scholarly journals Biochemical changes in blood serum of rats with experimental esophageal burn II degree under treatment with aqueous extract of P. vulgaris pods

2017 ◽  
Vol 23 (2) ◽  
pp. 14-19
Author(s):  
V. Dmytryk ◽  
Ya. Raetska
Keyword(s):  
Phaseolus Vulgaris ◽  
Blood Serum ◽  
Therapeutic Effect ◽  
Aqueous Extract ◽  
Total Protein ◽  
Electrolyte Balance ◽  
Metabolic Systems ◽  
Immature Rats ◽  
Activity Of Enzymes ◽  
Esophageal Burn

During modeling of esophageal alkali burns II degree in immature rats were observed violations of various metabolic systems. It was shown that under the esophageal burns there occurred violations of protein (total protein, albumin), nitrogen metabolism (creatinine, urea), changed the activity of enzymes (GOT, GPT), violated fluid and electrolyte balance (Na+, К+, Cl-, Ca2+, Mg2+). In the groups of animals with the alkaline burn of the esophagus, that were injected aqueous extract of pod of bean (Phaseolus vulgaris) there can be traced a change of the indicators in the direction of normalization, indicating about the obvious therapeutic effect of aqueous extract of pod of bean (Phaseolus vulgaris).

scholarly journals Biochemical changes in blood serum of rats with experimental burn disease and their correction with melanin

2016 ◽  
Vol 21 (2) ◽  
pp. 44-48
Author(s):  
N. Chornenka ◽  
Ya. Rayetska ◽  
O. Savchuk ◽  
L. Ostapchenko
Keyword(s):  
Blood Serum ◽  
Total Protein ◽  
Biochemical Parameters ◽  
Electrolyte Balance ◽  
Experimental Burn ◽  
Metabolic Systems ◽  
Immature Rats ◽  
Urea Metabolism ◽  
Activity Of Enzymes ◽  
Burn Disease

During modeling of esophageal alkali burns 1 and 2 degrees in immature rats were observed violations of various metabolic systems, melanin administration helped to normalize the biochemical parameters. It was shown that under the esophageal burns there occurred violations of protein (total protein, albumin), nitrogen metabolism (creatinine, urea) metabolism of carbohydrate (glucose) and lipids (cholesterol), changed the activity of enzymes (GOT, GPT, CC), violated fluid and electrolyte balance (K +, Na +, Cl-, Ca2 +, Mg2 +, phosphates), while the introduction of melanin contributed to the normalization of these indicators. The most effective dose were of 0.5 mg/ kg and 1 mg/kg. There were found anti-toxic properties of melanin, which may be a promising drug for the normalization of metabolism in patients with burns of esophagus and prevent its complications.

scholarly journals The impact of endogenous intoxication on biochemical indicators of blood of cows with calves

2017 ◽  
Vol 8 (3) ◽  
pp. 438-443 ◽  
Author(s):  
B. Gutyj ◽  
Y. Grymak ◽  
M. Drach ◽  
O. Bilyk ◽  
O. Matsjuk ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Immune System ◽  
Blood Serum ◽  
Antioxidant System ◽  
Total Protein ◽  
Lipid Hydroperoxides ◽  
Glutathione System ◽  
Endogenous Intoxication ◽  
Activity Of Enzymes ◽  
The Impact

