scholarly journals Prevalance and perpetuation of chilli fruit rot pathogen (Colletotrichum spp.)

2018 ◽  
Vol 13 (2) ◽  
pp. 245-248
Author(s):  
Priyanka Shinde ◽  
B.G. Barhate ◽  
K. Greeshma
Keyword(s):  
Fruit Rot ◽  
Colletotrichum Spp ◽  
Chilli Fruit

scholarly journals Efficacy of fish compost on management of chilli fruit rot caused by Colletotrichum capsici

2015 ◽  
Vol 14 (17) ◽  
pp. 1493-1495
Author(s):  
Ann Suji H. ◽  
Suthin Raj T. ◽  
Ann Suji H. ◽  
Suthin Raj T.
Keyword(s):  
Fruit Rot ◽  
Colletotrichum Capsici ◽  
Chilli Fruit

scholarly journals Identifikasi dan Uji Patogenisitas Colletotrichum spp. dari Cabai Merah (Capsicum annuum): Kasus di Kricaan, Magelang, Jawa Tengah

2021 ◽  
Vol 26 (2) ◽  
pp. 243-250
Author(s):  
Noorkomala Sari ◽  
Rina Sri Kasiamdari
Keyword(s):  
Colletotrichum Gloeosporioides ◽  
Pathogenic Fungi ◽  
Fruit Rot ◽  
Red Pepper ◽  
Agricultural Field ◽  
Pathogenic Agent ◽  
Central Java ◽  
Causative Agents ◽  
Colletotrichum Spp ◽  
And Control

The investigation of the agent causing diseases is the first step to determine and control the spread of diseases. Anthracnose causing fruit rot on the red pepper in Indonesia that reduces the yield up to 90%. Colletotrichum is reported as the causative fungal agent of anthracnosis on the red pepper. This study aims to determine the type of Colletotrichum spp. pathogenic fungi on the red pepper collected from Agricultural Field Kricaan, Magelang, Central Java. Methods of identification included macroscopic and microscopic characterization and followed by determining the pathogenicity of the pathogenic agent. In this study, Colletotrichum gloeosporioides and C. acutatum are identified as the causative agents of anthracnose in the red pepper collected from the study location. C. acutatum PC3 strain is identified as the most virulent pathogenic agent. Keywords: anthracnosis, diagnosis, diseases, symptoms, pathogen

scholarly journals Bio-efficacy of different biological control agents for the management of chilli fruit rot/anthracnose disease

2019 ◽  
Vol 33 (2) ◽  
pp. 163-168
Author(s):  
B. L. RAGHUNANDAN ◽  
MAYANK V. PATEL ◽  
NEHA M. PATEL ◽  
D. M. MEHTA ◽  
◽  
...  
Keyword(s):  
Biological Control ◽  
Fruit Rot ◽  
Biological Control Agents ◽  
Anthracnose Disease ◽  
Chilli Fruit

scholarly journals First Report of Anthracnose Fruit Rot Caused by Colletotrichum fioriniae on Litchi in China

Plant Disease ◽  
2020 ◽  
Author(s):  
Jin-Feng Ling ◽  
Aitian Peng ◽  
Zide Jiang ◽  
Pinggen Xi ◽  
Xiaobing Song ◽  
...  
Keyword(s):  
Species Complex ◽  
Morphological Characteristics ◽  
Morphological Characterization ◽  
Fruit Rot ◽  
Single Spore ◽  
Potential Threat ◽  
First Report ◽  
Fruit Cracking ◽  
Pure Cultures ◽  
Colletotrichum Spp

