scholarly journals Microbial mediation of textures and minerals – terrestrial or parent body processes?

2019 ◽  
Vol 28 (1) ◽  
pp. 40-60
Author(s):  
Márta Polgári ◽  
Ildikó Gyollai ◽  
Szaniszló Bérczi ◽  
Miklós Veres ◽  
Arnold Gucsik ◽  
...  

Abstract Evolution of chondritic parent body is influenced by thermal, impact metamorphism and aqueous alteration, studied in Mező-Madaras, Knyahinya, Mócs and Nyírábrány in aspect of high resolution in situ textural, mineralogical and organic geochemical characteristics, using optical microscopy, FTIR-ATR and Raman spectroscopy. Our observations focused on Fe-containing opaque grains, glass, olivines and pyroxenes, which were well populated by micrometer-sized microbial filamentous elements in their boundary region within matrix and inside the minerals resembling mineralized microbially produced textures (MMPT), affecting 70-80 vol% of samples. In MMPT iron oxides (ferrihydrite, goethite), olivine, montmorillonite, kandite minerals and various hydrocarbon compounds were identified. (1) Data confirmed dense and invasive terrestrial microbially mediated contamination in the chondrites, supported by microtexture, micromineralogy and embedded organic compounds. As the classical transformation processes are supposed nowadays to have been happened on the parent bodies, a contradiction arose: how could it be that these classical products are manifested in microbially mediated texture? (2) Based on terrestrial analogies, microbial mediation is a sudden process comparing to geological times, very ancient, widespread and occur in various environments under determined conditions. It can consume previous and also produce new minerals. After formation, MMPT can survive billions of years proposing occurrence on parent bodies.

2014 ◽  
Vol 131 ◽  
pp. 368-392 ◽  
Author(s):  
Corentin Le Guillou ◽  
Sylvain Bernard ◽  
Adrian J. Brearley ◽  
Laurent Remusat

2018 ◽  
Vol 222 ◽  
pp. 230-252 ◽  
Author(s):  
Christine E. Jilly-Rehak ◽  
Gary R. Huss ◽  
Kazu Nagashima ◽  
Devin L. Schrader

Author(s):  
J. A. Pollock ◽  
M. Martone ◽  
T. Deerinck ◽  
M. H. Ellisman

Localization of specific proteins in cells by both light and electron microscopy has been facilitate by the availability of antibodies that recognize unique features of these proteins. High resolution localization studies conducted over the last 25 years have allowed biologists to study the synthesis, translocation and ultimate functional sites for many important classes of proteins. Recently, recombinant DNA techniques in molecular biology have allowed the production of specific probes for localization of nucleic acids by “in situ” hybridization. The availability of these probes potentially opens a new set of questions to experimental investigation regarding the subcellular distribution of specific DNA's and RNA's. Nucleic acids have a much lower “copy number” per cell than a typical protein, ranging from one copy to perhaps several thousand. Therefore, sensitive, high resolution techniques are required. There are several reasons why Intermediate Voltage Electron Microscopy (IVEM) and High Voltage Electron Microscopy (HVEM) are most useful for localization of nucleic acids in situ.


Author(s):  
Gary Bassell ◽  
Robert H. Singer

We have been investigating the spatial distribution of nucleic acids intracellularly using in situ hybridization. The use of non-isotopic nucleotide analogs incorporated into the DNA probe allows the detection of the probe at its site of hybridization within the cell. This approach therefore is compatible with the high resolution available by electron microscopy. Biotinated or digoxigenated probe can be detected by antibodies conjugated to colloidal gold. Because mRNA serves as a template for the probe fragments, the colloidal gold particles are detected as arrays which allow it to be unequivocally distinguished from background.


1987 ◽  
Vol 48 (C7) ◽  
pp. C7-761-C7-762
Author(s):  
B. LAVOREL ◽  
G. MILLOT ◽  
R. SAINT-LOUP ◽  
M. L. GONZE ◽  
J. SANTOS ◽  
...  

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