scholarly journals Incidence and hormonal stimulation of cows with ovarian hypotrophy

Author(s):  
Daniel BEREAN ◽  
Liviu Marian BOGDAN ◽  
Ileana BOGDAN ◽  
Anamaria BLAGA PETREAN ◽  
Mihai CENARIU ◽  
...  

The purpose of this research was to establish the incidence and to compare the efficiency of two products used for the hormonal stimulation of ovarian hypotrophy. Gynaecological investigations were performed in 2 farms. The animals with ovarian hypotrophy (ovaries smaller than normal and with a slippery surface) were divided in 2 equal groups depending on the protocol applied: Progesterone (Prid Delta, CEVA) and Progesterone (Dehydroxyprogesterone 10%, Faculty of Veterinary Medicine Cluj-Napoca). In case of group 1 the treatment was performed with Prid Delta (CEVA), vaginal use, 1.55 g of progesterone/animal for 7 days. For the animals of group 2 the treatment was performed with Dehydroxyprogesterone, subcutaneous injection, 1.5 g of dehydroxyprogesterone/animal for 7 days. For each group were observed: the estrous signals, the interval of estrous apparition, the number of gestation obtained after the first artificial insemination.

2015 ◽  
Vol 27 (1) ◽  
pp. 263 ◽  
Author(s):  
R. H. Alvarez ◽  
F. L. N. Natal ◽  
R. M. L. Pires ◽  
K. M. R. Duarte ◽  
C. A. Oliveira

The injection of a low dose of eCG has the potential to induce multiple ovulation and pregnancies in cattle. The present study aimed to evaluate the ovarian response, conception rate and incidence of twin pregnancies of cyclic cows receiving 1 of 2 low doses of eCG. Multiparous Nellore (Bos t. indicus) cows with plasma progesterone levels >1 ng∙mL–1 on at least one of 2 blood samples collected at 10-day intervals (Day –10 and Day 0) received an intramuscular (IM) injection of 2 mg of oestradiol benzoate (EB; Estrogin®, AUSA, São Paulo, Brazil) and a vaginal device (DIP) containing 1 g of progesterone (Primer®, Tecnopec, São Paulo, SP, Brazil) on Day 0. On Day 8, the DIP was removed and cows received an IM injection of 150 μg of cloprostenol (Veteglan®, Hertape Calier, Juatuba, MG, Brazil). At this time, the animals were randomly distributed into 3 groups. Group 1 (n = 30) received an IM injection of 2 mL of saline, whereas groups 2 (n = 41) and 3 (n = 23) received 600 IU and 900 IU of eCG (Novormon® MSD Saude Animal, São Paulo, Brazil), respectively. Twenty-four hours later (Day 9), all groups received 1 mg of EB and were submitted to fixed-time artificial insemination (FTAI) 30 h later (i.e. 54 h after DIP removal). Oestrus observation was performed daily from the time of the withdrawal of the DIP until the day of FTAI. Ovaries were examined ultrasonically at the time of FTAI, the following day and 7 days after FTAI. Pregnancy diagnosis was done by ultrasonography 30 days after FTAI and the incidence of twin or single calves was recorded at birth. Data were analysed by chi-square test. The rate of expression of oestrus was 70.0% (group 1), 82.9% (group 2), and 78.2% (group 3; P = 0.25). Cows that had 2 or more large follicles at the time of FTAI was 0% (group 1), 14.6% (group 2), and 34.8% (group 3; P < 0.05). The ovulation rate of cows in group 1 (80.0%) was higher than cows in groups 2 (48.8%) and 3 (52.2%; P < 0.05). The conception rates for groups 1, 2, and 3 were 50.0, 26.8, and 39.1%, respectively (P < 0.05). Two animals in group 3, one in group 2, and none of group 1 had twin pregnancies on Day 30 after FTAI. Only one of these cows (group 3) had a twin calving. It was concluded that the injection of 600 or 900 IU eCG, in an oestradiol/progestogen FTAI protocol does not result in an increase in the rate of twin calvings, but may negatively affect pregnancy rates of cyclic Nellore cows.Financial support was provided by FAPESP (proc. 2011/13096–0).


1975 ◽  
Vol 53 (2) ◽  
pp. 171-173 ◽  
Author(s):  
Thomas M. Trottier ◽  
John B. Armstrong

A procedure has been developed for artificial insemination of Ambystoma mexicanum that involved hormonal stimulation of both male and female. Ovulation was induced by follicle-stimulating hormone (FSH), and usually began 18–24 h after the injection. The male was injected with chorionic gonadotropin at the time the female was given FSH. At the time of ovulation, the male was placed with a "dummy" female and spermatophores were produced 10–20 min later. These spermatophores were collected and placed in amphibian culture medium at 4 °C. Sperm remained motile for up to 8 h. About 50% fertilization was obtained with sperm produced in this manner, compared with about 60% in natural inseminations.


