scholarly journals Variation in Amino Acids Composition through Pre column Derivatisation using Phenylisothiocyanate by HPLC in Some Economically Important Less Explored Wild Allium Species of Western Himalayas

2020 ◽  
Vol 5 (3) ◽  
pp. 185-191
Author(s):  
H. K. Pandey ◽  
Anchala Guglani ◽  
G. C. Kharakwal ◽  
G. Balakrishna ◽  
Madhu Bala
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
High Performance ◽  
Gradient Elution ◽  
Essential Amino Acids ◽  
Western Himalayas ◽  
Allium Species ◽  
Aqueous Buffer ◽  
Acid Hydrolysate ◽  
Amino Acids Composition

Less explored Allium species are being used as green vegetables and as a condiment by the local inhabitant of high-altitude areas of Uttarakhand. In the present study, four economically important less explored wild Allium species viz. A. auriculatum, A. ampeloprasum, A. ascalonicum and A. rubellum have screened for their amino acid contents by High-performance liquid chromatography. The hydrochloric acid hydrolysate of these four species, the Allium amino acids were derivatised with phenylisothiocyanate resulting phenylthiocarbamyl derivatives and separated on a reverse-phase column by gradient elution with aqueous buffer and acetonitrile-water (60:40 v/v) and detected in UV region at 254 nm. The Pico-tag (3.9 × 300 mm) C18 column equilibrated with the solvents. The elution of all amino acid derivatives was achieved in 12 min using gradient elution by increasing concentration of aqueous buffer and acetonitrile-water. Total seventeen amino acids were present in these Allium species. The ratio of essential amino acids to total amino acids found 1:2.14 in Allium auriculatum, 1:2.35 in Allium ampeloprasum, 1:1.38 in A. ascalonicum and 1:3.44 in Allium rubellum. These less explored Allium species contained substantial amount of essential and non-essential amino acids. Among these Allium species, Allium auriculatum and Allium rubellum found most promising as far as essential and non-essential amino acids composition concerned.

scholarly journals Quality of Soybean Products in Terms of Essential Amino Acids Composition

Molecules ◽  
2021 ◽  
Vol 26 (16) ◽  
pp. 5071
Author(s):  
Wanda Kudełka ◽  
Małgorzata Kowalska ◽  
Marzena Popis
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Moisture Content ◽  
Genetically Modified ◽  
Essential Amino Acids ◽  
Raw Material ◽  
Quantitative Composition ◽  
Genetically Modified Soybean ◽  
Amino Acids Composition ◽  
Complete Protein

The content of protein, moisture content and essential amino acids in conventional and genetically modified soybean grain and selected soybean products (soybean pâté, soybean drink, soybean dessert, tofu) was analyzed in this paper. The following comparative analysis of these products has not yet been carried out. No differences were observed in the amino acid profiles of soybeans and soybean products. The presence of essential amino acids was confirmed except for tryptophan. Its absence, however, may be due not to its absence in the raw material, but to its decomposition as a result of the acid hydrolysis of the sample occurring during its preparation for amino acid determination. Regardless of the type of soybean grain, the content of protein, moisture content and essential amino acids was similar (statistically insignificant difference). Thus, the type of raw material did not determine these parameters. There was a significant imbalance in the quantitative composition of essential amino acids in individual soybean products. Only statistically significant variation was found in genetically modified and conventional soybean pâté. Moreover, in each soy product their amount was lower irrespective of the raw material from which they were manufactured. Therefore, the authors indicate the necessity of enriching soybean products with complete protein to increase their nutritional value.

scholarly journals Analisis Profil Protein dan Asam Amino Sarang Burung Walet (Collocalia fuchiphaga)Asal Painan

2014 ◽  
Vol 4 (1) ◽  
Author(s):  
Lina Elfita
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Aspartic Acid ◽  
High Performance ◽  
Hplc Analysis ◽  
Protein Profile ◽  
Essential Amino Acids ◽  
Bird Nest ◽  
Sds Page ◽  
West Sumatra

