Breeding and usage of sugar beet cultivars and hybrids resistant to sugar beet nematode Heterodera schachtii

2015 ◽  
Vol 2 (1) ◽  
pp. 12-22 ◽  
Author(s):  
L. Pylypenko ◽  
K. Kalatur
Keyword(s):  
Sugar Beet ◽  
Sugar Content ◽  
Production Capacity ◽  
Nematode Resistance ◽  
Control Measures ◽  
Heterodera Schachtii ◽  
Yield Reduction ◽  
Agricultural Practice ◽  
Germplasm Exchange ◽  
Good Agricultural Practice

Heterodera schachtii Schmidt, 1871 is one of the most economically important pests of sugar beet (Beta vulgaris L.) worldwide. It is also widespread in most sugar beet growing regions in Ukraine causing serious yield reduction and decreasing sugar content of sugar beet in infested fi elds. An advanced parasitic strategy of H. schachtii is employed to support nematode growth, reproduction and harmfulness. In intensive agriculture systems the nematode control measures heavily rely on nematicides and good agricultural practice (crop rota- tion in the fi rst place). But alternative strategies based on nematode resistant sugar beet cultivars and hybrids are required as none of nematicides approved for the open fi eld application are registered in Ukraine. Here we review the achievements and problems of breeding process for H. schachtii resistance and provide the results of national traditional breeding program. Since the beginning of 1980s fi ve sugar beet cultivars (Verchnyatskyi 103, Yaltuschkivska 30, Bilotcerkivska 45, BTs-40 and Yuvileynyi) and seventeen lines partly resistant or toler- ant to H. schachtii have been obtained throughout targeted crossing and progenies assessment in the infested fi elds. The further directions for better utilization of genetic sources for nematode resistance presented in na- tional gene bank collection are emphasized. There is a need for more accurate identifi cation of resistance genes, broader application of reliable molecular markers (suitable for marker-assisted selection of nematode resistant plants in the breeding process) and methods for genetic transformation of plants. Crop cash value and national production capacity should drive the cooperation in this fi eld. Knowledge as well as germplasm exchange are thereby welcomed that can benefi t breeding progress at national and international level.

scholarly journals Influence of sowing and harvest dates on production of two different cultivars of sugar beet  

2017 ◽  
Vol 63 (No. 2) ◽  
pp. 76-81 ◽  
Author(s):  
Pavlů Klára ◽  
Chochola Jaromír ◽  
Pulkrábek Josef ◽  
Urban Jaroslav
Keyword(s):  
Sugar Beet ◽  
Sugar Content ◽  
Vegetation Period ◽  
Heterodera Schachtii ◽  
Root Yield ◽  
Harvest Dates ◽  
Small Plot ◽  
The Impact ◽  
Daily Gain ◽  
Infested Site

Small-plot trials conducted in 2013–2015 studied the impact of longer vegetation periods (by means of earlier drilling and/or later harvest) on production results of two sugar beet cultivars – one nematode-tolerant cultivar and one cultivar without such tolerance. The trials took place at two sites with different Heterodera schachtii infestation levels. In all trial seasons, root yield was significantly higher in the earlier drilled plots. On average, prolongation of the vegetation period in spring by 13 days increased root yield by 10.9%. Therefore, each day by which drilling is postponed represents a 0.7–0.8% loss of yield. As to sugar content, no statistically significant benefit of vegetation period prolongation by early drilling was found. The spring gain was slightly higher for the non-tolerant cultivar than for the tolerant one on average over all trial seasons. This result confirms the theory that nematodes impact the crop mainly in later stages of vegetation, and early drilling can thus help eliminating, to a certain degree, the risk of nematode damage. In the autumn, root yield increased by 14.3% on average over 39 days. The autumn daily gain was about half of the rate found in the spring. The increase in sugar content was between 0.6% and 1% (abs.) on average. Autumn growth achieved at the non-infested site was much higher than at the infested site.  

