The Delignification of Plants Residue Substrate and Accelerated Fungal Consortium Growth-Saccharification: A Practical Approach

2021 ◽  
Vol 11 (1) ◽  
pp. 63-69
Author(s):  
Ahmad Syauqi ◽  
Siti Fatimah ◽  
Durrotul Choiroh
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Growth Medium ◽  
Substrate Concentration ◽  
Raw Materials ◽  
Cell Mass ◽  
Practical Approach ◽  
Organic Substances ◽  
Fungal Consortium ◽  
Saccharification Yield ◽  
Residual Plants

The environments have created an abundance of residual plants from all life sectors, which is not optimal for bioethanol. Therefore, this research developed microbial technology that yielded sugar and fermentation testing. The research aimed to discover the delignification process and compare the consuming sugar by Saccharomyces cerevisiae between the chemical saccharification and accelerated bio-agent of fungal consortium in the engineered media. The innovation of the bioethanol process was conducted using raw materials from biomass. Based on this study, some preliminary hypotheses were made: (i) arranging fungal substrate which consists of residual sugar, molasses, and enriched residual papaya fruits could provide distinguishable growth of cell mass; (ii) the substrate concentration of 2.5% and 7.5% in the growth medium using enriched residual papaya fruits, respectively, as a medium, could be distinguished using delignification. A benchmark was used to compare the chemical and bio-agent saccharification. The consortium that grew and produced cell mass by times factor in molasses has fulfilled the element needed compared to the natural organic substances from the papaya fruit. The higher concentration of delignification material substrate yielded higher growth-saccharification and the average of 10.45 ± 0.21 % Brix was obtained by the fungal consortium in the broth medium, although the acceleration growth is insignificant. Nonetheless, Saccharomyces cerevisiae had successfully fermented saccharification yield sugar from the delignification of plants residual

scholarly journals Underproduction of the Largest Subunit of RNA Polymerase II Causes Temperature Sensitivity, Slow Growth, and Inositol Auxotrophy in Saccharomyces cerevisiae

Genetics ◽  
1996 ◽  
Vol 142 (3) ◽  
pp. 737-747 ◽  
Author(s):  
Jacques Archambault ◽  
David B Jansma ◽  
James D Friesen
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Steady State ◽  
Rna Polymerase ◽  
Rna Polymerase Ii ◽  
Reporter Gene ◽  
Growth Medium ◽  
Temperature Sensitivity ◽  
Slow Growth ◽  
Yeast Saccharomyces Cerevisiae ◽  
Genes Encoding

Abstract In the yeast Saccharomyces cerevisiae, mutations in genes encoding subunits of RNA polymerase II (RNAPII) often give rise to a set of pleiotropic phenotypes that includes temperature sensitivity, slow growth and inositol auxotrophy. In this study, we show that these phenotypes can be brought about by a reduction in the intracellular concentration of RNAPII. Underproduction of RNAPII was achieved by expressing the gene (RPO21), encoding the largest subunit of the enzyme, from the LEU2 promoter or a weaker derivative of it, two promoters that can be repressed by the addition of leucine to the growth medium. We found that cells that underproduced RPO21 were unable to derepress fully the expression of a reporter gene under the control of the INO1 UAS. Our results indicate that temperature sensitivity, slow growth and inositol auxotrophy is a set of phenotypes that can be caused by lowering the steady-state amount of RNAPII; these results also lead to the prediction that some of the previously identified RNAPII mutations that confer this same set of phenotypes affect the assembly/stability of the enzyme. We propose a model to explain the hypersensitivity of INO1 transcription to mutations that affect components of the RNAPII transcriptional machinery.

scholarly journals Influence of organic acids and organochlorinated insecticides on metabolism of Saccharomyces cerevisiae

2005 ◽  
pp. 207-215 ◽  
Author(s):  
Dusanka Pejin ◽  
Vesna Vasic
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Growth Rate ◽  
Sugar Beet ◽  
Specific Growth Rate ◽  
Glycogen Content ◽  
Specific Growth ◽  
Raw Materials ◽  
Stress Factors ◽  
Adaptive Mechanism ◽  
Ribonucleic Acids

Saccharomyces cerevisiae is exposed to different stress factors during the production: osmotic, temperature, oxidative. The response to these stresses is the adaptive mechanism of cells. The raw materials Saccharomyces cerevisiae is produced from, contain metabolism products of present microorganisms and protective agents used during the growth of sugar beet for example the influence of acetic and butyric acid and organochlorinated insecticides, lindan and heptachlor, on the metabolism of Saccharomyces cerevisiae was investigated and presented in this work. The mentioned compounds affect negatively the specific growth rate, yield, content of proteins, phosphorus, total ribonucleic acids. These compounds influence the increase of trechalose and glycogen content in the Saccharomyces cerevisiae cells.

