scholarly journals Impact of phosphate solubilizing bacteria on wheat (Triticum aestivum ) in the presence of pesticides

2019 ◽  
Vol 79 (1) ◽  
pp. 29-37 ◽  
Author(s):  
I. Munir ◽  
A. Bano ◽  
M. Faisal
Keyword(s):  
Growth Parameters ◽  
Phosphate Solubilizing Bacteria ◽  
Acidic Ph ◽  
Pseudomonas Sp ◽  
Percentage Germination ◽  
Pesticide Stress ◽  
Auxin Production ◽  
Rrna Sequencing ◽  
Biochemical Testing ◽  
Phosphate Solubilizing

Abstract Three phosphate solubilizing bacteria were isolated and identified by 16S rRNA sequencing as Pseudomonas putida, Pseudomonas sp and Pseudomonas fulva . The strains were subjected to plant biochemical testing and all the PGPR attributes were checked in the presence of pesticides (chlorpyrifos and pyriproxyfen). The phosphate solubilizing index of strain Ros2 was highest in NBRIP medium i.e 2.23 mm. All the strains showed acidic pH (ranges from 2.5-5) on both medium i.e PVK and NBRIP. Strain Ros2 was highly positive for ammonia production as well as siderophore production while strain Rad2 was positive for HCN production. The results obtained by the strains Rad1, Rad2 and Ros2 for auxin production were 33.1, 30.67 and 15.38 µg ml-1, respectively. Strain Rad1 showed 16% increase in percentage germination in comparison to control in the presence of pesticide stress. Most promising results for chlorophyll content estimation were obtained in the presence of carotenoids upto 6 mgg-1 without stress by both strains Rad1 and Rad2. Study suggests that especially strain Ros2 can enhance plant growth parameters in the pesticide stress.

scholarly journals Rhizospheric Phosphate Solubilizing Bacillus atrophaeus GQJK17 S8 Increases Quinoa Seedling, Withstands Heavy Metals, and Mitigates Salt Stress

2021 ◽  
Vol 13 (6) ◽  
pp. 3307
Author(s):  
Ismail Mahdi ◽  
Nidal Fahsi ◽  
Mohamed Hafidi ◽  
Saad Benjelloun ◽  
Abdelmounaaim Allaoui ◽  
...  
Keyword(s):  
Contaminated Soils ◽  
Germination Rate ◽  
Chenopodium Quinoa ◽  
Hydrocyanic Acid ◽  
Phosphate Solubilizing Bacteria ◽  
Pseudomonas Sp ◽  
Bacillus Atrophaeus ◽  
Genotypic Analysis ◽  
Phosphate Solubilizing ◽  
Enterobacter Asburiae

Introduction of quinoa (Chenopodium quinoa willd.), a gluten-free nutritious pseudo-cereal, outside its traditional growing areas exposed it to seedling damping-off. Here, we isolated eleven phosphate-solubilizing bacteria from the quinoa rhizosphere and assessed their effect on germination and seedlings growth. All isolates solubilized phosphate, produced indole3-acetic acid, hydrocyanic acid, siderophores, and ammonia. Genotypic analysis revealed that our strains are related to the genus of Bacillus, Pseudomonas, and Enterobacter. Strains Enterobacter asburiae (QD14, QE4, QE6, and QE16), Enterobacter sp. QE3, and Enterobacter hormaechei QE7 withstood 1.5 mg·L−1 of cadmium sulfate, 0.5 mg·mL−1 of nickel nitrate, and 1 mg·mL−1 of copper sulfate. Moreover, all strains solubilized zinc from ZnO; P. Stutzeri QD1 and E. asburiae QD14 did not solubilize Zn3(PO4)2 and CO3Zn, whereas CO3Zn was not solubilized by E. asburiae QE16. Bacillus atrophaeus S8 tolerated 11% NaCl. P. frederiksbergensis S6 and Pseudomonas sp. S7 induced biofilm formation. Anti-fusarium activity was demonstrated for E.asburiae QE16, P. stutzeri QD1, P. frederiksbergensis S6, Pseudomonas sp. S7, and B. atrophaeus S8. Lastly, inoculation of quinoa seeds with B. atrophaeus S8 and E. asburiae QB1 induced the best germination rate and seedling growth, suggesting their potential use as inoculants for salty and heavy metal or zinc contaminated soils.

