scholarly journals Androgenic studies in the production of haploids and doubled haploids in Capsicum spp.

2020 ◽  
Vol 73 (1) ◽  
pp. 9047-9056 ◽  
Author(s):  
Manuel Alejandro Sánchez ◽  
Yacenia Morillo Coronado ◽  
Ana Cruz Morillo Coronado
Keyword(s):  
Genetic Variability ◽  
Double Haploid ◽  
Genetic Improvement ◽  
Doubled Haploids ◽  
Horticultural Crop ◽  
Breeding Programs ◽  
Agronomic Characteristics ◽  
The World ◽  
Capsicum Spp ◽  
The Tropics

Capsicum spp. is a horticultural crop of agronomic interest and is considered the fourth most important vegetable in the world. It is an important nutritional and medicinal source, and its production generates employment in the tropics. In this species, the genetic variability is wide and with great potential, which has been exploited to generate outstanding varieties. Breeding programs seek different alternatives to accelerate the production of improved varieties with desirable agronomic characteristics. These objectives can be achieved with the production of haploid and double haploid plants via androgenesis or gynogenesis, being androgenesis the approach most used for paprika cultures. The purpose of this review is to present the results of different researches in obtaining haploids and doubled haploids in cultivars of Capsicum spp. and its impact on the genetic improvement of this crop. 

scholarly journals Double Haploid Development and Assessment of Androgenic Competence of Balkan Pepper Core Collection in Bulgaria

Plants ◽  
2021 ◽  
Vol 10 (11) ◽  
pp. 2414
Author(s):  
Stanislava Grozeva ◽  
Gancho Pasev ◽  
Vesela Radeva-Ivanova ◽  
Velichka Todorova ◽  
Valentina Ivanova ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Double Haploid ◽  
Genetic Improvement ◽  
Core Collection ◽  
Culture Medium ◽  
Doubled Haploids ◽  
Caps Marker ◽  
Regenerated Plants ◽  
Direct Embryogenesis ◽  
Pepper Breeding

This study was designed to assess the androgenic potential of 180 pepper accessions and 11 progenies (four F1 and seven BC) possessing PMMoV resistance in order to complement an ongoing pepper breeding program. The experiment was carried out in 10 replications with 20 anthers for each accession in two different induction mediums from 2017 to 2019. The highest androgenic response was observed in culture medium 17-2 but differences between two mediums were nonsignificant. From a total of 191 genotypes, 102 genotypes expressed a potential for direct embryogenesis. Embryo induction was seen to be genotype-dependent and decreased in the following order: Pumpkin > Conical > Bell or blocky > Round > Elongate as the most responsive genotypes with over 10% reacted anthers being observed in CAPS-23, CAPS-29, CAPS-127, CAPS-157, CAPS-169, F1 and BC 887 derived from CAPS-23. The number of regenerated plants was higher in the conical group and least in the round varietal group. Regenerated plants were examined visually and by flow cytometry for identification of spontaneous doubled haploids (DH) and haploids. Those originating from F1 and BC progenies were additionally evaluated by a CAPS marker targeting L4 allele for resistance against PMMoV. Obtained results revealed two groups consisting of homozygous susceptible and resistant plants. Therefore, use of anther culture in ongoing breeding will greatly facilitate the pepper genetic improvement.

scholarly journals VARIABILIDADE GENÉTICA UTILIZANDO DESCRITORES MORFOLÓGICOS PARA ACESSOS DE Capsicum spp. PARA FINS ORNAMENTAIS

Nativa ◽  
2019 ◽  
Vol 7 (6) ◽  
pp. 675
Author(s):  
Petterson Baptista da Luz ◽  
Alessandro Aparecido Brito dos Santos ◽  
Valdete Campos Ambrosio
Keyword(s):  
Genetic Variability ◽  
Ornamental Plants ◽  
Growth Potential ◽  
Breeding Programs ◽  
Canonical Correlations ◽  
Plant Parts ◽  
Potted Plants ◽  
Capsicum Spp ◽  
Biometric Templates

