​Effect of Nutrients on Wilt in Chickpea

2021 ◽  
Author(s):  
B.D.S. Nathawat ◽  
O.P. Sharma ◽  
Manju Kumari ◽  
Hansraj Shivran
Keyword(s):  
Fusarium Oxysporum ◽  
Agricultural Research ◽  
Disease Incidence ◽  
Weather Conditions ◽  
Limiting Factors ◽  
Grain Legume ◽  
Nitrogen Levels ◽  
Chickpea Cultivar ◽  
Phosphorus Application ◽  
Wilt Incidence

Background: Chickpea (Cicer arietinum L.) contributes 18% of the global production of grain legume and serves as an important source of dietary protein. Fusarium wilt, caused by soil borne fungus Fusarium oxysporum f. sp. ciceri appears to be the most devastating diseases of chickpea throughout the world. It is one of the important limiting factors of chickpea production in India. The disease causes substantial yield losses which may reach even 100 per cent under favorable weather conditions. Methods: Pot culture experiments were conducted at Rajasthan Agricultural Research Institute, Durgapura, Jaipur during rabi 2014-15 and 2015-16 to study the effect of nutrients on wilt caused by Fusarium oxysporum f. sp. ciceri (Padwick) in chickpea (Cicer arirtinum). In the first experiment, the treatments comprised of four nitrogen levels-viz. 0, 15, 20 and 25 kg/ha and four phosphorus levels- viz. 0, 30, 40 and 50 kg/ha tried in sixteen treatment combinations. Surface sterilized seeds of chickpea cultivar L-550 were sown in each pot. After sowing, 400 ml water was supplied to each pot; there after watering was done at an interval of 6 days. In second experiment, the treatments comprised of 7 micronutrients viz. zinc, manganese, calcium, copper, cobalt, iron and nickel used at the rate of 10mg/kg of soil. Chickpea cultivar L-550 was used as a test crop. Result: Disease incidence was recorded periodically commencing 15 days after sowing and was continued up to 55 days after sowing. Levels of nitrogen application had increased wilt incidence successively in both the years. Whereas, a reverse trend was observed with the application of phosphorus, incidence of wilt was decreased with increasing levels of phosphorus. Application of zinc and calcium reduced the wilt incidence significantly over check. Zinc was found to be most effective in minimizing the disease incidence.

scholarly journals Evaluation of Green Manure Amendments for the Management of Fusarium Basal Rot (Fusarium oxysporumf.sp.cepae) on Shallot

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
Assefa Sintayehu ◽  
Seid Ahmed ◽  
Chemeda Fininsa ◽  
P. K. Sakhuja
Keyword(s):  
Green Manure ◽  
Agricultural Research ◽  
Disease Incidence ◽  
Limiting Factors ◽  
Lepidium Sativum ◽  
Ethiopian Mustard ◽  
Basal Rot ◽  
Fusarium Basal Rot ◽  
Manure Amendments

Shallot (Allium cepaL. var.ascalonicum) is the most traditional vegetable crop in Ethiopia. Shallot is susceptible to a number of diseases that reduce yield and quality, among which fusarium basal rot (FBR) caused byFusarium oxysporumf.sp.cepae (Foc)is one of the most important yield limiting factors in Ethiopia. The present study was designed to evaluate the effectiveness ofBrassicacrops for the management of shallot FBR on shallot. The experiments were carried out at Debre Zeit Agricultural Research Center using cabbage (Brassica oleracea), garden cress (Lepidium sativum), Ethiopia mustard (B. carinata), and rapeseed (B. napus). The evaluations were done underin vitroand greenhouse conditions. Underin vitrotest condition it was confirmed that extracts of Ethiopian mustard and rapeseed showed higher inhibition on the growth ofFocpathogen compared to control. Data on seedling emergence, plant height, plant stand, disease incidence, severity, cull bulbs, and bulb weight were collected in greenhouse experiment. The green manure amendments of rapeseed and Ethiopian mustard significantly reduced disease incidence by 21% and 30% and disease severity by 23% and 29%, respectively. However the plant emergency was not significantly different among treatments in greenhouse test. These results indicated that Ethiopian mustard and rapeseed crops have potential as green manure for the management of FBR disease of shallot crop.