The notion “endotoxin” is conditional, for any normal metabolit has a toxic impact of its excessive accumulation in organism. Endotoxins include products of breakdown of tissue proteins, peroxides and other products of free radical oxidation, low molecular weight toxins, toxins of microorganisms. Current knowledge on the mechanism of endotoxin action on the organism of pregnant cows is based on the key role of the immune system: it provides animals with protection against the infections, eliminates extraneous elements of endogenous and exogenous origin. It is defined that under endogenous intoxication, toxic metabolites suppress the activity of immune system of organism, decreases the resistability of an organism to negative effects of environmental factors. Animals under endogenous intoxication had the following clinical symptoms: swelling of mammal gland, edemas of external genitals, anemia of mucous membranes, functional disorders of proventriculuses and intestines, animals were stressed. It was defined that under endogenous intoxication in 8–9 months of pregnancy, blood of highly-productive cows had a tendency of decrease in the number of erythrocytes by 21.0%, in the level of hemoglobin – by 24.3%, increase in the number of leucocytes by 7.6% compared to the value for blood of cows with physiological pregnancy process. The development of endogenous intoxication among pregnant cows causes disorders in protein-synthesizing function of liver, which is indicated by decrease in the level of total protein and its fractions. It was defined that endogenous intoxication decreased the content of total protein in blood by 18.4%. Over the period of study, among pregnant cows under the development of endogenous intoxication, heightened activity of amino transferaces in the blood was observed. In the ninth month of pregnancy, the activity of amino transferaces in blood serum of cows from experimental group increased by 28.6%, and the activity of aspartate aminotransferace increased by 20.1% relatively. The activity of amino transferaces and decrease in the level of total protein and its fractions in blood serum is one of the first biochemical studies on the diagnosing endotoxin manifestations and indicates destructive processes in liver. It was defined that pregnant cows under the development of endogenous intoxication suffer disorders in detoxicating function of liver and disorders in filtrating function of kidneys. The developing endogenous intoxication of pregnant cows suppresses the activity of enzymes in their glutathione system of antioxidant protection, which is indicated by decrease in the activity of glutathione peroxidase by 27.5 %, glutathione reductase by 42.9%, glucose-6-phosphate dehydrogenase by 11.2% in blood serum. The lowest activity of enzymes of glutathione system in blood of highly-productive cows under endogenous intoxication was observed in their ninth month of pregnancy, which is related to increase in activation of processes of lipid peroxidation and misbalance between the activity of antioxidant system and intensity of lipid peroxidation. Reliable increase in the content of lipid hydroperoxides by 70.9% and in concentration of malondialdehide by 54.8% was observed. Misbalance towards generation of active forms of oxygen and their metabolites, exhaustion of antioxidant system and disorders in balance cause oxidative stress. 

scholarly journals Mitigative effect of Momordica cymbalaria fruit extract against sodium fluoride induced hepatotoxicity in Wistar male albino rats

2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Raghavendra Mitta ◽  
Sushmitha Duddu ◽  
Raghuveer Yadav Pulala ◽  
Pradeepkumar Bhupalam ◽  
Venkatakirankumar Mandlem ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Blood Serum ◽  
Sodium Fluoride ◽  
Total Protein ◽  
Reduced Glutathione ◽  
Serum Biomarkers ◽  
Albino Rats ◽  
Serum Total Protein ◽  
Treatment Groups ◽  
Group I

AbstractObjectivesThe main objective of the present study is to evaluate the mitigative effect of hydroalcoholic extract of Momordica cymbalaria fruits against sodium fluoride (NaF) induced hepatotoxicity.MethodsIn this study, Wistar male albino rats were randomly divided into five groups of six rats each. Group I and II served as normal and toxic controls. Group III as plant control received extract at a dose of 400 mg/kg b. wt, p.o and Groups IV and V as treatment groups received extract at a dose 200 and 400 mg/kg b. wt, p.o for 30 days. All groups except Groups I and III received 100 ppm of NaF through drinking water. After completion of the study, blood collected for the estimation of liver blood serum biomarkers such as aspartate aminotransferases (AST), alanine aminotransferases (ALT), alkaline Phosphatase (ALP), direct and total bilirubin, total protein and albumin. The liver tissue homogenate was for estimation of lipid peroxidation, catalase, and reduced glutathione levels.ResultsThe results showed that NaF intoxication caused elevation of liver blood serum levels and lipid peroxidation; decreased levels of serum total protein, albumin and liver reduced glutathione, and catalase observed. The treatment groups showed decreased elevated serum biomarkers (ALT, AST, and ALP), liver lipid peroxidation and increased serum total protein and albumin, liver reduced glutathione and catalase levels in a dose-dependent manner. Histopathological studies also further strongly supported for mitigative effects of the plant.ConclusionsIn conclusion, our findings of the study indicated that M. cymbalaria fruits were a potential drug candidate in the treatment of NaF induced hepatotoxicity.

Fluorescence estimation of total protein in blood serum using N-doped carbon dots

2020 ◽  
Vol 33 ◽  
pp. 2233-2237
Author(s):  
R. Anjali ◽  
Abhishek Pathak ◽  
P.V. Suneesh ◽  
T.G. Satheesh Babu
Keyword(s):  
Blood Serum ◽  
Total Protein ◽  
Carbon Dots ◽  
Doped Carbon Dots

Aqueous extract of Ilex paraguariensis decreases nucleotide hydrolysis in rat blood serum