O mercado de pimentas para fins ornamentais é um setor recém explorado, mas com grande potencial de crescimento no ramo de floricultura e paisagismo. As pimenteiras que possuem folhagem variegada, porte baixo, frutos com coloração variada em seus diferentes estádios de maturação, contrastando com as folhagens, já são utilizadas como plantas ornamentais, cultivadas e comercializadas em vasos. O presente trabalho teve como objetivo avaliar a variabilidade genética visando o potencial ornamental de acessos de Capsicum spp. através de modelos biométricos. Foram utilizados 55 acessos de Capsicum spp, e a caracterização morfoagronômica dos acessos foi realizada avaliando diferentes partes da planta. O terceiro par de correlações canônicas, com correlação de 0,51, associa plantas com área do dossel compacto-densa, com menor comprimento e largura de dossel e de pequeno porte, associado com folhagens estreitas e de pecíolo grande. Esta associação é vantajosa para ornamentação. Ficou evidenciada a presença de variabilidade genética na população, o que é bastante favorável ao melhoramento na medida em que a heterogeneidade genética possibilita a obtenção de ganhos por meio de seleção.Palavras-chave: pimenta; modelos biométricos; correlações canônicas; análise de trilha. GENETIC VARIABILITY FOCUSED ON ORNAMENTAL POTENTIAL OF A Capsicum spp. ACCESSION ABSTRACT: Pepper market for ornamental purposes is recently exploited; however, it has a great growth potential in floriculture and landscaping. Pepper with variegated foliage, small size, and varied fruit coloration at different ripening stages contrasting with leaves have already been used as ornamental plants cultivated and purchased as potted plants. This study aimed to evaluate genetic variability of Capsicum spp. focusing on its ornamental potential by means of biometric templates. Fifty-five Capsicum spp. accessions were used and a morpho-agronomic characterization of the accessions were performed by evaluating different plant parts. The third canonical correlations are 0.51 and it associates compact-dense, small, shorter length and width canopy plants with narrow foliage and large petiole, which is advantageous for ornamentation. Genetic variation was evidenced in the population being very favorable for plant breeding programs once genetic heterogeneity enables obtaining gains through selections.Keywords: pepper; biometric templates; canonic correlations; trail analysis.

scholarly journals STUDYING SELECTION CRITERIA AND GENETIC VARIABILITY FOR IMPROVEMENT OF INDIGENOUS MAIZE IN PAKISTAN

2019 ◽  
Vol 56 (04) ◽  
pp. 819-827
Author(s):  
Muhammad Amir Maqbool
Keyword(s):  
Genetic Variability ◽  
Genetic Improvement ◽  
Selection Criteria ◽  
Agronomic Traits ◽  
Yield Potential ◽  
Path Coefficient ◽  
Breeding Programs ◽  
Maize Germplasm ◽  
Maize Genotypes ◽  
Yield Penalty

Maize (Zea mays L.) is short duration, high yielding crop, and it can be grown in both spring and kharif seasons in Pakistan. In current study white and yellow maize germplasms were compared for correlations and genetic variability based on different agronomic traits to define selection criteria for maize improvement. Significant differences were observed for yield and related components for entries, checks, all tested genotypes, yellow and white germplasm. Yellow maize genotypes showed higher yield potential compared to white maize genotypes because of more grains per row and number of rows per cob. Based on correlation and path coefficient analysis, yield per plant was proved as appropriate selection criteria for white maize whereas, yield per plant and grains per row were suitable selection criteria for genetic improvement of yellow maize. Total carotenoid contents of yellow maize were independent of the yield so, both can be simultaneously targeted for genetic improvement without paying yield penalty. However, further dissection of genetic variability in yellow maize germplasm based on provitamin A carotenoids is prerequisite. So, far as genotypes, 19189, 15159, 19201, 15018, 15216, 15170, 15155, 19196, 15318 and 19174 among white maize germplasm whereas, 14965, 14982, 19205, 15019, 14971, 15163, 15205, 24687, 15207 and 15194 among yellow maize germplasm were the potential high yielding genotypes. Findings of the study in terms of selection criteria and potential maize genotypes could be useful in different breeding programs for genetic improvement of maize

scholarly journals Genetic variability in Tambaqui generations of the breeding program in the Central-West Region of Brazil

2021 ◽  
Vol 42 (3Supl1) ◽  
pp. 1785-1796
Author(s):  
Angela Maria Urrea-Rojas ◽  
◽  
Annaiza Braga Bignardi ◽  
Felipe Pinheiro de Souza ◽  
Ed Christian Suzuki de Lima ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Genetic Variability ◽  
Native Species ◽  
Genetic Improvement ◽  
Improvement Program ◽  
Breeding Programs ◽  
Mato Grosso ◽  
West Region ◽  
Fish Breeding ◽  
The Mean