scholarly journals Efficacy of fungicides in controlling fusarium wilt of gladiolus

2018 ◽  
Vol 42 (4) ◽  
pp. 599-607
Author(s):  
L Yasmin ◽  
MA Ali ◽  
FN Khan
Keyword(s):  
Fusarium Oxysporum ◽  
Fusarium Wilt ◽  
Field Condition ◽  
Agricultural Research ◽  
Disease Incidence ◽  
Wilt Disease ◽  
Spike Length ◽  
Research Centre ◽  
Agricultural Research Institute ◽  
Infested Field

The efficacy of fungicides in controlling Fusarium wilt of gladiolus was studied at Horticulture Research Centre (HRC), Bangladesh Agricultural Research Institute (BARI), Gazipur during 2010-2012 following RCB design with four replications. Six fungicides such as Bavistin (0.1%), Provax (0.2%), Mancozeb (0.2%), Rovral (0.2%), Chlorax (10%) and Cupravit (0.7%) were evaluated against the Fusarium wilt disease of gladiolus (Fusarium oxysporum f. sp. gladioli) under naturally infested field condition. Bavistin was very effective in reducing the disease incidence and thereby resulting maximum corm germination (99.98%), spike length (73.90 cm), rachis length (43.70 cm), florets spike-1 (12.63), flower sticks plot-1 (38.75) and corm plot-1 (60.23) and cormel yield ha-1 (2.51 t) of gladiolus. Provax and Cupravit were also effective in inhibiting the disease incidence as well as better spike length, rachis length, florets spike-1, no of flower sticks, corm and cormel yield.Bangladesh J. Agril. Res. 42(4): 599-607, December 2017

scholarly journals SURVEY ON THE INCIDENCE OF FUSARIUM WILT OF TOMATO INCITED BY FUSARIUM OXYSPORUM F.SP. LYCOPERSICI (FOL) IN MAJOR TOMATO GROWING AREAS OF KRISHNAGIRI DISTRICT

2020 ◽  
Vol 21 (supplemenet 1) ◽  
Author(s):  
K. Vignesh ◽  
K. Rajamohan ◽  
P. Balabaskar ◽  
R. Anandan ◽  
R. Udhayakumar
Keyword(s):  
Fusarium Oxysporum ◽  
Fusarium Wilt ◽  
Fungal Pathogen ◽  
Disease Incidence ◽  
Morphological Characteristics ◽  
Wilt Disease ◽  
Tomato Seedlings ◽  
Collar Region ◽  
Wide Range ◽  
Wilt Incidence

Tomato is one of the most important, commercial and widely grown vegetable crop in the world. It is affected by several fungal, bacterial and viral diseases. Among these Fusarium wilt caused by the fungus Fusarium oxysporum f.sp. lycopersici causes 30-40% yield loss. A survey was conducted to investigate the incidence and severity of Fusarium wilt incited by Fusarium oxysporum f.sp. lycopersici in ten major tomato growing areas of Krishnagiri district. The occurrence of wilt disease incidence ranged from 18 % to 49% was noticed. Plant showing typical symptoms were taken from 10 fields and identified based on symptom appearance as well as morphological characteristics. The result of the survey revealed that wide range of infection and severity of wilt disease were occurred in the major tomato growing areas in Krishnagiri district. Isolation of the pathogen associated with tomato wilt was made from the diseased tissues in roots and collar region of the plant on the Potato dextrose agar (PDA) medium. Fol3 recorded the maximum wilt incidence followed by Fol4 and the minimum wilt incidence was recorded by Fol6 . The pathogenicity of the fungal pathogen was also proved after artificial inoculation of the tomato seedlings

scholarly journals A New Report of Cymbidium spp. Pseudobulb Rot Orchestrated by Erwinia carotovora, Fusarium oxysporum, and Mucor hiemalis f. sp. hiemalis

