scholarly journals Phytochemical analysis and antioxidant capacity ofTabernaemontana catharinensis A. DC. Fruits and branches

2014 ◽  
Vol 86 (2) ◽  
pp. 881-888 ◽  
Author(s):  
MARIANA PIANA ◽  
ALINE A. BOLIGON ◽  
THIELE F. DE BRUM ◽  
MARINA ZADRA ◽  
BIANCA V. BELKE ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Ethyl Acetate ◽  
Antioxidant Capacity ◽  
Chlorogenic Acid ◽  
Condensed Tannins ◽  
High Performance ◽  
Ethyl Acetate Fraction ◽  
Phytochemical Analysis ◽  
Dpph Method

The antioxidant capacity of the crude extract and fractions ofTabernaemontana catharinensis fruits and branches, was evaluated by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method and the content of polyphenols, flavonoids, alkaloids and condensed tannins were determined by the spectrophotometric method. The ethyl acetate fraction of the fruits and the n-butanol fraction of the branches showed IC50 of 181.82 µg/mL and 78.19 µg/mL, respectively. All fractions were analyzed by high performance liquid chromatography (HPLC), in the branches were quantified chlorogenic acid in the chloroform (8.96 mg/g), ethyl acetate (4.31 mg/g) and n-butanol (3.33 mg/g) fractions; caffeic acid in the ethyl acetate (5.24 mg/g) and n-butanol (1.81 mg/g); gallic acid (0.52 mg/g) in the n-butanol. In the fruits, chlorogenic acid in the chloroform (1.67 mg/g); rutin in the ethyl acetate (3.45 mg/g) and n-butanol (8.98 mg/g) fractions. The present study showed that these quantified compounds can contribute to antioxidant capacity which was higher in the branches than in the fruits.

Development and Validation of a High-Performance Liquid Chromatography Method for Standardization of the Bioactive Ethyl Acetate Fraction of Alstonia scholaris (Linn.) R. Br. Growing in Egypt

2013 ◽  
Vol 68 (9-10) ◽  
pp. 376-383
Author(s):  
Hesham I. El-Askary ◽  
Mahmoud M. El-Olemy ◽  
Maha M. Salama ◽  
Mahetab H. Amer
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Ethyl Acetate ◽  
High Performance ◽  
Ethanolic Extract ◽  
Ethyl Acetate Fraction ◽  
Hplc Method ◽  
Spectroscopic Techniques ◽  
Alstonia Scholaris ◽  
Relative Standard

Bio-guided fractionation of the ethanolic extract of the leaves of Alstonia scholaris (Apocynaceae) growing in Egypt was carried out to evaluate its antihyperglycemic acti vity in alloxan-induced diabetic rats and its hepatoprotective activity against CCl4-induced hepatotoxicity in rats. The ethyl acetate fraction of the ethanolic extract showed the highest antihyperglycemic [(133.6 ± 4.2) mg/mL, relative to metformin with (92.3 ± 2.7) mg/mL] and hepatoprotective [(37.9 ± 1.4) U/L, relative to silymarin with (29.7 ± 0.8) U/L] activities. Four compounds were isolated from this fraction, and identifi ed by spectroscopic techniques and by comparison with reported data: caffeic acid and isoquercitrin for the fi rst time from this plant, in addition to quercetin 3-O-β-D-xylopyranosyl (1''' →2")-β-D-galactopyranoside (major compound) and chlorogenic acid. A validated reversed phase-high-performance liquid chromatography (RP-HPLC) method was developed for the standardization of the bio active ethyl acetate fraction. The calibration curve showed good linearity (r2 > 0.999) within tested ranges. The relative standard deviation of the method was less than 3% for intra- (0.4 - 2.0%) and inter-day (1.9 - 2.8%) assays. Mean recovery of the method was within the range of 98.5 - 102.5%. The minimum detectable concentration of the analyte (LOD) was found to be 0.04 μg/mL. This developed HPLC method was shown to be simple, rapid, precise, reproducible, robust, specifi c, and accurate for quality assessment of the bioactive fraction

scholarly journals Schisandra rubriflora Plant Material and In Vitro Microshoot Cultures as Rich Sources of Natural Phenolic Antioxidants

Antioxidants ◽  
2020 ◽  
Vol 9 (6) ◽  
pp. 488
Author(s):  
Agnieszka Szopa ◽  
Michał Dziurka ◽  
Sebastian Granica ◽  
Marta Klimek-Szczykutowicz ◽  
Paweł Kubica ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Phenolic Compounds ◽  
Antioxidant Capacity ◽  
Phenolic Acids ◽  
High Performance ◽  
Total Phenolic ◽  
Array Detector ◽  
Plant Materials

