scholarly journals Predicted strain coverage of a meningococcal multicomponent vaccine (4CMenB) in Portugal

PLoS ONE ◽  
2017 ◽  
Vol 12 (5) ◽  
pp. e0176177 ◽  
Author(s):  
Maria João Simões ◽  
Célia Bettencourt ◽  
Rosita De Paola ◽  
Maria Giuliani ◽  
Mariagrazia Pizza ◽  
...  
Keyword(s):  
Multicomponent Vaccine

scholarly journals Localization within a Proline-Rich Antigen (Ag2/PRA) of Protective Antigenicity against Infection with Coccidioides immitis in Mice

2002 ◽  
Vol 70 (7) ◽  
pp. 3330-3335 ◽  
Author(s):  
Tao Peng ◽  
Lisa Shubitz ◽  
Julie Simons ◽  
Robert Perrill ◽  
Kris I. Orsborn ◽  
...  
Keyword(s):  
Humoral Response ◽  
Optimal Dose ◽  
Amino Acid Sequences ◽  
Full Length ◽  
Foreign Protein ◽  
Recombinant Vaccines ◽  
Coccidioides Immitis ◽  
Plasmid Vaccine ◽  
Multicomponent Vaccine ◽  
Total Immunoglobulin

ABSTRACT Subunits of a proline-rich coccidioidal antigen (Ag2/PRA) of Coccidioides immitis were analyzed by comparison as vaccines in mice. The optimal dose of plasmid vaccine encoding full-length Ag2/PRA was determined to be between 10 and 100 μg. Mice vaccinated with plasmids encoding amino acids (aa) 1 to 106 were as protective as full-length Ag2/PRA (aa 1 to 194). The subunit from aa 27 to 106 was significantly but less protective. Plasmids encoding aa 90 to 151 or aa 90 to 194 were not protective. Analogous results were obtained with recombinant vaccines of the same amino acid sequences. In addition, mixtures of aa 90 to 194 with either aa 1 to 106 or aa 27 to 106 did not enhance protection compared to the active single-recombinant subunits alone. Humoral response of total immunoglobulin G (IgG) and subclasses IgG1 and IgG2a were detectable in subunit vaccinations but at significantly (100-fold) lower concentrations than after vaccination with plasmids encoding full-length Ag2/PRA. Since virtually all protection by vaccination with full-length Ag2/PRA can be accounted for in the first half of the protein (aa 1 to 106), this subunit could make a multicomponent vaccine more feasible by reducing the quantity of protein per dose and the possibility of an untoward reactions to a foreign protein.

scholarly journals Development of a multicomponent vaccine for Streptococcus pyogenes based on the antigenic targets of IVIG

2016 ◽  
Vol 72 (4) ◽  
pp. 450-459 ◽  
Author(s):  
Mark Reglinski ◽  
Nicola N. Lynskey ◽  
Yoon Jung Choi ◽  
Robert J. Edwards ◽  
Shiranee Sriskandan
Keyword(s):  
Streptococcus Pyogenes ◽  
Antigenic Targets ◽  
Multicomponent Vaccine

Transplastomic plants yield a multicomponent vaccine against cysticercosis

2018 ◽  
Vol 266 ◽  
pp. 124-132 ◽  
Author(s):  
Sergio Rosales-Mendoza ◽  
Elizabeth Monreal-Escalante ◽  
Omar González-Ortega ◽  
Marisela Hernández ◽  
Gladis Fragoso ◽  
...  
Keyword(s):  
Transplastomic Plants ◽  
Multicomponent Vaccine

scholarly journals Immunological fingerprint of 4CMenB recombinant antigens via protein microarray reveals key immunosignatures correlating with bactericidal activity

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
E. Bartolini ◽  
E. Borgogni ◽  
M. Bruttini ◽  
A. Muzzi ◽  
M. Giuliani ◽  
...  
Keyword(s):  
Immune Response ◽  
Epitope Mapping ◽  
Ad Hoc ◽  
Protein Microarray ◽  
Age Groups ◽  
Antibody Repertoire ◽  
Human Antibody ◽  
Adolescents And Adults ◽  
High Flexibility ◽  
Multicomponent Vaccine

Abstract Serogroup B meningococcus (MenB) is a leading cause of meningitis and sepsis across the world and vaccination is the most effective way to protect against this disease. 4CMenB is a multi-component vaccine against MenB, which is now licensed for use in subjects >2 months of age in several countries. In this study, we describe the development and use of an ad hoc protein microarray to study the immune response induced by the three major 4CMenB antigenic components (fHbp, NHBA and NadA) in individual sera from vaccinated infants, adolescents and adults. The resulting 4CMenB protein antigen fingerprinting allowed the identification of specific human antibody repertoire correlating with the bactericidal response elicited in each subject. This work represents an example of epitope mapping of the immune response induced by a multicomponent vaccine in different age groups with the identification of protective signatures. It shows the high flexibility of this microarray based methodology in terms of high-throughput information and minimal volume of biological samples needed.

