scholarly journals In Vitro Secondary Embryogenesis Derived from Meta-Topoline Treatment on Mass Propagation of Phalaenopsis ‘AMP 17’

2016 ◽  
Vol 8 (1) ◽  
pp. 62-68
Author(s):  
Dewi PRAMANIK ◽  
Muhammad PRAMA YUFDY ◽  
Herni SHINTIAVIRA ◽  
Budi WINARTO
Keyword(s):  
Secondary Embryogenesis ◽  
Multiplication Rate ◽  
Market Demand ◽  
Mass Propagation ◽  
Flower Stalk ◽  
High Productivity ◽  
The Third ◽  
Secondary Somatic Embryo ◽  
Half Strength

Phalaenopsis ‘AMP 17’ is an important orchid commodity in Indonesia with high market demand; however, scaling up the orchid commercially is constrained by the availability and sustainability of qualified seedlings. To overcome the problem, a reliable in vitro propagation protocol, especially via secondary embryogenesis, was undertaken. In the present study, in vitro secondary embryogenesis derived from meta-topoline (mT) treatment on mass propagation of Phalaenopsis ‘AMP 17’ was successfully established. Embryos, as explant sources, were prepared by culturing meristem tips of flower stalk shoots on Murashige and Skoog (MS) medium containing 1.5 mg/L thidiazuron (TDZ) and 0.25 mg/L N6-benzylaminopurine (BAP) for ± 3 months. High secondary somatic embryo (SSE) formation up to 64.90% with 12.30 SSEs regenerated per embryo was determined on half-strength MS augmented with 0.5 mg/L BAP and 2.5 mg/L mT. The combination also stimulated the result of high multiplication rate of SSE formation, up to 10.1 fold on the third subculture, maintained low conversion rate of germinated-embryos down to 55% and improved qualified-growth of the germinated embryos. The mT treatment produced 86% survival plantlets with high qualified-performance. The system could be applied as an alternative method to step forward towards an improved propagation protocol, commercially efficient due to high productivity. Detail findings in each step were discussed.

scholarly journals In vitro Embryogenesis Derived from Shoot Tips in Mass Propagation of Two Selected-Clones of Phalaenopsis

2016 ◽  
Vol 8 (3) ◽  
pp. 317-325 ◽  
Author(s):  
Budi WINARTO ◽  
Kristina Dwi ATMINI ◽  
Dedeh Siti BADRIAH ◽  
Mega WEGADARA
Keyword(s):  
Economic Value ◽  
Shoot Tips ◽  
Ms Medium ◽  
Market Demand ◽  
Mass Propagation ◽  
Full Strength ◽  
Half Strength ◽  
Alternative Choice ◽  
In Vitro Embryogenesis

Phalaenopsis is of high economic value and market demand in Indonesia; however, orchid products are mostly imported from other countries. ‘Kristina Dwi’ (KD) 69.274 and ‘Dedeh’ (D) 802.28 are two selected clones with high potential utilized and developed commercially. To support their commercialization, a reliable in vitro propagation protocol is essential.  In the current study, an in vitro mass propagation protocol for KD 69.274 and D 802.28 clones was successfully established using shoot tips as explant sources. A high number of embryos, up to 8.2 embryos per explant, with 58.5% explant regeneration, and 3.5 regenerated-explants in average were regenerated from shoot tips of KD 69.274 clone cultured on  half-strength Murashige and Skoog (MS) medium, with full strength micro, Fe-chellate and vitamin containing 0.5 mg/L thidiazuruon (TDZ) and 0.25 mg/L N6-benzyladenine (BA). The initial embryos were proliferated by culturing embryos individually on half-strength MS medium with 0.13 mg/L TDZ and 0.25 mg/L BA and resulted in high embryo regeneration up to 91.4%, with 10.2 embryos per explant and no embryo browning. The embryos were multiplied under periodical subcultures of 3 months each, resulting in gradual increasing number of embryos from the first subculture till the fifth subculture, with 23.6 embryos produced, then declined afterward. The embryos were easily germinated on half-strength MS medium with full strength of vitamin and hormone free, with 73.9% embryo germination and 14.9 germinated embryos. Healthy plantlets were stimulated on the same medium with 2 g/L activated charcoal (AC) and successfully acclimatized on Cycas rumphii bulk, with 88.3% survival plantlets. Finally, it can be summarized that a new in vitro mass propagation protocol, as new alternative choice for Phalaenopsis propagation, was successfully established.

scholarly journals Micropropagation of Mini Orchid Hybrid Phalaenopsis “Sogo Vivien”