scholarly journals Phosphate Solubilizing Bacteria in Certain Agricultural Crop Soils of Delhi

2018 ◽  
Vol 4 (01) ◽  
pp. 70-75
Author(s):  
Ratul Baishya ◽  
Rhituporna Saikia
Keyword(s):  
Organic Acids ◽  
Solid Medium ◽  
Crop Yields ◽  
Crop Plants ◽  
Agricultural Crop ◽  
Phosphate Solubilizing Bacteria ◽  
Pseudomonas Sp ◽  
Phosphate Solubilizing ◽  
Phosphate Solubilizer ◽  
Halo Zone

Phosphate solubilizing bacteria (PSB) helps in the solubilization of insoluble phosphates and thus lead to increase in crop yields. A study was conducted to isolate and characterize biochemically PSB from different agricultural crop soils of Delhi such as Garlic, Radish, Chilli, Onion and Cabbage. PSB were isolated in Pikovskaya solid medium and formation of solubilization (halo) zone was measured. 16 PSB were isolated and identified. The selected PSB differed in phosphate solubilizing efficiency, production of organic acids and phosphatases. Citrobacter sp. and Pseudomonas sp. were dominant in all the crop plants. Among all the isolates, Pseudomonas sp. proved to be an efficient phosphate solubilizer.

scholarly journals Efektivitas dolomit teraktivasi yang diperkaya dengan bakteri pelarut fosfat sebagai pengganti kiserit pada bibit kakao The effectiveness of activated dolomite enriched by phosphate solubilizing bacteria as kieserite substitute on cocoa seedling

2016 ◽  
Vol 80 (1) ◽  
Author(s):  
Laksmita Prima SANTI ◽  
Didiek Hadjar GOENADI
Keyword(s):  
Acid Soils ◽  
Dry Mass ◽  
Test Plant ◽  
Phosphate Solubilizing Bacteria ◽  
Pseudomonas Sp ◽  
Npk Fertilizers ◽  
Standard Dosage ◽  
Tropical Regions ◽  
Mg Deficiency ◽  
Phosphate Solubilizing

Abstract Magnesium (Mg) deficiency is commonly found in acid soils with high weathering intensity as widely present in tropical and sub-tropical regions, or those developed from low Mg-content parent materials. To overcome Mg deficiency, dolomite is often used to replace kieserite although the former is less reactive than the later. An activation technology by heating up to 800oC, followed by sulfic acid addition, then enriched by phosphate solubilizing bacteria is prospective to improve Mg solubility in soils. This research was aimed to enhance dolomite reactivity and to determine the effectiveness of activated dolomite enriched by phosphate solubilizing bacteria as Mg nutrient sources substituting kieserite on cocoa seedling growth. The results indicate that total and solubility in water of MgO from the prototype of activated dolomite were 26.7 and 9.2%, respectively. Using cocoa seedling as test plant, the use of activated dolomite enriched by using 109 cfu of Pseudomonas sp. at 3.85 g/seedling and combined with 100% dosages of NPK fertilizers showed the best vegetative growth and total dry mass of seedlings was significantly higher than that of 100% NPK-Mg kieserite standard dosage Abstrak Defisiensi Mg umumnya ditemukan pada tanah masam dengan tingkat pelapukan tinggi yang banyak terdapat di daerah tropik, subtropik, dan tanah yang terbentuk dari bahan induk miskin Mg. Untuk mengatasi defisiensi Mg, dolomit sering digunakan sebagai pengganti kiserit walaupun sifatnya kurang reaktif jika dibandingkan kiserit. Teknologi aktivasi dengan pemanasan mencapai 800oC, dilanjutkan dengan penambahan asam sulfat serta pengkayaan dengan bakteri pelarut fosfat diharapkan dapat meningkatkan kelarutan Mg di dalam tanah. Penelitian ini bertujuan untuk meningkatkan reaktivitas dolomit serta menetapkan efektivitas dolomit teraktivasi yang diperkaya dengan bakteri pelarut fosfat sebagai sumber hara Mg pengganti kieserit pada bibit kakao. Hasil yang diperoleh dari penelitian ini mengindikasikan bahwa kadar MgO total dan kelarutan dalam air pada dolomit teraktivasi masing-masing 26,7 dan 9,2%. Dengan menggunakan bibit kakao sebagai tanaman uji, aplikasi 3,85 g dolomit teraktivasi yang diperkaya dengan 109 cfu Pseudomonas sp./bibit yang dikombinasikan dengan 100% dosis pupuk NPK menunjukkan pertumbuhan vegetatif paling baik dan total bobot kering bibit kakao yang secara nyata lebih tinggi jika dibandingkan dengan pemberian 100% dosis standar NPK-Mg kiserit. 

