scholarly journals Biochemical changes in blood serum of rats with experimental burn disease and their correction with melanin

2016 ◽  
Vol 21 (2) ◽  
pp. 44-48
Author(s):  
N. Chornenka ◽  
Ya. Rayetska ◽  
O. Savchuk ◽  
L. Ostapchenko
Keyword(s):  
Blood Serum ◽  
Total Protein ◽  
Biochemical Parameters ◽  
Electrolyte Balance ◽  
Experimental Burn ◽  
Metabolic Systems ◽  
Immature Rats ◽  
Urea Metabolism ◽  
Activity Of Enzymes ◽  
Burn Disease

During modeling of esophageal alkali burns 1 and 2 degrees in immature rats were observed violations of various metabolic systems, melanin administration helped to normalize the biochemical parameters. It was shown that under the esophageal burns there occurred violations of protein (total protein, albumin), nitrogen metabolism (creatinine, urea) metabolism of carbohydrate (glucose) and lipids (cholesterol), changed the activity of enzymes (GOT, GPT, CC), violated fluid and electrolyte balance (K +, Na +, Cl-, Ca2 +, Mg2 +, phosphates), while the introduction of melanin contributed to the normalization of these indicators. The most effective dose were of 0.5 mg/ kg and 1 mg/kg. There were found anti-toxic properties of melanin, which may be a promising drug for the normalization of metabolism in patients with burns of esophagus and prevent its complications.

scholarly journals Biochemical changes in blood serum of rats with experimental esophageal burn II degree under treatment with aqueous extract of P. vulgaris pods

2017 ◽  
Vol 23 (2) ◽  
pp. 14-19
Author(s):  
V. Dmytryk ◽  
Ya. Raetska
Keyword(s):  
Phaseolus Vulgaris ◽  
Blood Serum ◽  
Therapeutic Effect ◽  
Aqueous Extract ◽  
Total Protein ◽  
Electrolyte Balance ◽  
Metabolic Systems ◽  
Immature Rats ◽  
Activity Of Enzymes ◽  
Esophageal Burn

During modeling of esophageal alkali burns II degree in immature rats were observed violations of various metabolic systems. It was shown that under the esophageal burns there occurred violations of protein (total protein, albumin), nitrogen metabolism (creatinine, urea), changed the activity of enzymes (GOT, GPT), violated fluid and electrolyte balance (Na+, К+, Cl-, Ca2+, Mg2+). In the groups of animals with the alkaline burn of the esophagus, that were injected aqueous extract of pod of bean (Phaseolus vulgaris) there can be traced a change of the indicators in the direction of normalization, indicating about the obvious therapeutic effect of aqueous extract of pod of bean (Phaseolus vulgaris).

scholarly journals The effect of camel milk curd masses on rats blood serum biochemical parameters: Preliminary study

PLoS ONE ◽  
2021 ◽  
Vol 16 (9) ◽  
pp. e0256661
Author(s):  
Fatima Dikhanbayeva ◽  
Elmira Zhaxybayeva ◽  
Zhuldyz Smailova ◽  
Arman Issimov ◽  
Zhechko Dimitrov ◽  
...  
Keyword(s):  
Blood Serum ◽  
Total Protein ◽  
Biochemical Parameters ◽  
Camel Milk ◽  
Control Group ◽  
Serum Biochemical Parameters ◽  
Group V ◽  
Group I ◽  
Serum Biochemical ◽  
Group Ii

This study aimed to assess potential feeding effect of camel milk curd mass and its mixes to experimental rat’s blood serum biochemical parameters, enzymatic activity and the peptide toxicity. Fifty healthy male Sprague-Dawley rats were divided into five groups (n = 10 each). Each group was fed with camel milk pure curd mass and its mixes for 16 days. At the end of the experiment, rats were sacrificed to collect the samples from the blood serum. Blood serum biochemical parameters total protein, cholesterol, glucose, albumin, triglycerides; the enzymatic activities of alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase were determined on the A25 automatic analyser, and peptide toxicity analysed by the reference method. The statistical data have shown no significant differences in body weight gain in all groups. Total protein decreased in group II, IV, and V; however, it increased in group III compared to the control group. Cholesterol grew up in group II and it slightly increased in group V, dropped in groups III and IV compared to group I result. Glucose increased in groups II, III, IV compared to group I; still, group V results show a slight decrease. Albumin decreased in group IV, yet in group V it increased than the group I result. Simultaneously, groups II and III results were changed with less percentage. Triglyceride grew up in groups II, V, and it dropped significantly in groups III, IV compared to the control group. De Ritis ratio of enzymes in groups II, III, and IV fluctuated between 1.31 and 0.98 IU/L; however, group V demonstrated significant data versus group I. Diets peptide toxicity in all groups was lower than control group data. The experimental results indicated that curd mass from camel milk could be used as a pure or with additives and it did not discover the observed side effects.

