scholarly journals The In Situ Human Dental Implantome: A First Appraisal

2021 ◽  
Vol 7 (2) ◽  
pp. 827-830
Author(s):  
Andrea Sowislok ◽  
Thomas Weischer ◽  
Herbert P. Jennissen
Keyword(s):  
Serum Albumin ◽  
Protein Adsorption ◽  
Dental Implant ◽  
Human Body ◽  
Total Abundance ◽  
Fundamental Interaction ◽  
Free Hemoglobin ◽  
Selective Enrichment ◽  
Foreign Surface

Abstract Protein adsorption is the first fundamental interaction between the human body and a foreign surface. The sum of all proteins in this adherent proteinaceous layer comprises the implant proteome. The in situdental implant proteome (implantome) was eluted from four implants of two wettabilities after a 2-min dipping in the humor operationis of maxillar tooth sockets. A mean number of 2056 different polypeptides per implant were identified according to the Xcorr method (Xcorr score ≥ 1.5, n ≥ 2 peptides). In the top 12 proteins comprising ca. 47% of the total abundance, cell-free hemoglobin (26.7%) was the most abundant, followed by fibrinogen (6.4%) and serum albumin (1.8%) with additional 1,800 lower abundance polypeptides, which contained ca. 34 salivary and a similar number of autoimmunogenic polypeptides. Selective enrichment of cell-free hemoglobin on the implant vs. albumin was estimated to 270 fold

scholarly journals Development of In Situ Gelling Meloxicam-Human Serum Albumin Nanoparticle Formulation for Nose-to-Brain Application

Pharmaceutics ◽  
2021 ◽  
Vol 13 (5) ◽  
pp. 646
Author(s):  
Gábor Katona ◽  
Bence Sipos ◽  
Mária Budai-Szűcs ◽  
György Tibor Balogh ◽  
Szilvia Veszelka ◽  
...  
Keyword(s):  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Nasal Mucosa ◽  
Drug Absorption ◽  
Cell Damage ◽  
Lipid Fraction ◽  
Poloxamer 407 ◽  
Nasal Drug Delivery

The aim of this study was to develop an intranasal in situ thermo-gelling meloxicam-human serum albumin (MEL-HSA) nanoparticulate formulation applying poloxamer 407 (P407), which can be administered in liquid state into the nostril, and to increase the resistance of the formulation against mucociliary clearance by sol-gel transition on the nasal mucosa, as well as to improve drug absorption. Nanoparticle characterization showed that formulations containing 12–15% w/w P407 met the requirements of intranasal administration. The Z-average (in the range of 180–304 nm), the narrow polydispersity index (PdI, from 0.193 to 0.328), the zeta potential (between −9.4 and −7.0 mV) and the hypotonic osmolality (200–278 mOsmol/L) of MEL-HSA nanoparticles predict enhanced drug absorption through the nasal mucosa. Based on the rheological, muco-adhesion, drug release and permeability studies, the 14% w/w P407 containing formulation (MEL-HSA-P14%) was considered as the optimized formulation, which allows enhanced permeability of MEL through blood–brain barrier-specific lipid fraction. Cell line studies showed no cell damage after 1-h treatment with MEL-HSA-P14% on RPMI 2650 human endothelial cells’ moreover, enhanced permeation (four-fold) of MEL from MEL-HSA-P14% was observed in comparison to pure MEL. Overall, MEL-HSA-P14% can be promising for overcoming the challenges of nasal drug delivery.

In situ fabrication of PHEMA–BSA core–corona biohybrid particles

2016 ◽  
Vol 4 (25) ◽  
pp. 4430-4438 ◽  
Author(s):  
Jin-Tao Wang ◽  
Yanhang Hong ◽  
Xiaotian Ji ◽  
Mingming Zhang ◽  
Li Liu ◽  
...  
Keyword(s):  
Electron Transfer ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Atom Transfer ◽  
Hydroxyethyl Methacrylate ◽  
In Situ Fabrication ◽  
Radical Polymerizations

Poly(2-hydroxyethyl methacrylate)–bovine serum albumin core–corona particles were prepared using in situ activators generated by electron transfer for atom transfer radical polymerizations of HEMA initiated by a BSA macroinitiator.

scholarly journals Plasmon-enhanced two-channel in situ Kretschmann ellipsometry of protein adsorption, cellular adhesion and polyelectrolyte deposition on titania nanostructures

2016 ◽  
Vol 24 (5) ◽  
pp. 4812 ◽  
Author(s):  
Judit Nador ◽  
Benjamin Kalas ◽  
Andras Saftics ◽  
Emil Agocs ◽  
Peter Kozma ◽  
...  
Keyword(s):  
Protein Adsorption ◽  
Cellular Adhesion ◽  
Titania Nanostructures

An in situ study of protein adsorption on combinatorial Cu–Al films using spectroscopic ellipsometry

2010 ◽  
Vol 81 (1) ◽  
pp. 58-66 ◽  
Author(s):  
M.A. McArthur ◽  
T.M. Byrne ◽  
R.J. Sanderson ◽  
G.P. Rockwell ◽  
L.B. Lohstreter ◽  
...  
Keyword(s):  
Protein Adsorption ◽  
Spectroscopic Ellipsometry ◽  
In Situ Study

scholarly journals Unique dynamic mode between Artepillin C and human serum albumin implies the characteristics of Brazilian green propolis representative bioactive component