scholarly journals Evaluation of Potential Native Phosphate Solubilizing Bacteria for the Agriculture Practice of okra (Abelmoschus Esculentus) with the Target to Replace Chemical Fertilizer - in a Field Study

2019 ◽  
Vol 7 (4.14) ◽  
pp. 40
Author(s):  
P Sajeesh ◽  
P Paulraj ◽  
V Jaamuna ◽  
N Jayakumar ◽  
K P Sajna ◽  
...  
Keyword(s):  
Rock Phosphate ◽  
Growth Parameters ◽  
Plant Root ◽  
Chemical Fertilizer ◽  
Phosphate Solubilizing Bacteria ◽  
Agricultural Industry ◽  
Limiting Nutrient ◽  
Number Of Leaves ◽  
The Difference ◽  
Phosphate Solubilizing

Phosphorus (P) is a major growth limiting nutrient. Phosphate Solubilizing Bacteria (PSB) plays an important role in the development of agricultural industry. In this study, the native PSB were isolated from the okra plant root by using Pikovskaya’s medium and potential strains were selected based on the phosphate solubilization efficiency. The potential strains in presence of Rock Phosphate (RP), Tea dust, Eggshells and Bone waste of animal (TEB) were studied as an alternative fertilizer instead of chemical fertilizer. The PSB consortia strains were prepared and inoculated to the plant root. Different growth parameters were studied to determine the effectiveness of strains. In all cases the difference was significant with p<0.05. A decreasing trend of results were recorded among the tested parameters from plants in PSB with RP, PSB with TEB, and PSB with TSP and followed by PSB without fertilizer. The highest results were recorded among plants in PSB with RP for mean shoot length (64cm), mean weight of fruits (29gm), mean length of fruits (23cm), mean number of leaves (13Nos) and chlorophyll content (5.1mg/g). Hence, the present study showed that the application of PSB with the cheap source of nutrients has shown the capability of providing similar or enhanced results based on the various characters expressed by the plant and thus reduce the reliance on synthetic chemical fertilizers. 

scholarly journals Efektivitas dolomit teraktivasi yang diperkaya dengan bakteri pelarut fosfat sebagai pengganti kiserit pada bibit kakao The effectiveness of activated dolomite enriched by phosphate solubilizing bacteria as kieserite substitute on cocoa seedling

2016 ◽  
Vol 80 (1) ◽  
Author(s):  
Laksmita Prima SANTI ◽  
Didiek Hadjar GOENADI
Keyword(s):  
Acid Soils ◽  
Dry Mass ◽  
Test Plant ◽  
Phosphate Solubilizing Bacteria ◽  
Pseudomonas Sp ◽  
Npk Fertilizers ◽  
Standard Dosage ◽  
Tropical Regions ◽  
Mg Deficiency ◽  
Phosphate Solubilizing

