The 16S rRNA Analysis and Enzyme Screening of Bacillus from Rhizosphere Soil of Lombok Island

Bacillus are commonly found in nature, especially in soil and food. It has the ability to produce bioactive compounds as well as the enzyme. This study was aimed to isolate, identify, and screen their enzyme activities. Four soil samples from Mandalika, Lombok Island, West Nusa Tenggara (NTB), were used for isolation. Bacillus was isolated using the heat-shock method and characterized through Gram staining, endospore staining, and morphological phenotype. Bacillus identification was conducted based on 16S rRNA gene sequence. The hydrolytic enzyme activities were checked qualitatively using selective media, and the enzyme tested including amylase, galactosidase, lipase, protease, and cellulase. As many as twenty-two bacteria isolates were obtained from four soil samples and represented 15 distinct species. The member of bacteria genera successfully identified, consisted of Bacillus sp., Brevibacillus sp., and Fictibacillus sp. Bacillus sp. was the most isolated. Some of the isolated bacteria have the ability to produce lipase, protease, and cellulase that potential to be used in biotechnology processes. Keywords: Bacillus, hydrolytic enzymes, identification, screening

Peningkatan Kadar Zn Beras Pecah-Kulit pada Sistem Penggenangan Berselang Melalui Aplikasi Pupuk Kandang Diperkaya Zn Heptahidrat

Zn levels in rice are essential for maintaining consumer health. However, Zn deficient plants can reduce levels of Zn in grains and the ability of plants to express their genetic potentials. This study examines the use of cow manure enriched with Zn heptahydrate on increasing Zn levels of broken skin rice. The experiment was arranged in a randomized single factor group design with seven levels of manure dose treatment, namely: 0; 2.5; 5, 7.5, 10, 12.5, 15 tons ha-1, with 3 replications. Spacing was 30 cm x 30 cm with a size of 3 m x 4 m. The parameters observed were plant growth, the weight of 1,000-grain, percentage of empty grain, production, Zn and protein contents of the brown rice. The collected data were analyzed using analysis of variance with a confidence interval of 5%, followed by the Duncan test of 5%. The results showed that the application of cow manure with a dose of 10 tons ha-1 enriched by 5 kg ha-1 Zn heptahydrate could increase the number of productive tillers, weight of 1,000-grains, production, Zn and protein contents of the brown rice as well as suppress the grainy rice grain of Mekongga variety of lowland rice on the intermittent flooding. Keywords: brownrice, cow manure, irrigation system, Zn heptahydrate

Strategi Kesiapan Koperasi dalam Digitalisasi Perkebunan Kelapa Sawit Rakyat

KUD Tunas Muda is one of the cooperatives active in using information technology to serve the needs of its members, especially related to oil palm activities. However, not all palm oil activities have been carried out through digitization. This paper aims to provide an overview of the readiness of the KUD located in Riau Province in digitizing smallholder oil palm plantations, which include activities in preparing inputs, cultivation, marketing, and receiving payments. This study also aims to analyze strategic priorities that cooperatives can carry out in digitizing smallholder palm oil. This study uses two methods in its analysis, namely descriptive analysis, and analytical hierarchy process. Based on the study, cooperative management is quite prominent in initiating and building digital systems in services for their members. This is because the KUD already has experience in digitalization, although the function is still limited. According to the analysis, the prioritized strategy is to increase the understanding of oil palm farmers, especially among members, in using digital technology and information to support the digitalization of oil palm plantations. Keywords: analytical hierarchy process, cultivation, information technology, KUD Tunas Muda, smallholder plantation

Phytochemical Screening and Antibacterial Activities of Senggani (Melastoma malabathricum L.) Ethanolic Extract Leaves

