scholarly journals Assessment of genetic purity in hybrid lines by field grow-out test and molecular markers

2016 ◽  
Vol 11 (1) ◽  
pp. 106-118
Author(s):  
VILAS JADHAV ◽  
O.P. VERMA
Keyword(s):  
Molecular Markers ◽  
Genetic Purity ◽  
Hybrid Lines

Assessment of genetic purity of tomato (Lycopersicon esculentum L.) hybrid using molecular markers

2007 ◽  
Vol 115 (1) ◽  
pp. 7-12 ◽  
Author(s):  
Li-Wang Liu ◽  
Yan Wang ◽  
Yi-Qin Gong ◽  
Tong-Min Zhao ◽  
Guang Liu ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Lycopersicon Esculentum ◽  
Genetic Purity

scholarly journals Molecular fingerprinting of lal teer chili variety “morich super f1”

2019 ◽  
Author(s):  
Hossain Sohrawardy ◽  
Salina Sultana
Keyword(s):  
Molecular Markers ◽  
Dna Fingerprinting ◽  
Plant Species ◽  
Polymorphic Band ◽  
Molecular Fingerprinting ◽  
Genetic Purity ◽  
Seed Industry

Abstract Background : Molecular markers do not influence the environment and age of the plant species. DNA fingerprinting is a useful tool for varietal protection for the seed industry. Molecular fingerprinting helps to maintain the genetic purity of parent lines and hybrids. Result: Primer PP 24 and PP 53 showed polymorphic band to Morich Super F 1 and its parents. Conclusion: Morich Super hybridity test conducts at the Lal Teer Biotech Laboratory using these primers.

scholarly journals Insights into Marker Assisted Selection and Its Applications in Plant Breeding

2020 ◽  
Author(s):  
Gayatri Kumawat ◽  
Chander Kanta Kumawat ◽  
Kailash Chandra ◽  
Saurabh Pandey ◽  
Subhash Chand ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Plant Breeding ◽  
Short Duration ◽  
Marker Assisted Selection ◽  
Production Systems ◽  
Crop Improvement ◽  
Gene Pyramiding ◽  
Genetic Purity ◽  
Crop Species ◽  
Germplasm Diversity

Burgeoning the human population with its required food demand created a burden on ever-decreasing cultivated land and our food production systems. This situation prompted plant scientists to breed crops in a short duration with specific traits. Marker-assisted selection (MAS) has emerged as a potential tool to achieve desirable results in plants with the help of molecular markers and improves the traits of interest in a short duration. The MAS has comprehensively been used in plant breeding to characterize germplasm, diversity analysis, trait stacking, gene pyramiding, multi-trait introgression, and genetic purity of different cereals, pulses, oilseeds, and fiber crops, etc. Mapping studies pointed out several marker-trait associations from different crop species, which specifies the potential application of MAS in accelerating crop improvement. This chapter presents an overview of molecular markers, their genesis, and potential use in plant breeding.

scholarly journals Isolation and Identification of Molecular Markers for Fingerprinting of Chilli Hybrids & its Parental Lines

2020 ◽  
Vol 11 (1) ◽  
pp. 713-716
Author(s):  
Shishir Tiwari ◽  
Shweta Sao ◽  
Antu Kurrey ◽  
Pulak Das
Keyword(s):  
Molecular Markers ◽  
Issr Marker ◽  
Genetic Purity ◽  
Isolation And Identification ◽  
Rapd Primer ◽  
Purity Analysis ◽  
Hybrid Seeds ◽  
Environmentally Sensitive ◽  
Parental Lines ◽  
Morphological Differences

Chilli (Capsicum annum) is the predominant sp., which is cultivated in both hot and sweet papers. The maintenance of the genetic purity of chilli plant is a matter of great concern for the breeders. For genetic purity analysis, between true hybrids and off-types, breeders find out morphological differences between them, but this technique is cannot be recognized easily and also costly, tedious to score, and environmentally sensitive. Alternatively,  molecular markers based genetic purity analysis can be employed. The molecular marker-based technique was thus used to overcome the conventional method drawbacks. The main objective of the study is to identify informative molecular markers (ISSR and RAPD) capable of distinguishing Chilli hybrids and their parental lines and their utilization in seed purity assessment. Five parental lines of Chilli (i.eCH10, CH12, CH530, CH709, CH734) were used for the production of 3 hybrids. Total 30 ISSR and 8 RAPD primers were selected for the study of 5 parental lines, among them 2ISSR and 1 RAPD primers produced unique fingerprinting across the hybrids. The ISSR marker UBC815 amplified alleles specific to different parental lines(CH10 & CH12) for hybrids (ACH112), The ISSR marker UBC 827, amplified alleles specific to different parental lines(CH709 & CH12) for hybrids (ACH179). Likewise, RAPD primer B20 for hybrid ACH 753 and their parental lines(CH734 & CH530). Thus, the above study showed that the aid of molecular markers is more reliable, highly efficient, and reproducible for assessing fingerprinting of Chilli commercial hybrid seeds with more accuracy.