Anthracnose fruit rot of litchi (Litchi chinensis Sonn.), caused by Colletotrichum spp., has been mainly associated with the C. acutatum species complex and C. gloeosporioides species complex (Farr and Rossman 2020). In June 2010, isolates of the C. acutatum species complex were isolated together with the C. gloeosporioides species complex from anthracnose lesions on litchi fruits (cv. Nuomici) obtained from a litchi orchard in Shenzhen (N 22.36°, E 113.58°), China. The symptoms typically appeared as brown lesions up to 25 mm in diameter, causing total fruit rot and sometimes fruit cracking. Based on the number of isolates we collected, the C. acutatum species complex appears less frequently on infected fruit compared to the C. gloeosporioides species complex. Since only the C. gloeosporioides species complex has been reported in China (Qi 2000; Ann et al. 2004), we focused on the C. acutatum species complex in this study. Pure cultures of fungal isolates were obtained by single-spore isolation. The isolate GBLZ10CO-001 was used for morphological characterization, molecular and phylogenetic analysis, and pathogenicity testing. Colonies were cultured on potato dextrose agar (PDA) at 25 ℃ for 7 days, circular, raised, cottony, gray or pale orange, with reverse carmine, and 39.6 to 44.7 mm in diameter. Conidia were 13.5 to 19 × 4 to 6 µm (mean ± SD = 15.9 ± 1.1 × 5.2 ± 0.3 µm, n = 50) in size, hyaline, smooth-walled, aseptate, straight, fusiform to cylindrical with both ends acute. Appressoria were 5.5 to 13.5 × 4.5 to 7.5 µm (mean ± SD = 7.6 ± 1.6 × 6.0 ± 0.7 µm, n = 50) in size, subglobose to elliptical, sometimes clavate or irregular, smooth-walled, with entire edge, sometimes undulate, pale to medium brown. These morphological characteristics were consistent with the descriptions of several Colletotrichum species belonging to the C. acutatum species complex, including C. fioriniae (Shivas and Tan 2009; Damm et al. 2012). For molecular identification, genomic DNA was extracted and the ribosomal internal transcribed spacer (ITS), partial sequences of the β-tubulin (TUB2), actin (ACT), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), chitin synthase 1 (CHS-1), and histone3 (HIS3) genes were amplified and sequenced using the primer pairs ITS4/ITS5, T1/Bt2b, ACT512F/ACT783R, GDF1/GDR1, CHS-79F/CHS-354R, and CYLH3F/CYLH3R, respectively (White et al. 1990; Damm et al. 2012). The resulting sequences were submitted to GenBank (ITS: MN527186, TUB2: MT740310, ACT: MN532321, GAPDH: MN532427, CHS-1: MT740311, HIS3: MT740312). BLAST searches showed 98.70%-100% identity to the sequences of the C. fioriniae ex-holotype culture CBS 128517. The phylogram reconstructed from the combined dataset using MrBayes 3.2.6 (Ronquist et al. 2012) showed that isolate GBLZ10CO-001 clustered with C. fioriniae with high posterior probability. Koch’s postulates were performed in the field to confirm pathogenicity. Isolate GBLZ10CO-001 was grown on PDA (25 ℃ for 7 days) to produce conidia. In June 2014, litchi fruits (cv. Nuomici) were sprayed with conidial suspensions (106 conidia/ml), with sterile water as blank controls, and each treatment inoculated at least 15 fruits. Inoculated fruits were covered by an adhesive-bonded fabric bag until the trial ended. After 31 days, typical symptoms were observed, while control fruits remained asymptomatic. The fungus was re-isolated from diseased fruits and identified as C. fioriniae according to the methods described above. To our knowledge, this is the first report of anthracnose fruit rot on litchi caused by C. fioriniae, one species of the C. acutatum species complex, in China. For the difficulty in distinguishing anthracnose caused by C. fioriniae from the C. gloeosporioides species complex just by the symptoms, and mixed infection usually occurring in the field, further investigations are required to reliably assess the potential threat posed by C. fioriniae for litchi production in China.

scholarly journals Etiology and Population Genetics of Colletotrichum spp. Causing Crown and Fruit Rot of Strawberry

2002 ◽  
Vol 92 (11) ◽  
pp. 1245-1252 ◽  
Author(s):  
A. R. Ureña-Padilla ◽  
S. J. MacKenzie ◽  
B. W. Bowen ◽  
D. E. Legard
Keyword(s):  
Random Amplified Polymorphic Dna ◽  
Crown Rot ◽  
Fruit Rot ◽  
Chain Reaction ◽  
Internal Transcribed Spacer Region ◽  
Low Diversity ◽  
Diseased Fruit ◽  
Colletotrichum Spp ◽  
Polymerase Chain ◽  
Polymerase Chain Reaction Primers