scholarly journals Use of Ovostim-CT in the prevention of hepatosis and retention of placenta in cows after calving

2021 ◽  
Vol 282 ◽  
pp. 03014
Author(s):  
P.V. Burkov
Keyword(s):  
Blood Serum ◽  
Liver Damage ◽  
Total Protein ◽  
Biochemical Parameters ◽  
Live Weight ◽  
Favorable Effect ◽  
Pathological Examination ◽  
Milk Productivity

High milk productivity of cows after calving is the cause of liver damage in the form of hepatosis and premature retirement of animals. In such cases, a pathological examination simultaneously reveals violations in the form of protein and fatty degenerations with the subsequent destruction of cells, as well as disturbed circulation in the liver. Also, hepatoses can cause such postpartum complications in cows as retention of placenta. For the prevention of hepatosis, the drug “Ovostim-ct” is proposed, which contains cytotoxic sera against the tissues of the liver, ovary, spleen and muscle. In order to prevent liver damage and postpartum retention of placenta, the drug is used 1-1.5 months before calving at a dose of 1 ml per 100 kg of live weight subcutaneously or intramuscularly. The use of the hepatoprotector allowed to reduce the number of cases of hepatosis and retention of placenta in cows after calving. Also, “Ovostim-ct” had a favorable effect on the biochemical parameters of the blood serum of cows: there was an increase in the concentration of total protein by 6.7%, albumin - by 15.6% and glucose - by 45.8% in the blood.

scholarly journals Prediction of birth and postpartum pathology in deep-calving heifers by biochemical parameters of blood

2021 ◽  
Vol 23 (101) ◽  
pp. 21-25
Author(s):  
L. V. Koreyba
Keyword(s):  
Blood Serum ◽  
Total Protein ◽  
Biochemical Parameters ◽  
Mineral Metabolism ◽  
Obstetric Care ◽  
Calcium Content ◽  
Inorganic Phosphorus ◽  
The State ◽  
Total Protein Content ◽  
Carotene Content

The success of the control of obstetric diseases in cattle depends on the timely implementation of preventive measures, and, in cases of pathology, on diagnosis, effective obstetric care and treatment. A prominent place is given to blood tests among the methods that allow to objectively assess the state of health and the course of the pathological process in animals' bodies. The study of biochemical parameters of blood allows for the assessment of the state of metabolic processes in animals, with high accuracy in establishing the body's general condition and predicting complications and adjusting their prevention and therapy. The goal of the work: our work aimed to study the biochemical parameters of the blood of deep-calving heifers for the prediction, correction, and prevention of obstetric pathology during childbirth and puerperium. The research was carried out on deep-calving heifers of Holstein breed selected on the principle of analogues. Biochemical examination of blood serum was performed in the district laboratory of veterinary medicine. Indicators of protein and mineral metabolism were determined in the blood serum of deep- calving heifers: total protein, carotene, reserve alkalinity, inorganic phosphorus, albumin content, class M immunoglobulins. The obtained research results indicate that the total protein content corresponds to the norm in deep-calving heifers of the Holstein black-spotted breed. The protein coefficient exceeds the standard. There are pronounced deviations in the blood's biochemical parameters: low levels of carotene almost two-fold, reserve alkalinity – 1.91-fold, the total calcium content – by 16.08 % and the ratio of calcium to phosphorus by 17.78 %. Since the decrease in carotene content correlates with the predisposition to endometritis, the Holstein black-spotted breed's deep-calving heifers are prone to developing birth and postpartum pathology. For pregnant cows which are predisposed to postpartum obstetric pathology, that occur in weak parturition activity, failure to release the placenta, uterus subinvolution, endometritis and postpartum hypocalcaemia, unfavorable prognostication is the low concentration in blood plasma of general calcium, inorganic phosphorus and carotene.

scholarly journals The impact of endogenous intoxication on biochemical indicators of blood of cows with calves