Plant Disease ◽  
2006 ◽  
Vol 90 (11) ◽  
pp. 1460-1460
Author(s):  
S. Sen ◽  
R. Acharya ◽  
A. Saha ◽  
K. Acharya
Keyword(s):  
Fusarium Oxysporum ◽  
Agricultural Research ◽  
Disease Incidence ◽  
Erwinia Carotovora ◽  
Eastern Himalaya ◽  
Distilled Water ◽  
Synergistic Activity ◽  
Iowa State College ◽  
Mucor Hiemalis ◽  
Epidemic Disease

Cymbidium spp. is an orchid of great horticultural value cultivated extensively in Eastern Himalaya, India. Since 1995, growers have experienced huge crop losses in every monsoon month because of pseudobulb rot. Pseudobulbs initially turned soft and pulpy followed by oozing of a dark brown liquid with a foul odor (early phase). With increasing severity, the bulbs and roots lose weight as the internal tissues gradually disintegrate (middle phase). Finally, the bulb becomes hollow, fibrous, and dry causing death of the plant (later phase). Surveys from 2002 to 2005 showed that disease incidence ranged from 60 to 100%. Rotted tissue was plated on nutrient agar and potato dextrose agar media. Three organisms were consistently isolated from 50 samples collected from 30 different localities. They were identified as Erwinia carotovora (2), Fusarium oxysporum (3), and Mucor hiemalis f. sp. hiemalis (1) and were predominant at the earlier, middle, and later stages of disease, respectively. Identifications were further confirmed by the Agricultural Research Institute (ARI), Pune, India. Pseudobulbs were surface sterilized with 0.5% sodium hypochlorite for 1 min, washed by sterile distilled water, and dipped separately into three different spore/cell suspensions (105 CFU/ml) for 1 min. Another set of sterilized bulbs was dipped first into E. carotovora, then into F. oxysporum 12 days later, and then into M. hiemalis f. sp. hiemalis 15 days after the second dip. For the control set, bulbs were dipped into sterile distilled water. Samples were incubated aseptically at 20°C with a relative humidity of 80%, and all inoculated bulbs were evaluated for disease 47 days after the first inoculation. When samples were inoculated separately, E. carotovora exhibited maximum (70%) tissue disintegration followed by F. oxysporum (30%) and M. hiemalis f. sp. hiemalis (10%), but none of the individual pathogens caused 100% tissue disintegration. Complete destruction was observed after 47 days of first inoculation when these three pathogens were inoculated consecutively according to their serial occurrence. It is an interesting report on host-pathogen combination as three pathogens act in sequence toward ultimate demolition of the host. We report this rot as a synergistic activity of three pathogens to cause an uncontrolled epidemic disease of Cymbidium spp. References: (1) J. C. Gilman. Page 37 in: A Manual of Soil Fungi. Iowa State College Press. Ames, IA, 1945. (2) J. G. Holt. Page 469 in: Bergey's Manual of Systematic Bacteriology. Vol. I. Williams and Wilkins. Baltimore/London, 1984, (3) C. V. Subramaniam. Page 657 in: Hyphomycetes. Indian Council of Agricultural Research (ICAR). New Delhi, 1971.

scholarly journals Evaluation of Chickpea Varieties and Fungicides for the Management of Chickpea Fusarium Wilt Disease (Fusarium oxysporum f.sp. ciceris) at Adet Sick Plot in Northwest Ethiopia

2018 ◽  
Vol 2018 ◽  
pp. 1-7
Author(s):  
Yigrem Mengist ◽  
Samuel Sahile ◽  
Assefa Sintayehu ◽  
Sanjay Singh
Keyword(s):  
Grain Yield ◽  
Fusarium Oxysporum ◽  
Fusarium Wilt ◽  
Agricultural Research ◽  
Disease Incidence ◽  
Wilt Disease ◽  
Disease Progress Curve ◽  
Disease Progress ◽  
Fusarium Wilt Disease ◽  
Progress Curve

