scholarly journals ​Binasoybean-6: A High Yielding Mutant Soybean Variety Developed through Sustainable Mutation Breeding

2022 ◽  
Author(s):  
M.A. Malek ◽  
R.M. Emon ◽  
M.K. Khatun ◽  
M.S.H. Bhuiyan ◽  
Adedze Yawo Mawunyo Nevame ◽  
...  
Keyword(s):  
Gamma Rays ◽  
Agronomic Traits ◽  
Insect Pests ◽  
Mutation Breeding ◽  
Superior Performance ◽  
Food Protein ◽  
Soybean Variety ◽  
Ecological Zones ◽  
Commercial Cultivation ◽  
Mutant Lines

Background: Soybean is an important source of food, protein and oil and hence more research is essential to increase its yield under different agro-ecological conditions, including stress. In this regard, four popular soybean varieties viz. Shohag, BDS-4, BAU-S/64 and BARI Soybean-5 were irradiated using Co60 gamma rays to create genetic variation for earliness, higher seed yield and other desirable agronomic traits. Methods: The experiments were conducted at Bangladesh Institute of Nuclear Agriculture (BINA) Headquarters farm, Mymensingh during 2006-2009 and 28 elite mutant lines were selected for evaluation. The mutant line, SBM-22 derived from mother variety BARI Soybean-5 irradiated with 300Gy of gamma rays was found to be superior compared to other mutants. Considering the superior performance of mutant SBM-22 including 28 mutants and mother check variety BARI Soybean-5, were evaluated through different trials. The evaluation trials were conducted at different agro-ecological zones of the country during Rabi season (January to April) of 2010-2018. Result: Significant variations were observed both in individual location and over locations for all traits. Reactions to major diseases and insect-pests infestation were also studied. Due to better performance of the mutant SBM-22, Bangladesh Institute of Nuclear Agriculture (BINA) applied to the National Seed Board (NSB) of Bangladesh for registration as an important soybean variety “Binasoybean-6”. Consequently, the NSB of Bangladesh registered SBM-22 as an improved soybean variety in 2019 as Binasoybean-6 for commercial cultivation.

scholarly journals Studies on Induced Physical and Chemical Mutagenesis in Groundnut (Arachis hypogia)

2015 ◽  
Vol 35 ◽  
pp. 25-35 ◽  
Author(s):  
A. Gunasekaran ◽  
P. Pavadai
Keyword(s):  
Gamma Rays ◽  
Seedling Survival ◽  
Dry Weight ◽  
Mutation Breeding ◽  
Chemical Mutagenesis ◽  
Program Selection ◽  
Methane Sulphonate ◽  
Ld50 Value ◽  
Mutant Lines ◽  
Physical And Chemical

Mutation breeding has been widely used for the improvement of plant characters in various crops. It is a powerful and effective tool in the hands of plant breeders. In any mutation breeding program, selection of an effective and efficient mutagen is very essential to produce high frequency of desirable mutation. Groundnut (Arachis hypogia) var. VRI-2. was treated with different concentration of physical and chemical mutagen namely gamma rays 10, 20, 30, 40, 50 and 60 KR and Ethyl methane sulphonate (EMS). For inducing mutation various concentration of EMS such as 0.1, 0.2, 0.3, 0.4, 0.5 and 0.6 % for six hours were applied to 200 seed sample of each concentration and one respective control. The LD50 value was observed in 50% of gamma rays and 0.5 % of EMS. The morphological and yield characters were significantly reduced seed germination, seedling survival, days to first flower, plant height, number of leaves per plant, number of grains per plant, grain length and breath, 100 grains weight, grain yield per plant, fresh and dry weight per plant. The increasing doses/concentration of gamma rays and EMS decreased in phenotypic and yield characters in M1generation. The mutagenized populations showed significantly higher variability in the M2 generation. Mutant lines showing higher yield per plant than the respective parents and checks were isolated in M2 and subsequent generation were significantly more pod yield and yield components than the untreated plants.

Development of the first kabuli type chickpea mutant variety in Bangladesh.