Abstract Magnesium (Mg) deficiency is commonly found in acid soils with high weathering intensity as widely present in tropical and sub-tropical regions, or those developed from low Mg-content parent materials. To overcome Mg deficiency, dolomite is often used to replace kieserite although the former is less reactive than the later. An activation technology by heating up to 800oC, followed by sulfic acid addition, then enriched by phosphate solubilizing bacteria is prospective to improve Mg solubility in soils. This research was aimed to enhance dolomite reactivity and to determine the effectiveness of activated dolomite enriched by phosphate solubilizing bacteria as Mg nutrient sources substituting kieserite on cocoa seedling growth. The results indicate that total and solubility in water of MgO from the prototype of activated dolomite were 26.7 and 9.2%, respectively. Using cocoa seedling as test plant, the use of activated dolomite enriched by using 109 cfu of Pseudomonas sp. at 3.85 g/seedling and combined with 100% dosages of NPK fertilizers showed the best vegetative growth and total dry mass of seedlings was significantly higher than that of 100% NPK-Mg kieserite standard dosage Abstrak Defisiensi Mg umumnya ditemukan pada tanah masam dengan tingkat pelapukan tinggi yang banyak terdapat di daerah tropik, subtropik, dan tanah yang terbentuk dari bahan induk miskin Mg. Untuk mengatasi defisiensi Mg, dolomit sering digunakan sebagai pengganti kiserit walaupun sifatnya kurang reaktif jika dibandingkan kiserit. Teknologi aktivasi dengan pemanasan mencapai 800oC, dilanjutkan dengan penambahan asam sulfat serta pengkayaan dengan bakteri pelarut fosfat diharapkan dapat meningkatkan kelarutan Mg di dalam tanah. Penelitian ini bertujuan untuk meningkatkan reaktivitas dolomit serta menetapkan efektivitas dolomit teraktivasi yang diperkaya dengan bakteri pelarut fosfat sebagai sumber hara Mg pengganti kieserit pada bibit kakao. Hasil yang diperoleh dari penelitian ini mengindikasikan bahwa kadar MgO total dan kelarutan dalam air pada dolomit teraktivasi masing-masing 26,7 dan 9,2%. Dengan menggunakan bibit kakao sebagai tanaman uji, aplikasi 3,85 g dolomit teraktivasi yang diperkaya dengan 109 cfu Pseudomonas sp./bibit yang dikombinasikan dengan 100% dosis pupuk NPK menunjukkan pertumbuhan vegetatif paling baik dan total bobot kering bibit kakao yang secara nyata lebih tinggi jika dibandingkan dengan pemberian 100% dosis standar NPK-Mg kiserit. 

scholarly journals Bioinoculant production composed by Pseudomonas sp., Serratia sp., and Kosakonia sp., preliminary effect on Allium cepa L., growth at plot scale

2021 ◽  
Vol 26 (1) ◽  
pp. 79-118
Author(s):  
Andrea Blanco-Vargas ◽  
Lina M Rodríguez-Gacha ◽  
Natalia Sánchez-Castro ◽  
Laura Herrera-Carlosama ◽  
Raúl A. Poutou-Piñales ◽  
...  
Keyword(s):  
Low Cost ◽  
Mineral Fertilizer ◽  
Dry Weight ◽  
Phosphate Solubilizing Bacteria ◽  
Pseudomonas Sp ◽  
Yeast Hydrolysate ◽  
Organic Mineral ◽  
Allium Cepa L ◽  
Essential Nutrient ◽  
Phosphate Solubilizing