2016 ◽  
Vol 1 (1) ◽  
pp. 45 ◽  
Author(s):  
Exsyupransia Mursyanti ◽  
Aziz Purwantoro ◽  
Sukarti Moeljopawiro ◽  
Endang Semiarti
Keyword(s):  
Cell Shape ◽  
Histological Analysis ◽  
The Other ◽  
Mass Propagation ◽  
Flower Stalk ◽  
Shoot Segment ◽  
Plant Body ◽  
Pot Plant ◽  
Variegated Plant

Phalaenopsis “Sogo Vivien” is an orchid hybrid with mini size plant body, and exhibits numerous beautiful pink flowers, that is ideal as ornamental pot plant. Some plants of this orchid exhibit variegated leaves that improve the beauty of the plant, not only because of the flower but also as attracted leaves. This orchid has high economical value, but mass propagation of this orchid has not established yet. An effective method to propagate both the normal and variegated plants is worth to be generated. The objective of this research was to produce a large number of P. “Sogo Vivien” plants, including the variegated plants. The method used seeds from self pollinating variegated plant, and flower stalk nodes. The seeds were sown on three various medium: VW, NP and MS, and flower stalk nodes were planted on VW + BA 10 mg l-1 + active carbon. The results showed that the best medium for in vitro culture of P. “Sogo Vivien” was NP medium, in which all seeds could grew into plantlets. Most plantlets emerged from the seeds were non variegated, only one plantlet out of 1344 seeds was variegated (0.007%). Although all emerged plantlets from flower stalk exhibited variegated leaves. Particularly, the plantlets arised from the second and third basal nodes of flower stalk showed the highest growth rate than that from the other nodes. Histological analysis showed that at 11-13 days after shoot segment plantation on NP medium, the shape of apical cells in the nodes was changed, then followed by the change of cell shape in the basal part of the nodes, produced bipolar pattern, then gradually developed into shoot. These results suggest that mass propagation could be achieved using seed culture, but to get the variegated phenotypes, the second and third nodes of flower stalk from variegated plant were the best explants to be used.

scholarly journals In Vitro Propagation of Three Moroccan Prickly Pear Cactus Opuntia and Plant Establishment in Soil

2013 ◽  
Vol 5 (1) ◽  
pp. 39-44 ◽  
Author(s):  
Aissam EL FINTI ◽  
Rachida EL BOULLANI ◽  
Naima AIT AABD ◽  
Fouad MSANDA ◽  
Mohammed A. SERGHINI ◽  
...  
Keyword(s):  
Basal Medium ◽  
Prickly Pear ◽  
Multiplication Rate ◽  
In Vitro Micropropagation ◽  
Prickly Pear Cactus ◽  
Half Strength ◽  
Micropropagated Plants ◽  
Large Production ◽  
Pear Cactus

Opuntia is one of the most widespread cacti, primarily due to their edible fruit and vegetable mass used as feed. The high demand for young plants of Opuntia made it necessary to find a rapid method of multiplication of the cactus, the safest method consisting in vitro micropropagation of species belonging to this genus. With aim of large production of plant material, a propagation system of three important prickly pear cactus cultivar (Opuntia ficus-indica) in Morocco was developed. Segments of healthy young cladode (containing one areole) were cultivated in Murashige and Skoog medium (MS) containing adenine sulfate (40 mg/1), monosodium phosphate (50 mg/l), sucrose (50 g/l), phytagel (0.3%) and benzyladenine (BA) at 22.2 μM, to start the process of micropropagation. In vitro-developed shoots from areoles were used as secondary explants to induce shoot development in the MS medium with 5 mg/l of BA. All of the three studied cultivars showed an important multiplication rate in this medium. ‘Sidi Ifni M’ (‘Moussa’) cultivar shows the greatest number of shoots followed by ‘Sidi Ifni A’ (‘Aissa’) and ‘Delahia’ 17.26, 14.12 and 12.13 respectively. Rooting of in vitro-generated shoots was achieved most efficiently on half-strength MS basal medium supplemented with 0.5 mg/l of indole-3-butyric acid (IBA) or IAA. Rooting frequencies were in the range from 95 to 100% and the highest mean number of root (19.1) was obtained with IBA for ‘Delahia’ cultivar. All micropropagated plants were transferred to greenhouse and all of them survived acclimatization process and showed good overall growth.

scholarly journals In Vitro Long-Term Cultures of Papaya (Carica Papaya L.).