scholarly journals A Multicomponent Vaccine Provides Immunity against Local and Systemic Infections by Group A Streptococcus across Serotypes

mBio ◽  
2019 ◽  
Vol 10 (6) ◽  
Author(s):  
Shuai Bi ◽  
Meiyi Xu ◽  
Ya Zhou ◽  
Xinxin Xing ◽  
Adong Shen ◽  
...  
Keyword(s):  
Virulence Factors ◽  
Vaccine Development ◽  
Group A Streptococcus ◽  
Clinical Manifestations ◽  
Enzyme Linked Immunosorbent Assay ◽  
Th17 Response ◽  
Different Types ◽  
Group A ◽  
Large Repertoire ◽  
Multicomponent Vaccine

ABSTRACT Group A streptococcus (GAS) species are responsible for a broad spectrum of human diseases, ranging from superficial to invasive infections, and are associated with autoimmune disorders. There is no commercial vaccine against GAS. The clinical manifestations of GAS infection may be attributable to the large repertoire of virulence factors used selectively in different types of GAS disease. Here, we selected five molecules, highly conserved among GAS serotypes, and involved in different pathogenic mechanisms, as a multicomponent vaccine, 5CP. Intranasal (i.n.) immunization with 5CP protected mice against both mucosal and systemic GAS infection across serotypes; the protection lasted at least 6 months. Immunization of mice with 5CP constrained skin lesion development and accelerated lesion recovery. Flow cytometry and enzyme-linked immunosorbent assay analyses revealed that 5CP induced Th17 and antibody responses locally and systemically; however, the Th17 response induced by 5CP resolved more quickly than that to GAS when challenge bacteria were cleared, suggesting that 5CP is less likely to cause autoimmune responses. These findings support that immunization through the i.n. route targeting multiple nonredundant virulence factors can induce immunity against different types of GAS disease and represents an alternative strategy for GAS vaccine development, with favorable efficacy, coverage, duration, and safety. IMPORTANCE GAS is among the most common human pathogens and causes a wide variety of diseases, likely more than any other microorganism. The diverse clinical manifestations of GAS may be attributable to its large repertoire of virulence factors that are selectively and synergistically involved in streptococcal pathogenesis. To date, GAS vaccines have not been successful due to multiple serotypes and postinfection sequelae associated with autoimmunity. In this study, five conserved virulence factors that are involved in GAS pathogenesis were used as a combined vaccine. Intranasal immunization with this vaccine induced humoral and cellular immune responses across GAS serotypes and protected against mucosal, systemic, and skin infections. The significance of this work is to demonstrate that the efficacy of GAS vaccines can be achieved by including multiple nonredundant critical virulence factors and inducing local and systemic immunity. The strategy also provides valuable insights for vaccine development against other pathogens.

Predicted strain coverage of a meningococcal multicomponent vaccine (4CMenB) in Europe: a qualitative and quantitative assessment

2013 ◽  
Vol 13 (5) ◽  
pp. 416-425 ◽  
Author(s):  
Ulrich Vogel ◽  
Muhamed-Kheir Taha ◽  
Julio A Vazquez ◽  
Jamie Findlow ◽  
Heike Claus ◽  
...  
Keyword(s):  
Quantitative Assessment ◽  
Qualitative And Quantitative ◽  
Multicomponent Vaccine

scholarly journals Meningococcal Antigen Typing System Development and Application to the Evaluation of Effectiveness of Meningococcal B Vaccine and Possible Use for Other Purposes

2015 ◽  
Vol 2015 ◽  
pp. 1-9 ◽  
Author(s):  
Alexander Domnich ◽  
Roberto Gasparini ◽  
Daniela Amicizia ◽  
Giuseppe Boccadifuoco ◽  
Marzia Monica Giuliani ◽  
...  
Keyword(s):  
System Development ◽  
Specific Antigen ◽  
Surface Antigens ◽  
Cross Reactivity ◽  
Field Application ◽  
Resource Saving ◽  
Qualitative And Quantitative ◽  
Laboratory Techniques ◽  
Typing System ◽  
Multicomponent Vaccine

Development of the 4-component meningococcal serogroup B vaccine (4CMenB) has required new assays for the reliable evaluation of the expression and cross-reactivity of those specific antigen variants that are predicted to be targeted by bactericidal antibodies elicited by the vaccine in different isolates. Existing laboratory techniques, such as multilocus sequence typing, are poorly suited to this purpose, since they do not provide information on the contribution of single vaccine components and therefore cannot be applied to estimate the potential coverage of the multicomponent vaccine. The hSBA, the only correlate of protection against invasive meningococcal disease accepted thus far, cannot conveniently be used to test large number of strains. To overcome these issues, the meningococcal antigen typing system (MATS) has been specifically developed in order to predict 4CMenB coverage of individual meningococcus serogroup B strains. To date, MATS has proved advantageous for several reasons, including its ability to assess both qualitative and quantitative aspects of surface antigens of single strains in a highly reproducible, rapid, and resource-saving manner, while its shortcomings include a possible underestimation of 4CMenB coverage and the use of pooled sera to calculate the positive bactericidal threshold. This paper provides an overview of MATS development and its field application.

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