FABP 4 is associated with inflammatory markers and metabolic syndrome in morbidly obese women

ObjectiveThe adipocyte/macrophage fatty acid-binding protein 4 (FABP4) has been described as a biomarker for adiposity and metabolic syndrome (MS). The aims of this study were to assess the relationship between FABP4 and inflammatory cytokines related to obesity, and to evaluate FABP4 mRNA expression in visceral and subcutaneous adipose tissue in non-diabetic morbidly obese women versus healthy lean women.MethodsWe analyzed circulating levels of FABP4 in 81 Spanish women: 38 lean (body mass index (BMI)<25 kg/m2) and 43 morbidly obese (BMI>40 kg/m2). We took 30 follow-up blood samples at 6 and 12 months after bariatric surgery. We assessed FABP4 gene expression in samples of subcutaneous abdominal and visceral adipose tissue. Adipose tissue mRNA expression was determined by real-time RT-PCR.ResultsIn morbidly obese women, plasma FABP4 levels were significantly higher than in non-obese patients. These levels positively correlated with BMI, homeostasis model assessment of insulin resistance (HOMA2-IR), and plasma glucose and insulin levels. Post-operative FABP4 levels decreased by a maximum of 30% after 12 months. We also found an inverse association between FABP4 and adiponectin levels, and positive correlations between FABP4 and circulating leptin, tumor necrosis factor (TNF) receptors, C-reactive protein (CRP) and interleukin 6 levels. Linear regression analysis revealed that FABP4 was more closely related to HOMA2-IR than adiponectin, CRP, TNF-RI, or leptin. Furthermore, high circulating FABP4 levels were associated with the presence of MS. FABP4 mRNA expression in visceral adipose tissue was related to its circulating levels in morbidly obese women.ConclusionsOur results indicate that serum FABP4 is associated with inflammatory factors related to obesity and MS in non-diabetic morbidly obese women.

Download Full-text

Resistin Gene Expression in Visceral Adipose Tissue of Postmenopausal Women and its Association with Insulin Resistance

Women s Health ◽

10.2217/whe.12.46 ◽

2012 ◽

Vol 8 (5) ◽

pp. 521-528 ◽

Cited By ~ 7

Author(s):

Sadashiv ◽

Sunita Tiwari ◽

Bhola Nath Paul ◽

Sandeep Kumar ◽

Abhijit Chandra ◽

...

Keyword(s):

Insulin Resistance ◽

Adipose Tissue ◽

Mrna Expression ◽

Postmenopausal Women ◽

Visceral Adipose Tissue ◽

Model Assessment ◽

Inverse Association ◽

Obese Women ◽

Visceral Adipose ◽

Resistin Mrna

Aim: The present study evaluates resistin mRNA expression in visceral adipose tissue (VAT) and its correlation with insulin resistance (homeostatic model assessment) in postmenopausal obese women. Materials & methods: A total of 68 (nonobese = 34 and obese = 34) age-matched (49–70 years) postmenopausal women were recruited for the study. Fasting blood samples were collected at admission and abdominal VAT were obtained during surgery for gall bladder stones or hysterectomy. Physical parameters (age, height, weight and BMI) were measured. Biochemical parameters (plasma insulin, plasma glucose and serum resistin) were estimated by enzymatic methods. The VAT resistin mRNA expression was evaluated by real-time PCR. Results: The relative mean (± standard deviation) VAT resistin mRNA expression in postmenopausal obese women lowered significantly by 20.4% compared with postmenopausal nonobese women (0.029 ± 0.011 vs 0.023 ± 0.013; p = 0.047). Furthermore, VAT resistin mRNA expression in postmenopausal obese women was downregulated by 0.69-fold when compared with age-matched postmenopausal nonobese women. Furthermore, the relative VAT resistin mRNA expression in postmenopausal obese women showed significant inverse association with insulin resistance (r = −0.48; p < 0.01) and serum resistin (r = −0.84; p < 0.001), while in postmenopausal nonobese women it did not show any association with both insulin resistance (r = 0.03; p > 0.05) and serum resistin (r = −0.03; p > 0.05). Conclusion: The VAT resistin mRNA expression in postmenopausal obese women is associated to insulin resistance.

Download Full-text

Association Between Visceral Adipose Tissue and Estradiol with Bone Health among Obese Women with Metabolic Syndrome

Pakistan Journal of Biological Sciences ◽

10.3923/pjbs.2020.1237.1244 ◽

2020 ◽

Vol 23 (10) ◽

pp. 1237-1244

Author(s):

Nayera E. Hassan ◽

Sahar A. El-Masr ◽

Gamila S.M. El-Sa ◽

Muhammad Al-Tohamy ◽

Eman H. Thabet ◽

...