2021 ◽  
Author(s):  
Jose Javier Regalado González ◽  
Manuel López Granero ◽  
Carlos Lopez Encina
Keyword(s):  
Morphological Characteristics ◽  
Basal Medium ◽  
Multiplication Rate ◽  
Ms Medium ◽  
High Quality ◽  
Poor Growth ◽  
Average Multiplication ◽  
Half Strength

Abstract We present the data on proliferation corresponding to 10 years of continuous incubation in vitro of papaya shoots, and propose a reliable method for long-term micropropagation for papaya, using two types of explants: Microshoots from somatic embryos, and from axillary buds of papaya. Three different media were assayed. The proliferation medium (PPRM) allowed to maintain papaya shoots under continuous proliferation during 20 years, maintaining a consistent behaviour. Most of the shoots developed in PPRM rooted during the incubation, and after acclimated easily, maintaining the morphological characteristics of the parental plants, flowering and setting fruits normally. The PPRM medium consist in MS medium supplemented with NAA (0.1 mg l-1), BA (0.5 mg l-1), GA3 (0.5 mg l-1) and Adenine sulphate (40 mg l-1). The average multiplication rate was higher than 20 shoots per explant along the long-term assay. The elongation medium (PELM), was designed to recover shoots with a poor growth, and allowed the development of high quality shoots ready for rooting, and consist in a MS basal medium supplemented with NAA (0.1 mg l-1), Kin (0.5 mg l-1) and GA3 (1 mg l-1). The rooting medium (PROM) was designed to induce high quality roots from non-rooted shoots and consist in a half strength MS medium plus IBA (1mg l-1). On PROM, agar can be exchanged for expanded vermiculite. Acclimation took place inside an acclimatization tunnel under progressive hydric stress. After 4 weeks, the plant recovery rate was 90% for plants maintained under continuous proliferation during ten years.

scholarly journals Dual Phase Regeneration System for Mass Propagation of Cymbidium aloifolium (L.) Sw.: A High Value Medicinal Orchid

2019 ◽  
Vol 29 (2) ◽  
pp. 257-266
Author(s):  
Mohammad Musharof Hossain ◽  
Madhu Sharma
Keyword(s):  
Root Systems ◽  
Dual Phase ◽  
Mass Propagation ◽  
Regeneration System ◽  
Prolonged Culture ◽  
Half Strength ◽  
Cymbidium Aloifolium ◽  
Medicinal Orchid ◽  
Stem Segments

Efficient micropropagation protocol was established in Cymbidium aloifolium through induction of secondary (2°) protocorms from primary (1°) protocorms, shoot buds (SBs) and protocorm-like bodies (PLBs) from pseudo-stem segments of in vitro raised seedlings. Neo-formation of 2° protocorms from 1° protocorms was obtained in liquid and agar-solidified Phytamax (PM) medium with NAA, 2,4-D, BAP and Kn at different concentrations and combinations. The highest number of 2° protocorms was induced in liquid PM medium (9.76 ± 0.88/1° protocorm) amended with 2.0 mg/l BAP + 1.0 mg/l 2,4- D. Although protocorms proliferated profusely in liquid medium, hyperhydricity was observed after prolonged culture. Both SBs and PLBs were induced from pseudo-stem segments on agar gelled PM medium and the mode of differentiation was dependent upon specific PGRs concentrations. The maximum number of SBs (5.80 ± 2.59/explant) was recorded in BAP and NAA at 1.0 mg/l each, while the maximum number of PLBs (7.20 ± 3.22/explant) were induced in 2.0 mg/l BAP and NAA each. Well developed root systems were induced SBs and PLBs on transferring to half-strength PM medium fortified with 0.5 mg/l IAA. The rooted plantlets were transferred to the greenhouse with 95% survival.

scholarly journals In vitro Mass Clonal Propagation of Spathoglottis plicata Blume

1970 ◽  
Vol 19 (2) ◽  
pp. 151-160 ◽  
Author(s):  
Pinaki Sinha ◽  
M. Lokman Hakim ◽  
M. Firoz Alam
Keyword(s):  
Clonal Propagation ◽  
Nodal Segments ◽  
Fluorescent Light ◽  
Plantlet Formation ◽  
The Third ◽  
Regenerated Plants ◽  
Rooting Medium ◽  
Half Strength ◽  
In Vitro Clonal Propagation