2021 ◽  
pp. 203-208
Author(s):  
Shamsun Nahar Begum ◽  
Mirza Mofazzal Islam ◽  
Rigyan Gupta
Keyword(s):  
Management Practices ◽  
Insect Pest ◽  
Grey Mould ◽  
Coat Colour ◽  
Mutation Breeding ◽  
High Yield ◽  
Commercial Cultivation ◽  
High Yielding Varieties ◽  
Successive Generations ◽  
Mutant Lines

Abstract Chickpea has a high yield potential, nutritional importance and diversity of use. A mutation breeding programme was undertaken at Bangladesh Institute of Nuclear Agriculture (BINA) with a view to developing early-maturing, large-seeded and high-yielding varieties of chickpea. Seeds of the popular chickpea variety 'Desi Binasola-2' were treated with different doses of gamma-rays (200, 300 and 400 Gy). The treated seeds were grown in batches according to dose for raising the M1 generation. M2 seeds were collected from individual M1 plants and subsequently grown in plant-progeny rows in the M2 generation and selections were made from the M2 families. Only 85 plants were selected from the M2 population and these were grown in the M3 generation. The mutant 'CPM-kabuli' and 28 other mutants were selected from M3 and were grown in the M4 generation. Only five mutants, including 'CPM-kabuli', were selected from M4 and were grown in M5. The selected mutant 'CPM-kabuli' along with check varieties were put into preliminary yield trials. Finally, the mutant lines were evaluated, with respect to two check varieties, in advanced, zonal-yield, on-farm and on-station trials in successive generations. All the selected mutant lines were grown at different locations in Bangladesh to observe the yield and other characteristics. The performance of the mutants was evaluated under two management practices: research management and farmers' management. Contrary to its parent, 'CPM-kabuli' was found to be tolerant to root rot and Botrytis grey mould, and also showed greater tolerance to pod borer insect-pest infestation than other mutants and check varieties. The main improved attributes are a cream seed coat colour, which reflects kabuli type, larger seed size and higher seed yield. The mutant 'CPM-kabuli' matures in the range of 115-125 days and is high yielding (1.7 t/ha). Considering all these, the bold Kabuli type chickpea mutant 'CPM-kabuli' was registered as the variety 'Binasola-9' for commercial cultivation during 2017 and is suitable for farmers in drought-prone areas in Bangladesh.

Mutagenic Effectiveness and Efficiency of Gamma Rays and HZ with Phenotyping of Induced Mutations in Lentil Cultivars

2017 ◽  
Vol 64 ◽  
pp. 17-31 ◽  
Author(s):  
Rafiul Amin Laskar ◽  
Samiullah Khan
Keyword(s):  
Gamma Rays ◽  
Agronomic Traits ◽  
Mutation Breeding ◽  
Induced Mutations ◽  
Morphological Variations ◽  
Combination Treatments ◽  
Base Parameters ◽  
Wide Range ◽  
Effectiveness And Efficiency ◽  
Growth Habits

In mutation breeding, mutagenic effectiveness and efficiency are the base parameters to predict the mutagenic potency of any mutagen. Studies on mutagenic effectiveness and mutagenic efficiency of physical mutagen (gamma rays) and chemical mutagen (hydrazine hydrates; HZ) on two cultivars of lentil (Lens culinaris Medik.), viz. DPL 62 (macrosperma) and Pant L 406 (microsperma) have been reported. Dry and healthy seeds were treated with four doses of each gamma rays (100-400 Gy), HZ (0.1-0.4 %) and their combinations. Frequencies of the induced agro-morphological variations into different phenotypic categories were estimated in M2 population that resulted into identification and isolation of wide range of mutants with altered phenotypes. Data on effectiveness and efficiency of various mutagenic treatments calculated on the basis of the frequency of chlorophyll mutations, which showed effectiveness and efficiency were higher at the moderate doses of gamma rays and HZ, while in case of combination treatments; lower doses were most effective and efficient with few inter-varietal exceptions. Phenotyping of the mutants revealed that growth habits was the most sensitive category to which most of the mutant belongs, followed by leaf and flower/pod/seed in both the cultivars studied. Overall, the screened and isolated mutants with economically important agronomic traits can be further propagated in the subsequent generation for development of elite lentil mutant cultivars.

scholarly journals Construction of Soybean Mutant Diversity Pool (MDP) Lines and an Analysis of Their Genetic Relationships and Associations Using TRAP Markers

Agronomy ◽  
2020 ◽  
Vol 10 (2) ◽  
pp. 253 ◽  
Author(s):  
Dong-Gun Kim ◽  
Jae Il Lyu ◽  
Min-Kyu Lee ◽  
Jung Min Kim ◽  
Nguyen Ngoc Hung ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Linear Model ◽  
Agronomic Traits ◽  
Genetic Relationships ◽  
Mixed Linear Model ◽  
Mutation Breeding ◽  
Population Variation ◽  
Phenotypic Traits ◽  
Single Factor Analysis ◽  
Mutant Lines

