Cadmium(II) and Lead(II) removal by Chlorella sp. Immobilized and E. coli genetically engineered with mice Metallothionein I

2010 ◽  
Vol 1278 ◽  
Author(s):  
V. Almaguer-Cantú ◽  
L. Morales-Ramos ◽  
K. Arevalo-Niño ◽  
M.T. Garza-González ◽  
I. Balderas-Rentería
Keyword(s):  
Heavy Metals ◽  
Aqueous Solutions ◽  
Metal Concentration ◽  
New Technologies ◽  
Genetically Engineered ◽  
Suitable Material ◽  
E Coli ◽  
Chlorella Sp ◽  
Heavy Metals Ions ◽  
Langmuir Constants

AbstractThe pollution caused by heavy metals is one of the major environmental problems that is imperative to be solved. New technologies, easy to implement and to adapt to any system, deserve special attention and are a focus of this work the ability of Chlorella sp. and E. coli genetically engineered with mice metallothionein I, both immobilized in alginate of calcium to remove Cd(II) and Pb(II) from aqueous solutions was investigated in batch assays for the treatment of diluted aqueous solutions. The kinetics, sorption capacities and sorption percentage were determined. The influence of metal concentration in solution is discussed in the terms of Langmuir isotherm and constants. Sorption capacities increased with increasing metal concentration in solution. For solution containing 300 mg/L of metal, the observed uptake capacities were 94.941±1.094 mgCd/gChlorella., 24.076±2.292 mgCd/gE.coli and 239.17±2.478 mgPb/gChlorella, 37.952±4.245 mgPb/gE.coli. The Langmuir constants to Chlorella sp. were qmax=285.72(mgPb/g), b=0.0276(l/mgPb), qmax=103.65(mgCd/g) and b=0.0005(l/mgCd) while to E. coli were qmax=28.141(mgPb/g), b=0.113(l/mgPb), qmax=24.272(mgCd/g) and b =0.019(1/mgCd). The biomass of the algae showed to have better capacity of metallic sorption that the biomass of the bacteria genetically engineering. The study proved that microorganisms biomass is a suitable material for the removal of the studied heavy metals ions from aqueous solutions, achieving removal efficiencies higher than 90%, and could be considered as a potential material for treating effluent polluted with Cd(II) and Pb(II) ions.

Cadmium(II) and Lead(II) removal by Chlorella sp. Immobilized and E. coli genetically engineered with mice Metallothionein I

2010 ◽  
Vol 1277 ◽  
Author(s):  
V. Almaguer-Cantú ◽  
L. Morales-Ramos ◽  
K. Arevalo-Niño ◽  
M.T. Garza-González ◽  
I. Balderas-Rentería
Keyword(s):  
Heavy Metals ◽  
Aqueous Solutions ◽  
Metal Concentration ◽  
New Technologies ◽  
Genetically Engineered ◽  
Suitable Material ◽  
E Coli ◽  
Chlorella Sp ◽  
Heavy Metals Ions ◽  
Langmuir Constants

The pollution caused by heavy metals is one of the major environmental problems that is imperative to be solved. New technologies, easy to implement and to adapt to any system, deserve special attention and are a focus of this work the ability of Chlorella sp. and E. coli genetically engineered with mice metallothionein I, both immobilized in alginate of calcium to remove Cd(II) and Pb(II) from aqueous solutions was investigated in batch assays for the treatment of diluted aqueous solutions. The kinetics, sorption capacities and sorption percentage were determined. The influence of metal concentration in solution is discussed in the terms of Langmuir isotherm and constants. Sorption capacities increased with increasing metal concentration in solution. For solution containing 300 mg/L of metal, the observed uptake capacities were 94.941±1.094 mgCd/gChlorella., 24.076±2.292 mgCd/gE.coli and 239.17±2.478 mgPb/gChlorella, 37.952±4.245 mgPb/gE.coli. The Langmuir constants to Chlorella sp. were qmax=285.72(mgPb/g), b=0.0276(l/mgPb), qmax=103.65(mgCd/g) and b=0.0005(l/mgCd) while to E. coli were qmax=28.141(mgPb/g), b=0.113(l/mgPb), qmax=24.272(mgCd/g) and b =0.019(l/mgCd). The biomass of the algae showed to have better capacity of metallic sorption that the biomass of the bacteria genetically engineering. The study proved that microorganisms biomass is a suitable material for the removal of the studied heavy metals ions from aqueous solutions, achieving removal efficiencies higher than 90%, and could be considered as a potential material for treating effluent polluted with Cd(II) and Pb(II) ions.