For in vitro clonal propagation of Spathoglottis plicata Blume nodal segments of young shoots were cultured on half strength of MS  with  2% sucrose + 2.0 mg/l BA + 0.5 mg/l NAA + 2 g/l peptone + 15% (v/v) CW + 0.5 g/l AC,  incubated at 24 ± 2ºC under 3000 lux fluorescent light for a 16 hr photoperiod per day. About 19 micro-shoots were induced from the explants within 12 weeks. Subculture of micro-shoots for eight weeks on the same nutrient medium enhanced the number of micro-shoots up to 60. The clumps of the micro-shoots were dissected and cultured on half strength of MS  with 2% sucrose + 2 g/l peptone + 15% (v/v) CW + 0.5 g/l AC + 200 mg/l L-glutamine. The micro-shoot sections elongated to form shoots, and new micro shoots were induced from the base within eight weeks of culture. For plantlet formation the best rooting medium was determined as  half strength of MS  with 2% sucrose + 2 g/l peptone + 15% (v/v) CW + 0.5 g/l AC + 50 g/l banana pulp. After rearing 25 g mixture of urea, TSP and MOP (2 : 1 : 1) were applied per plant at three months intervals. All the regenerated plants blossomed on the third year. Key words: Spathoglottis plicata, Clonal propagation, Acclimation D.O.I. 10.3329/ptcb.v19i2.5432 Plant Tissue Cult. & Biotech. 19(2): 151-160, 2009 (December)

scholarly journals Micropropagation of Eclipta alba (Linn.) Hassk- a Valuable Medicinal Herb

1970 ◽  
Vol 43 (2) ◽  
pp. 215-222 ◽  
Author(s):  
AKM Sayeed Hassan ◽  
Farhana Afroz ◽  
Laila Shamroze Bari ◽  
John Liton Munshi ◽  
Miskat Ara Akhter Jahan ◽  
...  
Keyword(s):  
Room Temperature ◽  
Basal Medium ◽  
Shoot Proliferation ◽  
Shoot Multiplication ◽  
Medicinal Herb ◽  
Mass Propagation ◽  
Eclipta Alba ◽  
Half Strength ◽  
Regenerated Plantlets

A protocol was established for mass propagation of a valuable medicinal herb, Eclipta alba (Linn.) Hassk (Asteraceae) through in vitro culture. Apical and axillary buds of young sprouts from selected plants were used as explants. Best shoot induction was observed on MS basal medium supplemented with 0.5 mgl-1 BAP + 0.1 mgl-1 NAA, in which 94% of the explants produced 18 shoots per culture. Repeated subcultures in the same medium, resulted rapid shoot multiplication with 26 shoots per culture. In vitro raised shoots rooted on half strength MS medium with 1.0 mgl-1 IBA +1.0 mgl-1 NAA. For acclimatization and transplantation, the plantlets in the rooting culture tubes were kept in normal room temperature for 7 days before transplanting in pots where plantlets were reared for three weeks. The survival rate of regenerated plantlets was 80%. Key words: Eclipta alba, Medicinal plant, Shoot proliferation, Micropropagation, Acclimatization   DOI: 10.3329/bjsir.v43i2.965 Bangladesh J. Sci. Ind. Res. 43(2), 215-222, 2008 

scholarly journals In vitro Direct Regeneration from Node and Leaf Explants of Phalaenopsis cv. ‘Surabaya’

2016 ◽  
Vol 25 (2) ◽  
pp. 193-205 ◽  
Author(s):  
Khosro Balilashaki ◽  
Maryam Vahedi ◽  
Roghayeh Karimi
Keyword(s):  
Shoot Regeneration ◽  
Leaf Explants ◽  
Nodal Explants ◽  
Direct Regeneration ◽  
Flower Stalk ◽  
Breeding Programs ◽  
Half Strength ◽  
Direct Embryogenesis ◽  
Regenerated Plantlets

An efficient and reproducible procedure for the direct regeneration of phalaenopsis cv. ‘Surabaya’ using of nodal explants and leaf segments derived from in vitro flower stalk was conducted. Three experiments were carried out for shoot development and subsequent plant regeneration: Direct shoot regeneration from nodal explants of Phalaenopsis cv. ‘Surabaya’ flower stalks on MS added with different combination of NAA and BAP, direct regeneration of protocormlike bodies (PLBs) from leaf explants in a MS with different concentrations of the TDZ, acclimatization of regenerated plantlets in different mixture of components and nutrients. The results showed that 5 mg/l BAP and 2 mg/l NAA were most effective concentration for shoot regeneration, regenerated shoots were cultured on half strength of MS containing activated charcoal, IAA and NAA at various concentrations, highest number of root (6.7) was obtained in higher concentration of NAA (2 mg/l). TDZ induced a higher frequency of embryogenesis from leaf explants than BAP, the highest number of embryos per explant was 22.45 at 3 mg/l TDZ. Altogether, BAP at higher concentration (10 mg/l) with 1 mg/l NAA had the highest enhancement on the amount of direct embryogenesis. In our investigation 87.20% plantlets via nodal explants survived acclimatization process in medium containing cocopeat and coal (1 : 1). The survival rate of regenerated plantlets via nodal explants (82.07%) was more than of regenerated plantlets via leaf explants (70.47). This protocol provides the basis for further investigation on micropropagation and breeding programs in Phalaenopsis cv. ‘Surabaya’.Plant Tissue Cult. & Biotech. 25(2): 193-205, 2015 (December)