The implementation of fish breeding programs in Brazil has brought significant results in the productivity of tilapia. However, the insertion of native species with great potential (such as Tambaqui Colossoma macropomum) in these programs is still recent, and thus requires genetic information for monitoring and enabling their consolidation into the programs. The objective of the present study was to evaluate the genetic diversity of the parental generation (G0) and two consecutive generations (G1 and G2) in the C. macropomum genetic improvement program, located in the municipality of Sorriso, Mato Grosso, Brazil. Ninety caudal fin samples were collected (30 samples per generation) for DNA extraction. The genetic study implemented seven microsatellite markers (Cm1A8, Cm1A11, Cm1D1, Cm1E3, Cm1F4, Cm1F5, and Cm1H8). A total of 17 alleles were amplified, with variations in the mean number between four to two alleles per locus. The size per locus ranged from 170 to 360 bp. The average inbreeding coefficient was 0.126 (G0), -0.040 (G1), and 0.131 (G2). No null or exclusive alleles were found. The observed heterozygosity values for G1 and G2 demonstrated the preservation of genetic variability (0.453 and 0.409, respectively). In conclusion, the genetic diversity of the parental generation (G0) and the two progenies generations (G1 and G2) were adequate, which demonstrates that the genetic improvement program was conducted correctly; however, it is important to continue to evaluations the genetic diversity of the future progeny.

scholarly journals Plant breeding and in situ utilization of palm trees

2012 ◽  
Vol 42 (2) ◽  
pp. 261-269 ◽  
Author(s):  
Mercedes Rivas ◽  
Rosa Lía Barbieri ◽  
Luciano Carlos da Maia
Keyword(s):  
Plant Breeding ◽  
Elaeis Guineensis ◽  
Local Development ◽  
Phoenix Dactylifera ◽  
Palm Tree ◽  
Breeding Programs ◽  
Palm Trees ◽  
The World ◽  
The Tropics

The palm tree family (Arecaceae) is constituted by approximately 3000 species mainly distributed in the tropics and subtropics. As a source of a variety of products they contribute to the world and local economies, and also to peoples lifestyles. Historically their use has been based on wild populations, but also on local domestication. Very few species are subject of plant breeding programs and are cultivated in the world. This is the case of the African oil palm (Elaeis guineensis), in which investment and development consortiums invest high sums. Another kind of crop is the date palm (Phoenix dactylifera), which was domesticated thousand of years ago and whose success is based in the export of a fine product with worldwide recognition. In this case the production is based on traditional varieties and has very incipient breeding programs. A third group of palms includes those species from which products are obtained and manufactured for local development. The objective of this literature review is to contribute in the analysis of opportunities and weaknesses to investing in domestication and plant breeding programs in those palm trees with a recognized productive value.

scholarly journals Genetic variability and genome-wide association analysis of flavor and texture in cooked beans (Phaseolus vulgaris L.)

2021 ◽  
Author(s):  
Amber Bassett ◽  
Kelvin Kamfwa ◽  
Daniel Ambachew ◽  
Karen Cichy
Keyword(s):  
Genetic Variability ◽  
Seed Coat ◽  
Principal Component ◽  
Breeding Programs ◽  
Flavor Intensity ◽  
Genome Wide ◽  
White Bean ◽  
The Usa ◽  
New Varieties ◽  
End Use

Abstract Key message Cooked bean flavor and texture vary within and across 20 Andean seed types; SNPs are significantly associated with total flavor, beany, earthy, starchy, bitter, seed-coat perception, and cotyledon texture. Abstract Common dry beans are a nutritious food recognized as a staple globally, but their consumption is low in the USA. Improving bean flavor and texture through breeding has the potential to improve consumer acceptance and suitability for new end-use products. Little is known about genetic variability and inheritance of bean sensory characteristics. A total of 430 genotypes of the Andean Diversity Panel representing twenty seed types were grown in three locations, and cooked seeds were evaluated by a trained sensory panel for flavor and texture attribute intensities, including total flavor, beany, vegetative, earthy, starchy, sweet, bitter, seed-coat perception, and cotyledon texture. Extensive variation in sensory attributes was found across and within seed types. A set of genotypes was identified that exhibit extreme attribute intensities generally stable across all three environments. seed-coat perception and total flavor intensity had the highest broad-sense heritability (0.39 and 0.38, respectively), while earthy and vegetative intensities exhibited the lowest (0.14 and 0.15, respectively). Starchy and sweet flavors were positively correlated and highest in white bean genotypes according to principal component analysis. SNPs associated with total flavor intensity (six SNPs across three chromosomes), beany (five SNPs across four chromosomes), earthy (three SNPs across two chromosomes), starchy (one SNP), bitter (one SNP), seed-coat perception (three SNPs across two chromosomes), and cotyledon texture (two SNPs across two chromosomes) were detected. These findings lay a foundation for incorporating flavor and texture in breeding programs for the development of new varieties that entice growers, consumers, and product developers alike.