The aim of the current research was to perform phytochemical screening and to know the pathogenic antibacterial activities of senggani leaves extract. Phytochemical screening was done by testing eight active compounds. Antibacterial activities testing was done by using well diffusion method at concentration level of 25%, 50%, 75%, 100%. Positive control was amoxicillin, while the negative control was aquadest. Meanwhile, pathogenic bacteria were Escherichia coli, Shigella dysenteriae, Staphylococcus aureus, and Pseudomonas aeruginosa. Research design applied was complete random design, in which the data collected were analyzed using one-way ANOVA, continued by post-hoc test through Tukey method to know which concentration provide the most significant difference. Research results showed that ethanolic extract of senggani leaves has eight active compounds, those are phenolics, alkaloids, flavonoids, tannins, triterpenoids, glycosides, steroids, and saponins. This further proved that the ethanolic extract of senggani leaves have antibacterial activity and are able to inhibit the growth of all bacteria tested. The best ability shown to inhibit E. coli bacteria was at concentration of 100%, Sh. dysenteriae and P. aeruginosa started at the concentration of 75%, and S. aureus started at the concentration of 50%. Keywords: active compounds, bacteria growth, inhibition, well diffusion

Sistem Pengembangan Asuransi Usaha Ternak Sapi/Kerbau di Provinsi Aceh

Cow/buffalo-business insurance (AUTS/K) is a form of risk mitigation for livestock businesses run by farmers. Various risks that are often occurred include livestock death, natural disasters, disease outbreaks, and livestock theft. If not handled properly, it can threaten the sustainability of this business, especially the small-scale livestock-business sector. This study aimed to formulate a developing system of cattle/buffalo-livestock insurance using the soft-systems methodology (SSM) approach. This research was conducted in Aceh Province from December 2020 to February 2021 using an expert survey by collecting data and information through parties who have competence or expertise in the field being studied. The development of this insurance system can be said to be an effort to improve and a solution to any problems currently being experienced in the implementation of AUTS/K. The result of improvements includes optimizing the roles of each party involved and program innovation to intensify AUTS/K products among breeders. The results showed that the SSM approach could describe the problem well and form a conceptual model for the AUTS/K system in Aceh province. Improvements to policies, assistance, and promotion systems are recommended as solutions to the problems related to livestock-insurance system implemented. Keywords: breeders, conceptual model, insurance system, soft-systems methodology, syariah insurance

Keanekaragaman Serangga Fitofag pada Sengon Falcataria moluccana (Miq.) Barneby & J.W. Grimes dari Jawa dan Hawaii di Persemaian di Bogor

Albizia (Falcataria moluccana) cultivated in Indonesia for timber plantations can increase the farmer’s income, even though albizia became an invasive alien species that can cause the economic and ecological losses. The controlling of invasive species using biological control is an effective method. The purpose of this study was to determine the phytophagous insects on albizia from Java and Hawaii in seedling in Bogor. The experiment was conducted in Carangpulang, Dramaga, Bogor. The experiment was arranged in a completely randomized block design using seed origin as a treatment (albizia Java and Hawaii) with 4 replicates. Total number of tree sample was 160 trees. Observations were conducted every 2 weeks until 32 weeks after planting. Identification of insects was conducted in the Laboratory of Insect Biosystematics, IPB. A total of 67 phytophagous insects were collected, 57 species in albizia from Java and 54 species in albizia from Hawaii. There was no difference in abundance and diversity of phytophagous insects on albizia from Java and Hawaii. Larvae of Eurema blanda (Lepidoptera: Pieridae) became an important pest causes leaf defoliation. Furthermore, Hulodes caranea (Lepidoptera: Noctuidae), Margarodes sp. (Hemiptera: Margarodidae), Choristoneura sp. (Lepidoptera: Tortricidae), and Adoxophyes sp. (Lepidoptera: Tortricidae) identified as important pests that have not been reported in the other research. Keywords: albizia, defoliator insect, Fabaceae, important pests, invasive alien species

Aplikasi Dosis Vermikompos dan Urea Pada Tanaman Kedelai Varietas Anjasmoro di Tanah Berpasir

The low area for soybean plantation is still a major problem in meeting soybean needs. One of the efforts to increase national soybean production is by expanding the planting area by utilizing the sandy soil area that is still not used for soybean cultivation. This research was conducted from September-November 2020 in a greenhouse of the Agronomy Laboratory, Bengkulu University. This study aims to obtain the best dose of urea and vermicompost for the growth and yield of soybean in sandy soil. The research design used a completely randomized block design with two factors and 3 replications. The first factor was the dose of urea consisting of four levels, namely U0 = 0 kg ha-1, U1 = 50 kg ha-1, U2 = 100 kg ha-1, and U3 = 150 kg ha-1. The second factor was the dose of vermicompost, namely K0 = 0 ton ha-1, K1 = 5 tons ha-1, K2 = 10 ton ha-1, and K3 = 15 ton ha-1. The measured variables were the plant growth and the yield. Soil and vermicompost analyses were carried out before the study. The results showed that there was an interaction between the vermicompost and the urea dosages. The 100 kg ha-1 urea and the 10 ton ha-1 vermicompost treatment gave the highest plant height. The 150 kg ha-1 urea dan the 15 ton ha-1 vermicompost treatment formed the most number of branches. The highest soybean yield was given by 15 ton ha-1 vermicompost and 50 kg ha-1 urea to the total pod and the seed weight. Keywords: sandy soil, soybean, urea, vermicompost

