Antioxidant Metabolites from Limonium brasiliense (Boiss.) Kuntze

2004 ◽  
Vol 59 (7-8) ◽  
pp. 477-480 ◽  
Author(s):  
Ana P. Murray ◽  
Silvana Rodriguez ◽  
María A. Frontera ◽  
María A. Tomas ◽  
María C. Mulet
Keyword(s):  
Chemical Constituents ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Spectroscopic Methods ◽  
H Nmr ◽  
Antioxidant Metabolites ◽  
First Time ◽  
C Nmr

Abstract A free radical scavenging activity guided fractionation of the polar extract from roots of Limonium brasiliense (Plumbaginaceae) led to the isolation of five active compounds including: myricetin 3-O-α-rhamnopyranoside (1), (-)-epigallocatechin 3-O-gallate (2), (-)-epigallocatechin (3), (+)-gallocatechin (4) and gallic acid (5). These and other chemical constituents are reported for the first time for this species. The characterization of these compounds was achieved by spectroscopic methods (1H NMR, 13C NMR and UV).

scholarly journals Characterization of Szovitsia callicarpa Volatile Constituents Obtained by Micro- and Hydrodistillation

2010 ◽  
Vol 5 (2) ◽  
pp. 1934578X1000500 ◽  
Author(s):  
Betül Demirci ◽  
Nurgün Küçükboyacı ◽  
Nezaket Adıgüzel ◽  
K. Hüsnü Can Başer ◽  
Fatih Demirci
Keyword(s):  
Essential Oil ◽  
Plant Material ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Major Constituent ◽  
Spectroscopic Techniques ◽  
Volatile Constituents ◽  
C Nmr

The volatile constituents of Szovitsia callicarpa Fisch. & C. A. Mey. were obtained from the fruits by microdistillation, due to the limited plant material availability. Initial GC-MS analyses of the obtained material showed an unidentified major constituent with a molecular ion peak at M+ 280. After hydrodistillation of the plant material, the resulting essential oil was also analyzed by GC and GC-MS, simultaneously. In total, twenty-two compounds, representing 98.6% of the microdistilled sample, were identified, whereas seventeen components were detected in the hydrodistilled oil, representing 98.5% of the total. The GC-MS analyses showed that the samples contained an unidentified major constituent, which was further purified from the hydrodistilled essential oil by micro-column chromatography. The structure was elucidated as α-kessyl acetate using 1H- and 13C-NMR spectroscopic and mass spectroscopic techniques. Other major constituents in the analytes were determined as longipinene, longicyclene and kessane. In addition, both samples, as well as the major compound α-kessyl acetate, were tested at 1 mg/mL concentration against the pathogen Candida tropicalis and for its free radical scavenging activity against 2,2-diphenyl-1-picrylhydrazyl (DPPH•) by TLC-bioauthographic techniques. Both preliminary assays at the tested concentration showed no activity.

A New Hepatoprotective Flavone Glycoside from the Flowers of Onopordum alexandrinum Growing in Egypt

2011 ◽  
Vol 66 (5-6) ◽  
pp. 251-259 ◽  
Author(s):  
Maha M. Salama ◽  
Shahira M. Ezzat ◽  
Amany A. Sleem
Keyword(s):  
Free Radical ◽  
Cell Damage ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Ethyl Acetate Fraction ◽  
Hepatoprotective Activity ◽  
Free Radical Scavenging ◽  
Flavone Glycoside ◽  
H Nmr ◽  
C Nmr

A bioactivity-guided fractionation of the ethyl acetate fraction of the flowers of Onopordum alexandrinum L. (Asteraceae) yielded a new flavonoidal glycoside designated as acacetin-7-O-galacturonide (9), alongside with nine known flavonoids; 6-methoxy-apigenin (hispidulin) (1), acacetin (2), apigenin (3), luteolin (4), kaempferol (5), eriodictyol (6), apigenin- 7-O-glucoside (7), luteolin-7-O-glucoside (8), and kaempferol-3-O-rutinoside (10). The compounds were assayed for their hepatoprotective activity against CCl4-induced hepatic cell damage in rats and free radical scavenging activity using 2,2-diphenyl-1-picrylhydrazyl (DPPH). Compounds 4, 6, 9, and 10 have not been previously reported from flowers of O. alexandrinum L., and this is the first report of acacetin-7-O-galacturonide (9) in nature which has also shown significant hepatoprotective and free radical scavenging effects. The isolated compounds were identified using different spectroscopic methods (UV, 1H NMR, 13C NMR, HMQC, HMBC, and COSY).