Abstrak Penelitian tentang profil protein sarang burung wallet masih terbatas, terutama sarang burung walet dari Indonesia. Oleh karena itu, penelitian ini bertujuan untuk menganalisa profil protein dan asam amino sarang burung walet yang berasal dari daerah Painan, Kabupaten Pesisir Selatan, Sumatera Barat. Analisis protein dilakukan dengan menggunakan SDS-PAGE, sedangkan analisis asam amino dilakukan dengan menggunakan kromatografi cair kinerja tinggi (KCKT). Analisa ekstrak air sarang burung walet dengan SDS-PAGE menunjukan bahwa sarang burung walet terdiri dari 6 protein. Keenam protein tersebut mempunyai bobot molekul masing-masing 147.2 kDa, 142.6 kDa, 133.4 kDa, 73.3 kDa, 66.2 kDa dan 37.7 kDa. Dari analisa asam amino burung walet dengan KCKT didapatkan 16 asam amino yang terkandung dalam sarang burung wallet, yang terdiri dari 7 jenis asam amino esensial yaitu Histidin (2.31%), Leusin (3.84%), Treonin (3.82%), Valin (3.93%), Metionin (0.48%), Isoleusin (1.80%), Fenilalanine (4.49%)  dan 9 asam amino non esensial yaitu Asam Serin (4.56%), Aspartat (4.48%), Arginin (3.93%), Lisin (2.34 %), Prolin (3.64%),  Asam glutamate (3.65%), Glisin (1.87%), Alanin (1.31%), Tirosin (3.92%). Serin merupakan asam amino dengan kadar tertinggi (4.56%), diikuti dengan Fenil alanine (4.49%) dan Asam aspartate (4.48%). Kandungan asam amino ini sedikit berbeda dengan kandungan asam amino sarang burung walet dari daerah dan negara lain. Kata kunci: sarang burung walet, protein, asam amino Abstract Study on protein profile of bird nest is still limited particularly protein profile of bird nest from Indonesia has not been reported. Therefore, this study was aimed to analyze protein profile and amino acid composition of bird nest from Painan, Pesisir Selatan Distric, West Sumatra. Protein analysis was performed by SDS-PAGE, and high performance liquid chromatography (HPLC) was used for amino acid analysis. SDS-PAGE analysis showed  six bands, which molecular weigh of 147.2 kDa, 142.6 kDa, 133.4 kDa, 73.3 kDa, 66.2 kDa and 37.7 kDa, respectively. On the other hand, HPLC analysis demonstrated that bird nest was composed of 16 amino acids. Seven of them were essential amino acids; histidine (2.31%), leucine (3.84%), threonine (3.82%),  valine (3.93%), methionine (0.48%), isoleucine (1.80%), phenylalanine (4.49%), and nine of them were non-essential amino acids; serine (4.56%), aspartic acid (4.48%), arginine (3.93%), lysine (2.34%), proline (3.64%), glutamic acid (3.65%), glycine (1.87%), alanine (1.31%), tyrosine (3.92%). Serine was the highest percentage of amino acid in the bird nest (4.56%), followed by phenylalanine (4.49%) and aspartic acid (4.48%). Composition of amino acid in this bird nest was slightly different with composition of amino acid in bird nest from other area. Keywords : bird nest, protein profile, amino acids

scholarly journals Tambir : Jamur Alam Papua yang Berpotensi sebagai Jamur Konsumsi

2018 ◽  
Vol 9 (1) ◽  
pp. 18-24
Author(s):  
Supeni Sufaati ◽  
Vita Purnamasari ◽  
Verena Agustini ◽  
Suharno Suharno
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
High Performance ◽  
Crude Protein ◽  
Nutrient Content ◽  
Edible Mushroom ◽  
Essential Amino Acids ◽  
Good Alternative ◽  
Local People ◽  
Alternative Protein

Fungi has several role for humankind, one of them is as food. The potency of wild edible mushrooms as a source of nutrition still need to be explored to support national food security. The aim of this study was to determine the composition of nutrient content of wild edible mushroom called Tambir that commonly be consumed as alternative food by local people in Jayapura, Papua. Samples were collected from the traditional market nearby Uncen campus in Waena, Jayapura, Papua. Micro Kjeldahl method was used to analyze the crude protein content, hexane-gravimetry for lipid, spectrophotometry for carbohydrate, and high performance liquid chromatography (HPLC) for amino acid composition. The results showed that this mushroom has 15.74 % crude protein, 2.59 % lipid, 50.17 % carbohydrate and 8 essential amino acids. The chemical value of those eight essential amino acids were more than 100 that means this mushroom has no amino acid limitation. Therefore, Tambir could be a good alternative protein source for local people. Key words: fungi, proteins, local knowledge, Tambir, Papua.