2017 ◽  
Vol 8 (3) ◽  
pp. 438-443 ◽  
Author(s):  
B. Gutyj ◽  
Y. Grymak ◽  
M. Drach ◽  
O. Bilyk ◽  
O. Matsjuk ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Immune System ◽  
Blood Serum ◽  
Antioxidant System ◽  
Total Protein ◽  
Lipid Hydroperoxides ◽  
Glutathione System ◽  
Endogenous Intoxication ◽  
Activity Of Enzymes ◽  
The Impact

The notion “endotoxin” is conditional, for any normal metabolit has a toxic impact of its excessive accumulation in organism. Endotoxins include products of breakdown of tissue proteins, peroxides and other products of free radical oxidation, low molecular weight toxins, toxins of microorganisms. Current knowledge on the mechanism of endotoxin action on the organism of pregnant cows is based on the key role of the immune system: it provides animals with protection against the infections, eliminates extraneous elements of endogenous and exogenous origin. It is defined that under endogenous intoxication, toxic metabolites suppress the activity of immune system of organism, decreases the resistability of an organism to negative effects of environmental factors. Animals under endogenous intoxication had the following clinical symptoms: swelling of mammal gland, edemas of external genitals, anemia of mucous membranes, functional disorders of proventriculuses and intestines, animals were stressed. It was defined that under endogenous intoxication in 8–9 months of pregnancy, blood of highly-productive cows had a tendency of decrease in the number of erythrocytes by 21.0%, in the level of hemoglobin – by 24.3%, increase in the number of leucocytes by 7.6% compared to the value for blood of cows with physiological pregnancy process. The development of endogenous intoxication among pregnant cows causes disorders in protein-synthesizing function of liver, which is indicated by decrease in the level of total protein and its fractions. It was defined that endogenous intoxication decreased the content of total protein in blood by 18.4%. Over the period of study, among pregnant cows under the development of endogenous intoxication, heightened activity of amino transferaces in the blood was observed. In the ninth month of pregnancy, the activity of amino transferaces in blood serum of cows from experimental group increased by 28.6%, and the activity of aspartate aminotransferace increased by 20.1% relatively. The activity of amino transferaces and decrease in the level of total protein and its fractions in blood serum is one of the first biochemical studies on the diagnosing endotoxin manifestations and indicates destructive processes in liver. It was defined that pregnant cows under the development of endogenous intoxication suffer disorders in detoxicating function of liver and disorders in filtrating function of kidneys. The developing endogenous intoxication of pregnant cows suppresses the activity of enzymes in their glutathione system of antioxidant protection, which is indicated by decrease in the activity of glutathione peroxidase by 27.5 %, glutathione reductase by 42.9%, glucose-6-phosphate dehydrogenase by 11.2% in blood serum. The lowest activity of enzymes of glutathione system in blood of highly-productive cows under endogenous intoxication was observed in their ninth month of pregnancy, which is related to increase in activation of processes of lipid peroxidation and misbalance between the activity of antioxidant system and intensity of lipid peroxidation. Reliable increase in the content of lipid hydroperoxides by 70.9% and in concentration of malondialdehide by 54.8% was observed. Misbalance towards generation of active forms of oxygen and their metabolites, exhaustion of antioxidant system and disorders in balance cause oxidative stress. 

scholarly journals EFFECT OF DIETARY ASPIRIN ON SOME BLOOD BIOCHEMICAL PARAMETERS OF BROILERS REARED IN HIGH DENSITY

2009 ◽  
Vol 33 (1) ◽  
pp. 38-43
Author(s):  
Motthena Mahmoud Shaker
Keyword(s):  
Uric Acid ◽  
Blood Serum ◽  
Total Protein ◽  
Biochemical Parameters ◽  
Serum Glucose ◽  
High Density ◽  
Cholesterol Concentration ◽  
Uric Acid Concentration ◽  
Blood Biochemical Parameters ◽  
Blood Biochemical

The objective of this study was to determine the effect of adding aspirin to the diet on somebiochemical parameters such as blood serum glucose , cholesterol , uric acid , total protein ,phosphorus and calcium concentrations of broilers reared in high density (15 birds / m ) . A 90 chicks 7days old were distributed into two treatments (45 chicks per treatment ) , T1 chicks fed a diet withoutaspirin (control) , chicks in T2 fed a diet supplemented with 0.2 % aspirin (2 mg aspirin / kg feed) .Each treatment subdivided into three replicates and each replicate reared in 1 X 1 m pen . The dataobtained revealed the following:Blood serum glucose concentration were 189 mg /100 ml , cholesterol concentration were 207mg /100 ml , uric acid concentration were 4.6 mg /100 mll of T1 chicks at 7 weeks of age , adding 0.2% of aspirin to the diet (T2) decreased blood serum glucose , cholesterol and uric acid of broiler chicksto 178 , 182 and 4.2 mg /100 ml respectively , in the same time adding aspirin to the diet increasedsignificantly (P<0.01) total protein concentration which were 5.1 gm /100 ml compaired with 4.6gm/100 ml of control chicks (T1) at 7 weeks of age . Adding aspirin to the diet increased significantly(P<0.01) blood serum phosphorus and calcium concentrations which indicated the importance ofadding aspirin in broiler diet to rebalance blood biochemical parameters and reducing crowding stressduring high density production

