scholarly journals Catechin Contents, Antioxidant and Antibacterial Activities of Different Types of Indonesian Tea (Camellia sinensis)

2021 ◽  
Vol 24 (2) ◽  
pp. 106
Author(s):  
Isa Nuryana ◽  
Shanti Ratnakomala ◽  
Fahrurrozi Fahrurrozi ◽  
Ario Betha Juanssilfero ◽  
Ade Andriani ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Green Tea ◽  
Camellia Sinensis ◽  
High Performance ◽  
Micrococcus Luteus ◽  
Antibacterial Properties ◽  
Black Tea ◽  
Antibacterial Activities ◽  
Bacterial Strains ◽  
White Tea

Tea is one of the most popular beverages in the world. Produced from Camellia sinensis leaves, tea has been studied to provide health benefits due to the content of important metabolites. This study aimed to investigate the catechin contents, antioxidant, and antibacterial activities of Indonesian tea varieties, namely green tea, black tea, and white tea. Tea infusion was prepared by extracting 1 g of each sample into 10 mL of distilled water and incubated at 80 °C for 60 min. The catechin and epigallocatechin gallate (EGCG) contents of tea extracts were determined using high-performance liquid chromatography (HPLC). Antioxidant activity was measured using the free radical method with 2,2-diphenyl-1-pycrylhidrazyl (DPPH), while antimicrobial activity was assesed using paper disc diffusion assay. The results indicated that green tea had the highest contents of catechin (646 ± 17.14 mg/L) and EGCG (997.8 ± 36.72 mg/L), and antioxidant activity with IC50 of  5.65 µg/mL. Furthermore, green tea and white tea extracts showed inhibitory activity against Gram-positive bacteria such as Micrococcus luteus, Bacillus subtilis, and Staphylococcus aureus whereas black tea had no activity against all bacterial strains tested. Generally, we concluded that white tea and green tea contributed to the higher content of catechins and exhibited strong antioxidant and antibacterial properties.

scholarly journals CONCENTRAÇÃO DE CAFEÍNA EM Camellia sinensis

2018 ◽  
Vol 10 (3) ◽  
pp. 09-15
Author(s):  
Daniele Nascimento Silva ◽  
Marielle Flaviane de Carvalho Pimentel ◽  
Daniel Ângelo Macena ◽  
Vinícius Marques Gomes
Keyword(s):  
Green Tea ◽  
Camellia Sinensis ◽  
Black Tea ◽  
Ultraviolet Region ◽  
Physiological Effects ◽  
Chemical Dependence ◽  
White Tea ◽  
Low Concentrations ◽  
The World

Considering that tea is the second most consumed drink in the world it is important not to have excess caffeine in its composition since it causes some physiological effects to humanhealth such as diuretic and chemical dependence. The present project had as objective to compare the results obtained from the determination of caffeine in teas from the Camellia sinensis species with those found in the literature. For that, three samplesof white tea, three of green tea and three of black tea of the same brand were used. The analyzes were performed in triplicates and caffeine determined by spectrophotometry in the ultraviolet region. Black tea had the highest caffeine content (0.106 mg / g), white tea 0.082 mg / g and green tea the lowest (0.067 mg / g). However, the three types of teas have low concentrations when compared to the literature that presented values between 14.3 and 40.0 mg / g of caffeine of tea.

A Comparative Study of Antioxidant Potential and Phenolic Content in White (Silver Needle), Green and Black Tea

2019 ◽  
Vol 15 (4) ◽  
pp. 415-420 ◽  
Author(s):  
Amandeep Kaur ◽  
Sumaya Farooq ◽  
Amit Sehgal
Keyword(s):  
Antioxidant Activity ◽  
Green Tea ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Radical Scavenging ◽  
Black Tea ◽  
Total Phenolic ◽  
White Tea ◽  
Different Types ◽  
Different Parts