scholarly journals The Analysis of Protein Level and Amino Acid Profile in Eels (Anguilla marmorata (Q.) Gaimard and Anguilla bicolor) of Lake Poso -

2019 ◽  
Vol 9 (1) ◽  
pp. 32-37
Author(s):  
Jamaluddin ◽  
Nur Atina ◽  
Yonelian Yuyun
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Protein Content ◽  
Protein Level ◽  
High Performance ◽  
Protein Quality ◽  
Essential Amino Acids ◽  
Amino Acid Profile ◽  
Anguilla Marmorata ◽  
Two Samples

In this study used a sample of eel fish species Anguilla marmorata (Q.) Gaimard and Anguilla bicolor from Poso lake that has a high protein content and is a fish endemic to Central Sulawesi. These eels are consumed by the surrounding community of the lake, but the nutritional content remains unknown. The present research was aimed to determine the protein level and amino acid profile of Anguilla marmorata (Q.) Gaimard and Anguilla bicolor eels. The protein level testing used Kjeldahl method, and amino acid profile used High Performance Liquid Chromatography. The results demonstrate the protein level of the two samples Anguilla marmorata (Q.) Gaimard eels have a protein content of 41.84% and Anguilla bicoloreels at 33.75%. Anguilla marmorata (Q.) Gaimard and Anguilla bicolor eels contain 18 types of amino acid, comprised of 9 types of essential amino acids and 9 types of non-essential amino acids. Of the two samples of eel species Anguilla marmorata (Q.) Gaimard and Anguilla bicolor, have complete protein quality because it has all kinds of essential amino acids.

scholarly journals Turkey-Hen Amino Acid Composition of Brain and Eyes

2015 ◽  
Vol 58 (1) ◽  
pp. 30-39
Author(s):  
Emmanuel Ilesanmi Adeyeye
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Isoelectric Point ◽  
Essential Amino Acid ◽  
Meleagris Gallopavo ◽  
Dry Weight ◽  
Essential Amino Acids ◽  
Amino Acids Composition ◽  
The Brain ◽  
Total Amino Acids

The amino acids composition of the brain and eyes of the mature Turkey-hen (Meleagris gallopavo L.),  were determined on dry weight basis. Total essential amino acids ranged from 35.1-36.0 g/100 g as 49.5-49.8% of the total amino acids. The amino acid score showed that lysine ranged from 0.76-0.91 (on whole hen’s egg comparison), 0.85-1.03 (on provisional essential amino acid scoring pattern), and 0.81-0.98 (on suggested requirement of the essential amino acid of a preschool child). The predicted protein efficiency ratio was 1.94-2.41, whilst essential amino acid index range was 1.06-1.08 and the calculated isoelectric point range was 3.97-4.18. The correlation coefficient (rxy) was positively high and significant at r = 0.01 for the total amino acids, amino acid scores (on the whole hen’s egg comparisons made) and the isoelectric point. On the whole, the eyes were better in 12/18 or 66.7% parameters of the amino acids than the brain of Turkey-Hen.

scholarly journals Amino acids composition of herb of Lamiaceae family representatives

2019 ◽  
pp. 82-87
Author(s):  
M. I. Shanayda
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Amino Acid Composition ◽  
Acid Composition ◽  
Raw Materials ◽  
Essential Amino Acids ◽  
Total Amino Acid ◽  
Plant Materials ◽  
Plant Raw Materials ◽  
Amino Acids Composition

Researching of the amino acids contents in medicinal plant raw materials is an important task of pharmaceutical science and practice, because complexes of amino acids used for the corrections of hepatobiliary disorders, nervous and cardiovascular systems. The aim of this study was to conduct a qualitative and quantitative analysis of amіno acids composition of these representatives. The article presents the results of the amino acid composition investigation of the above-ground parts of 10 species belonging to Lamiaceae Juss. Family (Ocimum, Hyssopus, Dracocephalum, Lophanthus, Monarda and Satureja genera). By paper chromatography it was identified several amino acids. HPLC analyzes revealed the content and composition of more than 20 amino acids. The highest total amino acid composition was found in the herb of the genus Ocimum. It was identified 10 essential and non-essential 9 amino acids in each herb, and 3 nonproteinogenic amino acids also. It was established that in all plant materials dominate quantitatively nonessential amino acids (asparagine, proline, glutamic and aspartic acid). Among the essential amino acids arginine and threonine predominate.

scholarly journals Amino acid composition of wild yam (Dioscorea spp.)

