scholarly journals Seed vigour evaluation in petunia seed lots to predict seedling emergence and longevity

2020 ◽  
Vol 48 (3) ◽  
pp. 391-400
Author(s):  
Ibrahim Demir ◽  
Nihal Erturk ◽  
Zeynep Gokdas
Keyword(s):  
Electrical Conductivity ◽  
Seedling Emergence ◽  
Accelerated Ageing ◽  
Seed Vigour ◽  
Germination Time ◽  
Ageing Test ◽  
Radicle Emergence ◽  
Mean Germination Time ◽  
Storage Potential ◽  
And Storage

This study examined the potential of different vigour tests to reveal differences in performance of nine hybrid petunia seed lots. Saturated salt accelerated ageing test (SSAA) and 48-hour radicle emergence (RE48h) tests were able to predict seedling emergence and longevity potential. The results of accelerated ageing (AA) (at 41/43°C, 48/72 hours, 100% RH), saturated salt accelerated ageing (SSAA) (at 41/43/45°C, 72 hours, 75% RH), mean germination time (MGT), 48-hour radicle emergence (RE48h) and electrical conductivity (EC) tests were compared with seedling emergence in modules and longevity (at 5°C for 24 months and 25°C for 12 months). Initial standard germination (SG) percentages were above 83% in the lots. Seedling emergence ranged between 18 and 89%, germination after storage was between 16 and 88% at 5°C, and 13 and 96% at 25°C. The SSAA test at 43°C and 45°C for 72 hours was correlated with both seedling emergence and longevity. RE48h was correlated with emergence and germination after storage at 5°C for 24 months; MGT and SG was correlated with seedling emergence. Neither AA nor the EC test was correlated with emergence and longevity. Thus vigour, expressed as both emergence and storage potential, was successfully predicted by the SSAA test and RE48h.

scholarly journals Seed Vigour Tests to Estimate Seedling Emergence in Cress (Lepidium sativum L.) Seed Lots

2019 ◽  
Vol 47 (3) ◽  
Author(s):  
Ibrahim DEMIR ◽  
Burcu B. KENANOGLU ◽  
Eren ÖZDEN
Keyword(s):  
Electrical Conductivity ◽  
Seedling Emergence ◽  
Accelerated Aging ◽  
Lepidium Sativum ◽  
Accelerated Ageing ◽  
Digital Object Identifier ◽  
Seed Vigour ◽  
Significance Levels ◽  
Radicle Emergence ◽  
Lepidium Sativum L

This work was carried out to estimate field and controlled room seedling emergence potential through seed vigour tests in cress (Lepidium sativum L.) seeds. Early radicle emergence percentages after 12 (RE12h), 24 (RE24h) and 36 (RE36h) hours of germination test, mean germination time, accelerated aging (45 °C, 100% RH, 24 h), electrical conductivity (EC) of soaking water (40 ml, 50 seeds, 20 °C), after 16 hours (EC16h) and 24 hours (EC24h), and EC16h and EC24h after accelerated ageing (AA, 45 °C, 100% RH, 24h) were tested as vigour tests in ten commercial seed lots of cress. Standard laboratory germination ranged between 88 and 93%. Seeds were sown on two occasions in field and controlled room conditions, and seedling emergence percentages were determined after 30 days in the soil and 14 days in the controlled room. Seedling emergence ranged between 67 and 85% and 59 and 83% in the first and second sowings in the field. These values were 75 and 92% in controlled room sowing. Vigour tests were correlated to seedling emergence potential at various significance levels but RE24h and EC16h showed the highest correlation coefficient values (p < 0.001) in all three sowing conditions as r = 0.879-0.988 in RE24h, and r = 0.902-0.962 in EC16h. Results indicated that early radicle emergence percentages after 24 hours (RE24h) and electrical conductivity value after 16 hours (EC16h) can be successfully used to estimate the seedling emergence potential of cress seeds in field and controlled room conditions.   ********* In press - Online First. Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (Volume 47, Issue 3, 2019). The article is searchable and citable by Digital Object Identifier (DOI). DOI link will become active after the article will be included in the complete issue. *********

Prediction of Field Performance of Seed Lots of Groundnut (Arachis hypogaea L.) through Vigour Tests