Preconcentration of heavy metals ions from aqueous solutions by means of cellulose phosphate: an application in water analysis

Talanta ◽  
1997 ◽  
Vol 45 (2) ◽  
pp. 317-323 ◽  
Author(s):  
P DEMAGALHAESPADILHA ◽  
J ROCHA ◽  
J MOREIRA ◽  
J DESOUSACAMPOS ◽  
C DOCARMOFEDERICI
Keyword(s):  
Heavy Metals ◽  
Aqueous Solutions ◽  
Water Analysis ◽  
Cellulose Phosphate ◽  
Heavy Metals Ions

Biosorption of chromium(VI), nickel(II) and Remazol Blue by Rhodotorula muciloginosa biomass

2012 ◽  
Vol 65 (3) ◽  
pp. 471-477 ◽  
Author(s):  
Nalan Oya San ◽  
Gönül Dönmez
Keyword(s):  
Heavy Metals ◽  
Heavy Metal ◽  
Aqueous Solutions ◽  
Metal Concentration ◽  
Metal Ion ◽  
Heavy Metal Concentration ◽  
Reactive Dye ◽  
Ion Concentration ◽  
Chromium Vi

The passive removal of commonly used reactive dye and two heavy metals, from aqueous solutions by inexpensive biomaterial, yeast Rhodotorula muciloginosa biomass, termed biosorption, was studied with respect to pH, initial dye concentration and initial metal ion concentration. The biomass exhibited maximum dye and chromium(VI) uptake at pH 5 and pH 6 for nickel(II) in media containing 50 mg/L heavy metal and 50 mg/L remazol blue. It was found that the highest chromium(VI) removal yields measured were 31.3% for 49.0 mg/l initial chromium(VI) concentrations. The nickel(II) removal yield was 32.5% for 22.3 mg/L. Higher R. Blue removal yields were obtained, such as 77.1% for 117.5 mg/L. The maximum dye biosorption yield was investigated in medium with a constant dye (∼50 mg/L) and increasing heavy metal concentration. In the medium with 48.8, 103.8 and 151.8 mg/L chromium(VI) and constant dye concentration, the maximum chromium(VI) biosorption was 7.4, 9.3 and 17.1%, whereas the maximum dye biosorption was 61.6, 56.6 and 55.9%. The maximum nickel(II) biosorptions in the medium with dye were 38.1, 22.1 and 8.8% at 23.7, 37.7 and 60.1 mg/L nickel(II) concentrations. In these media, dye biosorptions were 93.9, 86.4 and 93.3%, respectively.

Biosorption of lead (II) and cadmium (II) using Escherichia coli genetically engineered with mice metallothionein I

2011 ◽  
Vol 63 (8) ◽  
pp. 1607-1613 ◽  
Author(s):  
Verónica Almaguer-Cantú ◽  
Lilia H. Morales-Ramos ◽  
Isaías Balderas-Rentería
Keyword(s):  
Organometallic Compounds ◽  
Freundlich Isotherm ◽  
Genetically Engineered ◽  
Adsorption Model ◽  
Experiment Data ◽  
Suitable Material ◽  
E Coli ◽  
Langmuir Adsorption ◽  
Metal Biosorption ◽  
Treating Effluent