A 2-year experiment was conducted at wilt sick plot infested with natural occurring Fusarium oxysporum f.sp. ciceris at Adet Agricultural Research Center in northwestern Ethiopia with an aim to evaluate effective chickpea varieties and fungicides for the management of chickpea fusarium wilt in order to integrate chickpea varieties and fungicides. Four varieties, namely, Shasho, Arerti, Marye, and local, two fungicides, namely, Apron Star and mancozeb, and untreated local chickpea were used as treatments. Treatments were arranged in a factorial combination in randomized complete block design in three replications. There were significant differences at p<0.05 in the overall mean of fusarium wilt disease incidence, area under disease progress curve %-day, yield and yield components among varieties and fungicides treatments. Data were analyzed using SAS system version 9.2. The results indicated that the maximum disease incidence and area under disease progress curve values 65.62% and 578.5%-day, respectively, were recorded from untreated local chickpea, while the minimum disease incidence and area under disease progress curve values 23.41% and 147%-day, respectively, were recorded from Shasho variety treated with Apron Star. The maximum biomass and grain yield of 6.71 t/ha and 4.6 t/ha, respectively, were recorded from Shasho variety treated with Apron Star while the minimum biomass and grain yield of 0.62 t/ha and 0.21 t/ha, respectively, were recorded from untreated local chickpea. Thus, the experiment results suggested that the variety of Shasho treated with fungicide Apron Star caused significant reduction in chickpea fusarium wilt incidence leading to a corresponding increase in grain yield of chickpea.

Nutrient economy through land configuration and residue management in a greengram (Phaseolus radiatus L.)-wheat (Triticum aestivum L.) cropping sequence with limited water supplies

2005 ◽  
Vol 52 (4) ◽  
pp. 369-379
Author(s):  
B. G. Shivakumar ◽  
B. N. Mishra ◽  
R. C. Gautam
Keyword(s):  
Grain Yield ◽  
Agricultural Research ◽  
Indian Agricultural Research Institute ◽  
Residual Effect ◽  
Residue Management ◽  
Phosphorus Fertilization ◽  
Significant Interaction Effect ◽  
Preceding Crop ◽  
Cropping Sequence ◽  
Phosphorus Application

A field experiment on a greengram-wheat cropping sequence was carried out under limited water supply conditions in 1997-98 and 1998-99 at the farm of the Indian Agricultural Research Institute, New Delhi. The greengram was sown either on flat beds or on broad beds 2 m in width, divided by furrows, with 0, 30 and 60 kg P2O5/ha. After the harvest of greengram pods, wheat was grown in the same plots, either with the greengram stover removed or with the stover incorporated along with 0, 40, 80 and 120 kg N/ha applied to wheat. The grain yield of greengram was higher when sown on broad beds with furrows compared to flat bed sowing, and the application of 30 or 60 kg P2O5/ha resulted in significantly higher grain yields compared to no phosphorus application. The combination of broad bed and furrows with phosphorus fertilization was found to be ideal for achieving higher productivity in greengram. The land configuration treatments had no impact on the productivity of wheat. The application of phosphorus to the preceding crop had a significant residual effect on the grain yield of wheat. The incorporation of greengram stover also significantly increased the grain yield of wheat. The increasing levels of N increased the grain yield of wheat significantly up to 80 kg/ha. The combination of greengram stover incorporation and 80 kg N/ha applied to wheat significantly increased the grain yield. Further, there was a significant interaction effect between the phosphorus applied to the preceding crop and N levels given to wheat on the grain yield of wheat.