Sugar beet nematode Heterodera schachtii distribution and harmfulness in Ukraine

2016 ◽  
Vol 3 (3) ◽  
pp. 3-11 ◽  
Author(s):  
L. Pylypenko ◽  
K. Kalatur ◽  
J. Hallmann
Keyword(s):  
Sugar Beet ◽  
Wide Distribution ◽  
Cyst Nematode ◽  
Control Measures ◽  
Heterodera Schachtii ◽  
Prevention Measures ◽  
Economic Threshold ◽  
Beet Cyst Nematode ◽  
Effi Ciency ◽  
Sugar Beet Cyst Nematode

Aim. To determine the distribution of sugar beet cyst nematode Heterodera schachtii in Ukraine, to defi ne its population density in soil, the degree of damage and the effi ciency of control measures. Methods. Field survey for soil samples, cyst extraction by fl otation and sieving method, light microscopy. Results. The examination of 12,130 ha of agricultural fi elds in six regions of Ukraine, conducted in 2010–2015, recorded the sugar beet cyst nematode to occur in fi ve of them (Kyiv, Chernihiv, Cherkasy, Khmelnytsky, and Vinnytsia), at a total area of 2,572 ha. Taking into consideration the results of previous nematological surveys, the presence of sugar beet cyst nematode has now been documented for 18 regions of Ukraine. In most regions H. schachtii was present in 10–11 % of the examined sugar and seed producing farms with nematode population densities reaching up to 142,000 eggs and juveniles in 100 cc of soil (710-fold above the economic threshold). Conclusions. The main reasons for this wide distribution of H. schachtii in Ukraine are seen in the negligence of prevention measures, unavailability of documented data on its occurrence (missing surveys), crop rotations with over 20 % of host plants, and unavailability of effi cient nematicides and domestic nematode-resistant sugar beet cultivars.

Association of a nematode resistance bearing addition chromosome with a recurring leaf intumescence somaclonal variation in sugar beet

Genome ◽  
1991 ◽  
Vol 34 (3) ◽  
pp. 477-485
Author(s):  
M. H. Yu ◽  
L. M. Pakish ◽  
J. W. Saunders
Keyword(s):  
Sugar Beet ◽  
Somaclonal Variation ◽  
Beta Vulgaris ◽  
Chromosome Doubling ◽  
Nematode Resistance ◽  
Heterodera Schachtii ◽  
Regenerated Plants ◽  
Alternative Expression ◽  
Monosomic Addition

Intumescent leaf variants of sugar beet (Beta vulgaris L.) were obtained through callus culture of a monosomic addition that carried resistance to Heterodera schachtii Schm. The frothy pockmarked appearance of the leaf surface was due to hyperplastic growth of the mesophyll and epidermal cells. The epidermis had many malformed stomata. Veins were underdeveloped, but protrusions beneath were pronounced. Intumescence occurred in 20.3% of the regenerated plants and it was heritable to F1 and later progeny. Leaf intumescence is a new phenotype for Beta. About 73.5% of regenerants contained the donor somatic chromosome number, the remainder were doubled or mixoploids, with no chromosome losses apparent. The 38-chromosome intumescent plant represents a dual somaclonal variation, chromosome doubling and leaf intumescence. Progeny of the 19- and 38-chromosome intumescent plants intercrossed or pollinated by diploids or tetraploids had 9, 18, 19, 27, 28, 29, 36, 37, 38, or 39 chromosomes. All intumescent plants were aneuploids with the monosome addition. There were linkages for leaf intumescence (Li), resistance to H. schachtii (Hs), and hypocotyl color (Rpro) on the addition chromosome. The efficacy of Hs remained intact through the in vitro culture and succeeding crosses. The Li-bearing plants manifested depressed growth and markedly reduced seed set. Leaf intumescence was thought to be the alternative expression of galling potential of Beta procumbens Chr. Sm. germ plasm.Key words: somaclonal variation, leaf intumescence, nematode resistance, monosomic addition, Beta vulgaris L.

scholarly journals The influence of non-chemical weed control methods on sugar beet productivity and quality parameters

2019 ◽  
Vol 26 (1) ◽  
Author(s):  
Jovita Balandaitė ◽  
Aida Adamavičienė ◽  
Kęstutis Romaneckas ◽  
Edita Eimutytė
Keyword(s):  
Sugar Beet ◽  
Weed Control ◽  
Spring Barley ◽  
Sugar Content ◽  
Quality Parameters ◽  
Control Measures ◽  
Control Treatment ◽  
Control Methods ◽  
White Mustard ◽  
Cutting Out

