scholarly journals Determination of the relatedness, ease of hybridization and gene exchange among members of the genus Capsicum in Nigeria

2020 ◽  
Vol 12 (3) ◽  
pp. 658-672
Author(s):  
Paul B. AHMODU ◽  
Joseph A. MORAKINYO ◽  
Catherine T. AHMODU
Keyword(s):  
Related Species ◽  
Maternal Inheritance ◽  
Interspecific Crosses ◽  
Gene Exchange ◽  
Closely Related Species ◽  
Partial Dominance ◽  
Leaf Density ◽  
Genus Capsicum ◽  
Flower Position

The fruit of Capsicum plants have a variety of names depending on place and type. This research was focused at studying the exchange of genes amongst members of the genus Capsicum in Nigeria. The process of interspecific and intervarietal hybridization of two (2) species of the genus Capsicum; C. annuum and C. frutasense were carried out and the results show that most of the studied attributes like arrangement of leaves, shape of leaves, leaf density were undetermined while majority with distinct changes follow maternal inheritance. There were less successes among the interspecific crosses and high successes between varietal crosses ranging from 19.5% to 2.4% for the inter-varietal cross and 9.3% to 2.4% for interspecific crosses. This indicates that there are more hybridization barriers among interspecific hybridization than inter-varietal hybridization. The closely related species and varieties had higher percentage success of hybridization and vice versa. Characters such as Hypocotyl colour had partial dominance. Erect flower position is dominant in W and hybrid T*W while pendant is recessive. Red fruit colour at maturity, green fruit stalk colour was dominant while yellow was recessive. A good knowledge of how related species of the genus Capsicum are, the easier it will enable researchers to hybridize and improve the genus.

scholarly journals Scientific-methodological approach for standardization of herbal raw materials and herbal preparations using high-performance thin-layer chromatography procedure

2021 ◽  
pp. 76-89
Author(s):  
К. О. Хохлова
Keyword(s):  
Quality Control ◽  
Thin Layer ◽  
Related Species ◽  
High Performance ◽  
Raw Materials ◽  
Methodological Approach ◽  
Herbal Drugs ◽  
Closely Related Species ◽  
Layer Chromatography

The high-performance thin-layer chromatography (HPTLC) is widely used for quality control of herbal raw materials (HRM). The purpose of this work were to define the problematic issues of quality control of herbal drugs in Ukraine and to develop a systematic scientific and methodological approach for the standardization of HRM and herbal drugs using the modern analytical technique of HPTLC: to optimize the existing and develop new methods of identification and determination of adulterations, and to develop alternative methods of quantitative determination, as well as studying of the stability. As a result of a critical evaluation of existing approaches for the standardization of HRM and herbal drugs of Ukraine’s flora, numerous problematic issues were identified, including the existence of a limited number of national monographs / national parts of monographs on the HRM of the Ukraine’s flora, which were not previously standardized by other pharmacopeias; non-consideration of Ukraine’s flora species (non-pharmacopoeial) in monographs on closely related species that introduced in the State Pharmacopoeia of Ukraine; lack of specificity of combinations of physical and chemical methods used for quality control; lack of specificity and reproducibility, the labor intensity of methods; necessity of usage of toxic solvents and precursors; use of different groups of markers and methods for the HRM and its finished product or HRM and its closely related species, etc. The proposed systematic scientific and methodological approach includes: a preliminary collection of information about the object of study, markers/groups of bioactive substances, approaches to its standardization; theoretical evaluation of existing techniques of quality control in monographs on HRM and experimental approbation using samples of domestic plants, determination of the need for optimization of existed methods by the parallel development of specific, reproducible and documented methods for identification, adulterations’ detection, quantification, and bio-detection (if necessary) by HPTLC method. The approach is illustrated with examples. The proposed approach can be used for comprehensive HPTLC analysis of domestic HRM and herbal drugs for research, prevention of adulteration, standardization of initial HRM and its finished product, pharmaceutical development, and stability studies.

Radiolsotopes as metabolic labels for Glossina (Diptera: Glossinidae). I. Laboratory determination of the relationship between radioisotope metabolism, respiration and temperature

1978 ◽  
Vol 68 (1) ◽  
pp. 1-10 ◽  
Author(s):  
P. Taylor
Keyword(s):  
Oxygen Consumption ◽  
Related Species ◽  
Regression Line ◽  
Glossina Morsitans ◽  
Closely Related Species ◽  
Metabolic Label ◽  
Laboratory Determination ◽  
The Relationship

AbstractThe turnover of the radioisotope 137Cs is shown to be of a one-component nature and to be positively correlated with temperature for males of both Glossina morsitans Westw. and G. pallidipes Aust. A direct correlation with oxygen consumption is also demonstrated, and the relation for both species may be described by the same regression line. The feasibility of using 137Cs as a metabolic label for tsetse is therefore confirmed, and it is suggested that the predictive nature of the relationship between radioisotope turnover and metabolism may extend beyond closely related species.