Isolates of Colletotrichum spp. from diseased strawberry fruit and crowns were evaluated to determine their genetic diversity and the etiology of the diseases. Isolates were identified to species using polymerase chain reaction primers for a ribosomal internal transcribed spacer region and their pathogenicity was evaluated in bioassays. Isolates were scored for variation at 40 putative genetic loci with random amplified polymorphic DNA and microsatellite markers. Only C. acutatum was recovered from diseased fruit. Nearly all isolates from crowns were C. gloeosporioides. In crown bioassays, only isolates of C. gloeosporioides from strawberry caused collapse and death of plants. A dendrogram generated from the genetic analysis identified several primary lineages. One lineage included isolates of C. acutatum from fruit and was characterized by low diversity. Another lineage included isolates of C. gloeosporioides from crowns and was highly polymorphic. The isolates from strawberry formed distinctive clusters separate from citrus isolates. Evaluation of linkage disequilibrium among polymorphic loci in isolates of C. gloeosporioides from crowns revealed a low level of disequilibrium as would be expected in sexually recombining populations. These results suggest that epidemics of crown rot are caused by Glomerella cingulata (anamorph C. gloeosporioides) and that epidemics of fruit rot are caused by C. acutatum.

scholarly journals First Report of Diplodia seriata, the Anamorph of “Botryosphaeria” obtusa, Causing Fruit Rot of Olive in Spain

Plant Disease ◽  
2008 ◽  
Vol 92 (2) ◽  
pp. 311-311 ◽  
Author(s):  
J. Moral ◽  
F. Luque ◽  
A. Trapero
Keyword(s):  
Relative Humidity ◽  
Fruit Trees ◽  
Southern Spain ◽  
Fruit Rot ◽  
Tween 20 ◽  
Diplodia Seriata ◽  
First Report ◽  
Morphological Criteria ◽  
Botryosphaeria Obtusa ◽  
Colletotrichum Spp

In October, 2006, 1,000 olive fruits (Olea europaea) were collected to study latent infections of Colletotrichum spp., causal agent of anthracnose, in an olive orchard in Córdoba Province (southern Spain). The fruits were incubated at 22 to 24°C and 100% relative humidity. Thirteen percent of the fruits showed a black rot that covered part or all of the fruit and pycnidia formed on the fruit surface. The fungus most consistently isolated from affected fruits was identified as Diplodia seriata, the anamorph of “Botryosphaeria” obtusa, by morphological criteria (1). Pathogenicity tests were performed on 50 immature and 50 mature fruits of olive cv. Hojiblanca. The fruits were washed in sterilized water with Tween 20 at 20 μl/liter and surface sterilized in 20% sodium hypochlorite for 2 min. Twenty-five of the immature and 25 mature fruits were wounded with a sterile 0.5-mm-diameter needle, inoculated by immersion in aqueous conidial suspensions (2.2 × 105 conidia ml–1) for 30 min. The same number of immature and mature fruits, wounded and unwounded, were treated only with sterilized Tween 20 water and served as the control. All fruit were incubated at 22 to 24°C and 100% relative humidity. The experiment was repeated twice. Eight days after inoculation, 75% of the mature inoculated fruits showed a general rot with pycnidia developing on the surface of the fruit. After 20 days, the first symptoms of disease were observed on immature olive fruits (2%) and all mature fruits. After 50 days, only 14% of immature inoculated fruits exhibited symptoms of the disease. The fungus caused mummification of mature fruits. D. seriata was reisolated from lesions on all infected fruits. There were no differences in the amount and severity of disease between wounded and unwounded fruits. Control fruits did not display disease symptoms after 50 days. Incidence of olive fruits affected by D. seriata in olive orchards in southern Spain is unknown, although it may be low since it was detected only in 1 of 12 olive orchards sampled for Colletotrichum spp. in 2006. Incidence of fruit rot associated with D. seriata in this orchard was 1.2% of fruits, which also were affected by Colletotrichum. D. seriata has a worldwide distribution and infects numerous fruit trees. In our research, D. seriata is pathogenic on olive fruits but weakly virulent. To our knowledge, this is the first report worldwide of the anamorph of “B.” obtusa causing fruit rot on olives. Reference: (1) A. J. L. Phillips et al. Fungal Divers. 25:141, 2007.

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