scholarly journals Analysis of the relationship between coefficients of relatedness and molecular similarity of parental forms with respect to the heterosis effect in maize

2013 ◽  
Vol 50 (1) ◽  
pp. 39-52
Author(s):  
Agnieszka Tomkowiak ◽  
Zbigniew Broda ◽  
Krzysztof Moliński ◽  
Marta Molińska-Glura ◽  
Józef Adamczyk
Keyword(s):  
Molecular Markers ◽  
Molecular Similarity ◽  
Pedigree Analysis ◽  
Molecular Techniques ◽  
Pedigree Information ◽  
Genetic Purity ◽  
Heterotic Groups ◽  
Heterosis Effect ◽  
Selection Of

SUMMARY Within the last twenty years studies have been conducted at many research centers with the aim of dividing breeding materials into heterotic groups based on molecular markers. Molecular techniques make it possible to study the genetic purity of inbred lines, determine their genetic variability and classify breeding materials for which no information is available on their origin. This study aims to investigate relationships between coefficients of relatedness (pedigree analysis) and molecular similarity estimated on the basis of AFLP and RAPD molecular markers, between parental forms of F1 maize hybrids. Determination of these relationships will make it possible to establish a hierarchy of importance for applied methods concerning selection of parental components for heterotic crossings based on the degree of relatedness and genetic similarity. As a result of the experiment it was shown that in the case of incomplete pedigree information, in the selection of parental components for crossings we may use information concerning molecular similarity determined using AFLP markers and the Jaccard index, which to the least degree differentiates matrices of AFLP and RAPD molecular similarity.

Genetic purity testing of F1 hybrid seed with molecular markers in cabbage (Brassica oleracea var. capitata)

2013 ◽  
Vol 155 ◽  
pp. 92-96 ◽  
Author(s):  
Shan Ye ◽  
Yan Wang ◽  
Danqiong Huang ◽  
Jianbin Li ◽  
Yiqin Gong ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Brassica Oleracea ◽  
Hybrid Seed ◽  
Genetic Purity ◽  
F1 Hybrid ◽  
Purity Testing

scholarly journals Fingerprinting and genetic purity assessment of F1 barley hybrids and their salt-tolerant parental lines using nSSR molecular markers

3 Biotech ◽  
2018 ◽  
Vol 8 (1) ◽  
Author(s):  
Mériam Ben Romdhane ◽  
Leila Riahi ◽  
Rahma Jardak ◽  
Abdelwahed Ghorbel ◽  
Nejia Zoghlami
Keyword(s):  
Molecular Markers ◽  
Salt Tolerant ◽  
Genetic Purity ◽  
Purity Assessment ◽  
Parental Lines

scholarly journals Genetic Purity of Cacao Criollo from Honduras Is Revealed by SSR Molecular Markers

Agronomy ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 225
Author(s):  
Marlon López ◽  
Massimo Gori ◽  
Lorenzo Bini ◽  
Erick Ordoñez ◽  
Erick Durán ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Ex Situ ◽  
Phenotypic Traits ◽  
Genetic Purity ◽  
Santa Barbara ◽  
Genetic Characteristics ◽  
Pests And Diseases ◽  
Genetic Groups ◽  
Productive Potential ◽  
Small Producers

The cultivation of cacao represents an income option and a source of employment for thousands of small producers in Central America. In Honduras, due to the demand for fine flavor cacao to produce high-quality chocolate, the number of hectares planted is increasing. In addition, cacao clones belonging to the genetic group named Criollo are in great demand since their white beans lack of bitterness and excellent aroma are used in the manufacturing of premium chocolate. Unfortunately, the low resistance to pests and diseases and less productive potential of Criollo cacao leads to the replacement with vigorous new cultivars belonging to the other genetic groups or admixture of them. In this study, 89 samples showing phenotypic traits of Criollo cacao from four regions of Honduras (Copán, Santa Bárbara, Intibucá, and Olancho) were selected to study their genetic purity using 16 SSR molecular markers. The results showed that some samples belong to the Criollo group while other accessions have genetic characteristics of “Trinitario” or other admixtures cacao types. These results confirm the genetic purity of Criollo cacao in Honduras, reaffirming the theory that Mesoamerica is a cacao domestication center and also serves to generate interest in the conservation of this genetic wealth both in-situ and ex-situ.

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