scholarly journals A Natural Triglyceride from the Methanol Root Extract of Cyphostemma Adenocaule (Steud. Ex A. Rich.) Wild & R.B.Drumm

2020 ◽  
Author(s):  
Abdulbasit Haliru Yakubu ◽  
Iliya Ibrahim ◽  
Abdulqadir bukar bababe ◽  
Hassan Yesufu ◽  
mohammed Garba Tom
Keyword(s):  
Mobile Phase ◽  
Nmr Spectra ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Solvent System ◽  
Root Extract ◽  
H Nmr ◽  
Signal Peak ◽  
Methylene Group ◽  
C Nmr

<p><i>Cyphostemma adenocaule </i>(Steud. ex A. Rich.) is one of the specie plant that belongs to the family vitacea. In this study, Trilinolein was isolated and characterized from the methanol root extract of the plant. Column chromatography over silica gel granules as the stationary phase and eluted with a mobile phase mixture of n-Hex-EtA; EtA-CHCL3 and CHCL<sub>3</sub>-MeOH with gradient increasing polarity, followed by a second column using saphadex-LH20 and 100% MeOH as stationary and mobile phase vehicle respectively. TLC was developed with EtA 15: CHCL3<sub> </sub>8: MeOH 4: H<sub>2</sub>O 1 as solvent system; sprayed with 10% H<sub>2</sub>SO<sub>4 </sub>,Vanillin-sulphuric acid, and/ or Polyethylene glycol PEG and heat for spot detection and confirmation of bioactive principles. Compound CA1 was obtained and purified with CHCL3 to give a yellow semi-solid compound (0.23g). The <sup>1</sup>H-NMR spectra showed 9 different signals; a signal peak of a glycerol (-C<b>H<sub>2</sub></b>OCOR-) moiety on the first α-C chain and on the third αʹ-C at 4.143-4.187ppm and 4.296-4.325ppm respectively, while that of a β glycerol (-C<b>H</b>COR-) at 5.286ppm. Signals of an allylic methylene group at 2.023-2.035ppm, Olefenic hydrogen group at signal peak of 5.362ppm and a diallylic methylene group at signal 2.790ppm were also observed. In the <sup>13</sup>C NMR spectra of compound CA1, 57 carbon atoms where observed, multiple signals overlapping at a range of 14.13-34.21ppm corresponding to the aliphatic CH3 (<b>C18</b>), CH2 (<b>C2, C3, C4, C5, C6, C7, C15, C16, and C17</b>) and allylic (<b>C8, C14</b>) carbon atoms. Signals at 127.90-130.24ppm were assigned to the olefienic C atoms (<b>C9, C10, C12</b>, and <b>C13</b>) while signal of 172.87ppm and 173.32ppm were assigned to the carbonyl (<b>C</b>=O) carbon atoms (<b>C1 </b>and<b> C2</b>) respectively (Table 2). </p> <p>Analysis with DEPT-135, H-H COSY, HMBC and HSQC assignments of CA1 augments assignment of signals made for CA1 from <sup>1</sup>H-NMR and <sup>13</sup>C-NMR and corresponded to that of Trilinolein <u>(<a href="https://pubchem.ncbi.nlm.nih.gov/#query=C57H98O6">C<sub>57</sub>H<sub>98</sub>O<sub>6</sub></a>, </u>MW 879.4 g/mol). The isolated compound was positive for the acrolein test for triglycerides; fat & oil and had an IC<sub>50</sub> of 46.08µg/ml radical scavenging activity.</p>

scholarly journals Isolation and Characterization of Antimicrobial Compound from Stem Bark of Traditional Medicinal Plant Sonneratia apetala: Evaluation of their antimicrobial, Cytotoxic and Antioxidant Properties