Keyword(s):

Metabolic Syndrome ◽

Adipose Tissue ◽

Visceral Adipose Tissue ◽

Bone Health ◽

Obese Women ◽

Visceral Adipose

Download Full-text

Tadalafil ameliorates metabolic syndrome-induced alterations in visceral adipose tissue and liver: an experimental study in the rabbit

Endocrine Abstracts ◽

10.1530/endoabs.35.oc9.2 ◽

2014 ◽

Author(s):

Linda Vignozzi ◽

Sandra Filippi ◽

Ilaria Cellai ◽

Paolo Comeglio ◽

Elena Maneschi ◽

...

Keyword(s):

Metabolic Syndrome ◽

Experimental Study ◽

Adipose Tissue ◽

Visceral Adipose Tissue ◽

Visceral Adipose

Download Full-text

Correction of morphofunctional disorders with grapes polyphenols in rats with experimental metabolic syndrome

Nauchno-prakticheskii zhurnal «Patogenez» ◽

10.25557/gm.2018.4.9748 ◽

2018 ◽

pp. 43-48

Author(s):

Ю.И. Шрамко ◽

А.В. Кубышкин ◽

А.А. Давыдова ◽

И.И. Фомочкина ◽

Л.Л. Алиев ◽

...

Keyword(s):

Metabolic Syndrome ◽

Adipose Tissue ◽

Visceral Adipose Tissue ◽

Normal Structure ◽

Hepatic Tissue ◽

Standard Diet ◽

Grape Polyphenols ◽

Morphological Studies ◽

Visceral Adipose ◽

Experimental Group

Цель работы состояла в изучении влияния полифенолов винограда на органы-мишени при экспериментальном метаболическом синдроме у крыс. Методы. В течение 12 недель полифенолы винограда применялись у крыс линии Вистар. Все крысы находились на стандартном рационе. Животные были разделены на 6 групп: 1-я контрольная получала питьевую воду; 2-я контрольная и все 4 экспериментальные - 2,5% раствор фруктозы в качестве питья. 1-я экспериментальная группа дополнительно получала препарат «Фэнокор» с суммарным содержанием полифенолов 181,53 г/дм, 2-я экспериментальная - виноматериал с суммарным содержанием полифенолов 1,73 г/дм; 3-я экспериментальная - виноматериал с суммарным содержанием полифенолов 4,33 г/дм и 4-я экспериментальная - виноматериал с суммарным содержанием полифенолов 8,58 г/дм. После окончания опыта у крыс проводили морфологические исследования висцеральной жировой ткани, тканей миокарда и печени. Результаты. Анализ результатов показал, что применение полифенольных продуктов переработки винограда в концентрациях 181,53 г/дм при моделировании метаболического синдрома приводило к минимизации морфофункциональных нарушений в висцеральной жировой ткани (уменьшение интенсивности лимфоплазмоцитарной инфильтрации), миокарде (мышечные волокна имели типичное строение и адипоциты между ними встречались лишь очагово) и печени (имелись лишь слабые очаговые дистрофические изменения гепатоцитов). Заключение. Результаты работы свидетельствуют о возможности применения виноматериалов с наибольшей концентрацией полифенолов и препарата «Фэнокор» в коррекции и профилактике поражений при метаболическом синдроме. The aim of this work was to study the effect of grape polyphenols on target organs in rats with experimental metabolic syndrome. Methods. Grape polyphenols were used in Wistar rats for 12 weeks. All rats received a standard diet. The animals were divided into 6 groups: group 1, control, received drinking water; group 2, the second control, and four experimental groups received a 2.5% fructose solution for drinking. The first experimental group additionally received a drug, Fenocor, containing polyphenols at 181.53 g/dm; the second experimental group - wine material containing polyphenols at 1,73 g/dm; the third experimental group - wine material containing polyphenols at 4,33 g/dm; and the fourth experimental group - wine material containing polyphenols at 8,58 g/dm. At the end of experiment, morphological studies of visceral adipose tissue, myocardial tissue, and hepatic tissue were performed. Results. The treatment of rats with experimental metabolic syndrome with grape polyphenolic products at a concentration of 181.53 g/dm minimized morphological and functional disorders in visceral adipose tissue (intensity of lymphoplasmocytic infiltration was decreased), myocardium (muscle fibers had normal structure with only occasional adipocytes between them), and liver (only slight focal degenerative changes were observed in hepatocytes). Conclusion. The study indicated a possibility of using wine materials with the highest concentration of polyphenols and the drug Fenocor for correction and prevention of damages in metabolic syndrome.

Download Full-text