2005 ◽  
Vol 97 (1) ◽  
pp. 73-77 ◽  
Author(s):  
Milena Görgen ◽  
Kátia Turatti ◽  
Afonso R. Medeiros ◽  
Andréia Buffon ◽  
Carla D. Bonan ◽  
...  
Keyword(s):  
Blood Serum ◽  
Aqueous Extract ◽  
Ilex Paraguariensis ◽  
Nucleotide Hydrolysis ◽  
Rat Blood

scholarly journals Evaluation of hydrated extract of phaseolus Vulgaris L. (bean plant) on hypoglycemia and hypolipidemic in streptozotocin-induced diabetic albino Wistar rats

2019 ◽  
Vol 10 (4) ◽  
pp. 3704-3710
Author(s):  
Helisha Ruth Obonyo ◽  
Senthemarai Selvi V
Keyword(s):  
Phaseolus Vulgaris ◽  
Blood Serum ◽  
Common Bean ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Diabetic Rats ◽  
Low Density ◽  
Phaseolus Vulgaris L ◽  
Reference Drug ◽  
The Impact

The current research was intended to comprehend hypoglycemic and anti-lipidaemic exercises of hydrated common bean (phaseolus Vulgaris L.) seed extracts on streptozotocin-induced diabetic albino rats. At a set portion fluctuate of 100, 200,300 mg/kg body weight of common bean extracts was orally directed as one portion for every day to polygenic disorder rats for a measure of thirty days. The impact of P.vulgaris L. on hypoglycemic, glycosylated hemoprotein (HbA1c) and blood serum lipid profile (Total cholesterin), Triglyceride (TG), very low-density lipoprotein (VLDL), low-density lipoprotein (LDL), High-density lipoprotein (HDL)) in plasma were estimated in the regular and diabetic induced rat. The outcomes demonstrated that quick glucose,serum TC, TG, LDL, VLDL, levels were significantly (p<0.05) attenuate, while blood serum HDL, the level was extensively (p<0.05) upgraded inside the diabetic rats. The inconclusive amount of pace of 300 mg/kg is more reasonable than that of a hundred mg/kg. Our examination so shows that Phaseolus vulgaris L has a powerful adversary to diabetic and anti-lipidaemic impacts on streptozotocin-induced diabetic rats, and results were comparable to reference drug glibenclamide.

scholarly journals Therapeutic effect of aqueous extract of Globularia alypum following in vitro insulin induced toxicity on Rattus norvegicus cardiomyocytes

2017 ◽  
Vol 06 (01) ◽  
pp. 27-35
Author(s):  
Leila SMAIL ◽  
◽  
Sihem BERDJA ◽  
Saka BOUALEM ◽  
Samia NEGGAZI ◽  
...  
Keyword(s):  
Therapeutic Effect ◽  
Aqueous Extract ◽  
Rattus Norvegicus ◽  
Globularia Alypum

scholarly journals Variability of blood serum biochemical parameters in Karakachan sheep

2015 ◽  
Vol 31 (1) ◽  
pp. 55-62 ◽  
Author(s):  
O. Stevanovic ◽  
M. Stojiljkovic ◽  
D. Nedic ◽  
D. Radoja ◽  
V. Nikolic ◽  
...  
Keyword(s):  
Blood Serum ◽  
Total Protein ◽  
Age Groups ◽  
Balkan Peninsula ◽  
Inorganic Phosphorus ◽  
Sheep Breed ◽  
Mean Values ◽  
Indigenous Sheep ◽  
Significant Difference ◽  
Glutamyl Transferase

Karakachan sheep represents an endangered, indigenous sheep breed from Balkan Peninsula. There is relatively little information about the characteristics of this sheep breed in the veterinary scientific literature. The aim of this research was an examination of certain metabolic profile parameters of the Karakachan sheep blood serum, and variability of their concentrations in comparison to age and some other indigenous sheep breeds from Balkans. Examination was conducted on 14 clinically healthy sheep divided in two age groups. Blood samples were collected by puncture of v. jugularis and blood serum was separated after spontaneous coagulation. The concentrations of total protein, albumin, calcium, inorganic phosphorus, aspartate amino transferase (AST) and ?- glutamyl transferase (GGT) were determined. In relation to age of Karakachan sheep, statistically significant difference between the calculated mean values of examined parameters was not observed. A statistically significant difference was found between the mean concentrations of the studied parameters in Karakachan sheep and other breeds in total protein (Tsigai, Dubrovnik and Dalmatian sheep), albumin (Dalmatian), calcium and inorganic phosphorus (Tsigai, Dubrovnik) and AST's (Dalmatian, Karakachan sheep from Bulgaria).

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