Bulgarian golden root in vitro cultures for micropropagation and reintroduction

2010 ◽  
Vol 5 (6) ◽  
pp. 853-863 ◽  
Author(s):  
Krasimira Tasheva ◽  
Georgina Kosturkova
Keyword(s):  
Germplasm Conservation ◽  
Shoot Formation ◽  
Alternative Methods ◽  
Wild Plants ◽  
Multiplication Rate ◽  
In Vitro Techniques ◽  
Seedling Explants ◽  
Efficient Scheme ◽  
Half Strength

AbstractRhodiola rosea is an endangered medicinal plant used for cancer, cardiovascular, and nervous system diseases therapy. Due to its limited distribution and sustainability alternative methods for production of its valuable substances are under investigation. Using in vitro techniques apical and rhizome buds, leaf nodes, stem and radix segments from wild plants and in vitro seedlings were plated on 24 modified Murashige and Skoog (1962) media. Decontamination of plant material was successful only in 21% of the schemes. The best shoot induction was obtained from seedling explants on media containing 2 mg/l zeatin or N6-benzylaminopurine, each. Their reduction stimulated shoot formation in the next passages (multiplication rate up to 5). Efficient rooting was induced on half-strength MS with 2 mg/l Indole-3-butyric acid and stimulated by adding 0.2 mg/l Indolyl-3-acetic acid. Regenerants rooted in perlite, peat, and soil (1:1:2), adapted in greenhouse, and transplanted in the mountains survived (70%) and developed like the wild plants. Salidroside content of these plants after one or two years was high (0.64 and 0.61% in rhizomes and 0.62 and 0.53% in roots, respectively). This is the first established efficient scheme for micropropagation of Bulgarian R. rosea allowing habitats restoration, germplasm conservation, and potential application of biotechnology for production of valuable substances.

scholarly journals Elicitation of Phenylpropanoids in Maqui (Aristotelia chilensis [Mol.] Stuntz) Plants Micropropagated in Photomixotrophic Temporary Immersion Bioreactors (TIBs).

2021 ◽  
Author(s):  
Giulia E Trentini ◽  
Makarena Rojas ◽  
Daniela Gajardo ◽  
Débora Alburquenque ◽  
Evelyn Villagra ◽  
...  
Keyword(s):  
Culture Medium ◽  
Wild Plants ◽  
Control Treatment ◽  
Multiplication Rate ◽  
Fresh Weight ◽  
Temporary Immersion ◽  
Air Treatment ◽  
The Third ◽  
Aristotelia Chilensis

Abstract A biotechnological system for the production of plants biomass and phenylpropanoids of maqui was developed in photomixotrophic TIBs. The in vitro maqui multiplication was evaluated using combinations of TDZ and BAP in TIBs 1L capacity. Treatment of MS basal supplemented with TDZ 1 mg/l shows the best results for the variables fresh weight and multiplication rate. Photomixotrophic conditions were standardized in media with 3%, 2%, 1%, 0% sucrose at a light intensity of 100 µM m− 2s− 1. The treatments reduced in sucrose (1% and 2%) and air supplemented with 0.4 MPa CO2 do not differ significantly in biomass production (fresh weight per cluster of plants) compared to the control treatment with sucrose 3% and standard air. Treatment with ABA (1m/l) induced the production and accumulation of phenylpropanoids metabolites in maqui cultures bioreactors. Phenylpropanoids in in vitro biomass of maqui and culture medium from TIBs were determined in parallel with control samples from wild plants and mature fruits. After the third day of analysis, not significant differences in polyphenols and anthocyanin contents were verified between the treatments of maqui in TIBs + ABA and controls. The non-significant differences in the contents of polyphenols and anthocyanin were maintained until the 15 days of analysis. The antioxidant capacity comparing samples of maqui in bioreactors and wild plants showed no significant differences by the ORAC test from day 5 to day 15 of the study.

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