Mutation breeding is useful for improving agronomic characteristics of various crops. In this study, we conducted a genetic diversity and association analysis of soybean mutants to assess elite mutant lines. On the basis of phenotypic traits, we chose 208 soybean mutants as a mutant diversity pool (MDP). We then investigated the genetic diversity and inter-relationships of these MDP lines using target region amplification polymorphism (TRAP) markers. Among the different TRAP primer combinations, polymorphism levels and polymorphism information content (PIC) values averaged 59.71% and 0.15, respectively. Dendrogram and population structure analyses divided the MDP lines into four major groups. According to an analysis of molecular variance (AMOVA), the percentage of inter-population variation among mutants was 11.320 (20.6%), whereas mutant intra-population variation ranged from 0.231 (0.4%) to 14.324 (26.1%). Overall, intra-population genetic similarity was higher than that of inter-populations. In an analysis of the association between TRAP markers and agronomic traits using three different statistical approaches based on the single factor analysis (SFA), the Q general linear model (GLM), and the mixed linear model (Q+K MLM), we detected six significant marker–trait associations involving five phenotypic traits. Our results suggest that the MDP has great potential for soybean genetic resources and that TRAP markers are useful for the selection of soybean mutants for soybean mutation breeding.

Mutation breeding in six rowed barley: Screening for stem rust resistance in certain induced mutants

2021 ◽  
Vol 27 (2) ◽  
Author(s):  
Shipra Raj
Keyword(s):  
Gamma Rays ◽  
Stem Rust ◽  
Mutation Breeding ◽  
Loss Assessment ◽  
Resistant Lines ◽  
Induced Mutants ◽  
Biological Yield ◽  
Induced Mutagenesis ◽  
Mutant Lines ◽  
Moderately Resistant

During the induced mutagenesis in six rowed barley, 18 mutant lines were isolated from the M2 and M3 generations. Thirteen lines were induced by gamma rays, four by EMS and one by HA. These lines were screened for resistance to stem rust and loss assessment. Mutants were grown in field as well as in earthen pots. Artificial inoculums were provided in condition suitable for rust occurrence. Rust reaction was recorded according to modified Cabb’s scale and the rust intensity was classified as 0,5, 10, 25, 40 and 65 percent. Lines K50-38, K50-97, K50-102 and K40-73 were completely free from infection (0 %) and thought to be immune lines(I). K50-47, KE-4-47 and G50-47 exhibited traces of symptoms and placed under 5% intensity and were very resistant(R). Line KE2-63 exhibited 10% intensity and classified as moderately resistant(MR). Line K50-44; K40-11, K40-67, K30-91, KE3-75, G50-35 and GH3-32 showed 25% intensity of pustules and were put into moderately susceptible(MS) class. One line K30-82 showed 40% intensity and classified as susceptible(S), while line KE4-87 exihibited 65% intensity and was classified as heavily susceptible. Class 100 percent was not observed. Immune, very resistant and moderately resistant were identified as resistant lines and selected for further study, while moderately susceptible and heavily susceptible lines were identified as susceptible lines and discarded. Reduction in yield contributing traits i.e. number of grains/spike, test weight, seed yield and biological yield was also observed which was positively correlated with disease intensity.

Increased yield performance of mutation induced Soybean genotypes at varied agro-ecological conditions

2024 ◽  
Vol 84 ◽  
Author(s):  
M. S. H. Bhuiyan ◽  
M. A. Malek ◽  
R. M. Emon ◽  
M. K. Khatun ◽  
Mohammad Moneruzzaman Khandaker ◽  
...  
Keyword(s):  
Seed Yield ◽  
Large Scale ◽  
Gamma Ray ◽  
Mutation Breeding ◽  
Continuous Selection ◽  
Parent Material ◽  
Soybean Variety ◽  
Branch Number ◽  
Yield Performance ◽  
Ecological Zones

Abstract In soybean breeding program, continuous selection pressure on traits response to yield created a genetic bottleneck for improvements of soybean through hybridization breeding technique. Therefore an initiative was taken to developed high yielding soybean variety applying mutation breeding techniques at Plant Breeding Division, Bangladesh Institute of Nuclear Agriculture (BINA), Bangladesh. Locally available popular cultivar BARI Soybean-5 was used as a parent material and subjected to five different doses of Gamma ray using Co60. In respect to seed yield and yield attributing characters, twelve true breed mutants were selected from M4 generation. High values of heritability and genetic advance with high genotypic coefficient of variance (GCV) for plant height, branch number and pod number were considered as favorable attributes for soybean improvement that ensure expected yield. The mutant SBM-18 obtained from 250Gy provided stable yield performance at diversified environments. It provided maximum seed yield of 3056 kg ha-1 with highest number of pods plant-1 (56). The National Seed Board of Bangladesh (NSB) eventually approved SBM-18 and registered it as a new soybean variety named ‘Binasoybean-5’ for large-scale planting because of its superior stability in various agro-ecological zones and consistent yield performance.

