scholarly journals Rapid detection of Salmonella sp. in pork samples using fluorescent in situ hybridization: a comparison with VIDAS®-SLM system and ISO 6579 cultural method

2007 ◽  
Vol 59 (6) ◽  
pp. 1388-1393 ◽  
Author(s):  
M. Vieira-Pinto ◽  
M. Oliveira ◽  
F. Bernardo ◽  
C. Martins
Keyword(s):  
In Situ Hybridization ◽  
Rapid Detection ◽  
Fluorescent In Situ Hybridization ◽  
Diagnostic Assay ◽  
Promising Tool ◽  
Cultural Method ◽  
Inner Surface ◽  
Peptone Water ◽  
Pork Samples

This study reports the use of the fluorescent in situ hybridization (FISH) with Sal3 probe for Salmonella detection in swine carcasses inner surface (swab); and in the correspondent samples of ileum, ileocolic, and mandibular lymph nodes; and tonsils, after dilution (1:10) in buffered peptone water and a pre-enrichment step (37(0)C, 18h). In order to evaluate the efficiency of FISH, 235 naturally contaminated samples were simultaneously tested by the cultural method (ISO 6579) and by the Vitek Immuno Diagnostic Assay System (VIDAS®) - Salmonella (SLM) system. The cultural method identified 39 positive samples. From these, VIDAS®- SLM only detected 23. FISH identified 115 positive samples. This difference was highly significant (P<0.001). From positive samples, 32 were also confirmed by the cultural method. The results indicate FISH as a promising tool for rapid Salmonella detection in samples of pork and swine carcasses.

scholarly journals Specific and Rapid Detection by Fluorescent In Situ Hybridization of Bacteria in Clinical Samples Obtained from Cystic Fibrosis Patients

2000 ◽  
Vol 38 (2) ◽  
pp. 818-825 ◽  
Author(s):  
Michael Hogardt ◽  
Karlheinz Trebesius ◽  
Anna M. Geiger ◽  
Mathias Hornef ◽  
Josef Rosenecker ◽  
...  
Keyword(s):  
Cystic Fibrosis ◽  
In Situ Hybridization ◽  
Rapid Detection ◽  
Burkholderia Cepacia ◽  
Fluorescent In Situ Hybridization ◽  
Clinical Samples ◽  
Microbial Culture ◽  
Specific Detection ◽  
Pulmonary Exacerbations

We report on the rapid and specific detection of bacteria commonly isolated from clinical specimens from cystic fibrosis (CF) patients by fluorescent in situ hybridization (FISH). On the basis of comparative sequence analysis, we designed oligonucleotide probes complementary to species-specific 16S rRNA regions of these microorganisms and demonstrated the specificities of the probes by hybridization of different remotely related as well as closely related reference strains. Furthermore, in a pilot project we investigated 75 sputum samples and 10 throat swab specimens from CF patients by FISH and detected Pseudomonas aeruginosa, Burkholderia cepacia, Stenotrophomonas maltophilia,Haemophilus influenzae, and Staphylococcus aureus within these specimens. The specificity of FISH was 100% in comparison to the results of conventional microbial culture. In contrast, the sensitivity of standard laboratory cultivation was moderately higher, since the limit for microscopic detection of bacteria within sputum samples by FISH was approximately 4 × 105 CFU/ml of sputum (resulting in a 90% sensitivity for FISH). Moreover, we demonstrated that FISH will be useful for the rapid detection of bacteria that cause acute pulmonary exacerbations in CF patients, as demonstrated in patients with H. influenzae, S. aureus, andP. aeruginosa exacerbations. Therefore, FISH is a valuable additional method for the rapid and specific detection of bacteria in clinical samples from CF patients, in particular, patients with pulmonary exacerbations.

Influence of an enrichment step on Salmonella sp. detection by fluorescent in situ hybridization on pork samples

Food Control ◽  
2008 ◽  
Vol 19 (3) ◽  
pp. 286-290 ◽  
Author(s):  
Madalena Vieira-Pinto ◽  
Manuela Oliveira ◽  
José Aranha ◽  
Conceição Martins ◽  
Fernando Bernardo
Keyword(s):  
In Situ Hybridization ◽  
Fluorescent In Situ Hybridization ◽  
Pork Samples ◽  
Enrichment Step

scholarly journals DNA FISH Diagnostic Assay on Cytological Samples of Thyroid Follicular Neoplasms

Cancers ◽  
2020 ◽  
Vol 12 (9) ◽  
pp. 2529
Author(s):  
Philippe Vielh ◽  
Zsofia Balogh ◽  
Voichita Suciu ◽  
Catherine Richon ◽  
Bastien Job ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescent In Situ Hybridization ◽  
Low Cost ◽  
Needle Aspiration ◽  
Molecular Techniques ◽  
Comparative Genomic ◽  
Diagnostic Assay ◽  
Clinical Value ◽  
Needle Aspiration Cytology

Although fine-needle aspiration cytology (FNAC) is helpful in determining whether thyroid nodules are benign or malignant, this distinction remains a cytological challenge in follicular neoplasms. Identification of genomic alterations in cytological specimens with direct and routine techniques would therefore have great clinical value. A series of 153 cases consisting of 72 and 81 histopathologically confirmed classic follicular adenomas (cFAs) and classic follicular thyroid carcinomas (cFTCs), respectively, was studied by means of different molecular techniques in three different cohorts of patients (pts). In the first cohort (training set) of 66 pts, three specific alterations characterized by array comparative genomic hybridization (aCGH) were exclusively found in half of cFTCs. These structural abnormalities corresponded to losses of 1p36.33-35.1 and 22q13.2-13.31, and gain of whole chromosome X. The second independent cohort (validation set) of 60 pts confirmed these data on touch preparations of frozen follicular neoplasms by triple DNA fluorescent in situ hybridization using selected commercially available probes. The third cohort, consisting of 27 archived cytological samples from an equal number of pts that had been obtained for preoperative FNAC and morphologically classified as and histologically verified to be follicular neoplasms, confirmed our previous findings and showed the feasibility of the DNA FISH (DNA fluorescent in situ hybridization) assay. All together, these data suggest that our triple DNA FISH diagnostic assay may detect 50% of cFTCs with a specificity higher than 98% and be useful as a low-cost adjunct to cytomorphology to help further classify follicular neoplasms on already routinely stained cytological specimens.

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