A long-term stationary field experiment was carried out at the Experimental Station of Aleksandras Stulginskis University (Vytautas Magnus University Agriculture Academy since 2019) in 2017. The following sustainable weed control methods were examined: 1) inter-row loosening (control treatment); 2) inter-row cutting and mulching with weeds; 3) inter-row cutting and mulching with Persian clover; 4) inter-row cutting and mulching with white mustards; 5) interrow cutting and mulching with spring barley. The alternatives to weed control often reduced the yields of sugar beet roots significantly, however, when mulching with white mustard the decrease in fertility was not essential. Non-chemical weed control measures in most cases had an insignificant effect on the parameters of sugar beet quality – the content of Na, K and alpha-amino N. The most significant sugar content (16.34 and 16.26%) was found in sugar beets, which were grown applying interrow mulch of spring barley and weed (P < 0.05). Although insignificant, but the largest index of sugar beet leaf area was found in the experimental plots, where inter-row cutting out and mulching with white mustard were applied. Comparing non-chemical weed control systems, the highest amount of white (crystalline) sugar (4.96 ha–1) was derived from the sugar beet that was grown using white mustard mulch.

scholarly journals BvcZR3 and BvHs1pro-1 Genes Pyramiding Enhanced Beet Cyst Nematode (Heterodera schachtii Schm.) Resistance in Oilseed Rape (Brassica napus L.)

2019 ◽  
Vol 20 (7) ◽  
pp. 1740 ◽  
Author(s):  
Xuanbo Zhong ◽  
Qizheng Zhou ◽  
Nan Cui ◽  
Daguang Cai ◽  
Guixiang Tang
Keyword(s):  
Transgenic Plants ◽  
Sugar Beet ◽  
Oilseed Rape ◽  
Gene Pyramiding ◽  
Nematode Resistance ◽  
Cyst Nematode ◽  
Heterodera Schachtii ◽  
Translocation Line ◽  
Wild Type ◽  
Beet Cyst Nematode

Beet cyst nematode (Heterodera schachtii Schm.) is one of the most damaging pests in sugar beet growing areas around the world. The Hs1pro-1 and cZR3 genes confer resistance to the beet cyst nematode, and both were cloned from sugar beet translocation line (A906001). The translocation line carried the locus from B. procumbens chromosome 1 including Hs1pro-1 gene and resistance gene analogs (RGA), which confer resistance to Heterodera schachtii. In this research, BvHs1pro-1 and BvcZR3 genes were transferred into oilseed rape to obtain different transgenic lines by A. tumefaciens mediated transformation method. The cZR3Hs1pro-1 gene was pyramided into the same plants by crossing homozygous cZR3 and Hs1pro-1 plants to identify the function and interaction of cZR3 and Hs1pro-1 genes. In vitro and in vivo cyst nematode resistance tests showed that cZR3 and Hs1pro-1 plants could be infested by beet cyst nematode (BCN) juveniles, however a large fraction of penetrated nematode juveniles was not able to develop normally and stagnated in roots of transgenic plants, consequently resulting in a significant reduction in the number of developed nematode females. A higher efficiency in inhibition of nematode females was observed in plants expressing pyramiding genes than in those only expressing a single gene. Molecular analysis demonstrated that BvHs1pro-1 and BvcZR3 gene expressions in oilseed rape constitutively activated transcription of plant-defense related genes such as NPR1 (non-expresser of PR1), SGT1b (enhanced disease resistance 1) and RAR1 (suppressor of the G2 allele of skp1). Transcript of NPR1 gene in transgenic cZR3 and Hs1pro-1 plants were slightly up-regulated, while its expression was considerably enhanced in cZR3Hs1pro-1 gene pyramiding plants. The expression of EDS1 gene did not change significantly among transgenic cZR3, Hs1pro-1 and cZR3Hs1pro-1 gene pyramiding plants and wild type. The expression of SGT1b gene was slightly up-regulated in transgenic cZR3 and Hs1pro-1 plants compared with the wild type, however, its expression was not changed in cZR3Hs1pro-1 gene pyramiding plant and had no interaction effect. RAR1 gene expression was significantly up-regulated in transgenic cZR3 and cZR3Hs1pro-1 genes pyramiding plants, but almost no expression was found in Hs1pro-1 transgenic plants. These results show that nematode resistance genes from sugar beet were functional in oilseed rape and conferred BCN resistance by activation of a CC-NBS-LRR R gene mediated resistance response. The gene pyramiding had enhanced resistance, thus offering a novel approach for the BCN control by preventing the propagation of BCN in oilseed rape. The transgenic oilseed rape could be used as a trap crop to offer an alternative method for beet cyst nematode control.