2021 ◽  
Vol 38 (1) ◽  
pp. 1-5
Author(s):  
Md Arifur Rahman ◽  
Ahmed Abu Rus’d ◽  
Md Enamul Haque
Keyword(s):  
Brine Shrimp ◽  
Soluble Fraction ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Stem Bark ◽  
Chloroform Extract ◽  
Free Radical Scavenging ◽  
Gram Negative Bacteria ◽  
Isolation And Characterization

Sonneratia apetala (S. apetala) (Lythraceae) has been investigated for the isolation and characterization of antimicrobial compounds and evaluation of their biological activities. The chloroform extract of the stem bark and different partitionate of the chloroform extracts i.e. Petroleum ether soluble fraction (PESF), Ethyl acetate soluble fraction(EASF), Methanol soluble fraction(MSF) and aqueous soluble fractions (ASF) were subjected to different chromatographic techniques to isolate secondary metabolites. Successive chromatographic separation and purification yielded a total of two compounds identified and characterized as Taraxerone(1) and 5,8-dihydroxy- 6-methoxy-4,9-dioxo-1,3,4,9-tetrahydronaphthol[2,3-c]furan-1-yl acetate (2) by extensive proton NMR spectrum (1H-NMRspectrum) analysis. The different partitionate like PESF, EASF, MESF and ASF were subjected to screen their antimicrobial properties against some selected Gram positive and Gram negative bacteria and fungi, brine shrimp lethality and antioxidant activities. The maximum zone of inhibition of chloroform extract was found against Pseudomonas sp. (16mm). All fractions showed more activity against Gram negative bacteria then Gram positive bacteria. In the brine shrimp lethality bioassay, among all extracts, the petroleum ether and ethyl acetate soluble fraction showed significant lethality having the LC50 value of 7.72 μg/ml. The antioxidant activity was evaluated in terms of determination of free radical scavenging activity (DPPH assay). Among all the extracts of S. apetala the highest free radical scavenging activity showed by (Methanol soluble fraction) MESF with IC50 value 18.0 μg/ml. Bangladesh J Microbiol, Volume 38, Number 1, June 2021, pp 1-5

scholarly journals Rapid Screening and Structural Characterization of Antioxidants from the Extract ofSelaginella doederleiniiHieron with DPPH-UPLC-Q-TOF/MS Method

2015 ◽  
Vol 2015 ◽  
pp. 1-9 ◽  
Author(s):  
Gang Wang ◽  
Shun Yao ◽  
Xiu-Xiu Zhang ◽  
Hang Song
Keyword(s):  
Mass Spectrometry ◽  
High Performance ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Rapid Screening ◽  
Flight Mass Spectrometry ◽  
First Time ◽  
Strong Capacity ◽  
Tof Ms

2,2-Diphenyl-1-picrylhydrazyl-ultra-high performance liquid chromatography-Q-time-of-flight mass spectrometry (DPPH-UPLC-Q-TOF/MS), as a rapid and efficient means, now was used for the first time to screen antioxidants fromSelaginella doederleinii. The nine biflavone compounds were screened as potential antioxidants. The biflavones were structurally identified and divided into the three types, that is, amentoflavone-type, robustaflavone-type, and hinokiflavone-type biflavonoids. Among the compounds bilobetin (3) and putraflavone (8) were found fromSelaginella doederleiniifor the first time and others including amentoflavone (1), robustaflavone (2), 4′-methoxy robustaflavone (4), podocarpusflavone A (5), hinokiflavone (6), ginkgetin (7), and heveaflavone (9) were identified previously in the plant. Moreover, nine biflavones possessed a good antioxidant activity via their DPPH free radical scavenging. It demonstrates that DPPH-UPLC-Q-TOF/MS exhibits strong capacity in separation and identification for small molecule. The method is suitable for rapid screening of antioxidants without the need for complicated systems and additional instruments.

scholarly journals Phytochemicals Screening and Chromatographic Separation of Bio Active Compound from the Roots of Berberis Lyceum