Phosphorus (P) is an essential nutrient for plant’s development, and its deficiency restricts crop yield. To meet P requirements in agricultural settings, a low-cost culture medium (MT11B) was designed in which a bioinoculant was produced consisting of three bacterial isolates capable of solubilizing P from phosphoric rock (PR). Pseudomonas sp., Serratia sp., and Kosakonia sp. exhibited P solubilization in SMRS1 agar modified with PR (5.0 g L-1), as source of inorganic P. Sowings by isolation were made of the three bacteria on DNAse- and Blood-agar to rule out pathogenicity. At the interaction tests, no inhibition halos were observed; demonstrating there was no antagonism among them, thus they were used to constitute a consortium. Growth curve (12 h) in MT11B demonstrated consortium grew in presence of PR, brewer’s yeast hydrolysate, and glucose at concentrations (2.5 g L-1) fourfold lower than those in SMRS1 (10.0 g L-1); obtaining phosphate solubilizing bacteria of (10.60 ± 0.08/ log10 CFUmL-1 and, at 6 h of culture, acid and alkaline phosphatase enzyme volumetric activities of 2.3 ± 0.8 and (3.80 ± 0.13) UP, respectively. The consortium, releasing phosphorus at a rate of (45.80 ± 5.17) mg L-1 at 6 h of production, was evaluated as bioinoculant in onion plots for five months. Plants receiving a treatment that included 500 mL (10 x 107 CFU mL-1) of bioinoculant plus 100 kg ha-1 of an organic mineral fertilizer exhibited the highest determined response variables (170.1 ± 22.2) mm bulb height, (49.4 ± 6.5) mm bulb diameter, (9.0 ± 1.8) g bulb dry weight, and 15.21 mg bulb-1 total phosphorus (p < 0.05).

scholarly journals ​​Eco-friendly Management of Collar Rot of Lentil by Introduced Native Rhizobacterial Candidates

2021 ◽  
Author(s):  
Amitava Mondal ◽  
Sunita Mahapatra ◽  
Sunanda Chakraborty ◽  
Debanjana Debnath ◽  
Tanusree Das ◽  
...  
Keyword(s):  
Disease Management ◽  
Growth Parameters ◽  
Management Strategies ◽  
Sclerotium Rolfsii ◽  
Plant Growth Promoting Rhizobacteria ◽  
Phosphate Solubilizing Bacteria ◽  
Collar Rot ◽  
Plant Growth Promoting ◽  
Phosphate Solubilizing

Background: Collar rot is an important disease of lentil in India and causes significant yield loss annually. Considering the recent focus on the development and use of environmentally feasible management strategies, the objectives of the study was to identify resistant sources and evaluation of native antagonists as well as plant growth promoting rhizobacteria (PGPRs) in yield improvement and disease management of lentil. Methods: Eleven popular lentil varieties were screened for resistance/susceptibility reaction against collar rot in vivo. The efficacy of two bacterial and fungal biocontrol agents (BCAs) was tested against a virulent isolate of Sclerotium rolfsii. Four PGPRs were also evaluated to study their influence on the growth parameters as well as their ability to manage S. rolfsii. Result: Three genotypes were found to be tolerant, four genotypes were moderately susceptible, while four genotypes were highly susceptible. Among the BCAs, the highest average inhibition % was observed in treatment with Bacillus sp. Among the PGPR treatments, Rhizobium in combination with phosphate solubilizing bacteria and Trichoderma or Bacillus was the most effective in controlling the collar rot when used as seed treatment and hence can be used for disease management.

scholarly journals SISTEM KULTIVASI ORGANIK PADA KACANG TANAH (Arachis hypogea L.) DENGAN MEMANFAATKAN TINGKAT KEMATANGAN KOMPOS

2016 ◽  
Vol 13 (2) ◽  
pp. 203
Author(s):  
Dwi Agustiyani ◽  
Budhi Martono ◽  
Maman Rahmansyah
Keyword(s):  
Plant Growth ◽  
Organic Substrate ◽  
Phosphate Solubilizing Bacteria ◽  
Pseudomonas Sp ◽  
Arachis Hypogea ◽  
Manure Compost ◽  
Trichoderma Sp ◽  
Rhizobium Spp ◽  
Aspergillus Sp ◽  
Phosphate Solubilizing