Contamination caused by heavy metals in wastewater has a high potential of risk because they easily penetrate in to the trofic chain accumulating as organometallic compounds. In this work, the expression of mice metallothionein in E. coli (pMt-Thio) was examined as a strategy to enhance metal biosorption efficiency of bacterial biosorbents for Pb(II) and Cd(II) ions. The results showed that pMt-Thio led to significant increase in overall biosorption capacity, especially for biosorption of Pb. Isotherms and kinetic of biosorption were evaluated in this designed system. The influence of metal concentration in solution is discussed in terms of Langmuir and Freundlich isotherm and constants. The Langmuir model was found to correlate better with the experiment data. The biomass showed maximum capacities according to Langmuir adsorption model of 28.14 mgPb/gpMt-Thio and 24.27 mgCd/gpMt-Thio. The study proved that pMt-Thio is a suitable material for the removal of the heavy metal ions studied from aqueous solutions, achieving removal efficiencies higher than 90% for Pb(II) and higher than 40% for Cd(II), and could be considered as a potential material for treating effluent polluted with Cd(II) and Pb(II) ions.

Parameters Affecting Dye Adsorption - Using Graphene Coated Sand (GSC)

2018 ◽  
Vol 4 (02) ◽  
Author(s):  
Seroor Atalah Khaleefa Alia ◽  
Dr. Mohammed Ibrahimb ◽  
Hussein Ali Hussein
Keyword(s):  
Heavy Metals ◽  
Contact Time ◽  
Organic Dyes ◽  
Low Cost ◽  
Dye Adsorption ◽  
Contaminated Water ◽  
Maximum Adsorption Capacity ◽  
Engineering Designs ◽  
Heavy Metals Ions ◽  
Coated Sand

Adsorption is most commonly applied process for the removal of pollutants such as dyes and heavy metals ions from wastewater. The present work talks about preparing graphenic material attached sand grains called graphene sand composite (GSC) by using ordinary sugar as a carbon source. Physical morphology and chemical composition of GSC was examined by using (FTIR, SEM, EDAX and XRD). Efficiency of GSC in the adsorption of organic dyes from water was investigated using reactive green dye with different parameters such as (ph, temperature, contact time and dose). Adsorption isotherm was also studied and the results showed that the maximum adsorption capacity of dye is 28.98 mg/g. This fast, low-cost process can be used to manufacture commercial filters to treat contaminated water using appropriate engineering designs.

Disinfection of Fruits with Activated Water

2019 ◽  
Vol 10 ◽  
pp. 1864-1872
Author(s):  
Prof. Teodora P. Popova
Keyword(s):  
Antimicrobial Activity ◽  
Aqueous Solutions ◽  
Antimicrobial Properties ◽  
The Other ◽  
Gram Negative Bacteria ◽  
High Antimicrobial Activity ◽  
Gram Negative ◽  
E Coli ◽  
Number Of Microorganisms ◽  
Lower Effect

The effect of ionized aqueous solutions (anolytes and catholyte) in the processing of fruits (cherries, morellos, and strawberries) for decontamination has been tested. Freshly prepared analytes and catholyte without the addition of salts were used, as well as stored for 7 months anolytes, prepared with 0.5% NaCl and a combination of 0.5% NaCl and 0.5% Na2CO3. The anolyte prepared with a combination of 0.5% NaCl and 0.5% Na2CO3, as well as the anolyte obtained with 0.5% NaCl, exhibit high antimicrobial activity against the surface microflora of strawberries, cherries, and sour cherries. They inactivate E. coli for 15 minutes. The other species of the fam. Enterobacteriaceae were also affected to the maximum extent, as is the total number of microorganisms, especially in cherries and sour cherries. Even stored for 7 months, they largely retain their antimicrobial properties. Anolyte and catholyte, obtained without the addition of salts, showed a lower effect on the total number of microorganisms, but had a significant effect on Gram-negative bacteria, and especially with regard to the sanitary indicative E. coli.

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