2021 ◽  
Author(s):  
N.K. Biradarpatil ◽  
Smayli Rana ◽  
Shivasharanappa S. Patil
Keyword(s):  
Electrical Conductivity ◽  
Field Performance ◽  
Plant Population ◽  
Accelerated Ageing ◽  
Natural Ecosystem ◽  
Seed Vigour ◽  
Germination Time ◽  
Pod Yield ◽  
Mean Germination Time ◽  
Highly Correlated

Background: Profitable farming indeed needs a seed which emerges and performs better under natural ecosystem. The inadequacy of seed germination test in predicting the field performance of a seed lot, as it is carried out under controlled laboratory conditions which may not meet in the field necessitates the development and standardization of tests in a way that would reflect the performance of seeds in the field through laboratory tests.Methods: A laboratory based seed vigour tests were undertaken with 20 different seed lots of groundnut variety GPBD-4 having different vigour levels to predict the field performance. The correlation and regression analysis among the laboratory based seed vigour tests and field performance was analyzed.Conclusion: The significance of lab vigour test on the field performance revealed that, electrical conductivity was highly negatively correlated to seed emergence, speed of emergence, plant population and pod yield with r = - 0.885, - 0.885, -0.843 and -0.845, respectively. Similarly, accelerated ageing was highly correlated to plant population (r = 0.864), plant height (0.737) and pod yield (r = 0.815). Whereas, the mean germination time calculated based on radicle emergence test contributed more than 80% to field performances and mean while, electrical conductivity influencing for 71.4% variation in the pod yield. Thus, hinting refinement and use of electric conductance, accelerated ageing and mean germination time tests towards efficient prediction of field performance of groundnut seed lots.

scholarly journals Potential of a single radicle emergence count in predicting field emergence of Desi chickpea seed lots as an alternative vigour test

2019 ◽  
Author(s):  
Maliheh Akbarpour ◽  
Mohammad Khajeh-Hosseini ◽  
Alireza Seifi
Keyword(s):  
Electrical Conductivity ◽  
Coefficient Of Determination ◽  
Time Intervals ◽  
Crop Species ◽  
Seed Vigour ◽  
Germination Time ◽  
Regular Time ◽  
Radicle Emergence ◽  
Mean Germination Time ◽  
Field Emergence

Single early counts of radicle emergence (RE) is a validated vigour test for four crop species. We performed this study to see whether an early radicle emergence (RE) count predicts the emergence of seedlings in Desi chickpea seed lots as well as an electrical conductivity (EC) test. Electrical conductivity measurements of 11 seed lots were highly predictive of field emergence (FE) (R2 = 0.626**). The radicle emergence was counted at regular time intervals of six hours, up to 72 hours after imbibition. Mean germination time (MGT) clearly predicted variation in number of seedlings in the field. This evidence supports the idea that the variation in the germination time between seed lots can be an early indicator of seed vigour. Early RE counts at 48 hours also had a high coefficient of determination (R2 = 0.764***) with FE. The negative correlation between EC and the single early count of RE at different germination times indicated that the two tests identified the same differences in vigour. The strong relationships between RE at 48 hours with both EC and FE supports the proposal that a single count of RE could be an alternative vigour test for Desi chickpea.

scholarly journals Electrical conductivity and mineral composition of the imbibition solution of bean seeds during storage

2012 ◽  
Vol 36 (2) ◽  
pp. 147-155 ◽  
Author(s):  
Claudia Denise da Silva ◽  
Mariana Silva Rosa Pazeto ◽  
Roberval Daiton Vieira
Keyword(s):  
Electrical Conductivity ◽  
Mineral Composition ◽  
Storage Period ◽  
Seed Storage ◽  
Effect Of Temperature ◽  
Accelerated Ageing ◽  
Bean Seed ◽  
Seed Vigour ◽  
Calcium Magnesium ◽  
And Storage