Untuk mendukung suatu sistem pertanian yang berkelanjutan, praktek pertanian organic dilakukan pada kultivasi kacang tanah. Pada penelitian ini, pengkombinasian antara penggunaan bakteri pelarut fosfat (Pseudomonas sp.), bakteri penambat nitrogen (Rhizobium spp.), dan fungi pendegradasi selulosa (Trichoderma sp. dan Aspergillussp.) digunakan untuk inokulan. Kompos berupa bahan hijauan daun dan limbah kotoran ternak kemudian diformulasikan dengan inokulan untuk digunakan sebagai pupuk hayati. Kompos-matang dan kompos-setengah-matang fermentasi digunakan untuk pengkayaan hara tanah. Penggunaan kompos setengah matang merupakanpraktek semi-on-site-composting di dalam upaya menstimulasi pertum-buhan tanaman. Pengaruh perlakuan pada penelitian ini dievaluasi melalui pertum-buhan tanaman dan hasil polong. Tidak didapat perbedaan yang signifikan di antara perlakuan pupuk hayati, namun seluruh perlakuan memperlihatkan perbedaan yang nyata terhadap kontrol.Oleh sebab itu, praktek pengunaan kompos setengah matang mampu mempersingkat persiapan tanam dan dapat diterapkan pada kultivasi kacang tanah. Penggunaan kompos separuh matang hasil penelitian ini dapat dipraktekan di dalam pengembangan tehnik kultivasi untuk mendukung sistem pertanian berkelanjutan. Kata kunci : pupuk hayati, semi-on-site-composting, sistem pertanian berkelanjutan, Arachis hypogea L. AbstractIn order to support a sustainable agriculture system, an organic farming purpose had been carried out all through groundnut cultivation. In this experiment, the combination of phosphate solubilizing bacteria (Pseudomonas sp.), fixing nitrogen bacteria (Rhizobium spp.), and fungal degrading cellulose (Trichoderma sp. and Aspergillus sp.) were used as inoculant. Organic substrate as a green manure compost and cattle waste was mixing with the inoculant to utilize into biofertilizer improvement. Full and half fermented compost materials designed for soil augmentation. Half fermented compost materials exploited to semi-on-site-composting practice in appropriate to fuel plant growth. Result of the treatment evaluated through plant growth and the pods yield. There have not significant  different among biofertilizer handling, and all have the exclusion to the control. As due

scholarly journals Influence of an Arbuscular Mycorrhizal Fungus and Phosphate-Solubilizing Bacterium Inoculation at Stem Cutting Stage on P Uptake and Growth of Impatiens walleriana Plants in an Unsterile Field Soil

2019 ◽  
Vol 27 (2) ◽  
pp. 11-22
Author(s):  
Muthukumar Thangavelu ◽  
Prabhu Arumugam
Keyword(s):  
Growth Parameters ◽  
Arbuscular Mycorrhizal ◽  
Am Fungi ◽  
P Uptake ◽  
Phosphate Solubilizing Bacteria ◽  
Stem Cuttings ◽  
Field Soil ◽  
Dual Inoculation ◽  
Impatiens Walleriana ◽  
Phosphate Solubilizing

AbstractMicroorganisms play an important role in the propagation and growth of plants. Propagation of plants through stem cuttings is a popular method widely practiced in horticulture and forestry. As the information on the interactive role of arbuscular mycorrhizal (AM) fungi and phosphate-solubilizing bacteria (PSB) in the regeneration and growth of clonally propagated plants is limited, we inoculated stem cuttings of Impatiens walleriana with Rhizophagus fasciculatus and Bacillus megaterium var. phosphaticum individually or in combination in an unsterile Alfisol field soil. The inoculated cuttings were harvested after 45 days of cultivation and various growth parameters and phosphorus (P) uptake were measured. Inoculation with the AM fungus or PSB individually improved plant growth parameters, whereas the dual inoculation did not give synergistic results. Plants inoculated at cutting stage with R. fasciculatus accumulated the highest plant biomass and showed maximum microbial inoculation effect and efficiency of P uptake. Inoculation with the PSB stimulated symbiosis with native AM fungi but a synergistic effect has not been found when both AM fungi and PSB were co-inoculated. It could be concluded that screening for compatibility between microorganisms is essential before selecting the bioinoculants for dual inoculation.

Sign in / Sign up

Export Citation Format

Share Document