were collected. HPV types were detected and genotyped by Roche Linear Array PCR assay.ResultsAmong 283 women, the overall HPV prevalence was 62%, and 132 (47%) had ≥1 high-risk (HR)-HPV genotype. Of 268 women with cervical cytology results, 18 (7%) had high-grade cervical lesions or more severe by cytology, of whom 16 (89%) were HR-HPV-positive compared with 82 (41%) of 199 women with normal cytology (p<0.001). The most common HR-HPV types in women with a high-grade lesion or more severe by cytology were HPV-52 (44%), HPV-31 (22%), HPV-35 (22%), HPV-51 (22%) and HPV-58 (22%). HR-HPV genotypes HPV-16 or HPV-18 were found in 17% of women with high-grade lesions or more severe. HR-HPV screening applied in this population would detect 89% of those with a high-grade lesion or more severe, while 44% of women with normal or low-grade cytology would screen positive.ConclusionHR-HPV prevalence was high in this population of HIV-infected women with an uninfected partner. Choice of screening for all HR genotypes versus a subset of HR genotypes in these HIV-infected women will strongly affect the performance of an HPV screening strategy relative to cytological screening. Regional and subpopulation differences in HR-HPV genotype distributions could affect screening test performance.

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Prevalence of Human Papillomavirus (HPV) infection in women living in remote riverine communities in the Amazonas, Brazil

10.21203/rs.2.12030/v1 ◽

2019 ◽

Author(s):

Marco Antonio Zonta ◽

Karina Bonilha Roque ◽

Yara Juliano ◽

Anne Liljander ◽

Markus Cavalar ◽

...

Keyword(s):

Cervical Cancer ◽

Human Papillomavirus ◽

Hpv Infection ◽

Public Health Services ◽

Cervical Lesions ◽

Transmitted Infection ◽

Sexually Transmitted ◽

Remote Areas ◽

Hpv Types ◽

Crucial Part

Abstract Human papillomavirus (HPV) is the most common sexually transmitted infection worldwide. Since some HPV types are associated with the development of cervical cancer, routine screening for HPV plays a crucial part in cancer prevention programs at global level. In Brazil, the cervical cancer rate is among the highest in the world, in particular in indigenous women living in remote areas with limited access to public health services. The objective of this study was to determine the prevalence of HPV infections and their co-occurrence with cervical lesions in Brazilian women living in isolated riverine communities. Moreover, the association between social environment, sexual activity and prevalence of HPV infection was assessed.

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Current studies on human papillomavirus in Saudi Arabia

The Journal of Infection in Developing Countries ◽

10.3855/jidc.6538 ◽

2015 ◽

Vol 9 (06) ◽

pp. 571-576 ◽

Cited By ~ 9

Author(s):

Fatimah Saeed Alhamlan ◽

Ahmed A Al-Qahtani ◽

Mohammed N Al-Ahdal

Keyword(s):

Cervical Cancer ◽

Saudi Arabia ◽

Human Papillomavirus ◽

High Risk ◽

Hpv Infection ◽

World Health ◽

Cervical Lesions ◽

Hpv Types ◽

Health Organization ◽

Low Prevalence

Human papillomavirus (HPV) infection is a significant etiological factor and an important prognosticator in cervical cancer. Indeed, researchers worldwide have confirmed these roles for high-risk HVPs in over 70% of cervical cancer cases. According to the World Health Organization, approximately 561,200 new cancer cases (5.2% of all new cancers) are attributed to HPV infection. Over 120 types of HPV are classified further as either low-risk HPV (LR-HPV) or high-risk HPV (HR-HPV) based on their oncological potential of transforming cells. The LR-HPV types cause benign hyperproliferative lesions (i.e. genital warts) while the HR-HPV types are strongly associated with premalignant and malignant cervical lesions. Data on the prevalence of HPV, survival of infected patients, and mortality rate are scarce in Saudi Arabia. The unsubstantiated assumption of a low prevalence of HPV in Saudi Arabia has contributed to limiting HPV research in this conservative country. Therefore, the goal of this review is to shed light on the current HPV research being conducted and the prevalence of HPV in Saudi Arabia.

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Analysis on influencing factors of viral load in patients with high-risk human papillomavirus

10.21203/rs.3.rs-26821/v2 ◽

2020 ◽

Author(s):

Xuerong Lu ◽

Tiantian Wang ◽

Youzhong Zhang ◽

Yuzhen Liu

Keyword(s):