scholarly journals Impact of Anthracnose on Avocado Production in Kenya

HortScience ◽  
2005 ◽  
Vol 40 (4) ◽  
pp. 1103D-1104
Author(s):  
Lusike A. Wasilwa ◽  
Joseph K. Njuguna ◽  
Violet Kirigua ◽  
Charles N. Waturu ◽  
Richelle A. Stafne ◽  
...  
Keyword(s):  
Large Scale ◽  
Control Measures ◽  
Fruit Rot ◽  
Agricultural Practice ◽  
Pests And Diseases ◽  
Planting Material ◽  
Good Agricultural Practice ◽  
Postharvest Handling ◽  
The Impact ◽  
European Markets

Avocado is the leading horticultural export in Kenya. In 2003, Kenya exported about 19,000 t, compared to 23 t in 1970. Most of the fruit is exported to the European markets. There are several constraints limiting production of avocado in Kenya, including limited superior varieties or planting material, poor infrastructure, poor market information, and poor tree crop management. Although several diseases infect avocado, the most important are fruit rot pathogens, such as anthracnose, cercospora, and scab. However, diseases and pests have not been important to avocado production in Kenya. Recently (2004), the constraint limiting avocado production in Kenya is anthracnose, caused by Colletotrichum gloeospoirioides. Little is known in Kenya on the impact of this disease on production and income realized by small-(<1 acre) or large-scale growers. The objectives of this research were to quantify losses attributed to anthracnose in Kenya, determine the current disease control measures, and recommend good agricultural practice in conformance with EUREP-GAP. A survey was conducted in the avocado growing areas to identify and document constraints limiting avocado production in 2004. The highest losses were reported from central Kenya, where exporters reported up to 100% losses. Because all avocado varieties growing in Kenya are susceptible to anthracnose, it is recommended that good management, i.e., control of pests and diseases and good postharvest handling of fruit to minimize injury used as an entry point by anthracnose, should be enforced.

Long-term storability of different sugarbeet genotypes – Results of a joint IIRB study

2010 ◽  
pp. 661-667 ◽  
Author(s):  
Noud van Swaaij ◽  
Toon Huijbregts
Keyword(s):  
Storage Temperature ◽  
Abiotic Factors ◽  
Sugar Content ◽  
Amino Nitrogen ◽  
Root Tip ◽  
Soluble Nitrogen ◽  
Agricultural Practice ◽  
Good Agricultural Practice ◽  
Before And After

In 2008/09 and 2009/10, storage trials with 12 sugarbeet genotypes were carried out under different conditions in six countries. The genotypes were grown in strips and harvested in September/November either by machine (using good agricultural practice) or by hand. Samples were then stored either in nets incorporated in clamps or in separate nets/bags or containers inside a barn or climate room. All samples were weighed and analyzed before and after storage for sugar (by polarimetry), potassium, sodium, amino nitrogen, total soluble nitrogen, sucrose, glucose, fructose, raffinose, betaine and glutamine content. After storage the samples were also examined visually. Differences between the genotypes were observed for root tip breakage, sprouting, moulds and rot, although these differences were not unambiguous in all experiments and varied between the observations in the different countries. The sugar losses ranged from 0 to 66% of the initial amount and seemed to be related to various biotic and abiotic factors. Root damage by machine harvest and storage temperature were dominant factors in relation to the sugar losses. Genotypes also showed significant differences in sugar losses, but a strong interaction with year and site existed. Correlations could be found between sugar losses and initial sugar content (r = –0.66), initial betaine content (r = –0.62) and root tip breakage (r = +0.66) and after storage, moulds (r = +0.87), rot (r = +0.88) and invert sugars (r = +0.89). Chemical analyses showed differences between the genotypes for the decrease in beet quality after storage, not only by a reduction in sugar content but also by an increase in invert sugar and soluble nitrogen.