Effect of Different Ending Fermentation Technologies on Microbial-Stability of Italian Riesling Low Alcohol Sweet White Wine

2011 ◽  
Vol 393-395 ◽  
pp. 1165-1168 ◽  
Author(s):  
Yan Cui ◽  
Wen Lv ◽  
Jin Fu Liu ◽  
Bu Jiang Wang
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Electrostatic Field ◽  
Raw Materials ◽  
White Wine ◽  
Grape Variety ◽  
Lethal Rate ◽  
Microbial Stability ◽  
Fermentation Technology ◽  
Low Alcohol

In order to investigate effects of different ending fermentation technologies on microbial-stability and quality of low alcohol sweet white wine, with Italy Reasling grapes as raw materials, four ending fermentation technologies: single SO2 treatment, combined high voltage electrostatic field (HVEF)/SO2 treatment, combined ultrasound/SO2 treatment, and combined pasteurization/SO2 treatment were compared in yeasts lethality, enological parameters and sensory evaluation of the low-alcohol sweet white wines.The results showed that: the best ending fermentation technology was combined ultrasound (40Hz/20min)/SO2(40mg/L) treatment, which had higher total lethal rate of Saccharomyces cerevisiae, and the wines treated in this way were rich in typical Italy Reasling grape variety flavor and attractive aromas, with a pleasant fruity taste and microbial stability.

scholarly journals Improved Phenolic Compositions and Sensory Attributes of Red Wines by Saccharomyces cerevisiae Mutant CM8 Overproducing Cell-Wall Mannoproteins

Processes ◽  
2020 ◽  
Vol 8 (11) ◽  
pp. 1483
Author(s):  
Phoency F.-H. Lai ◽  
Po-Chun Hsu ◽  
Bo-Kang Liou ◽  
Rupesh D. Divate ◽  
Pei-Ming Wang ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Cell Wall ◽  
Red Wine ◽  
Raw Materials ◽  
Principal Component ◽  
Consumer Preference ◽  
Quality Attributes ◽  
Sensory Attributes ◽  
Red Wines ◽  
High Mannose

The objective of this study was to improve the quality attributes of red wines by Saccharomyces cerevisiae (BCRC 21685) mutant CM8 with overexpression of high-mannose mannoproteins, with respective to phenolic compositions, colorimetric parameters, and consumer sensory attributes. The CM8 was mutated by ethyl methane sulfonate and showed the ability of overproducing cell wall mannoproteins selected by killer-9 toxin-containing YPD plates. Kyoho grapes were used as raw materials. It is interesting to find that the cell wall mannoproteins isolated from CM8 mutant possessed a significantly higher mannose content in the polysaccharide fraction (81% w/w) than that did from parent strain (66% w/w). The red wines made of winter grapes and CM8 (CM8-WIN) showed significantly greater total tannins, flavonols, and anthocyanins levels, as well as higher color, higher flavor, and higher consumer preference than those by its SC counterpart (SC-WIN). The characteristics of the red wines studied were further elucidated by principal component analysis. Conclusively, using CM8 starter could effectively endow the red wine with high-quality attributes via the interactions of high-mannose mannoproteins with wine compounds.

scholarly journals Metabolic Engineering of Saccharomyces cerevisiae for Conversion of d-Glucose to Xylitol and Other Five-Carbon Sugars and Sugar Alcohols

2007 ◽  
Vol 73 (17) ◽  
pp. 5471-5476 ◽  
Author(s):  
Mervi H. Toivari ◽  
Laura Ruohonen ◽  
Andrei N. Miasnikov ◽  
Peter Richard ◽  
Merja Penttilä
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Metabolic Engineering ◽  
Growth Medium ◽  
Fold Increase ◽  
Pichia Stipitis ◽  
Xylitol Dehydrogenase ◽  
Sugar Alcohols ◽  
Pentose Sugar ◽  
Encoding Gene ◽  
Pentose Sugars

ABSTRACT Recombinant Saccharomyces cerevisiae strains that produce the sugar alcohols xylitol and ribitol and the pentose sugar d-ribose from d-glucose in a single fermentation step are described. A transketolase-deficient S. cerevisiae strain accumulated d-xylulose 5-phosphate intracellularly and released ribitol and pentose sugars (d-ribose, d-ribulose, and d-xylulose) into the growth medium. Expression of the xylitol dehydrogenase-encoding gene XYL2 of Pichia stipitis in the transketolase-deficient strain resulted in an 8.5-fold enhancement of the total amount of the excreted sugar alcohols ribitol and xylitol. The additional introduction of the 2-deoxy-glucose 6-phosphate phosphatase-encoding gene DOG1 into the transketolase-deficient strain expressing the XYL2 gene resulted in a further 1.6-fold increase in ribitol production. Finally, deletion of the endogenous xylulokinase-encoding gene XKS1 was necessary to increase the amount of xylitol to 50% of the 5-carbon sugar alcohols excreted.