Background: White, green and black are the major tea types obtained from the same tea plant (Camellia sinensis). The differences in processing result in different types of tea, of which green tea (20%) and black tea (78%) are the most consumed worldwide and also white tea is gaining popularity due to its subtle taste and recent evidences about its health benefits. Silver needle is a sub type of white tea, made purely from buds whereas green tea is procured from leaves that are steamed or panfried, and to produce black tea, the tea leaves are withered, fully oxidized and then dried. Objective: The present study was undertaken to analyze and compare the antioxidant activity and phenolic composition of white (silver needle), green and black teas of same brand. Method: The radical scavenging ability of different tea types was measured using various antioxidant assays. The total phenolic content was also estimated using Folin-Ciocalteu’s method. Results: The white tea (silver needle) demonstrated highest radical scavenging activity followed by green and then black tea in various antioxidant assays performed. The total phenolic content of different types of tea exhibited the following order: white tea (133.30mg/g) > green tea (118.37mg/g) > black tea (101.8mg/g). A strong correlation was observed between the total phenolic content and antioxidant activity of different tea types, the correlation coefficient ranged from 0.87-0.97. Conclusion: White tea (silver needle) manifested highest antioxidant activity followed by green and least in black tea. The pronounced antioxidant ability of WT (silver needle) may be ascribed to the different parts of the plant (unopened buds) and the least processing it undergoes, followed by obtaining green tea and black tea from different parts of the plant (leaves), and variation in degree of processing.

scholarly journals Optimization of HIB Value Combination of Tween 60 and Span 80 on Cream Formulation of Ethanol Extract of Green Tea Leaves (Camellia Sinensis L.)

2018 ◽  
Vol 23 (3) ◽  
pp. 124
Author(s):  
Fransiska Lisa Anindya Putri ◽  
Akhmad Kharis Nugroho ◽  
Erna Prawita Setyowati
Keyword(s):  
Antioxidant Activity ◽  
Green Tea ◽  
Camellia Sinensis ◽  
Ethanol Extract ◽  
Tea Leaves ◽  
Lattice Design ◽  
Dpph Method ◽  
Green Tea Leaves ◽  
Span 80 ◽  
Tween 60

Green tea (Camellia sinensis L.) is known to have ability to protect skin against free radicals. This is supported by polyphenol compound catechin. This research aims to determine the optimum Hydrophilic-Lipophilic Balance (HLB) value of Tween 60 and Span 80 compositions on the optimum cream formula of ethanol extract of green tea leaves. Tea leaves are extracted by macerating using 70% ethanol. Catechin in extract is known from Thin Layer Chromatography (TLC) test with silica gel 60 F254 as stationary phase and ethyl acetate:aquadest:formic acid (18:1:1 v/v) as mobile phase. Antioxidant activity is determined by 2,2-Diphenyl-1-picryhydrazyl (DPPH) method and value of Inhibition Concentration 50% (IC50) is then calculated. Formula optimization using Design Expert® version 7.1.5 (DX 7) software, Simplex Lattice Design (SLD) method with two components Tween 60 and Span 80. Cream is characterized according to physical properties organoleptic, homogeneity, viscosity, pH, spreadability, adhesiveness, and cream type. The optimum formula obtained is then tested for physical stability for 4 weeks at room temperature (28±2°C) and data are statistically analyzed using one-way ANOVA. The extract contains catechin proved with Retention factor (Rf) value 0.8 and has antioxidant activity with IC50 value 56.35 ppm. 6.4% Tween 60 and 3.6% Span 80 result an optimum HLB value 11.1. It has viscosity 2897.50±35.94 mPa.s, spreadability 18.44±0.06 cm2, adhesiveness 0.85±0.05 seconds, and pH 4.530±0.002. Statistical test shows that the cream is significantly altered at pH, but does not significantly change in viscosity, spreadability, and adhesiveness after being stored for 4 weeks.

scholarly journals Phytochemicals and Quality of Green and Black Teas from Different Clones of Tea Plant