Assessment of reproductive capacity of representatives of ancestral complexes and especially their selection of seed in dry conditions

2019 ◽  
pp. 1-23
Author(s):  
А.В. Семенютина ◽  
С.Е. Лазарев ◽  
К.А. Мельник
Keyword(s):  
Weather Conditions ◽  
Reproductive Capacity ◽  
Limiting Factors ◽  
Scientific Center ◽  
Gleditsia Triacanthos ◽  
Biological Potential ◽  
Forest Reclamation ◽  
Dry Conditions ◽  
Generative Phase ◽  
Selection Of

Представители родовых комплексов Robinia (Робиния), Gleditsia (Гледичия) относятся к экономически важным и перспективным, для деградированных ландшафтов засушливого региона, видам растений. Несмотря на это до последнего времени отсутствуют сведения по многим видам этих родовых комплексов, связанные с вопросами экологических основ семеноведения и их репродуктивной способности в условиях светлокаштановых почв Волгоградской области. Все это направлено на выявление и расширение перспективных ареалов культивирования для лесомелиорации и озеленения населенных пунктов. Цель исследований изучение репродуктивной способности представителей родовых комплексов Robinia (Робиния), Gleditsia (Гледичия) в коллекциях ФНЦ агроэкологии РАН и выявление особенностей их селекционного семеноведения для лесомелиорации и озеленения населенных пунктов в сухостепных условиях. Объектами исследований являлись виды и формы рода Robinia: R. viscosa Vent. R. neomexicana Gray. R. pseudoacacia L. R. neomexicana х pseudoacacia, и Gleditsia (G. triacanthos L., G. triacanthos L. f. inermis, G. caspica Desf, G. texana Sarg., G. aquatica, G. japonica, G. caspica, G. sinensis), произрастающие в кластерных участках коллекций ФНЦ агроэкологии РАН, кадастр 34:34:000000:122, 34:34:060061:10. Разработка основ селекционного семеноведения базируется на изучении репродуктивных особенностей выделенных для целевого использования собственных биоресурсов с учетом возможностей всестороннего изучения генеративных качеств и оценки биологического потенциала. Выявлено, что стабильность плодоношения фиксируется через несколько лет после вступления в генеративную фазу: у Robinia и Gleditsia 67 лет. Первое цветение у Gleditsia triacanthos и G. texana, G. aquatica наблюдалось в возрасте 5 лет, G. japonica 6, G. caspica и G. sinensis 8 лет. Завязываемость плодов зависит не только от возраста растения, но и от погодных условий во время цветения. На семенную продуктивность влияет количество выпавших осадков и сумма активных температур в период созревания плодов. Формирование более крупных плодов и семян наблюдается в возрасте до 15 лет. В результате исследований (20172019 гг.) выявлено влияние лимитирующих факторов на биологический потенциал цветения, плодоношения и семенную продуктивность для определения ареалов их культивирования. Representatives of generic systems Robinia, Gleditsia are economically important and promising for degraded arid landscapes of the region, types of plants. Despite this, until recently, there is no information on many types of these generic complexes related to the environmental foundations of seed and their reproductive capacity in light chestnut soils of the Volgograd region. All this is aimed at identifying and expanding promising areas of cultivation for forest reclamation and greening of settlements. The aim of the research is to study the reproductive capacity of representatives of ancestral complexes Robinia, Gleditsia in the collections of the Federal scientific center for Agroecology Russian Academy of Sciences and the identifying features of their selection of seed for forest reclamation and landscaping of settlements in the dry steppe conditions. The objects of research were species and forms of the genus Robinia: R. viscosa Vent. R. neomexicana Gray. R. pseudoacacia L. R. neomexicana x pseudoacacia and Gleditsia (G. triacanthos L., G. triacanthos L. f. inermis, G. caspica Desf, G. texana Sarg., G. aquatica, G. japonica, G. caspica, G. sinensis), growing in the cluster areas of collections of FSC Agroecology RAS, cadastre 34:34:000000:122, 34:34:060061:10. The development of the principles of seed breeding is based on the study of reproductive characteristics of the allocated for the targeted use of their own bioresources, taking into account the possibilities of a comprehensive study of generative qualities and assessment of biological potential. It was found that the stability of fruiting is fixed a few years after entering the generative phase: Robinia and Gleditsia 67 years. The first flowering in Gleditsia triacanthos and G. texana, G. aquatica was observed at the age of 5 years, G. japonica 6, G. caspica and G. sinensis 8 years. Fruit setability depends not only on the age of the plant, but also on weather conditions during flowering. Seed productivity is affected by the amount of precipitation and the amount of active temperatures during the ripening period. The formation of larger fruits and seeds is observed in the age of 15 years. As a result of studies (20172019), the influence of limiting factors on the biological potential of flowering, fruiting and seed production to determine the areas of their cultivation was revealed.