Food Research ◽  
2019 ◽  
pp. 617-621 ◽  
Author(s):  
A. Doss ◽  
P.S. Tresina ◽  
V.R. Mohan
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Glutamic Acid ◽  
G Protein ◽  
Essential Amino Acids ◽  
Dioscorea Alata ◽  
Good Source ◽  
Amino Acids Composition ◽  
Dioscorea Species ◽  
Wild Yam

Tubers of wild yam (Dioscorea alata, D. bulbifera var. vera, D. esculenta, D. oppositifolia var. oppositifolia, D. oppositifolia var. dukhumensis, D. pentaphylla var. pentaphylla, D. spicata, D. tomentosa and D. wallichi) were analyzed for its amino acids composition. Asparatic acid and glutamic acid were found to be predominant among all the investigated Dioscorea species which ranged from 5.21 to 9.36 and 3.20 to 8.12 g/100 g protein respectively. The essential amino acids such as isoleucine, phenylalanine tyrosine, histidine, isoleucine and valine were found to be more or less higher than FAO/WHO (1991) requirement pattern. Thus, the present investigation demonstrated that the Dioscorea spp. can act as a good source of amino acid

scholarly journals Amino Acid Profiles of the Flesh of the Heterosexual Pairs of Neopetrolisthes maculatus

2017 ◽  
Vol 61 ◽  
pp. 23-35 ◽  
Author(s):  
Emmanuel Ilesanmi Adeyeye
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Essential Amino Acid ◽  
Essential Amino Acids ◽  
Efficiency Ratio ◽  
Amino Acids Composition ◽  
Amino Acid Requirements ◽  
Amino Acid Profiles ◽  
Limiting Amino Acid ◽  
Acid Index

This paper reports on amino acid profiles of the flesh of heterosexuals of porcellanids collected from the Atlantic Ocean at Orimedu beach in Ibeju-Lekki, Lagos, Nigeria. Results showed that high values of amino acids were observed in the heterosexual flesh of Neopetrolisthes maculatus (g 100g-1 protein): 17.7 – 17.8 (Glu), 9.90 – 10.0 (Asp), 8.70 – 9.07 (Arg), 7.23 – 7.94 (Leu) and 5.81 – 6.06 (Gly). Total essential amino acid values ranged from 45.2 – 46.2 g 100-1g. Predicted protein efficiency ratio was 3.82 – 4.14, the range of essential amino acid index was 86.9 – 89.9, the biological values ranged from 83.0 – 86.3. The Lys/Trp was 3.31-4.27. Serine was limiting amino acid (0.513 – 0.516) in the egg score comparison; under the essential amino acids scores, Lys (0.840) was limiting in female but Val (0.823) was limiting in the male; Lys (0.796 – 0.905) was limiting in both samples in the pre-school child amino acid requirements. It was observed that out of the twenty parameters determined, male flesh was more concentrated in 60% values than the female flesh and 40% better in female than male. Correlation coefficient result showed that significant differences existed in the amino acids composition at r = 0.01 of the N. maculatus samples.

scholarly journals Variation in protein and amino acids content among landraces of common bean (Phaseolus vulgaris L.)