The electrical conductivity test has been used to evaluate seed vigour in many species, especially legumes, such as peas, beans and soybeans. The objective of this research was to evaluate the effect of temperature and storage period on the results of the electrical conductivity test and the mineral composition of the imbibition solution of bean seeds (Phaseolus vulgaris L.). Three seed lots of the cultivar IAPAR - 81 and cultivar IPR - TIZIU were stored at 10, 25 and 25-10º C (six months at 10º C and six months at 25º C) The following evaluations were made every three months for a year: seed water content, germination and vigour (accelerated ageing and electrical conductivity) and the potassium, calcium and magnesium contents of the imbibition solution. The cultivars showed similar behavior in storage. The reduction in the vigour of bean seeds stored at 10º C was verified by accelerated ageing and electrical conductivity tests. The bean seed storage at low temperature (10º C) does not influence the results of the evaluation of seed vigour by electrical conductivity test and the quantification of calcium, magnesium and potassium ions. The evaluation of vigour by the electrical conductivity test is not recommended for bean seed stored by long periods (above 9 months).

scholarly journals Radicle emergence test as a quick vigour test to predict field emergence performance in rice (Oryza sativa L.) seed lots

2021 ◽  
Vol 13 (SI) ◽  
pp. 86-93
Author(s):  
Chinnasamy G P ◽  
Sundareswaran S ◽  
Renganayaki P R ◽  
Vetrivel M
Keyword(s):  
Oryza Sativa L ◽  
Rice Seed ◽  
Quick Method ◽  
Seed Vigour ◽  
Germination Time ◽  
Seed Industry ◽  
Radicle Emergence ◽  
Emergence Percentage ◽  
Mean Germination Time ◽  
Field Emergence

An experiment was made to standardize the radicle emergence test to predict the field emergence performance in ten different seed lots [L1 to L4: high vigour lots (> 90 % germination), L5 to L7: medium vigour lots (80-90 % germination) and L8 to L10: low vigour lots (< 80 % germination)] of rice cv. CO 51. The results showed that the significant differences are observed in physiological and biochemical parameters in different seed lots. The seed vigour was classified into three groups viz., high, medium and low vigour based on the relationship between mean germination time and field emergence. When the Mean Germination Time (MGT) was < 34 hours, the field emergence was > 85 per cent, which was considered as high vigour; when the MGT was 34-35 hours, the field emergence was 80-85 per cent, that was considered as medium vigour; when the MGT was > 35 hours, the field emergence was < 80 per cent, that was considered as low vigour. The radicle emergence test (2mm radicle length) was highly negatively correlated with mean germination time (-0.930**) followed by mean just germination time (-0.852**) and electrical conductivity of seed leachate (-0.827**) and it was positively correlated with field emergence (0.894**) followed by germination (0.878**) and dehydrogenase activity (0.864**). The R2 values between seed vigour parameters and radicle emergence test were significantly higher in 2mm length of radicle emergence when compared with 1mm length of radicle emergence. Finally, the study concluded that 36 hour MGT with the attainment of 2mm radicle emergence percentage could be used as a quick method to assess rice seed lots' quality by the seed analysts and seed industry.

scholarly journals Evaluation of Seed Quality Attributes of Sorghum Germplasm Accessions from Eastern, Coastal and Nyanza Regions, Kenya

2020 ◽  
Vol 9 (3) ◽  
pp. 9
Author(s):  
C. W. Muui ◽  
R. M. Muasya ◽  
S. Nguluu ◽  
A. Kambura
Keyword(s):  
Food Security ◽  
Seed Quality ◽  
Germination Percentage ◽  
Seed Vigour ◽  
Subsistence Farmers ◽  
Germination Time ◽  
Vigour Index ◽  
Mean Germination Time ◽  
Handling Practices ◽  
Germplasm Accessions