Status Daya Dukung Lahan untuk Keberlanjutan Pangan di Kabupaten Klungkung

Land is an essential component for the sustainability of human life. Population growth and urbanization affect the availability of land in an area. Therefore, the land never increases, but the need for land continues to grow every year, converted to housing and other facilities. As an area that continues to develop, Klungkung Regency has decreased the availability of land due to development and investment activities, especially tourism investment and the construction of other supporting facilities for tourism and regional development. Therefore, the availability of land in Klungkung Regency continues to experience pressure, which impacts the ability to provide food. This study aims to determine land requirements, land availability, and land carrying capacity status in Klungkung Regency, which the policy-makers can consider to support food sustainability in Klungkung Regency. This study uses a quantitative approach using primary data from direct observations in the field and secondary data from government and previous research. The results show that the carrying capacity of land in the Klungkung Regency is in deficit based on the projections for 2021 to 2030. In overcoming the land deficit, it is necessary to increase land productivity by using land effectively and technology transfer to increase food productivity. Keywords: carrying capacity, food sustainability, Klungkung Regency land conversion

Ethnobotany of Lontar (Borassus flabellifer L.) in Tuamese Village, East Nusa Tenggara

Lontar is a plant that distributed in tropical areas and has beneficial values for the people in Tuamese Village, Biboki Anleu District, North Central Timor Regency. However, the information about the ethnobotany of lontar is not documented. This research aimed to determine the utilization of lontar in Tuamese Village. Data were collected using semi-structured interview techniques and field observation. The ethnobotany data were analyzed to calculate cultural significance index value. The results showed that the people in Tuamese Village who generally work as lontar farmers use stem, leaves, flowers, and fruit as building and fence materials, handicraft materials, food and drink ingredients, and firewood. Product from sap flower, namely liquid sugar, slab sugar, and traditional fermented drink (sopi) are the leading trade commodity. The utilization of lontar in Tuamese Village is grouped into seven forms of utilization with index of cultural significance 151 and classified very high. The classification means that lontar greatly affects people's lives in Tuamese Village and its use has become cultured. People's dependence on lontar is very high so the conservation effort needs to make the existence of lontar is maintained. Keywords: index cultural significance, lontar, Tuamese

Sikap dan Tingkat Kepuasan Petani akan Introduksi Varietas Unggul Baru Padi Gogo

The development of rice irrigated farming is constrained by the conversion of land agricultural to non-agricultural. Therefore, policy to develop upland rice production in dryland and rainfed lowland is increasingly being implemented. Efforts to expand the application of technological innovations as a lever of upland rice production have intensified by introducing new superior varieties of upland rice. This research aims to analyze farmers' attitudes and satisfaction levels with introducing upland rice to attributes based. The location of this research is in Tegalgiri Village, Nogosari District, Boyolali Regency. Primary data were obtained through a survey method of 35 farmers who planted the Rindang 1, Rindang 2, Inpago 10, and Inpago 12 varieties. Farmer attitudes were analyzed using the Fishbein Multi-attribute Model, while farmer satisfaction level was analyzed using the customer satisfaction index (CSI). The results are the attributes "production" and "resistance to pests and diseases" were agronomic performance which was the most essential and primary consideration for farmers in choosing varieties. Farmers have a positive attitude towards the four new superior varieties. The meaning is that farmers want to adopt and plant the new superior varieties of upland rice. Farmers' satisfaction levels are in the "satisfied" to "very satisfied" category of the four new superior varieties. Hopefully, the new superior upland rice varieties that were introduced can be a choice of varieties by farmers. Keywords: atributtes, farmer’s attitude, satisfaction level, upland rice, superior varieties