INFLUENCE OF THE GENOTYPE OF STEERS AND THE SEASON OF THE YEAR ON MORPHOLOGICAL AND BIOCHEMICAL PARAMETERS OF BLOOD

2021 ◽  
Vol 1 (1) ◽  
pp. 90-95
Keyword(s):  
Blood Serum ◽  
Red Blood Cells ◽  
Sea Level ◽  
Seasonal Dynamics ◽  
Total Protein ◽  
Blood Cells ◽  
Biochemical Parameters ◽  
External Environment ◽  
Adaptive Plasticity ◽  
Mountainous Area

The article analyzes the seasonal dynamics (winter, summer) of morphological and biochemical blood indicators characterizing the adaptive plasticity to the external environment of steers of locally improved livestock, Aberdeen-Angus, Kazakh white-headed and Kalmyk breeds, grown after weaning from mothers at the age of 8 months at an open feedlot of industrial type, located in a mountainous area within 1600 m above sea level. It was found that gobies of specialized meat breeds in all seasons of the year were characterized by greater blood saturation with red blood cells and hemoglobin and had an advantage over local improved cattle in terms of the content of total protein, albumins and globulins in blood serum.

scholarly journals DYNAMICS OF PROTEIN METABOLISM IN COWS WITH PROBIOTIC VETOM 1 BASED ON APATHOGENIC BACILLI

2019 ◽  
pp. 73-78
Author(s):  
G. A. Nozdrin ◽  
O. V. Lagoda ◽  
K. E. Surodina ◽  
A. G. Nozdrin ◽  
O. M. Gorshkova
Keyword(s):  
Blood Serum ◽  
Total Protein ◽  
Protein Metabolism ◽  
Biochemical Parameters ◽  
Control Group ◽  
Lactating Cows ◽  
Experimental Group

The researchers investigated the effect of new probiotic Vetom 1 based on apathogenic bacilli on the biochemical parameters of blood serum, exactly protein, albumins and urea in lactating cows. The experiment was carried out at OOO “Uchkhoz Tulinskoe”. According to the principle of pair-analogues, the researchers arranged a control group and two experimental groups of cows; each group contained 7 cows. The cows of the 1st experimental group were fed with probiotic Vetom 1 dosed 50 mg/kg once a day in the morning before milking; They received Vetom 1 every day during 5 days, then in a day during a month. The cows from the 2nd experimental group received probiotic Vetom 1 dosed 50 mg/kg once a day in the morning before milking, every day during 30 days. The application of the specimen contributed to a decrease in the concentration of total protein in the blood serum within the physiological norm. The aftereffect caused by Vetom 1 when the specimen was applied 5 days every day, then in a day during a month, the authors observed an increase in the concentration of total protein in the blood serum above the physiological standard on the 180 day of application. The similar effect was not observed when Vetom 1 was applied daily. When using Vetom 1, the authors observed a slight increase in the concentration of albumins in the blood serum, both during the period of application and up to 180 days. The specimen contributes to less prominent increase in the concentration of urea in the blood serum in comparison with the control group. Changes in concentrations of albumins and urea occurred within the physiological norm.

scholarly journals COMPREHENSIVE EVALUATION OF FATTENING AND MEAT QUALITIES OF YOUNG PIGS OF THE UNIVERSAL DIRECTION OF PRODUCTIVITY AND SOME OF THEIR INTERBIRELS FEATURES

2020 ◽  
Vol 21 (2) ◽  
pp. 205-212
Author(s):  
V. І. Khalak ◽  
O. S. Grabovska
Keyword(s):  
Blood Serum ◽  
Total Protein ◽  
Biochemical Parameters ◽  
Average Daily Gain ◽  
Live Weight ◽  
Thoracic Vertebrae ◽  
Comprehensive Index ◽  
Young Pigs ◽  
Fat Thickness ◽  
Daily Gain