2011 ◽  
Vol 14 (2) ◽  
pp. 66-68
Author(s):  
Natalya Alexandrovna Gavrilova ◽  
Olga Evgen'evna Tishchenko

Aim. To study effect of sulodexide (Vessel Due F) on the functional state of endothelium in patients with diabetic retinopathy. Materials and methods. A total of 37 patients with DR were divided in 2 groups and treated with sulodexide. Group 1 comprised 16 patients withnon-proliferative DR, group 2 included 21 patients with preproliferative DR. The functional state of endothelium was estimated from the plasma andserum levels of endothelial factors (sVCAM, endothelin, nitric oxide, t-PA, Willebrand factor). Results. The measurement of the initial levels of endothelial factors in both groups revealed significant changes in endothelin, nitric oxide, and sVCAMsuggesting disturbances of endothelial function due to DR. Sulodexide therapy normalized it regardless of DR stage and thereby improved functionalactivity of retina. Conclusion. This study has demonstrated beneficial effect of sulodexide on endothelial function in patients with DR due to correction of the productionof vasoactive factors (endothelin, nitric oxide) and stimulation of fibrinolytic activity of the vascular wall (t-PA).


Author(s):  
Mihai CENARIU ◽  
Emanuel JOSPE

The aims of this study were to identify the status of repeat breeding syndrome (RBS) in a dairy farm, to establish the main cause of RBS and to reduce the number of RBS using the Ovsynch protocol. 20 cows were diagnosed with RBS using computer analysis of the herd and a specific diagnosis for the cause of RBS was attempted. Subsequently, they were divided into 2 groups: cows in group 1 (n=10) received no hormonal treatment while those in group 2 (n=10) were treated using the Ovsynch protocol. Artificial insemination and pregnancy diagnosis was performed in all cows. The 70% pregnancy rate obtained for Ovsynch showed that the hormonal effect as well as correct timing of insemination had a significant influence on RBS, pointing out towards the managerial factor as one of the main causes of RBS. The cow factor was also included, as endometritis, follicular cysts and anovulatory heats were diagnosed.


2018 ◽  
Vol 69 (1) ◽  
pp. 801 ◽  
Author(s):  
I. DOGAN ◽  
Z. NUR ◽  
B. KILINC

The efficiency of medroxyprogesterone acetate (MAP) sponges or norgestomet ear implants (half or entire) for synchronizing and inducing the estrous cycle in non-lactating Kivircik ewes was investigated during the natural non-breeding period. Ewes were treated for 11 days either with 60 mg MAP sponges (group 1, n=27) or with 1.5 mg norgestomet (group 2, n=25) or with 3 mg norgestomet (group 3, n=27) ear implants. In addition, each ewe received an intramuscular injection of 500 IU of equine chorionic gonadotropin (eCG) and 125 μg cloprostenol (PGF2α), 48 h prior to progestagen removal. Double Cervical Artificial Insemination (AI) with diluted fresh semen was performed at a fixed time (36 and 48 h) following progestagen withdrawal. Mean values for estrous detection rates at the first 12 ± 6 h and within 72 h, the time from progestagen removal to the onset of estrous, the duration of the induced estrous and pregnancy rate were found to be 46.8%, 86.1%, 26.1 ± 7.3 h, 27.0 ± 10.7 h and 27.8%, respectively. There were significant differences between groups 2 and 3 in the time of induced estrous onset (P<0.05). These results indicate that, each of the three protocols was equally efficient in inducing and synchronizing estrus in non-lactating Kivircik ewes during the natural non-breeding period.


PEDIATRICS ◽  
1985 ◽  
Vol 75 (5) ◽  
pp. 855-858
Author(s):  
Masato Mitsuhashi ◽  
Hiroyuki Mochizuki ◽  
Kenichi Tokuyama ◽  
Akihiro Morikawa ◽  
Takayoshi Kuroume

The hyperresponsiveness of cough receptors was evaluated using the acetic acid inhalation test in healthy adults, patients with bronchial asthma, and children with or without cough. The concentration of acetic acid inducing cough was more than 20% in all 16 healthy adults and 18 children in the control group. There were two groups of asthmatic patients: Those in group 1 showed normal response to more than 20% acetic acid (n = 46), and those in group 2 showed a sensitive reaction to less than 10% (n = 11). Mean age was 9.0 ± 4.2 years in group 1 and 15.1 ± 7.6 years in group 2 (statistical significance, P &lt; .001). Six of 11 asthmatic patients in group 2 were classified as nonallergic asthmatics, whereas only five of 46 patients in group 1 were nonallergic (P &lt; .01). Bronchoconstriction was not induced in any case, in spite of the production of cough. It is suggested that the hyperresponsiveness of individual cough receptors without the stimulation of irritant receptors be evaluated.


Author(s):  
Daniel BEREAN ◽  
Liviu Marian BOGDAN ◽  
Ileana BOGDAN ◽  
Ovidiu GIURGIU ◽  
Anamaria Blaga PETREAN ◽  
...  