scholarly journals Recent advances in CRISPR/Cas9 and applications for wheat functional genomics and breeding

aBIOTECH ◽  
2021 ◽  
Author(s):  
Jun Li ◽  
Yan Li ◽  
Ligeng Ma
Keyword(s):  
Functional Genomics ◽  
Genome Editing ◽  
Functional Annotation ◽  
Genome Engineering ◽  
Agronomic Traits ◽  
Wheat Breeding ◽  
Breeding Programs ◽  
Base Editing ◽  
The World ◽  
World Food Security

AbstractCommon wheat (Triticum aestivum L.) is one of the three major food crops in the world; thus, wheat breeding programs are important for world food security. Characterizing the genes that control important agronomic traits and finding new ways to alter them are necessary to improve wheat breeding. Functional genomics and breeding in polyploid wheat has been greatly accelerated by the advent of several powerful tools, especially CRISPR/Cas9 genome editing technology, which allows multiplex genome engineering. Here, we describe the development of CRISPR/Cas9, which has revolutionized the field of genome editing. In addition, we emphasize technological breakthroughs (e.g., base editing and prime editing) based on CRISPR/Cas9. We also summarize recent applications and advances in the functional annotation and breeding of wheat, and we introduce the production of CRISPR-edited DNA-free wheat. Combined with other achievements, CRISPR and CRISPR-based genome editing will speed progress in wheat biology and promote sustainable agriculture.

scholarly journals Unveiling a unique genetic diversity of cultivated Coffea arabica L. in its main domestication center: Yemen

2021 ◽  
Author(s):  
C. Montagnon ◽  
A. Mahyoub ◽  
W. Solano ◽  
F. Sheibani
Keyword(s):  
Genetic Diversity ◽  
Genetic Distance ◽  
Coffea Arabica ◽  
Genetic Improvement ◽  
The World ◽  
Genetic Clusters ◽  
Domestication Process ◽  
Almost All ◽  
Coffea Arabica L ◽  
Cup Quality

AbstractWhilst it is established that almost all cultivated coffee (Coffea arabica L.) varieties originated in Yemen after some coffee seeds were introduced into Yemen from neighboring Ethiopia, the actual coffee genetic diversity in Yemen and its significance to the coffee world had never been explored. We observed five genetic clusters. The first cluster, which we named the Ethiopian-Only (EO) cluster, was made up exclusively of the Ethiopian accessions. This cluster was clearly separated from the Yemen and cultivated varieties clusters, hence confirming the genetic distance between wild Ethiopian accessions and coffee cultivated varieties around the world. The second cluster, which we named the SL-17 cluster, was a small cluster of cultivated worldwide varieties and included no Yemen samples. Two other clusters were made up of worldwide varieties and Yemen samples. We named these the Yemen Typica-Bourbon cluster and the Yemen SL-34 cluster. Finally, we observed one cluster that was unique to Yemen and was not related to any known cultivated varieties and not even to any known Ethiopian accession: we name this cluster the New-Yemen cluster. We discuss the consequences of these findings and their potential to pave the way for further comprehensive genetic improvement projects for the identification of major resilience/adaptation and cup quality genes that have been shaped through the domestication process of C. arabica.

scholarly journals Genetic analysis of sucrose concentration in soybean seeds using a historical soybean genomic panel

2021 ◽  
Author(s):  
Alexandra Ficht ◽  
Robert W. Bruce ◽  
Davoud Torkamaneh ◽  
Christopher Grainger ◽  
Milad Eskandari ◽  
...  
Keyword(s):  
Genetic Variability ◽  
Genome Wide Association Study ◽  
Sucrose Concentration ◽  
Soybean Seed ◽  
Breeding Programs ◽  
Snp Data ◽  
Genome Wide ◽  
A Genome ◽  
Important Trait ◽  
Genotype By Sequencing

Abstract Soybean (Glycine max (L.) Merr) is a crop of global importance for both human and animal consumption, which was domesticated in China more than 6000 years ago. A concern about losing genetic diversity as a result of decades of breeding has been expressed by soybean researchers. In order to develop new cultivars, it is critical for breeders to understand the genetic variability present for traits of interest in their program germplasm. Sucrose concentration is becoming an increasingly important trait for the production of soy-food products. The objective of this study was to use a genome-wide association study (GWAS) to identify putative QTL for sucrose concentration in soybean seed. A GWAS panel consisting of 266 historic and current soybean accessions was genotyped with 76k genotype-by-sequencing (GBS) SNP data and phenotyped in four field locations in Ontario (Canada) from 2015 to 2017. Seven putative QTL were identified on chromosomes 1, 6, 8, 9, 10, 13 and 14. A key gene related to sucrose synthase (Glyma.06g182700) was found to be associated with the QTL found on chromosome 6. This information will facilitate efforts to increase the available genetic variability for sucrose concentration in soybean breeding programs and develop new and improved high-sucrose soybean cultivars suitable for the soy-food industry.

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