Sorghum (Sorghum bicolor L.) is an important cereal crop in Kenya. Despite the crops importance, the yields attained by farmers in Eastern, Coastal and Nyanza regions of Kenya remain low. Access to good quality seeds of sorghum is one of the constraints facing the subsistence farmers. Good quality seed is important for increasing yield to attain food security. The aim of this study was to evaluate quality attributes of the seed used by farmers. A total of 108 germplasm accessions were obtained from 76 farmers. The seeds were tested for time and percentage of germination, seed vigour index, shoot and root dry weight. Data collected was subjected to analysis of variance. Means were separated using Fisher&rsquo;s Least Significance Difference test at p &le; 0.05. Seed samples of 26 accessions attained germination percentage below stipulated standards by Seeds and Plant Varieties Act CAP 326. Majority of seeds showed longer mean germination time with only nine accessions germinating in less than ten days. Seed vigour index was relatively high in most of the accessions, while biomass accumulation varied from high to very low among accessions. Though most of the seeds attained a high germination percentage, about 92% of seeds showed longer mean germination time. The environmental conditions in the fields, pre and post harvest handling practices impact on the seed quality hence the wide variability in germination percentage, germination time, seed vigour index and dry matter accumulation. Therefore the need to improve quality of seeds used by subsistence farmers by providing extension services on best pre and post harvest handling practices. Increasing production of sorghum in these regions will contribute significantly towards realizing food security. Further analysis could be carried out on genetic and sanitary quality aspects of the seeds planted by farmers in Eastern, Nyanza and Coastal regions.

scholarly journals Physiological potential of eggplant seeds

2013 ◽  
Vol 35 (2) ◽  
pp. 225-230 ◽  
Author(s):  
Magnólia de Mendonça Lopes ◽  
Clíssia Barboza da Silva ◽  
Roberval Daiton Vieira
Keyword(s):  
Electrical Conductivity ◽  
Seed Germination ◽  
Moisture Content ◽  
Seedling Emergence ◽  
Solanum Melongena ◽  
The Other ◽  
Accelerated Ageing ◽  
Preliminary Information ◽  
H 41 ◽  
Controlled Deterioration

The use and efficiency of vigor tests depend on the plant species and the development and, or adjustment of methodology, to obtain efficient procedures for evaluating seed physiological potential. The objective of this study was to compare different methods for evaluating the physiological potential of eggplant seeds (Solanum melongena L). Six seed lots of the "Comprida Roxa" cultivar were submitted to the following tests: germination, first count germination, seedling emergence, controlled deterioration (18, 21 and 24% for 24 and 48 hours at 45 °C), accelerated ageing (AA) and saturated salt accelerated ageing (both at 41 and 45 °C for 24, 48 and 72 hours) and electrical conductivity (25 seeds, 50 and 75 mL of water for 1, 2, 4, 8, 12, 16 and 24 hours of imbibition at 25 °C). The first count test provided preliminary information on seed germination and the other tests, in general, gave similar results for seedling emergence. It was concluded that the physiological potential of eggplant seeds can be evaluated using AA for 48 h/41 °C and controlled deterioration at 24% moisture content/ 24 h/45 °C. The best conditions for the electrical conductivity test on eggplant seed are using 25 seeds in 50mL of water for four hours at 25 ºC.

Environmental factors affecting the germination and emergence of white horehound (Marrubium vulgare L.): a weed of arid-zone areas

2018 ◽  
Vol 40 (1) ◽  
pp. 47 ◽  
Author(s):  
Muhammad M. Javaid ◽  
Singarayer K. Florentine ◽  
Hafiz H. Ali ◽  
Bhagirath S. Chauhan
Keyword(s):  
Osmotic Potential ◽  
Seedling Emergence ◽  
Alkaline Media ◽  
Germination Index ◽  
Arid Zones ◽  
Burial Depth ◽  
Germination Time ◽  
Wide Range ◽  
Marrubium Vulgare ◽  
Mean Germination Time

White horehound (Marrubium vulgare L.), is a troublesome weed of arid zones, particularly in cropping and grazing areas. Understanding the seed ecology of white horehound is relevant to its management. The present study investigated the effects of temperature, light, osmotic potential, salinity, pH, and seeding depth on seed germination and seedling emergence of white horehound. Seeds germinated over a wide range of temperatures, with highest germination (100%) occurring at 25/20°C in either 12-h alternating light/dark or complete darkness. Germination decreased from 92% to 10% as the osmotic potential decreased from 0 to minus 0.6 MPa and germination was completely inhibited at minus 0.8 MPa. Increasing concentration of sodium chloride (salinity) from 50 to 150 mM reduced germination from 68% to 11% but was completely inhibited at 200 mM. Germination was not limited by variation of pH in solutions of pH 5 to pH 10. Maximum germination (99%) occurred at pH 7, but slightly decreased (90%) in acidic or alkaline media. Surface located seeds were highly germinable (87%) where seedling emergence significantly declined as seeding depth increased from 0.5 to 3 cm. Nil seedling emergence was occurred at 4 cm burial depth. Light significantly affected germination, time to 50% germination, mean germination time and germination index. Increasing osmotic and salinity stress both significantly increased the time to start germination, time to 50% germination, mean germination time, but decreased germination index. Burial depth adversely affected seedling emergence with surface located seedlings emerging earlier (4.2 days) compared with 20 days for seeds buried at 3 cm. Results suggest that effective management of white horehound should consider targeting surface located seeds in combination with tools that induce minimal soil disturbance, particularly in relatively non-saline environments.

