Library Construction Protocols v1

2021 ◽  
Author(s):  
Graham J Etherington ◽  
Darren Heavens ◽  
David Baker ◽  
Ashleigh Lister ◽  
Rose McNelly ◽  
...  
Keyword(s):  
Library Construction

Amplification Free Paired End Library Construction Protocol.

scholarly journals Validation and standardization of DNA extraction and library construction methods for metagenomics-based human fecal microbiome measurements

Microbiome ◽  
2021 ◽  
Vol 9 (1) ◽  
Author(s):  
Dieter M. Tourlousse ◽  
Koji Narita ◽  
Takamasa Miura ◽  
Mitsuo Sakamoto ◽  
Akiko Ohashi ◽  
...  
Keyword(s):  
Dna Extraction ◽  
High Throughput Sequencing ◽  
Performance Metrics ◽  
Collaborative Study ◽  
Human Microbiome ◽  
Second Phase ◽  
Intermediate Precision ◽  
Fecal Microbiome ◽  
Library Construction ◽  
Wide Range

Abstract Background Validation and standardization of methodologies for microbial community measurements by high-throughput sequencing are needed to support human microbiome research and its industrialization. This study set out to establish standards-based solutions to improve the accuracy and reproducibility of metagenomics-based microbiome profiling of human fecal samples. Results In the first phase, we performed a head-to-head comparison of a wide range of protocols for DNA extraction and sequencing library construction using defined mock communities, to identify performant protocols and pinpoint sources of inaccuracy in quantification. In the second phase, we validated performant protocols with respect to their variability of measurement results within a single laboratory (that is, intermediate precision) as well as interlaboratory transferability and reproducibility through an industry-based collaborative study. We further ascertained the performance of our recommended protocols in the context of a community-wide interlaboratory study (that is, the MOSAIC Standards Challenge). Finally, we defined performance metrics to provide best practice guidance for improving measurement consistency across methods and laboratories. Conclusions The validated protocols and methodological guidance for DNA extraction and library construction provided in this study expand current best practices for metagenomic analyses of human fecal microbiota. Uptake of our protocols and guidelines will improve the accuracy and comparability of metagenomics-based studies of the human microbiome, thereby facilitating development and commercialization of human microbiome-based products.

cDNA library construction of two human Demodexspecies

2017 ◽  
Vol 62 (2) ◽  
Author(s):  
DongLing Niu ◽  
RuiLing Wang ◽  
YaE Zhao ◽  
Rui Yang ◽  
Li Hu ◽  
...  
Keyword(s):  
Cdna Library ◽  
Cdna Library Construction ◽  
Library Construction

AbstractThe research of

scholarly journals Capturing native long-range contiguity by in situ library construction and optical sequencing

2012 ◽  
Vol 109 (46) ◽  
pp. 18749-18754 ◽  
Author(s):  
J. J. Schwartz ◽  
C. Lee ◽  
J. B. Hiatt ◽  
A. Adey ◽  
J. Shendure
Keyword(s):  
Long Range ◽  
Library Construction

scholarly journals Library Construction from Subnanogram DNA for Pelagic Sea Water and Deep-Sea Sediments

2017 ◽  
Vol 32 (4) ◽  
pp. 336-343 ◽  
Author(s):  
Miho Hirai ◽  
Shinro Nishi ◽  
Miwako Tsuda ◽  
Michinari Sunamura ◽  
Yoshihiro Takaki ◽  
...  
Keyword(s):  
Deep Sea ◽  
Sea Water ◽  
Library Construction ◽  
Deep Sea Sediments

Complementary DNA library construction and expressed sequence tag analysis of an Arctic moss, Aulacomnium turgidum

Polar Biology ◽  
2009 ◽  
Vol 33 (5) ◽  
pp. 617-626 ◽  
Author(s):  
Shenghao Liu ◽  
Hyoungseok Lee ◽  
Pil-Sung Kang ◽  
Xiaohang Huang ◽  
Joung Han Yim ◽  
...  
Keyword(s):  
Expressed Sequence Tag ◽  
Complementary Dna ◽  
Library Construction ◽  
Dna Library ◽  
Expressed Sequence Tag Analysis ◽  
Expressed Sequence ◽  
Aulacomnium Turgidum

scholarly journals Fosmid library construction and screening for the maize mutant gene Vestigial glume 1

2016 ◽  
Vol 4 (1) ◽  
pp. 55-60 ◽  
Author(s):  
Chaoxian Liu ◽  
Xiaoli Liu ◽  
Lei Lei ◽  
Haiying Guan ◽  
Yilin Cai
Keyword(s):  
Mutant Gene ◽  
Fosmid Library ◽  
Library Construction
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