Schisandra rubriflora is a dioecious, underestimated medicinal plant species known from traditional Chinese medicine. The present study was aimed at characterising the polyphenolic profile composition and the related antioxidant capacity of S. rubriflora fruit, stem and leaf and in vitro microshoot culture extracts. Separate analyses of material from female and male specimens were carried out. This study was specifically aimed at detailed characterisation of the contribution of phenolic compounds to overall antioxidant activity using ultra-high-performance liquid chromatography with a photodiode array detector coupled to electrospray ionization ion trap mass spectrometry (UHPLC-DAD-ESI-MS3) and a high-performance liquid chromatography-diode array detector (HPLC-DAD). Using UHPLC-DAD-ESI-MS3, twenty-seven phenolic compounds from among phenolic acids and flavonoids were identified. Concentrations of three phenolic acids (neochlorogenic, chlorogenic and cryptochlorogenic acids) and eight flavonoids (hyperoside, rutoside, isoquercitrin, guaijaverin, trifolin, quercetin, kaempferol, and isorhamnetin) were determined using HPLC-DAD using reference standards. The highest total phenolic content was confirmed for the stem and leaf extracts collected in spring. The contents of phenolic compounds of in vitro biomasses were comparable to that in the fruit extracts. The methanolic extracts from the studied plant materials were evaluated for their antioxidant properties using various in vitro assays, namely free radicals scavenging estimation using 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH), ferric-reducing antioxidant power (FRAP) and cupric-reducing antioxidant capacity (CUPRAC) as well as QUick, Easy, New, CHEap, and Reproducible CUPRAC (QUENCHER-CUPRAC) assays. A close relationship between the content of polyphenolic compounds in S. rubriflora and their antioxidant potential has been documented.

scholarly journals Procyanidins in Lotus L. genotypes grown in soil with different saturations of aluminum

2016 ◽  
Vol 37 (1) ◽  
pp. 85
Author(s):  
Sílvia Ortiz Chini ◽  
Pedro Alexandre Varella Escosteguy ◽  
Simone Meredith Scheffer-Basso ◽  
Andréa Michel Sobottka ◽  
Charise Dallazem Bertol ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Environmental Conditions ◽  
Condensed Tannins ◽  
High Performance ◽  
Soil Acidity ◽  
Opposite Effect ◽  
Similar Proportion ◽  
Environment Interaction ◽  
Genotype Environment Interaction

Condensed tannins are formed by monomers of procyanidins and prodelfinidins, where the proportion and concentration of their monomers varies according to the plant species and environmental conditions. In Lotus spp., condensed tannins prevent tympanism in ruminants that feed on them. This study aimed to evaluate the concentration of procyanidins and their monomers, catechin and epicatechin in the genotypes of Lotus L. grown in soil with different saturations of aluminum. A two-factor (genotype × Al saturation) assay was performed, where the genotypes São Gabriel, Ganador, and UFRGS (Lotus corniculatus L.); Serrano (Lotus uliginosus); and El Rincón (Lotus subbiflorus) were cultivated in soil with an Al saturation of 0-20%. The procyanidins were evaluated using high-performance liquid chromatography, which was previously validated for catechin and epicatechin. The concentration of procyanidins and the proportion of epicatechin:catechin were affected by the genotype × environment interaction. In L. corniculatus and L. subbiflorus, the concentration of procyanidin was significantly higher when they were grown in the soil with an Al saturation of 20% compared to that when they were grown in the soil with 0% Al saturation, but the opposite effect was observed in L. uliginosus. The proportion of epicatechin:catechin decreased in plants grown in soil without Al, and only the UFRGS genotype maintained a similar proportion under both the soil acidity conditions. The predominant monomer was epicatechin, which varied from 57% to 75% according to the soil in which the plants were grown.

Optimization of Preparation Technology on Medicated Serum of Yinqiaotiangan in Mice

2013 ◽  
Vol 634-638 ◽  
pp. 1328-1331
Author(s):  
Hong Jun Wang ◽  
Tie Zhong Zhou ◽  
Hong Jiang ◽  
Zun Lai Sheng
Keyword(s):  
Response Surface Methodology ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Chlorogenic Acid ◽  
High Performance ◽  
Sampling Time ◽  
Blood Sampling ◽  
Single Factor ◽  
Preparation Technology ◽  
Influent Factors

Response surface methodology (RSM) and single factor test were used to optimize preparation technology on medicated serum of Yinqiaotiangan (YQTG) in mice. The influent factors of preparing medicated serum include blood sampling time, administration times, and administration dosage. The content of chlorogenic acid in medicated serum was detected by high performance liquid chromatography (HPLC). Results showed that the optimum preparation technology of medicated serum is 33 minutes of blood sampling time, 4 times of administration times and 0.024g/mL of administration concentration. Under this condition, the concentration of chlorogenic acid reached peak 33.34 μg/mL in mice medicated serum. It is seen that the RSM method effectively and accurately optimize preparation technology on medicated serum of YQTG preparation.

Chromatographic behaviour of some porphyrins and their complexes with Zn, Pd(II) and Cu(II)

2000 ◽  
Vol 04 (02) ◽  
pp. 209-214 ◽  
Author(s):  
M. I. UVAROVA ◽  
G. D. BRYKINA ◽  
O. A. SHPIGUN
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Ethyl Acetate ◽  
Mobile Phase ◽  
High Performance ◽  
Reversed Phase ◽  
Zinc Complexes ◽  
Acetate Concentration ◽  
Hydrophobic Compounds ◽  
Retention Parameters

In this work the influence of the porphyrin structure and of the nature of the mobile phase upon retention parameters is examined by means of reversed phase high-performance liquid chromatography (HPLC). Acetonitrile–ethyl acetate and other mixtures were used as eluents. An increase in the retention of azo- and benzosubstituted porphyrins as well as of those containing a large number of carbon atoms as substituents of macrocycles may be noted. A variation in the polarity of the mobile phase affects the retention of the ligands more than that of their zinc complexes. The retention of the most hydrophobic compounds may be well described in coordinates lg k'– lg M. For less hydrophobic porphyrins these dependences are close to linear only within limited intervals of mobile phase ethyl acetate concentration. The best separation of zinc complexes was achieved with acetonitrile as the eluent. The detection limit of porphyrin ligands and complexes with metals is n × 10-8 M.

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