scholarly journals AN IN VITRO BIOASSAY FOR THE EVALUATION OF COLD TREATMENT ON FLOWER BUD DORMANCY IN CAMELLIA

2012 ◽  
pp. 607-611
Author(s):  
A. Berruti ◽  
A. Christiaens ◽  
M.C. Van Labeke ◽  
V. Scariot
Keyword(s):  
Cold Treatment ◽  
Bud Dormancy ◽  
In Vitro Bioassay ◽  
Flower Bud

Effects of Theobroxide, a Natural Product, on the Level of Endogenous Jasmonoids

2004 ◽  
Vol 59 (11-12) ◽  
pp. 828-834 ◽  
Author(s):  
Qing Yang ◽  
Xiquan Gao ◽  
Yumiko Fujino ◽  
Hideyuki Matsuura ◽  
Teruhiko Yoshihara
Keyword(s):  
Solanum Tuberosum L ◽  
Morning Glory ◽  
Pharbitis Nil ◽  
In Vitro Bioassay ◽  
Flower Bud ◽  
Single Node ◽  
Whole Plant ◽  
Tuberonic Acid ◽  
Short Days

The natural potato microtuber inducing substance, theobroxide, strongly induces the formation of tuber of potato (Solanum tuberosum L.) and flower bud of morning glory (Pharbitis nil) plants under non-inducing conditions (long days) (Yoshihara et al., 2000). In the present study, theobroxide was evaluated for its effect on the level of endogenous jasmonoids in different tissues of such two plants. An in vitro bioassay using cultures of single-node segments of potato stems was performed with the supplement of theobroxide in the medium. The endogenous jasmonic acid (JA) and its analogue tuberonic acid (TA, 12-hydroxyjasmonic acid) in segments and microtubers were quantitatively analyzed. The increase in the endogenous JA level caused by theobroxide was observed in both segments and microtubers. Endogenous TA was only detected in segments, and the content increased with the concentration of theobroxide. As for morning glory, the whole plant was sprayed with theobroxide for 1 ~ 5 weeks under different photoperiods and endogenous JA in the leaves was quantitatively analyzed. Theobroxide spraying increased the level of endogenous JA in the leaves of the plants grown under both long and short days.

scholarly journals Efficiency of Deoxynivalenol Detoxification by Microencapsulated Sodium Metabisulfite Assessed via an In Vitro Bioassay Based on Intestinal Porcine Epithelial Cells

ACS Omega ◽  
2021 ◽  
Vol 6 (12) ◽  
pp. 8382-8393
Author(s):  
Changning Yu ◽  
Peng Lu ◽  
Shangxi Liu ◽  
Qiao Li ◽  
Erhua Xu ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Sodium Metabisulfite ◽  
In Vitro Bioassay

Bioactivity of human growth hormone in serum: validation of an in vitro bioassay.

Endocrinology ◽  
1993 ◽  
Vol 132 (5) ◽  
pp. 2073-2082 ◽  
Author(s):  
C M Foster ◽  
M Borondy ◽  
V Padmanabhan ◽  
J Schwartz ◽  
G B Kletter ◽  
...  
Keyword(s):  
Growth Hormone ◽  
Human Growth Hormone ◽  
Human Growth ◽  
In Vitro Bioassay

scholarly journals An in vitro bioassay to assess the potential global toxicity of waters on spermatogenesis: a pilot study

2021 ◽  
Author(s):  
Antonine Blondet ◽  
Guillaume Martin ◽  
Laurent Paulic ◽  
Marie-Hélène Perrard ◽  
Philippe Durand
Keyword(s):  
Pilot Study ◽  
In Vitro Bioassay

Bactericidal and Fungicidal Activities of Calia secundiflora (Ort.) Yakovlev

2008 ◽  
Vol 63 (9-10) ◽  
pp. 653-657 ◽  
Author(s):  
Dolores Pérez-Laínez ◽  
Rosario García-Mateos ◽  
Ruben San Miguel-Chávez ◽  
Marcos Soto-Hernández ◽  
Enrique Rodríguez-Pérez ◽  
...  
Keyword(s):  
Xanthomonas Campestris ◽  
Alternaria Solani ◽  
Erwinia Carotovora ◽  
Phytopathogenic Fungi ◽  
Quinolizidine Alkaloids ◽  
Liquid Chromatography Mass Spectrometry ◽  
In Vitro Bioassay ◽  
Organic Extract ◽  
Phytopathogenic Fungi And Bacteria

Calia secundiflora (Ortega) Yakovlev (Fabaceae) is considered a medicinal plant in Mexico but has scarcely been used because of the toxicity of its quinolizidine alkaloids. Several quinolizidine alkaloids have shown bactericidal, nematicidal, and fungicidal activities. The purpose of this study was to identify the alkaloids in the seeds and evaluate the activity of the organic extract on several phytopathogenic fungi and bacteria. An in vitro bioassay was conducted with species of the following phytopathogenic fungi: Alternaria solani, Fusarium oxysporum and Monilia fructicola; and of the following bacteria Pseudomonas sp., Xanthomonas campestris and Erwinia carotovora. Cytisine, lupinine, anagyrine, sparteine, N-methylcytisine, 5,6-dehydrolupanine, and lupanine were identified by liquid chromatography-mass spectrometry in the extract of seeds; the most abundant compound of the extract was cytisine. It was observed that the crude extract of Calia secundiflora was moderately active on bacteria and more potent on phytopathogenic fungi. In contrast cytisine showed the opposite effects.

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