The results of studies of the fattening and meat qualities of young pigs of large white breed of foreign origin, some biochemical parameters of blood serum and the results of calculating their correlation relationships are presented, and the economic efficiency of the research results is calculated. It was found that the biochemical parameters of blood serum in animals of the experimental group correspond to the physiological norm of clinically healthy animals, and their coefficient of variation ranges from 7.71 to 33.67 %. According to the main indicators of fattening and meat qualities (the age of reaching live weight of 100 kg, days, the thickness of the fat at the level of 6– 7 thoracic vertebrae, mm, the length of the chilled carcass, cm) corresponds to the “elite” class, and an effective assessment method sows and boars-producers by genotype is the use of a comprehensive index of feeding and meat qualities (B. Tyler index). The maximum indicators “average daily gain in live weight for the period of control fattening, kg”, “the length of the chilled carcass, cm”, “the length of the bacon half of the chilled carcass, cm” and the minimum values “age to reach live weight of 100 kg, days” and “bacon thickness the level of 6–7 thoracic vertebrae, mm ”is characterized by animals in which the comprehensive index of feeding and meat qualities (B. Tyler index) ranges from 160.14 to 174.04 points. Reliable correlation was established between the following pairs of signs: fat thickness at the level of 6–7 thoracic vertebrae, mm × total protein content, g / l (r=+ 0.375, tr=2.14), length of chilled carcass, cm × concentration of total lipoproteins , mg% (r=–0.622, tr=4.20), length of chilled carcass, cm × urea content, mmol/l (r=–0.445, tr=4.20), length of bacon half of chilled carcass, cm × content total protein, g / l (r=–0.533, tr=3.33), fat thickness at the level of 6–7 thoracic vertebrae, mm × concentration of total lipoproteins, mg% (r=–0.652, tr=4.55) the average daily gain in live weight for the period of control feeding, kg × concentration of total lipoproteins, mg% (r=+0.492, tr=2.99). The above indicates the prospect of using some biochemical indicators of blood serum for early prediction of fattening and meat qualities. It was established that the maximum increase in production in terms of "average daily gain in live weight during the period of control fattening, g" (4.87 %) was obtained from animals in which the B. Tyler index ranged from 160.14 to 174.04 points.

BIOCHEMICAL PARAMETERS OF BLOOD OF MONGREL RATS.

2020 ◽  
Vol 3 ◽  
pp. 163-168
Author(s):  
A. A. Stekolnikov ◽  
◽  
V. V. Reshetnyak ◽  
V. V. Burdeyny ◽  
E. A. Iskaliev ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
General Population ◽  
Blood Serum ◽  
Calculation Method ◽  
Total Protein ◽  
Biochemical Parameters ◽  
Total Bilirubin ◽  
Bile Pigment ◽  
Standard Methods ◽  
Experimental Animals

The aim of the work was to determine some biochemical parameters, taking into account the sex of the outbred (conventional) rats in the norm, used at the experiment to determine the reference val-ues. The experiments were carried out on 41 rats of 5-6 months age old., weighing 200-250 g, divided into two groups - males (n = 16) and fe-males (n = 25). Blood serum was obtained accord-ing to the generally accepted method. Blood serum was obtained according to standard methods. Biochemical parameters were analyzed on an IDEXX CatalystOne automated analyzer. Protein and carbohy-drate metabolism were analyzed by the se-rum levels of total protein (TP), albumin (Alb), urea (Urea), creatinine (Creat), glu-cose (Glu), the enzyme spectrum - by alka-line phosphatase (ALP), alanine and aspar-tate aminotransferase (ALT and AST), min-eral metabolism - by calcium (Ca), phospho-rus (P), sodium (Na), potassium (K), chlo-rine (Cl), as well as bile pigment - by total bilirubin (T.bil). De Ritis coefficient (AST / ALT), globulin (Glob), albumin/globulin ratio (A / G) was revealed by the calculation method. The results were taken into account in two versions: 1 - according to all animals (16 males and 25 females); 2 - according to samples from the general population in three replicates. It was found that out of 17 biochemical parameters -15 are constant, due to the sex of the experimental animals. So, females showed higher indicators of protein and min-eral metabolism, glucose, ALT, alkaline phosphatase, while in males this was record-ed only in four cases - in the content of urea and AST and in the ratios A / G, AST / ALT. Data on the content of creatinine and total bilirubin in the blood serum of rats were variable in nature and were close in value. Thus, the results of the studies carried out can be used in the future, for biochemical diagnostics of systematic metaboli

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