Differentiation of Penicillium roqueforti from Closely Related Species Contaminating Cheeses and Dairy Environment

Fermentation ◽  
2021 ◽  
Vol 7 (4) ◽  
pp. 222
Author(s):  
Miloslava Kavková ◽  
Jaromír Cihlář ◽  
Vladimír Dráb ◽  
Ladislav Bár
Keyword(s):  
Related Species ◽  
Morphological Variability ◽  
The Other ◽  
Morphological Properties ◽  
Penicillium Roqueforti ◽  
Molecular Tools ◽  
Penicillium Species ◽  
Culture Collections ◽  
Closely Related Species

Currently, Penicillium roqueforti and the closely related P. carneum and P. paneum are identified based on their macromorphology, micromorphology, and molecular properties, the determination of which involves time-consuming procedures. Culture collections focused on dairy isolates of P. roqueforti require quick and efficient tools for routine applications to identify the (a) taxonomy affiliation and (b) morphological properties of strains that influence the sensory properties of blue-veined cheeses. Here, we assessed the morphological variability of P. roqueforti, P. carneum, P. paneum, and P.crustosum on artificial, Edam-like, and Roquefort-like media. Molecular tools were used to test P. roqueforti strains and clones effectively. A novel primer, PrsF, was tested for specificity within strains and isolates of P. roqueforti compared to P. carneum, P. paneum, and P. crustosum. The results reveal that PrsF was specific to the P. roqueforti samples and did not amplify the other tested Penicillium species. Identification based simultaneously on the specificity of the PrsF primer pair and cultivation of P. roqueforti strains on Roquefort-like medium represents an effective method for expanding the collections and practical use of P. roqueforti in the dairy industry.

Microsatellite Marker: Importance and Implications of Cross-genome Analysis for Finger Millet (Eleusine coracana (L.) Gaertn)

2020 ◽  
Vol 9 (3) ◽  
pp. 160-170
Author(s):  
Thumadath P.A. Krishna ◽  
Maharajan Theivanayagam ◽  
Gurusunathan V. Roch ◽  
Veeramuthu Duraipandiyan ◽  
Savarimuthu Ignacimuthu
Keyword(s):  
Molecular Marker ◽  
Microsatellite Markers ◽  
Ssr Markers ◽  
Genome Analysis ◽  
Related Species ◽  
Finger Millet ◽  
Crop Improvement ◽  
Human Beings ◽  
Closely Related Species ◽  
Genome Amplification

Finger millet is a superior staple food for human beings. Microsatellite or Simple Sequence Repeat (SSR) marker is a powerful tool for genetic mapping, diversity analysis and plant breeding. In finger millet, microsatellites show a higher level of polymorphism than other molecular marker systems. The identification and development of microsatellite markers are extremely expensive and time-consuming. Only less than 50% of SSR markers have been developed from microsatellite sequences for finger millet. Therefore, it is important to transfer SSR markers developed for related species/genus to finger millet. Cross-genome transferability is the easiest and cheapest method to develop SSR markers. Many comparative mapping studies using microsatellite markers clearly revealed the presence of synteny within the genomes of closely related species/ genus. Sufficient homology exists among several crop plant genomes in the sequences flanking the SSR loci. Thus, the SSR markers are beneficial to amplify the target regions in the finger millet genome. Many SSR markers were used for the analysis of cross-genome amplification in various plants such as Setaria italica, Pennisetum glaucum, Oryza sativa, Triticum aestivum, Zea mays and Hordeum vulgare. However, there is very little information available about cross-genome amplification of these markers in finger millet. The only limited report is available for the utilization of cross-genome amplified microsatellite markers in genetic analysis, gene mapping and other applications in finger millet. This review highlights the importance and implication of microsatellite markers such as genomic SSR (gSSR) and Expressed Sequence Tag (EST)-SSR in cross-genome analysis in finger millet. Nowadays, crop improvement has been one of the major priority areas of research in agriculture. The genome assisted breeding and genetic engineering plays a very crucial role in enhancing crop productivity. The rapid advance in molecular marker technology is helpful for crop improvement. Therefore, this review will be very helpful to the researchers for understanding the importance and implication of SSR markers in closely related species.

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