2020 ◽  
Vol 2020 ◽  
pp. 1-13
Author(s):  
Yadav KC ◽  
Ashok Parajuli ◽  
Bishnu Bahadur Khatri ◽  
Lila Devi Shiwakoti
Keyword(s):  
Antioxidant Activity ◽  
Green Tea ◽  
Antioxidant Properties ◽  
Water Extract ◽  
Black Tea ◽  
Tea Plant ◽  
Total Quality ◽  
Dry Extract ◽  
Caffeine Content ◽  
Tea Plants

Tea is a popular drink with refreshing and functional properties. Bud, 1st leaf, and 2nd leaf of five varieties of tea clones (Gumti Takda-78, Ambari, Chiniya, and Tinali, which are popular in tea plantation area of Nepal) were collected and used for preparing green and orthodox black tea to study antioxidant activity, phytochemicals profile, chemical content, and sensory parameters. One or two leaves were hand-plucked from each bush to get a sample of about 100 leaves and processed for green and orthodox black tea for different clones of tea plants. Phytochemicals, antioxidant activity, and caffeine content were found higher in bud followed by 1st leaf and 2nd leaf for all clones of tea plants. Both types of tea (green and black) from Gumti were significantly (p<0.05) higher having tannin content, flavonoid content, total polyphenol content, caffeine content, and IC50 value of 49.15 and 36.23 (mg GAE/g dry extract), 358.9 and 350.4 (mg QE/g dry extract), 590.5 and 570 (mg GAE/g dry extract), 2.85 and 2.94%, and 45.15 & 51.88 μg/mL, respectively. Green and orthodox tea from Takda-78 was found higher in caffeine content and the least in Tinali for both types of tea. Moisture, water extract, total ash, acid-insoluble ash, and crude fiber content in tea (green and black) from Gumti were found to be 5.4% & 5.37%, 65.89% & 71.46%, 5.524% & 6.52%, 0.46% & 0.57%, and 7.96% & 10.27%, respectively. The ratio of theaflavin and thearubigin (TF : TR) was found 1 : 8.61, 1 : 9.36, 1 : 9.70, 1 : 12.87, and 1 : 6.36 in Takda-78, Ambari, Gumti, Chiniya, and Tinali respectively. The total quality score in green tea (85.13%) and black tea (85.78%) from Gumti was significantly higher than others. Phytochemicals and antioxidant properties of green tea were significantly (p<0.05) higher than those of orthodox black tea for all clones of tea plant. This study suggests Gumti variety to be used in green and orthodox black tea processing for higher phytochemical, chemical, sensory quality, and antioxidant activity.

scholarly journals Antioxidant and antifungal activities of Camellia sinensis (L.) Kuntze leaves obtained by different forms of production

2016 ◽  
Vol 76 (2) ◽  
pp. 428-434 ◽  
Author(s):  
L. E. A. Camargo ◽  
L. S. Pedroso ◽  
S. C. Vendrame ◽  
R. M. Mainardes ◽  
N. M. Khalil
Keyword(s):  
Antioxidant Activity ◽  
Camellia Sinensis ◽  
Hypochlorous Acid ◽  
Black Tea ◽  
Total Phenolic ◽  
Myeloperoxidase Activity ◽  
Conjugated Diene ◽  
Candida Spp ◽  
Anticandidal Activity

Abstract The antioxidant and anticandidal activities of leaves obtained from Camellia sinensis by non-fermentation (green and white teas), semi-fermentation (red tea) and fermentation method (black tea) were investigated. It was evaluated the total phenolic content by Folin-Ciocalteau assay; antioxidant capacities were evaluated in vitro using DPPH and ABTS radicals, hypochlorous acid and superoxide anion scavenger assays, induced hemolysis, lipid peroxidation by conjugated diene formation and myeloperoxidase activity. Anticandidal activity was performed on three strains of Candida spp. The results showed that non-fermented teas have a higher concentration of phenolic compounds, and then presented the best inhibitory activity of AAPH-induced hemolysis, the best inhibition of conjugated diene formation and more pronounced antioxidant activity in all tests. The highest anticandidal activity was obtained from fermented tea, followed by non-fermented tea. These results indicate that the antioxidant activity demonstrated has no direct relation with the anticandidal activity.