Studies in intermicrobial symbiosis. Saccharomyces cerevisiae and Lactobacillus casei

1972 ◽  
Vol 18 (11) ◽  
pp. 1733-1742 ◽  
Author(s):  
R. D. Megee III ◽  
J. F. Drake ◽  
A. G. Fredrickson ◽  
H. M. Tsuchiya
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Continuous Culture ◽  
Mixed Culture ◽  
Growth Medium ◽  
Lactobacillus Casei ◽  
Mixed Cultures ◽  
Genetic Characteristics ◽  
Culture Techniques ◽  
Pure Cultures

Saccharomyces cerevisiae and a riboflavin assay strain of Lactobacillus casei have been propagated anaerobically in mixed culture. Both batch and continuous culture techniques were used. By varying the concentrations of glucose and riboflavin in the growth medium, it was possible to produce symbioses of commensalism + competition, competition, and mutualism + competition. In short, the interaction prevailing is determined by the medium as well as by the genetic characteristics of the organisms. The behavior of the mixed cultures in these situations was predicted from data taken on pure cultures of the organisms.

Regulation of phosphoenolpyruvate carboxykinase and pyruvate kinase in Saccharomyces cerevisiae grown in the presence of glycolytic and gluconeogenic carbon sources and the role of mitochondrial function on gluconeogenesis

1986 ◽  
Vol 32 (12) ◽  
pp. 969-972 ◽  
Author(s):  
Albert J. Wilson ◽  
J. K. Bhattacharjee
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Pyruvate Kinase ◽  
Growth Medium ◽  
Phosphoenolpyruvate Carboxykinase ◽  
Carbon Sources ◽  
Absolute Requirement ◽  
Fructose 1,6 Bisphosphatase ◽  
Futile Cycling ◽  
Respiratory Deficient

Phosphoenolpyruvate carboxykinase (PEPCKase) and pyruvate kinase (PKase) were measured in Saccharomyces cerevisiae grown in the presence of glycolytic and gluconeogenic carbon sources. The PEPCKase activity was highest in ethanol-grown cells. However, high PEPCKase activity was also observed in cells grown in 1% glucose, especially as compared with the activity of sucrose-, maltose-, or galactose-grown cells. Activity was first detected after 12 h when glucose was exhausted from the growth medium. The PKase activity was very high in glucose-grown cells; considerable activity was also present in ethanol- and pyruvate-grown cells. The absolute requirement of respiration for gluconeogenesis was demonstrated by the absence or significantly low levels of PEPCKase and fructose-1,6-bisphosphatase activities observed in respiratory deficient mutants, as well as in wild-type S. cerevisiae cells grown in the presence of glucose and antimycin A or chloramphenicol. Obligate glycolytic and gluconeogenic enzymes were present sumultaneously only in stationary phase cells, but not in exponential phase cells; hence futile cycling could not occur in log phase cells regardless of the presence of carbon source in the growth medium.

scholarly journals Improved Anaerobic Use of Arginine by Saccharomyces cerevisiae

2003 ◽  
Vol 69 (3) ◽  
pp. 1623-1628 ◽  
Author(s):  
Olga Martin ◽  
Marjorie C. Brandriss ◽  
Gisbert Schneider ◽  
Alan T. Bakalinsky
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Nitrogen Source ◽  
Genetically Modified ◽  
Cell Mass ◽  
Sole Nitrogen Source ◽  
Mitochondrial Targeting ◽  
Arginine Catabolism

ABSTRACT Anaerobic arginine catabolism in Saccharomyces cerevisiae was genetically modified to allow assimilation of all four rather than just three of the nitrogen atoms in arginine. This was accomplished by bypassing normal formation of proline, an unusable nitrogen source in the absence of oxygen, and causing formation of glutamate instead. A pro3 ure2 strain expressing a PGK1 promoter-driven PUT2 allele encoding Δ1-pyrroline-5-carboxylate dehydrogenase lacking a mitochondrial targeting sequence produced significant cytoplasmic activity, accumulated twice as much intracellular glutamate, and produced twice as much cell mass as the parent when grown anaerobically on limiting arginine as sole nitrogen source.

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