Multivariate Analysis ◽

Human Papillomavirus ◽

Viral Load ◽

High Risk ◽

Influencing Factors ◽

Cervical Screening ◽

Cervical Lesion ◽

Cervical Lesions ◽

Hpv Types ◽

Hpv Viral Load

Abstract Background: High-risk human papillomavirus (HR-HPV) load is thought to be influenced by many factors, and relationship between viral load and degree of cervical lesion is controversial. This study was to explore possible influencing factors of HR-HPV viral load in uterine cervix.Methods: 605 women who needed colposcopic evaluation for abnormal cervical screening in Affiliated Hospital of Weifang Medical University, China, between November 2017 and September 2018 were enrolled. Cervical specimens were collected from endo- and ectocervix separately using two different cervical brushes. Hybrid Capture II test was used to measure HR-HPV load. Age, histological severity, number of viral types, area and location of cervical lesions were recorded. The correlation between viral load and influencing factors was analysed using univariate and multivariate analysis.Results: HR-HPV load was positively correlated with age, histological severity, multiple HPV types and area of cervical lesions (P<0.05). Viral load with combination of endo- and ectocervical sampling was significantly higher than simple endocervical sampling (P<0.001). Multivariate analysis showed that age, multiple HPV types and area of cervical lesions were independent factors for HR-HPV load with combination of endo- and ectocervical sampling (P<0.05). However, only age and area of cervical lesions were independent factors for viral load with simple endocervical sampling (P<0.05). No significant association was found between viral load and lesion severity in multivariate analysis (P>0.05).Conclusion: HR-HPV load is influenced by age, histological severity, multiple viral types, area of cervical lesion and sampling methods. Age and area of cervical lesions are independent factors for viral load.

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Analysis of influencing factors of viral load in patients with high-risk human papillomavirus

Virology Journal ◽

10.1186/s12985-020-01474-z ◽

2021 ◽

Vol 18 (1) ◽

Author(s):

Xuerong Lu ◽

Tiantian Wang ◽

Youzhong Zhang ◽

Yuzhen Liu

Keyword(s):

Multivariate Analysis ◽

Human Papillomavirus ◽

Viral Load ◽

High Risk ◽

Influencing Factors ◽

Cervical Screening ◽

Cervical Lesion ◽

Cervical Lesions ◽

Hpv Types ◽

Hpv Viral Load

Abstract Background High-risk human papillomavirus (HR-HPV) load is thought to be influenced by many factors, and the relationship between viral load and the degree of cervical lesion is controversial. This study explored the possible influencing factors of HR-HPV viral load in the uterine cervix. Methods A total of 605 women who needed colposcopic evaluation for abnormal cervical screening at the Affiliated Hospital of Weifang Medical University, China, between November 2017 and September 2018 were enrolled. Cervical specimens were collected from the endo- and ectocervix separately using two different cervical brushes. The hybrid capture II test was used to measure HR-HPV load. Age, histological severity, number of viral types, and area and location of cervical lesions were recorded. The correlations between viral load and influencing factors were analysed using univariate and multivariate analyses. Results HR-HPV load was positively correlated with age, histological severity, multiple HPV types and area of cervical lesions (P < 0.05). Viral load with the combination of endo- and ectocervical sampling was significantly higher than simple endocervical sampling (P < 0.001). Multivariate analysis showed that age, multiple HPV types and area of cervical lesions were independent factors for HR-HPV load with a combination of endo- and ectocervical sampling (P < 0.05). However, only age and area of cervical lesions were independent factors for viral load with simple endocervical sampling (P < 0.05). No significant association was found between viral load and lesion severity in multivariate analysis (P > 0.05). Conclusion HR-HPV load is influenced by age, histological severity, multiple viral types, area of cervical lesion and sampling methods. Age and area of cervical lesions are independent factors for viral load.

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Human papillomavirus–specific viral types are common in Mexican women affected by cervical lesions

International Journal of Gynecological Cancer ◽

10.1136/ijgc-00009577-200605000-00015 ◽

2006 ◽

Vol 16 (3) ◽

pp. 1041-1047

Author(s):

P. Piña-Sánchez ◽

D. M. Hernández-Hernández ◽

R. LÓPEZ-ROMERO ◽

G. VÁZQUEZ-ORTÍZ ◽

C. PÉREZ-PLASENCIA ◽

...

Keyword(s):

Human Papillomavirus ◽

High Risk ◽

Low Grade ◽

Squamous Intraepithelial Lesions ◽

Cervical Lesions ◽

Hpv 16 ◽

Female Population ◽

Hpv Types ◽

Intraepithelial Lesions ◽

Hpv 18

Cervical cancer (CC) is the most common in Mexican female population. The human papillomavirus (HPV) 16 and 18 frequencies in worldwide may be different due to geographical distribution. We analyzed the prevalence of HPV types and determinated their association in cervical lesion in a Mexican population. One hundred fifty-nine normal cervical smears, 95 low-grade squamous intraepithelial lesions (LGSIL), 59 high-grade squamous intraepithelial lesions (HGSIL), and 108 CC samples of the patients were collected. HPV types were determined by sequencing. We detected 11 high-risk types, four low-risk types, three not determinated, and two probably high risk. HPV were present in 12%, 57%, 88%, and 92% from normal, LGSIL, HGSIL, and CC samples, respectively. HPV 16 was the most common in all cervical lesions (71.6% in CC). HPV 58 was present in 18.6% of HGSIL, and the HPV 18 in 4.6% of CC. The 76% of all detected viruses belong to A9 species branch. Control women showed high percentage of HPV high-risk infection, suggesting that this is a high-risk group. High frequency of HPV 16 compared with a low incidence of HPV 18 was observed. HPV 58 is frequently detected in HGSIL but low frequency is found in CC. These findings might be considered for HPV screening.

