scholarly journals Characterization of a new toxin from the entomopathogenic fungus Metarhizium anisopliae: the ribotoxin anisoplin

2017 ◽  
Vol 398 (1) ◽  
pp. 135-142 ◽  
Author(s):  
Miriam Olombrada ◽  
Pilar Medina ◽  
Flor Budia ◽  
José G. Gavilanes ◽  
Álvaro Martínez-del-Pozo ◽  
...  
Keyword(s):  
Recombinant Protein ◽  
Virulence Factors ◽  
Malaria Vector ◽  
Metarhizium Anisopliae ◽  
Entomopathogenic Fungus ◽  
Insect Cells ◽  
Malaria Vector Control ◽  
Ribonucleolytic Activity ◽  
Hirsutella Thompsonii

Abstract Metarhizium anisopliae is an entomopathogenic fungus relevant in biotechnology with applications like malaria vector control. Studies of its virulence factors are therefore of great interest. Fungal ribotoxins are toxic ribonucleases with extraordinary efficiency against ribosomes and suggested as potential insecticides. Here we describe this ribotoxin characteristic activity in M. anisopliae cultures. Anisoplin has been obtained as a recombinant protein and further characterized. It is structurally similar to hirsutellin A, the ribotoxin from the entomopathogen Hirsutella thompsonii. Moreover, anisoplin shows the ribonucleolytic activity typical of ribotoxins and cytotoxicity against insect cells. How Metarhizium uses this toxin and possible applications are of interest.

Expression and characterization of the 42 kDa chitinase of the biocontrol fungusMetarhizium anisopliaeinEscherichia coli

2003 ◽  
Vol 49 (11) ◽  
pp. 723-726 ◽  
Author(s):  
César Milton Baratto ◽  
Marcia Vanusa da Silva ◽  
Lucélia Santi ◽  
Luciane Passaglia ◽  
Irene Silveira Schrank ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Recombinant Protein ◽  
Metarhizium Anisopliae ◽  
Entomopathogenic Fungus ◽  
Expression Vector ◽  
Hydrolytic Enzymes ◽  
Active Protein ◽  
Chitinase Genes

Albeit Metarhizium anisopliae is the best-characterized entomopathogenic fungus, the role of some hydrolytic enzymes during host cuticle penetration has not yet been established. Three chitinase genes (chit1, chi2, chi3) from Metarhizium have already been isolated. To characterize the chitinase coded by the chit1 gene, we expressed the active protein (CHIT42) in Escherichia coli using a T7-based promoter expression vector. The recombinant protein, CHIT42, is active against glycol chitin and synthetic N-acetylglucosamine (GlcNAc) dimer and tetramer substrates. These activities suggest that the recombinant CHIT42 acts as an endochitinase.Key words: Metarhizium anisopliae, chitinases, chit genes, recombinant protein, enthomopathogenic fungi.

Characterization of isolates of the entomopathogenic fungus Metarhizium anisopliae by RAPD-PCR

1994 ◽  
Vol 98 (9) ◽  
pp. 1077-1081 ◽  
Author(s):  
S.C.M. Leal ◽  
D.J. Bertioli ◽  
T.M. Butt ◽  
J.F. Peberdy
Keyword(s):  
Metarhizium Anisopliae ◽  
Entomopathogenic Fungus ◽  
Rapd Pcr
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