scholarly journals Effect of hydropriming method on maize (Zea mays) seedling emergence

2014 ◽  
Vol 39 (1) ◽  
pp. 143-150 ◽  
Author(s):  
KU Ahammad ◽  
MM Rahman ◽  
MR Ali
Keyword(s):  
Seedling Emergence ◽  
Research Work ◽  
Germination Percentage ◽  
Maize Seedling ◽  
Germination Index ◽  
Percentage Germination ◽  
Saturated Sand ◽  
Germination Time ◽  
Mean Germination Time ◽  
Surface Drying

The research work was carried out at the Seed Laboratory of Agronomy Department of Bangladesh Agricultural University, Mymensingh during the period from February to April in 2008 to find out the effect of hydropriming methods on maize seedling emergence. The hydropriming methods were- T1 (Non-priming), T2 (14 hours soaking + drying + storing), T3 (18 hours soaking + drying + storing), T4 (22 hours soaking + drying + storing), T5 (14 hours soaking + surface drying), T6 (18 hours soaking + surface drying), and T7 (22 hours soaking + surface drying). Effect of different hydropriming methods on seedling emergence performance of maize was evaluated at two moisture levels viz., 30% and 60% moisture of saturated sand in the experiment. Germination percentage, germination index and mean germination time were influenced significantly by hydropriming methods. The highest germination percentage, germination index, and lowest mean germination time were recorded with T6 (18 hours soaking + surface drying). DOI: http://dx.doi.org/10.3329/bjar.v39i1.20164 Bangladesh J. Agril. Res. 39(1): 143-150, March 2014

scholarly journals Seed germination and seedling growth of two Pseudobombax species (Malvaceae) with contrasting habitats from Brazilian Cerrado

2018 ◽  
pp. 1915-1925
Author(s):  
Clesnan Mendes-Rodrigues ◽  
Paulo Eugênio Oliveira ◽  
Marli Aparecida Ranal
Keyword(s):  
Seed Germination ◽  
Seedling Growth ◽  
Underground Structure ◽  
Seedling Emergence ◽  
Dry Mass ◽  
Underground Structures ◽  
Germination Time ◽  
Central Brazil ◽  
Mean Germination Time ◽  
Contrasting Habitats

Pseudobombax tomentosum and P. longiflorum are common trees in the Cerrado region, but the former species is more common in forest edges while the later is present in open cerrado areas. This work aimed to investigate differences in seed germination and seedling growth in these species, from seed collected from Cerrado areas in Central Brazil. For this, a seed germination experiment was designed and included four replicates with 25 seeds per species; seeds were randomly distributed in the germination chamber. To evaluate initial seedling growth, seedlings height was measured up to 67 days after seedling emergence; besides, some of these seedlings were grown for biomass evaluation during nine months. Results showed that seeds of the two species had the same germinability (near 100%) and mean germination time (ca. 12 days). However, P. longiflorum showed a more spread seed germination through time, with higher values of coefficient of variation in germination time and uncertainty index; and lower values of synchronization than P. tomentosum. The two species showed basically the same growth pattern, but lower values for height of apical meristem, diameter of underground structures (mostly roots), dry mass of shoots, underground structure and total mass of seedlings in P. tomentosum were obtained, compared to P. longiflorum. Both species allocated more dry mass to underground structures in detriment of shoot. This probably allows resprouting behavior which prevents hydric stress and detrimental fire action typical of the open Cerrado areas.

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