Influence of a BactoFil microbiological preparation on the intensity of infection by Rhizoctonia solani and the effects on sugar beet yield and quality

2012 ◽  
pp. 102-109
Author(s):  
Suzana Kristek ◽  
Andrija Kristek ◽  
Dragana Kocevski ◽  
Antonija K. Jankovi ◽  
Dražen Juriši
Keyword(s):  
Sugar Beet ◽  
Rhizoctonia Solani ◽  
Nitrogen Fertilizer ◽  
Soil Analysis ◽  
Block Design ◽  
Sugar Content ◽  
Pathogenic Fungi ◽  
Fertilizer Application ◽  
Beet Root ◽  
Set Up

The experiment was set up on two types of the soil: Mollic Gleysols (FAO, 1998) and Eutric Cambisols where the presence of pathogenic fungi – sugar beet root decay agent – Rhizoctonia solani has been detected since 2005. In a two year study (2008, 2009), the experiment was set up by completely randomized block design in 4 repetitions and 16 different variants. Two beet varieties, Belinda, sensitive to pathogenic fungi R. solani, and Laetitia, tolerant to pathogenic fungi R. solani), were grown. The microbiological preparation BactoFil was applied in different amounts in autumn and spring. In addition, the nitrogen fertilizer application, based on the results of soil analysis, was varied. The following parameters were tested: amount of infected and decayed plants, root yield, sugar content, sugar in molasses and sugar yield. The best results were obtained by applying the microbiological preparation BactoFil, and by 30% reduced nitrogen fertilizer application. Preparation dosage and time of application depended on soil properties.

Susceptibility to root tip breakage increases storage losses of sugar beet genotypes

2016 ◽  
pp. 625-632 ◽  
Author(s):  
Christa Hoffmann ◽  
Katharina Schnepel
Keyword(s):  
Sugar Beet ◽  
Sugar Content ◽  
Close Correlation ◽  
Invert Sugar ◽  
Root Tip ◽  
Sugar Accumulation ◽  
Genotypic Differences ◽  
Storage Losses ◽  
Controlled Conditions ◽  
Sugar Loss

Good storability of sugar beet is of increasing importance, not only to reduce sugar losses, but also with regard to maintaining the processing quality. Genotypic differences are found in storage losses. However, it is not clear to which extent damage may contribute to the genotypic response. The aim of the study was to quantify the effect of root tip breakage on storage losses of different genotypes. For that purpose, in 2012 and 2013, six sugar beet genotypes were grown in field trials at two locations. After lifting roots were damaged with a cleaning device. They were stored for 8 and 12 weeks, either under controlled conditions in a climate container at constant 8°C, or under ambient temperature in an outdoor clamp. The close correlation underlines that storage losses under controlled conditions (constant temperature) can well be transferred to conditions in practice with fluctuating temperature. The strongest impact on invert sugar accumulation and sugar loss after storage resulted from storage time, followed by damage and growing environment (year × growing site). Cleaning reduced soil tare but increased root tip breakage, in particular for genotypes with low marc content. During storage, pathogen infestation and invert sugar content of the genotypes increased with root tip breakage, but the level differed between growing environments. Sugar loss was closely related to invert sugar accumulation for all treatments, genotypes and environments. Hence, it can be concluded that root tip breakage contributes considerably to storage losses of sugar beet genotypes, and evidently genotypes show a different susceptibility to root tip breakage which is related to their marc content. For long-term storage it is therefore of particular importance to avoid damage during the harvest operations and furthermore, to have genotypes with high storability and low susceptibility to damage.

Development of the sugar beet quality in the Netherlands since 1980 and introduction of invert sugar as a new parameter for beet quality assessment

2015 ◽  
pp. 95-103 ◽  
Author(s):  
Dirk P. Vermeulen
Keyword(s):  
The Netherlands ◽  
Sugar Beet ◽  
Quality Assessment ◽  
Sugar Content ◽  
Quality Parameters ◽  
Invert Sugar ◽  
Internal Quality ◽  
Glucose Content ◽  
Thick Juice ◽  
Trial Phase

The technological beet quality has been always important for the processors of sugar beet. An investigation into the development of the beet quality in the Netherlands since 1980 has shown that beet quality has improved significantly. Internal quality parameters that are traditionally determined in the beet laboratory, i.e. sugar content, Na, K and -aminoN, all show an improving trend over the years. In the factories, better beet quality has led to lower lime consumption in the juice purification and significantly higher thick juice purity. In 2013, Suiker Unie introduced the serial analysis of the glucose content in beet brei as part of the routine quality assessment of the beet. The invert sugar content is subsequently calculated from glucose content with a new correlation. The background, the trial phase and the first experiences with the glucose analyzer are discussed.

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