Biological control of Fusarium wilt of cucumber with nonpathogenic isolates of Fusarium oxysporum

1987 ◽  
Vol 33 (5) ◽  
pp. 349-353 ◽  
Author(s):  
T. C. Paulitz ◽  
C. S. Park ◽  
R. Baker
Keyword(s):  
Biological Control ◽  
Fusarium Oxysporum ◽  
Infection Rate ◽  
Fusarium Wilt ◽  
Disease Incidence ◽  
Infection Rates ◽  
Control Activity ◽  
Significant Difference ◽  
Cucumber Roots ◽  
Linear Regressions

Nonpathogenic isolates of Fusarium oxysporum were obtained from surface-disinfested, symptomless cucumber roots grown in two raw (nonautoclaved) soils. These isolates were screened for pathogenicity and biological control activity against Fusarium wilt of cucumber in raw soil infested with Fusarium oxysporum f. sp. cucumerinum (F.o.c.). The influence of three isolates effective in inducing suppressiveness and three ineffective isolates on disease incidence over time was tested. The effective isolates reduced the infection rate (R), based on linear regressions of data transformed to loge (1/1 – y). Effective isolate C5 was added to raw soil infested with various inoculum densities of F.o.c. In treatments without C5, the increase in inoculum densities of F.o.c. decreased the incubation period of wilt disease, but there was no significant difference in infection rate among the inoculum density treatments. Isolate C5 reduced the infection rate at all inoculum densities of F.o.c. Various inoculum densities of C5 were added to raw soils infested with 1000 cfu/g of F.o.c. In the first trial, infection rates were reduced only in the treatment with 10 000 cfu/g of C5; in the second trial, infection rates were reduced in treatments with 10 000 and 30 000 cfu/g of C5.

scholarly journals Marker Development for Differentiation of Fusarium oxysporum f. sp. Niveum Race 3 from Races 1 and 2

2021 ◽  
Vol 22 (2) ◽  
pp. 822
Author(s):  
Owen Hudson ◽  
Sumyya Waliullah ◽  
James C. Fulton ◽  
Pingsheng Ji ◽  
Nicholas S. Dufault ◽  
...  
Keyword(s):  
South Carolina ◽  
Fusarium Oxysporum ◽  
Fusarium Wilt ◽  
Conventional Polymerase Chain Reaction ◽  
Detection Methods ◽  
Limiting Factors ◽  
Pcr Marker ◽  
Primer Sets ◽  
Race Differentiation ◽  
Race 2

Fusarium wilt of watermelon, caused by Fusarium oxysporum f. sp. niveum (FON), is pathogenic only to watermelon and has become one of the main limiting factors in watermelon production internationally. Detection methods for this pathogen are limited, with few published molecular assays available to differentiate FON from other formae speciales of F. oxysporum. FON has four known races that vary in virulence but are difficult and costly to differentiate using traditional inoculation methods and only race 2 can be differentiated molecularly. In this study, genomic and chromosomal comparisons facilitated the development of a conventional polymerase chain reaction (PCR) assay that could differentiate race 3 from races 1 and 2, and by using two other published PCR markers in unison with the new marker, the three races could be differentiated. The new PCR marker, FNR3-F/FNR3-R, amplified a 511 bp region on the “pathogenicity chromosome” of the FON genome that is absent in race 3. FNR3-F/FNR3-R detected genomic DNA down to 2.0 pg/µL. This marker, along with two previously published FON markers, was successfully applied to test over 160 pathogenic FON isolates from Florida, Georgia, and South Carolina. Together, these three FON primer sets worked well for differentiating races 1, 2, and 3 of FON. For each marker, a greater proportion (60 to 90%) of molecular results agreed with the traditional bioassay method of race differentiation compared to those that did not. The new PCR marker should be useful to differentiate FON races and improve Fusarium wilt research.

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