scholarly journals Glial Wingless/Wnt Regulates Glutamate Receptor Clustering and Synaptic Physiology at the Drosophila Neuromuscular Junction

2014 ◽  
Vol 34 (8) ◽  
pp. 2910-2920 ◽  
Author(s):  
K. S. Kerr ◽  
Y. Fuentes-Medel ◽  
C. Brewer ◽  
R. Barria ◽  
J. Ashley ◽  
...  
Keyword(s):  
Neuromuscular Junction ◽  
Glutamate Receptor ◽  
Receptor Clustering ◽  
Synaptic Physiology

scholarly journals Lola regulates glutamate receptor expression at the Drosophila neuromuscular junction

Biology Open ◽  
2012 ◽  
Vol 1 (4) ◽  
pp. 362-375 ◽  
Author(s):  
A. Fukui ◽  
M. Inaki ◽  
G. Tonoe ◽  
H. Hamatani ◽  
M. Homma ◽  
...  
Keyword(s):  
Neuromuscular Junction ◽  
Glutamate Receptor ◽  
Receptor Expression

scholarly journals Nuclear Factor  B Controls Acetylcholine Receptor Clustering at the Neuromuscular Junction

2010 ◽  
Vol 30 (33) ◽  
pp. 11104-11113 ◽  
Author(s):  
J. Wang ◽  
X.-Q. Fu ◽  
W.-L. Lei ◽  
T. Wang ◽  
A.-L. Sheng ◽  
...  
Keyword(s):  
Neuromuscular Junction ◽  
Acetylcholine Receptor ◽  
Nuclear Factor ◽  
Receptor Clustering ◽  
Factor B

scholarly journals Synaptic homeostasis at the Drosophila neuromuscular junction is a reversible signaling process that is sensitive to high temperature

2017 ◽  
Author(s):  
Catherine J. Yeates ◽  
C. Andrew Frank
Keyword(s):  
Drosophila Melanogaster ◽  
Neuromuscular Junction ◽  
Glutamate Receptor ◽  
Physiological Activity ◽  
Expression System ◽  
Self Regulation ◽  
Neuromuscular Synapse ◽  
Dominant Negative ◽  
Set Point ◽  
Corrective Measures

ABSTRACTHomeostasis is a vital mode of biological self-regulation. The hallmarks of homeostasis for any biological system are a baseline set point of physiological activity, detection of unacceptable deviations from the set point, and effective corrective measures to counteract deviations. Homeostatic synaptic plasticity (HSP) is a form of neuroplasticity in which neurons and circuits resist environmental perturbations in order to maintain appropriate levels of activity. One assumption is that if an environmental perturbation triggers homeostatic corrective changes in neuronal properties, those corrective measures should be reversed upon removal of the perturbation. We test the reversibility and limits of HSP at a well-studied model synapse, the Drosophila melanogaster neuromuscular junction (NMJ). At the Drosophila NMJ, impairment of glutamate receptors causes a decrease in quantal size, which is offset by a corrective, homeostatic increase in the number of vesicles released per evoked presynaptic stimulus, or quantal content. This process has been termed presynaptic homeostatic potentiation (PHP). Taking advantage of a GAL4/GAL80TS/UAS expression system, we triggered PHP by expressing a dominant-negative glutamate receptor subunit at the NMJ. We then reversed PHP by halting expression of the dominant-negative receptor. Our data show that PHP is fully reversible over a time course of 48-72 hours after the dominant-negative glutamate receptor stops being genetically expressed. Additionally, we found that the PHP response triggered by the dominant-negative subunit was ablated at high temperatures. Our data show that the long-term maintenance of PHP at the Drosophila NMJ is a reversible regulatory process that is sensitive to temperature.SIGNIFICANCE STATEMENTBiological homeostatic systems must upregulate or downregulate cellular parameters in order to maintain appropriate set points of physiological activity. Homeostasis is a well-documented mode of regulation in metazoan nervous systems. True homeostatic control should be a reversible process – but due to technical difficulties of presenting and removing functional challenges to living synapses, the reversibility of homeostatic forms of synapse regulation has not been rigorously examined in vivo over extended periods of developmental time. Here we formally demonstrate that homeostatic regulation of Drosophila melanogaster neuromuscular synapse function is reversible and temperature-labile. This is significant because developing methods to study how homeostatic regulatory systems are turned on and off could lead to fundamental new insights about control of synaptic output.

scholarly journals Nanoscale co-organization and coactivation of AMPAR, NMDAR, and mGluR at excitatory synapses

2020 ◽  
Vol 117 (25) ◽  
pp. 14503-14511 ◽  
Author(s):  
Julia Goncalves ◽  
Tomas M. Bartol ◽  
Côme Camus ◽  
Florian Levet ◽  
Ana Paula Menegolla ◽  
...  
Keyword(s):  
Glutamate Receptor ◽  
Single Molecule ◽  
Glutamate Release ◽  
Neurotransmitter Receptors ◽  
Excitatory Synapses ◽  
Superresolution Microscopy ◽  
Synaptic Physiology ◽  
Transmission Properties ◽  
Presynaptic Release ◽  
Average Quantity

The nanoscale co-organization of neurotransmitter receptors facing presynaptic release sites is a fundamental determinant of their coactivation and of synaptic physiology. At excitatory synapses, how endogenous AMPARs, NMDARs, and mGluRs are co-organized inside the synapse and their respective activation during glutamate release are still unclear. Combining single-molecule superresolution microscopy, electrophysiology, and modeling, we determined the average quantity of each glutamate receptor type, their nanoscale organization, and their respective activation. We observed that NMDARs form a unique cluster mainly at the center of the PSD, while AMPARs segregate in clusters surrounding the NMDARs. mGluR5 presents a different organization and is homogenously dispersed at the synaptic surface. From these results, we build a model predicting the synaptic transmission properties of a unitary synapse, allowing better understanding of synaptic physiology.

scholarly journals Calcium-Activated Calpain Specifically Cleaves Glutamate Receptor IIA But Not IIB at the Drosophila Neuromuscular Junction

2019 ◽  
Vol 39 (15) ◽  
pp. 2776-2791 ◽  
Author(s):  
Elsayed Metwally ◽  
Guoli Zhao ◽  
Wenhua Li ◽  
Qifu Wang ◽  
Yong Q. Zhang
Keyword(s):  
Neuromuscular Junction ◽  
Glutamate Receptor

scholarly journals Wnt/β-Catenin Signaling Suppresses Rapsyn Expression and Inhibits Acetylcholine Receptor Clustering at the Neuromuscular Junction

2008 ◽  
Vol 283 (31) ◽  
pp. 21668-21675 ◽  
Author(s):  
Jia Wang ◽  
Nan-Jie Ruan ◽  
Lei Qian ◽  
Wen-liang Lei ◽  
Fei Chen ◽  
...  
Keyword(s):  
Neuromuscular Junction ◽  
Acetylcholine Receptor ◽  
Receptor Clustering
Sign in / Sign up

Export Citation Format

Share Document