Lethal Dose (LD50) Fixation and Sensitivity of Fenugreek (Trigonellafoenum-graecumL.) to Gamma Radiation for Induction of Mutation

2017 ◽  
Vol 4 (04) ◽  
Author(s):  
ANURADHA PATEL ◽  
POONAM VERMA ◽  
SHARDA CHOUDHARY ◽  
ARVIND KUMAR VERMA
Keyword(s):  
Genetic Variability ◽  
Gamma Rays ◽  
Growth Parameters ◽  
Seedling Survival ◽  
Lethal Dose ◽  
Germination Percentage ◽  
Mutation Breeding ◽  
Vigour Index ◽  
Small Flower ◽  
Dose Dependent

Fenugreek (Trigonella foenum-graecumL.) is an annual crop, mainly used as a spiceand leafy vegetable crop in many parts of the world. Classical breeding in fenugreek is restricted due to its low genetic variability and small flower size which hamper manual emasculation and pollination. Mutation breeding is an effective way to enrich genetic variability in crop plants. An experiment was conducted to determine the lethal dose of the physical mutagen gamma rays in fenugreek. The dry seeds of fenugreek were exposed to different doses of gamma rays i.e. 150Gy, 200Gy, 250Gy, 300Gy and 350Gy. These irradiated seeds were sown in the Petri plates with non-irradiated seeds (control). As the dose of gamma rays increased, there was a decrease in germination percentage, seedling survival, root length, shoot length and vigour index. Among five doses of gamma rays, the maximum seed germination was observed at lowest dose 150Gy (93%), followed by 200Gy (83%), 250Gy (76%), 300Gy (76%) and 350Gy (64%). The seedling survival was decreased from 90% (in control) to 56% in 350Gy dose of gamma rays. The gamma rays dose of 150Gy gave stimulatory effect on seedlings growth. The growth parameters were dose dependent, as the dose of gamma rays increased from 200Gy to 350Gy. The gamma rays dose of 350Gy showed 64% seeds germination and 56% of seedlings survival. Therefore, it is concluded that the LD50 dose for fenugreek is close to 350Gy. This information would be highly useful for initiating mutation breeding programme in fenugreek

scholarly journals Comparative Experimental Effects of Intercropping and Cypermethrin on Insect Pest Infestation and Yield of Maize, Cowpea and Okra in Two Cameroonian Agro-Ecological Zones

2021 ◽  
Vol 3 (2) ◽  
pp. 383-393
Author(s):  
Patient Farsia Djidjonri ◽  
Nukenine Elias Nchiwan ◽  
Hartmut Koehler
Keyword(s):  
Field Experiments ◽  
Insect Pests ◽  
Cropping Systems ◽  
Insect Pest ◽  
Maize Yield ◽  
Ecological Zones ◽  
Pest Infestation ◽  
Final Yield ◽  
Cropping Seasons ◽  
Agro Ecological Zones

The present study investigates the effect of intercropping (maize-cowpea, maize-okra, maize-okra-cowpea, okra-cowpea) compared to insecticide application on the level of infestation of insect pests and the final yield of maize, cowpea and okra. Field experiments were conducted during the 2016 and 2017 cropping seasons in the Guinean Savannah (Dang-Ngaoundere) and Sudano Sahelian (Gouna-Garoua) agro-ecological zones in Cameroon. Our experimental design was a split plot arrangement in a randomized complete block with four replications. The main factor was assigned to the use of insecticide (Cypermethrin) and sub plots were devoted for cropping systems. We compared the efficiency of intercropping to that of Cypermethrin application on the Yield of maize, cowpea and okra as influenced by insect pest damages. The comparison of monocropped sprayed by Cypermethrin to unsprayed showed that, in Dang, insect pests reduced maize yield by 37% and 24% in 2016 and 2017, respectively, whereas in Gouna, it was lower than 8% during the both years. Reduction in seed yield by insect pests on cowpea in Dang represented 47% and 50% in 2016 and 2017, respectively, whereas in Gouna, it was 55% and 63% in 2016 and 2017, respectively. For okra, insect pests reduced okra fruit yield by 25% and 44% in Dang and 23% and 28% in Gouna, respectively, in 2016 and 2017. Crop yield was lower in intercropping compared to monoculture due to competition of plants in association on different resources. Considering the total yields obtained from each intercropping, intercropping trials resulted generally in higher yields compared to mono-culture (LER > 1) in both sites and years but the respective yields were quite different. On the basis of the results obtained, we recommend maize-cowpea intercropping as a sustainable solution to reduce the infestation level of their pest insects.

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