scholarly journals Examination of Factors Influencing the Success Rates of an In Vitro Chemosensitivity Test for Lung Cancer Using Collagen Gel Droplet-Embedded Cultures

2019 ◽  
pp. 295-300
Author(s):  
Takahara Y ◽  
Kawasaki Y ◽  
Kato R ◽  
Shinomiya S ◽  
Oikawa T ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Drug Sensitivity ◽  
Sample Selection ◽  
Collagen Gel ◽  
Lower Percentage ◽  
Chemosensitivity Test ◽  
Success Rates ◽  
Cell Counts ◽  
Factors Influencing

Objective: We evaluated the success rates and factors influencing the success rates of the Collagen Gel Droplet-Embedded Culture Drug Sensitivity Test (CD-DST) for pleural effusate in lung cancer patients with carcinomatous pleurisy.Materials and methods: Thirty-two patients with lung cancer having carcinomatous pleurisy were enrolled in this study between January 2014 and May 2017. Their pleural effusions were analysed by CD-DST, and the subjects were classified into two groups: successful cases and unsuccessful cases, based on whether the test was able to successfully determine drug sensitivity. We investigated whether the properties of the pleural effusate might influence the success rate of CD-DST. We observed that the CD-DST tended to have good success rates in patients with higher pH, higher percentage of lymphocytes, higher cell counts, and lower percentage of neutrophils in their pleural effusate. The red blood cell count was higher in the effusate of unsuccessful than successful cases.Conclusion: Our results suggest that the diagnostic success rates of CD-DST for pleural effusion from patients with lung cancer might improve by appropriate sample selection.

RRM1 expression is verified as a biomarker predicting the drug sensitivity for GEM with in vitro 3D drug sensitivity test in non-small cell lung cancer tumor.

2017 ◽  
Vol 35 (15_suppl) ◽  
pp. e20054-e20054
Author(s):  
Nariyasu Nakashima ◽  
Dage Liu ◽  
Takayuki Nakano ◽  
Yusuke Kita ◽  
Xia Zhang ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Predictive Value ◽  
Drug Sensitivity ◽  
Collagen Gel ◽  
Small Cell ◽  
Cancer Drug ◽  
Small Cell Lung ◽  
Drug Sensitivity Test ◽  
Anti Cancer

e20054 Background: Ribonucleotide reductase M1 (RRM1) is involved in regulation of cell proliferation and synthesis of deoxyribonucleotides for DNA. It is also a cellular target for gemcitabine (GEM) and overexpression of RRM1 was reported to be associated with the resistance to GEM. Though RRM1 expression has been reported as the biomarkers in predicting the response to chemotherapy clinically, the value of GEM remains inconsistent and controversial. Collagen gel droplet embedded culture-drug sensitivity test (CD-DST) is a newly developed in vitro chemosensitivity test that could directly inspect the anti-cancer drug sensitivity with fresh tumor tissue. With use of CD-DST test, we have verified the predictive value of RRM1 expression to the anti-cancer agent sensitivity for GEM in non-small cell lung cancer (NSCLC) tumor. Here, the predictive value of biomarker RRM1 to GEM was further verified with CD-DST. Methods: Twenty-five patients with primary NSCLC were used in this study. Expression of RRM1 was assessed by immunohistochemistry. For CD-DST test, viable cells were collected from fresh surgical specimen and embed into the collagen gel droplets in 3D condition. Tumor cells were exposed to GEM for 1 hour and further incubated with serum-free culture medium for 7 days. The in vitro sensitivity was expressed as the percentage T/C ratio, where T was the total volume of the treated group and C was the total volume of the control group. Results: 1)Anti-cancer drug sensitivity: The sensitivity for GEM (T/C%) was 76.2 ± 30.5. 2)Expression of biomarkers: RRM1 expression was 39.2 ± 28.2 %. 3) Correlation: The expression of RRM1 significantly correlated with drug sensitivity for GEM (r = 0.446, p = 0.0256). Higher expression of RRM1 indicated worse anti-cancer drug sensitivity for GEM. Conclusions: The significant correlation between the RRM1 expression and sensitivity to GEM was proved with CD-DST in NSCLC tumors. The expression of RRM1 may become a useful biomarker in predicting the drug sensitivity for GEM in NSCLC.

O-45 Drug sensitivity test (in vitro growth assay) of gefitinib (Iressa, ZD1839) for primary lung cancer

Lung Cancer ◽  
2003 ◽  
Vol 41 ◽  
pp. S16
Author(s):  
Motoshi Takao ◽  
Kentaro Inoue ◽  
Fumiaki Watanabe ◽  
Tomohito Tarukawa ◽  
Masanori Kaneda ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Drug Sensitivity ◽  
Primary Lung Cancer ◽  
Sensitivity Test ◽  
In Vitro Growth ◽  
Drug Sensitivity Test ◽  
Growth Assay

In vitro-in vivo Correlation in Anticancer Drug Sensitivity Test Using AUC-Based Concentrations and Collagen Gel Droplet-Embedded Culture

Oncology ◽  
1996 ◽  
Vol 53 (3) ◽  
pp. 250-257 ◽  
Author(s):  
Makoto Inaba ◽  
Tazuko Tashiro ◽  
Shigeo Sato ◽  
Yasuyuki Ohnishi ◽  
Keizo Tanisaka ◽  
...  
Keyword(s):  
Anticancer Drug ◽  
Drug Sensitivity ◽  
Collagen Gel ◽  
Sensitivity Test ◽  
Drug Sensitivity Test

In vitro Chemosensitivity Test for Human Genitourinary Tumors Using Collagen Gel Matrix

1993 ◽  
Vol 24 (2) ◽  
pp. 267-270 ◽  
Author(s):  
T. Uchibayashi ◽  
M. Egawa ◽  
H. Hisazumi ◽  
T. Hasegawa ◽  
T. Tanaka ◽  
...  
Keyword(s):  
Collagen Gel ◽  
Chemosensitivity Test ◽  
In Vitro Chemosensitivity ◽  
In Vitro Chemosensitivity Test ◽  
Collagen Gel Matrix

Verification of biomarkers in predicting drug sensitivity with in vitro 3D drug sensitivity test in non-small cell lung cancer.

2016 ◽  
Vol 34 (15_suppl) ◽  
pp. 11602-11602
Author(s):  
Dage Liu ◽  
Nariyasu Nakashima ◽  
Yoshuke Kita ◽  
Yoshimasa Tokunaga ◽  
Kazuhito Nii ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Small Cell Lung Cancer ◽  
Cell Lung Cancer ◽  
Drug Sensitivity ◽  
Small Cell ◽  
Sensitivity Test ◽  
Small Cell Lung ◽  
Drug Sensitivity Test
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