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Fan Wu ◽  
Xin-Mi Song ◽  
Yi-Lei Qiu ◽  
Huo-Qing Zheng ◽  
Fu-Liang Hu ◽  
...  
Keyword(s):  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Human Body ◽  
Mode Interaction ◽  
Dynamic Mode ◽  
Bioactive Component ◽  
Artepillin C ◽  
Functional Components ◽  
Brazilian Green Propolis

Abstract As a representative bioactive component in Brazil green propolis, Artepillin C (ArtC; 3, 5-diprenyl-4-hydroxycinnamic acid) has been reported a wide variety of physiological activities including anti-tumor, anti-inflammatory, and antimicrobial activity etc. However, it seems incompatible that ArtC in vivo was characterized as low absorption efficiency and low bioavailability. In order to obtain the elucidation, we further investigated the physicochemical basis of ArtC interacting with human serum albumin (HSA) in vitro. We found a unique dynamic mode interaction between ArtC and HSA, which is completely different from other reported propolis bioactive components. Thermodynamic analysis showed that hydrophobic interactions and electrostatic forces are the main driving force. The competitive assay indicates that the binding site of ArtC with HSA is close to the Sudlow’s site I. The findings of this study reveal the unique physicochemical transport mechanism of ArtC in the human body, which helps to further understand the uniqueness of the representative functional components of Brazilian green propolis in the human body.

Influence of Human Serum Albumin Glycation on the Binding Affinities for Natural Flavonoids

2019 ◽  
Vol 17 (1) ◽  
pp. 806-812
Author(s):  
Liangliang Liu ◽  
Yi Liu ◽  
Aiping Xiao ◽  
Shiyong Mei ◽  
Yixi Xie
Keyword(s):  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Small Molecules ◽  
Human Body ◽  
Binding Sites ◽  
Plasma Proteins ◽  
Fluorescence Spectra ◽  
Binding Affinities ◽  
Dietary Flavonoids

AbstractIncreasing the degree of glycation in diabetes could affect the ability of plasma proteins in binding to small molecules and active compounds. In this study, the influence of glycation of Human serum albumin (HSA) on the binding affinities for six dietary flavonoids was investigated by fluorescence spectra. Glycated HSA was prepared through incubation with glucose and characterized by several methods to confirm the glycation. It was found that the level of glycation increased with the increasing incubation time. The glycation of HSA increased the binding affinities for flavonoids by 1.40 to 48.42 times, which indicates that modifications caused by the glycation may have different influences on the interactions of flavonoids with HSA at separate binding sites on this protein. These results are valuable for understanding the influence of diabetes on the metabolism of flavonoids and other bioactive small molecules in human body.

scholarly journals Fabrication of a Novel Antifouling Polysulfone Membrane with in Situ Embedment of Mxene Nanosheets

2019 ◽  
Vol 16 (23) ◽  
pp. 4659 ◽  
Author(s):  
Zhen Shen ◽  
Wei Chen ◽  
Hang Xu ◽  
Wen Yang ◽  
Qing Kong ◽  
...  
Keyword(s):  
Contact Angle ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Water Contact Angle ◽  
Bovine Serum ◽  
Composite Membranes ◽  
Recovery Ratio ◽  
Water Contact ◽  
Polysulfone Membrane

Membrane fouling is still a critical issue for the application of ultrafiltration, which has been widely used in water treatment due to its efficiency and simplicity. In order to improve the antifouling property, a new 2D material MXene was used to fabricate composite ultrafiltration membrane with the approach of in situ embedment during the phase inversion process in this study. Scanning electron microscopy (SEM), atomic force microscopy (AFM), thermogravimetric analysis (TGA), energy dispersive spectroscopy (EDS), Fourier transform infrared spectroscopy (FTIR), water contact angle, bovine serum albumin rejection and porosity measurements were utilized to characterize the prepared membranes. Due to the hydrophilicity of the MXene, the composite membranes obtained higher hydrophilicity, confirmed by the decreased water contact angle. All the modified membranes had a high bovine serum albumin rejection above 90% while that of the pristine polysulfone membrane was 77.48%. The flux recovery ratio and the reversible fouling ratio of the membranes were also improved along with the increasing content of the MXene. Furthermore, the highest flux recovery ratio could also reach 76.1%. These indicated the good antifouling properties of MXene composite membranes. The enhanced water permeability and protein rejection and excellent antifouling properties make MXene a promising material for antifouling membrane modification.

Emerging investigator series: protein adsorption and transformation on catalytic and food-grade TiO2 nanoparticles in the presence of dissolved organic carbon

2019 ◽  
Vol 6 (6) ◽  
pp. 1688-1703 ◽  
Author(s):  
Junyeol Kim ◽  
Kyle Doudrick
Keyword(s):  
Titanium Dioxide ◽  
Bovine Serum Albumin ◽  
Organic Carbon ◽  
Serum Albumin ◽  
Surface Chemistry ◽  
Protein Adsorption ◽  
Bovine Serum ◽  
Titanium Dioxide Nanoparticles ◽  
Food Grade ◽  
Exposure History

The adsorption and unfolding behavior of bovine serum albumin onto catalytic- and food-grade titanium dioxide nanoparticles is dependent on the surface chemistry of the nanoparticles and their environmental exposure history.

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