2019 ◽  
pp. 1-5
Author(s):  
Shakir Ullah ◽  
◽  
Inam Ullah ◽  
Robeena Naz ◽  
◽  
...  
Keyword(s):  
Chromatographic Separation ◽  
Research Work ◽  
Ethanol Extract ◽  
Spectroscopic Methods ◽  
H Nmr ◽  
Phytochemical Investigation ◽  
Isolated Compound ◽  
First Time ◽  
Berberis Lycium

In the present research work the phytochemical and Chromatographic separation of bio active compounds Berberis lycium occurred. Phytochemical investigation of the CH2 Cl2 /CH3 OH (1:1) extract ethanol of root shown the presence of terpenoids, flavonoids, saponins, anthraquinones and alkaloids, steroids and tannins was absent. Chromatographic separation of CH2 Cl2 /CH3 OH (1:1) root ethanol extract yielded methyl 9, 10-dihydro-3, 8-hydroxy-6-methyl-9, 10-dioxoanthracene-2- carboxylate, isolated for the first time from the species of Berberis lycium. With the help of spectroscopic methods (IR, 1 H NMR, 13C NMR, and DEPT-135 and UV-Vis,) Partial characterization of the isolated compound was done.

A New Chlorine-containing Glucosyl-fused Compound from Curculigo glabrescens

2009 ◽  
Vol 64 (9) ◽  
pp. 1077-1080 ◽  
Author(s):  
Cui-Cui Zhu ◽  
Tian-Ming Wang ◽  
Kai-Jin Wang ◽  
Ning Li
Keyword(s):  
Spectroscopic Analysis ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
2D Nmr ◽  
Free Radical Scavenging ◽  
Scavenging Activity ◽  
Dpph Assay ◽  
Etoh Extract ◽  
Structure Activity ◽  
First Time

A new chlorine-containing glucosyl-fused compound, crassifoside H (1), was isolated from the EtOH extract of the rhizomes of Curculigo glabrescens. The structure was established on the basis of MS, IR, 1D and 2D NMR experiments. In addition, seven known compounds (2 - 8) were isolated and identified by spectroscopic analysis and comparison of their spectral data with those reported previously. All the compounds were isolated from this plant for the first time. The free radical scavenging activity of the isolated compounds was evaluated by the 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay. Compounds 1 and 2 showed strong radical scavenging activities. The primary structure-activity relationship is also discussed.

scholarly journals A Mini Library of Novel Triazolothiadiazepinylindole Analogues: Synthesis, Antioxidant and Antimicrobial Evaluations

2014 ◽  
Vol 2014 ◽  
pp. 1-10 ◽  
Author(s):  
Jaiprakash Sharanappa Biradar ◽  
Parveen Rajesab ◽  
Naveen Jaiprakash Biradar ◽  
Sasidhar Balappa Somappa
Keyword(s):  
Radical Scavenging Activity ◽  
Analytical Data ◽  
Radical Scavenging ◽  
Antimicrobial Activities ◽  
Free Radical Scavenging ◽  
Scavenging Activity ◽  
H Nmr ◽  
Total Antioxidant ◽  
Antioxidant And Antimicrobial Activities ◽  
Antimicrobial Potency

A new series of novel triazolothiadiazepinylindole analogues were synthesized with an aim to examine possible antioxidant and antimicrobial activities. The titled compounds (3a–z) were obtained in good yield by reacting 5-(5-substituted-3-phenyl-1H-indol-2-yl)-4-amino-4H-1,2,4-triazole-3-thiols1a–cwith 3-(2,5-disubstituted-1H-indol-3-yl)-1(4-substituted phenyl)prop-2-en-1-ones2a–i. All the newly synthesized compounds were characterized by IR,1H NMR, mass spectroscopic and analytical data. The synthesized analogues were tested for antioxidant and antimicrobial potency. Among the tested compounds3a–cand3j–lhave shown very promising free radical scavenging activity and total antioxidant capacity. Compounds3d–f,3m–o, and3s–zhave shown excellent ferric reducing antioxidant activity. An outstanding antimicrobial activity is observed with compounds3a–cand3j–l.

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