scholarly journals Chitinase activity of biocontrol fungi cultured from the golden potato cyst nematode, Globodera rostochiensis

2020 ◽  
Vol 38 (1) ◽  
pp. 93-101
Author(s):  
Abbasi K. ◽  
Keyword(s):  
Chitinase Activity ◽  
Globodera Rostochiensis ◽  
Cyst Nematode ◽  
Potato Cyst Nematode ◽  
Biocontrol Fungi

Potato cyst nematode hatching activity and hatching factors in inter-specific Solanum hybrids

Nematology ◽  
2001 ◽  
Vol 3 (2) ◽  
pp. 141-149 ◽  
Author(s):  
Ken Devine ◽  
Peter Jones
Keyword(s):  
Solanum Tuberosum ◽  
Solanum Species ◽  
Globodera Rostochiensis ◽  
Cyst Nematode ◽  
Potato Cyst Nematode ◽  
Species Analysis ◽  
Specific Hybrid ◽  
Hybrid Clones ◽  
Different Levels

AbstractIn studies using three sibling F1 clones from each of five crosses between Solanum tuberosum and different wild tuberbearing Solanum species, root leachate hatching activity towards each of the two potato cyst nematode (PCN) species, Globodera rostochiensis and G. pallida, segregated independently. Sephadex G-10 fractionation of the leachates of S. sucrense-hybrid clones revealed differences between clones in the hatching factor elution profiles for the two PCN species. Analysis of individual hatching factors indicated examples both of selectivity (both PCN species hatched in response to a particular hatching factor, but at different levels of hatching response) and of specificity (only one PCN species hatched in response to a particular hatching factor) of hatching factors. The hatching factor profiles of the S. sucrense-hybrids indicated segregation of hatching factors from the parents, but also detected novel factors unique to specific hybrid clones. Total hatching activity of the root leachate of the S. sucrense clones was positively correlated with the proportion of S. tuberosum-derived hatching factors.

Identification of potato cyst nematode species Globodera rostochiensis and G. pallida by PCR using specific primer combinations

Nematology ◽  
1999 ◽  
Vol 1 (2) ◽  
pp. 157-163 ◽  
Author(s):  
Asier Fullaondo ◽  
Enrique Barrena ◽  
Miguel Viribay ◽  
Imanol Barrena ◽  
Azucena Salazar ◽  
...  
Keyword(s):  
Nematode Species ◽  
Globodera Rostochiensis ◽  
Cyst Nematode ◽  
Potato Cyst Nematode ◽  
Detection Methods ◽  
South American ◽  
Specific Primer ◽  
Pomme De Terre ◽  
Highly Sensitive ◽  
Specific Band

AbstractRAPD fragments specific for potato cyst nematode (PCN) species Globodera rostochiensis and G. pallida were identified. These were used to derive two primer combinations for PCR which amplify only one specific band in each species. The primer combinations were evaluated on 39 samples of PCN populations containing all currently described pathotypes as well as some South American PCN populations. This method for species identification is highly sensitive and also allows the detection of species mixtures in a sample. Furthermore, the generation of unique amplification products facilitates the application of automatic detection methods without electrophoretic separation and is therefore particularly useful for routine diagnoses. Identification des especes de nematodes a kyste de la pomme de terre Globodera rostochiensis et G. pallida par PCR grace a l'utilisation de combinaisons d'amorces specifiques - Des fragments RAPD specifiques des especes de nematodes a kyste de la pomme de terre (PCN) Globodera rostochiensis et G. pallida, ont ete identifies. A partir de ces fragments, deux combinaisons d'amorces, capables d'amplifier en PCR une seule bande, specifique pour chaque espece, ont ete obtenus. Les combinaisons d'amorces ont ete evaluees sur 39 echantillons de populations de PCN contenant tous les pathotypes decrits ainsi que quelques populations provenant d'Amerique du Sud. Cette methode d'identification d'espece est tres sensible et elle permet de plus l'identification d'un melange d'especes dans un meme echantillon. En sus, la generation de produits uniques d'amplification permet l'utilisation de methodes d'identification automatisees, sans separation electrophoretique, tres utiles donc pour le diagnostic de routine.

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