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Analysis of influencing factors of viral load in patients with high-risk human papillomavirus

10.21203/rs.3.rs-26821/v3 ◽

2020 ◽

Author(s):

Xuerong Lu ◽

Tiantian Wang ◽

Youzhong Zhang ◽

Yuzhen Liu

Keyword(s):

Multivariate Analysis ◽

Human Papillomavirus ◽

Viral Load ◽

High Risk ◽

Influencing Factors ◽

Cervical Screening ◽

Cervical Lesion ◽

Cervical Lesions ◽

Hpv Types ◽

Hpv Viral Load

Abstract Background: High-risk human papillomavirus (HR-HPV) load is thought to be influenced by many factors, and the relationship between viral load and the degree of cervical lesion is controversial. This study explored the possible influencing factors of HR-HPV viral load in the uterine cervix. Methods: A total of 605 women who needed colposcopic evaluation for abnormal cervical screening at the Affiliated Hospital of Weifang Medical University, China, between November 2017 and September 2018 were enrolled. Cervical specimens were collected from the endo- and ectocervix separately using two different cervical brushes. The hybrid capture II test was used to measure HR-HPV load. Age, histological severity, number of viral types, and area and location of cervical lesions were recorded. The correlations between viral load and influencing factors were analysed using univariate and multivariate analyses. Results: HR-HPV load was positively correlated with age, histological severity, multiple HPV types and area of cervical lesions (P<0.05). Viral load with the combination of endo- and ectocervical sampling was significantly higher than simple endocervical sampling (P<0.001). Multivariate analysis showed that age, multiple HPV types and area of cervical lesions were independent factors for HR-HPV load with a combination of endo- and ectocervical sampling (P<0.05). However, only age and area of cervical lesions were independent factors for viral load with simple endocervical sampling (P<0.05). No significant association was found between viral load and lesion severity in multivariate analysis (P>0.05). Conclusion: HR-HPV load is influenced by age, histological severity, multiple viral types, area of cervical lesion and sampling methods. Age and area of cervical lesions are independent factors for viral load.

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Prevalence of High-Risk Human Papillomavirus Types Among Women Screened for Cervical Cancer in Yazd, Iran, and Comparison of Cytology, Histology, and Colposcopy Results

Jundishapur Journal of Microbiology ◽

10.5812/jjm.100573 ◽

2020 ◽

Vol 13 (9) ◽

Author(s):

Mojgan Karimi-Zarchi ◽

Nastaran Hajimaghsoudi ◽

Afsarosadat Tabatabai ◽

Mansour Moghimi ◽

Mohammad Shayestehpour ◽

...

Keyword(s):

Cervical Cancer ◽

Human Papillomavirus ◽

High Risk ◽

Pap Smear ◽

Blot Hybridization ◽

High Grade ◽

Cervical Lesions ◽

Hpv Typing ◽

Hpv Types ◽

High Risk Hpv

Background: Human Papillomavirus (HPV) is a DNA virus with more than 100 genotypes, at least 12 of which are high-risk and associated with high-grade cervical lesions. Data on the prevalence of high-risk HPV genotypes among women are not yet available for the total regions of Iran. Objectives: The present study aimed to determine the prevalence of high-risk HPV types among women screened for cervical carcinoma in Yazd and compare the cytology, histology, and colposcopy results. Methods: In this cross-sectional study, 402 women referring to gynecology clinics of Shahid Sadoughi University of Medical Sciences, Yazd, Iran, were selected. The Pap smear and HPV typing were performed on cervical samples. The high-risk HPV types were detected by the polymerase chain reaction (PCR)-based reverse blot hybridization assay. Colposcopy was carried out on patients with high-risk HPV types, and biopsies were taken for histological examination. Results: Among 402 women screened by HPV-PCR, 32 (7.97%) women were positive for high-risk HPV types. Human papillomavirus 16 and HPV18 were the most frequent genotypes (46.9%). The cytology, histology, and colposcopy results were abnormal in 56.2%, 29.1%, and 71.9% of patients, respectively. Pap smear had 100% sensitivity and 58.3% specificity for the detection of high-grade cervical lesions, while these values for colposcopy were 75% and 87.5%, respectively. Conclusions: The frequency of high-risk HPV types was relatively low among women living in Yazd than in those from other provinces of Iran. A significant percentage of patients with HPV had normal cervical cytology and histology. Therefore, HPV typing is recommended to decrease the development of cervical cancer. Colposcopy had acceptable sensitivity and specificity for the detection of high-grade cervical lesions.

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