The purpose of this research was to evaluate a product developed at the Faculty of Veterinary Medicine of Cluj Napoca and his efficiency in the control of the puerperium at cows. The study was carried out between January 2017 to December 2018 in a dairy farm from Mures county, Romania. In this study were enclosed 60 cows, randomly divided in 3 groups: Puerperal group (group 1), Puerperal and Estrumate group (group 2), and control group (group 3). For each group the treatment applied for the control of puerperium was different. For each group were followed: the evolution of puerperium, the appearance of the first postpartum estrous cycle, the interval of service period and the number of artificial inseminations necessary for one gestation. For the puerperium control and to avoid the postpartum complications Puerperal intrauterine suspension it’s a good option.


2020 ◽  
Vol 99 (10) ◽  
pp. 1073-1078
Author(s):  
Larisa S. Vasileva ◽  
Dina V. Rusanova ◽  
Natalya V. Slivnitsyna ◽  
Oleg L. Lakhman

Introduction. Objective data necessary for acquiring information about the localization and severity of the pathological process in afferent conductive structures can be obtained by registering somatosensory evoked potentials (SSEP). The goal is to identify neurophysiological features during the registration of SSEPs during stimulation of the median and tibial nerves in patients with the vibrational disease (VD). Material and methods. 140 people were examined: group 1 - 50 patients with VD associated with the combined effects of local and general vibration (aged 48.7 ± 3.1 years); Group 2 - 50 patients with VD associated with exposure to local vibration (aged 48.9 ± 2.8 years); Group 3 - control, including 40 relatively healthy men without the exposure to vibration (aged 49.1 ± 2.5 years). Registration of SSEP was carried out with stimulation of the median and tibial nerves. Statistical processing of the results is “Statistica 10.0” (StatSoft, USA, 2011). The differences in the mean values in the samples were estimated using the Wilcoxon criterion. The differences were considered statistically significant at p <0.05 and 0.01. Results. When the median nerve was stimulated, the latency of the SSVP components increased: for group 1 - indices N9, N10, N11, N13, N20, P25, N30, for group 2 - components N10, N11, N13, N30. A marked increase in latency and intervals was found in group 1. When the tibial nerve was stimulated in group 1, a statistically significant increase in the latency of components N18, N22, N30, characterizing the conduction from the popliteal fossa to the lumbar and then to the cervical level of the spine was revealed. Discussion. The results of the study are consistent with data from previous studies on SSEPs with stimulation of the median nerve. For the first time, it was revealed that in patients of group 1, the registration of SSEP during stimulation of the tibial nerve gives an increase in the time of the impulse at the peripheral level in the popliteal fossa. Conclusion. The SSVP registration method expands the state of afferent pathways. Changes in the state of neurons of the central afferent conducting structures in patients of groups 1 and 2 consisted of more pronounced functional changes at the level of the cervical spinal cord and somatosensory cortex in group 1. During stimulation of the tibial nerve, a slowing down of the afferent excitation wave from the distal sections and further along the ascending paths of the spinal cord to the posterior pillars of the cervical region was noted.


Animals ◽  
2020 ◽  
Vol 10 (2) ◽  
pp. 306 ◽  
Author(s):  
Lima Rodrigues ◽  
Rocio Amezcua ◽  
Glen Cassar ◽  
Terri L O’Sullivan ◽  
Robert Friendship

In order to efficiently have a consistent supply of service-ready gilts available to incorporate into each batch of breeding sows, it is necessary to manipulate the timing of estrus and possibly the timing of ovulation of gilts. Estrus can be synchronized by the withdrawal of altrenogest after at least 14 days of treatment. It is possible that protocols developed to induce ovulation, and therefore allow fixed-time artificial insemination (FTAI), can improve the predictability of gilt breeding. This study investigated the effect of two FTAI protocols in gilts on reproductive performance and timing of farrowing and piglet weaning weight compared to gilts bred based on signs of estrus after cessation of altrenogest. Puberty was induced in gilts, followed by treatment with altrenogest. Following altrenogest withdrawal, 180 gilts were assigned to one of three treatment groups. Group 1 gilts (LUT, n = 62) were treated with 600 IU equine chorionic gonadotropin 24 h after altrenogest withdrawal and 5 mg porcine luteinizing hormone (pLH) 80 h later, followed by a single FTAI 36 h after pLH. Group 2 gilts (TRI, n= 61) received 2 mL of a gonadotropin-releasing hormone agonist, triptorelin acetate, intravaginally 6 d after altrenogest withdrawal and were bred by a single FTAI 24 h later. Group 3 gilts (CON, n = 57) were observed for estrus and bred twice by AI, 24 h apart. LUT and TRI gilts farrowed closer together (2.4 ± 1.6 and 2.9 ± 1.2 d(days), respectively) compared to CON gilts (4.5 ± 3.3 d). Piglets in LUT were 80 g (p < 0.001) heavier and piglets in TRI were 64 g (p < 0.05) heavier at weaning than CON piglets, when controlling for birth weight. Results indicate that FTAI might be useful as a means of minimizing the time from the first to the last gilt farrowing in a breeding batch of gilts. However, modifications of the protocols may be required to ensure optimum farrowing rates and litter size.


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