Determination of lemon and carbonate effects on caffeine content of various teas and investigation of daily caffeine intakes / Çeşitli çayların içerdikleri kafein miktarlarına limon ve karbonatın etkisi ve günlük alım miktarlarının incelenmesi

2016 ◽  
Vol 41 (5) ◽  
Author(s):  
Funda Demir ◽  
Azmi Seyhun Kıpçak ◽  
Özgül Dere Özdemir ◽  
Mehmet Burçin Pişkin ◽  
Emek Möröydor Derun
Keyword(s):  
Green Tea ◽  
Camellia Sinensis ◽  
Black Tea ◽  
Daily Maximum ◽  
Caffeine Content ◽  
Tea Infusions ◽  
Health Field

AbstractObjective: Tea (Camellia sinensis), has been used for health field in thousands of years. Caffeine is one of the key component in tea and investigation of caffeine is a popular working subject among the researches. The novelty of this study is not only the determination of the caffeine contents of the teas, but also how addition of lemon and carbonate effects the caffeine contents. Another aim of the study is the investigation of the daily caffeine intakes from teas.Methods: Tea infusions were prepared and caffeine contents were extracted by using chloroform and determined by UV-Vis Spectrophotometer. For lemon and carbonate addition experiments, lemon was added before the caffeine analysis and carbonate was added at the beginning.Results: Maximum caffeine contents from highest to lowest were seen in black, earl grey and green in classic teas; fennel, mint and sage in herbal teas; lemon, apple and rosehip in fruit teas. With lemon addition caffeine contents were increased except green tea and with carbonate addition caffeine contents were decreased except black and fennel tea. Daily caffeine intakes are found between 32.10% (green tea with carbonate) - 77.20% (black tea with lemon), 1.85% (sage tea with carbonate) - 4.05% (fennel tea with lemon) and 2.10% (rosehip with carbonate) - 4.00% (lemon tea with lemon) in classic, herbal and fruit teas, respectively.Conclusion: The significance of this study indicates that herbal and fruit teas contain caffeine, which is assumed zero in literature. The caffeine amount of herbal teas (20.79±0.36-30.68±0.63 ppm) were found barely higher than the fruit teas (22.87±0.54-28.54±0.75 ppm) but daily maximum caffeine intakes were found less than 5%. The daily maximum caffeine intakes were found in the teas as 525.36±2.84-20.79±0.36 ppm, where lemon addition increased to 617.90±3.54-22.97±0.58 ppm and carbonate addition decreased to 488.54±2.05-16.84±0.28 ppm.

scholarly journals Perbandingan Total Mikroba Kombucha dengan Variasi Jenis Teh dan Lama Fermentasi

bionature ◽  
2020 ◽  
Vol 21 (2) ◽  
Author(s):  
Azrini Khaerah ◽  
Halijah Halijah ◽  
Nurhilmi Nawir
Keyword(s):  
Green Tea ◽  
Fermentation Process ◽  
Black Tea ◽  
Total Cell ◽  
White Tea ◽  
Oolong Tea ◽  
Fermented Beverage ◽  
Fermentation Period

Abstract. Kombucha is a fermented beverage made from tea and sugar. This fermentation is a work of bacteria and yeast consorsia that make a powerful symbiosis cooperation resulting kombucha with quality chemically increased. This kombucha is a healthful beverage for its consumer. Fermentation process can change the tea phisically, such as its taste, colour and flavor. During the fermentation period, the total cell of bacteria and yeast also change. In this research, kombucha is made from 4 different tea, such as black tea, green tea, oolong tea and white tea. As a result, found that the tea as a substrate and also the fermentation period affect the total of colony and cell of bacteria and yeast. In white tea and black tea kombucha perform decreased of total cell bacteria and yeast by the fermentation period, while in green tea and oolong tea kombucha increased. Keywords. kombucha, fermentation, total microbes, SCOBY, haemacytometer

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