2020 ◽  
pp. 750
Author(s):  
Ángel Ramón Flores-Sosa ◽  
Elia Nora Aquino-Bolaños ◽  
Anaberta Cardador-Martínez ◽  
José Luis Chávez-Servia ◽  
Araceli Minerva Vera-Guzmán ◽  
...  
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Phaseolus Vulgaris ◽  
Acid Content ◽  
High Performance ◽  
Amino Acid Content ◽  
Essential Amino Acids ◽  
Amino Acid Profile ◽  
Phaseolus Vulgaris L ◽  
Genetic Characteristics

Native bean populations (Phaseolus vulgaris L.) provide bioactive and nutrient compounds; however, their amino acid profiles are unknown. Therefore, the aim of this study is to evaluate the protein content and amino acid profile of 46 native bean populations cultivated by small farmers in Oaxaca, Mexico, and compare them with that of commercial beans. Through high-performance liquid chromatography (HPLC), 16 amino acids were identified and quantified in all samples. The region of origin influenced the concentrations of amino acids. The Santa Lucia Miahuatlan populations stood out for their high content of isoleucine, threonine, methionine, arginine, serine, alanine, tyrosine, and cysteine. Amino acid content showed high variability among the populations; accessions labeled as FSLM22, FSLM27, FSLM28, and FSLM32 were enriched in aliphatic, hydroxylated, aromatic, acidic, and basic amino acids, while the FSLM14, FSLM17, and FSLM18 populations had the highest concentrations of sulfur amino acids. The FSLM01, FSLM22, FSLM27, FSLM28, FSLM30, and FSLM32 populations frequently displayed the highest concentrations of essential amino acids. The findings show that samples of native populations are highly variable in amino acid content due to the genetic characteristics of cultivated beans, environmental and agroecological influences, and crop management by farmers. The beans populations stood out can be used for direct use or a basis for the initiation of a breeding program.

scholarly journals Amino Acids Composition of Liver, Heart and Kidneys of Thryonomys swingerianus (Temminck 1827) Compared

2020 ◽  
Vol 79 ◽  
pp. 23-39
Author(s):  
Emmanuel Ilesanmi Adeyeye ◽  
Olajide Ayodele ◽  
Joshua Iseoluwa Orege
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Essential Amino Acids ◽  
Total Amino Acid ◽  
Hen’S Egg ◽  
Amino Acids Composition ◽  
Liver And Kidney ◽  
Limiting Amino Acid ◽  
True Protein ◽  
Better Than

Amino acids composition of Thryonomysswingerianus is reported. Whereas protein values (g100g-1) were liver (74.1), kidney (91.5), heart (84.6); corresponding total amino acid values were 93.5, 83.2 and 80.6. True protein from the crude protein of the samples ran thus: liver>kidney>heart. Of the twenty parameters reported on, liver was best in 12/20 (60.0%), kidney and heart both shared the second position of 4/20(20%) each. Among the essential amino acids, leucine predominated in both liver (7.96g100g-1 protein) and kidney (8.11g100g-1 protein) but valine (6.21g100g-1 protein) predominated in the heart. The P-PER values were; P-PER1: 2.78 (liver), 2.91(kidney), 0.716 (heart) and P-PER2: 2.71 (liver), 2.90 (kidney), 0.564 (heart). However, there was a reverse between liver and kidney in the EAAI values with liver (92.0) > kidney (90.2) > heart (87.6) with corresponding BV values of 88.5 > 86.6 > 83.7. In the amino acids scoring pattern, Ser was limiting in liver (0.533) and heart (0.394) but Thr (0.490) in kidney in whole hen’s egg score comparison; in FAO/WHO scoring standards, Thr was limiting in liver (0.988) and kidney (0.625) but Leu (0.459) in heart. In pre-school requirements, liver recorded no limiting amino acid whereas Thr was limiting in kidney (0.735) and Leu was limiting in the heart (0.486). T.swingerianus red viscera was compared with the red viscera of livestock animals (cattle, sheep and pork) as well as FAO/WHO/UNU standards for total essential amino acids. Our results when compared with the livestock red viscera (without Trp) and FAO/WHO/UNU (g100g-1 protein), we have heart: grasscutter/cattle/sheep/pig:45.3 /46.0/42.7/46.6; kidney: grasscutter/cattle/sheep/pig: 47.6/43.8/42.5/46.7; liver: grasscutter/cattle/sheep/pig: 50.7/47.7/41.5/47.5 and grasscutter liver/kidney/heart/ FAO/WHO/UNU:50.7/47.6/45.3/32.8 showing that all the red viscera values in T.swingerianus were better than the essential amino acids in the FAO/WHO/UNU standards and livestock red viscera. Statistical values showed that significant differences existed among the samples at r=0.01.

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