Protective role of aqueous extract of Phyllanthus amarus on oxidative stress in pancreas of streptozotocin induced diabetic male Wistar rats

2016 ◽  
Vol 2 (1) ◽  
pp. 23 ◽  
Author(s):  
Sasi Bhusana Rao Bongu ◽  
Saisree Sagree ◽  
Vijayabharathi Gudapareddy ◽  
Malliah Putakala ◽  
Sreenivasulu Nukala ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Aqueous Extract ◽  
Reduced Glutathione ◽  
Protective Role ◽  
Pancreatic Tissue ◽  
Diabetic Rats ◽  
Phyllanthus Amarus ◽  
Glutathione S Transferase ◽  
Male Wistar Rats

Diabetes mellitus has been associated with oxidative stress. The present study was performed to investigate the pancreas protective activities of Phyllanthus amarus aqueous extract (PAAEt) in streptozotocin (STZ) induced diabetic rats. Oral administration of PAAEt (200mg/kg bw) to diabetic rats for 60 days significantly (p<0.05) decreased antioxidant enzymes such as glutathione peroxidase, glutathione-s-transferase, glutathione reductase, superoxide dismutase, catalase and depletion of reduced glutathione in panceas which illustrates that increased lipid peroxidation (107 %) was restored to control levels. Co-treatment with PAAEt revealed the therapeutic efficacy against oxidative stress in pancreatic tissue. Hence extract of this plant could be used as an adjuvant therapy for the prevention and/or management of diabetes.

Influence of flavonoid extracts from celery on oxidative stress induced by dichlorvos in rats

2011 ◽  
Vol 31 (6) ◽  
pp. 617-625 ◽  
Author(s):  
J Cao ◽  
X Zhang ◽  
Q Wang ◽  
L Jia ◽  
Y Zhang ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Treated Group ◽  
Protective Role ◽  
Normal Diet ◽  
Intragastric Administration ◽  
Glutathione S Transferase ◽  
Male Wistar Rats ◽  
Mda Content ◽  
Gpx Activity

The present work was to investigate the effects of flavonoid extracts from celery on oxidative stress induced by dichlorvos (DIC) in male Wistar rats maintained on a normal diet. The rats were given DIC through intragastric administration by the dose of 7.2 mg/kg·body weight (bw)/day and additionally added 5% flavonoid extracts to the diet for 4 weeks continuously. The activities of superoxide dismutase (SOD), glutathione peroxidase (GPx), catalase (CAT), glutathione- S-transferase (GST) and the content of malondialdehyde (MDA) in livers of rats were measured at the end of the experiment. Under the influence of DIC, there were significant decrease in the activities of SOD, CAT and GST and significant increase in GPx activity and MDA content. The results also showed that the activities of SOD, GST and CAT in the DIC-treated group declined significantly when compared with the flavonoid extracts group and the DIC + flavonoid extracts group, respectively. With regard to GPx activity and MDA content, significant increase were showed in the DIC-treated group in comparison to those in the flavonoid extracts group and the DIC + flavonoid extracts group, respectively. The observations presented lead us to conclude the harmful effects of DIC during the exposure and the protective role of flavonoids in minimizing these effects.

Protective role of Sterculia tragacantha aqueous extract on pancreatic gene expression and oxidative stress parameters in streptozotocin-induced diabetic rats

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Basiru Olaitan Ajiboye ◽  
Babatunji Emmanuel Oyinloye ◽  
Jennifer Chidera Awurum ◽  
Sunday Amos Onikanni ◽  
Adedotun Adefolalu ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Body Weight ◽  
Protective Role ◽  
Diabetic Rats ◽  
Gene Expressions ◽  
Ki 67 ◽  
Oxidative Stress Parameters ◽  
And Oxidative Stress ◽  
Stress Parameters

Abstract Objectives The current study evaluates the protective role of aqueous extract of Sterculia tragacantha leaf (AESTL) on pancreatic gene expressions (insulin, PCNA, PDX-1, KI-67 and GLP-1R) and oxidative stress parameters in streptozotocin-induced diabetic rats. Methods Diabetes mellitus was induced into the experimental Wistar animals via intraperitoneal (IP) injection of streptozotocin (35 mg/kg body weight) and 5% glucose water was given to the rats for 24 h after induction. The animals were categorized into five groups of 10 rats each as follows normal control, diabetic control, diabetic rats administered AESTL (150 and 300 mg/kg body weight) and diabetic rats administered metformin (200 mg/kg) orally for two weeks. Thereafter, the animals were euthanized, blood sample collected, pancreas harvested and some pancreatic gene expressions (such as insulin, PCNA, PDX-1, KI-67, and GLP-1R)s as well as oxidative stress parameters were analyzed. Results The results revealed that AESTL significantly (p<0.05) reduced fasting blood glucose level, food and water intake, and lipid peroxidation in diabetic rats. Diabetic rats administered different doses of AESTL showed a substantial upsurge in body weight, antioxidant enzyme activities, and pancreatic gene expressions (insulin, PCNA, PDX-1, KI-67, and GLP-1R). Conclusions It can therefore be concluded that AESTL has the ability to protect the pancreas during diabetes mellitus conditions.

scholarly journals Protective Effect of Quercetin on Melphalan-Induced Oxidative Stress and Impaired Renal and Hepatic Functions in Rat

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Ebenezer Tunde Olayinka ◽  
Ayokanmi Ore ◽  
Olaniyi Solomon Ola ◽  
Oluwatobi Adewumi Adeyemo
Keyword(s):  
Anticancer Agents ◽  
Hepatic Function ◽  
Protective Role ◽  
Glutathione S Transferase ◽  
Drug Induced ◽  
Treatment Groups ◽  
Plasma Bilirubin ◽  
Male Wistar Rats ◽  
Mda Content

One major challenge with the use of anticancer agents is the phenomenon of drug-induced toxicity. Melphalan (MPLN) is an alkylating anticancer agent, while quercetin (QCT) is an antioxidant. We investigated the protective role of quercetin against MPLN-induced toxicity. Twenty-five male Wistar rats (160–170 g) were randomized into five treatment groups; (I) control, (II) MPLN (0.2 mg/kg b.w.), (III) pre-treated with QCT (20 mg/kg b.w.) for 7 days followed by MPLN (0.2 mg/kg b.w.) for 7 days, (IV) cotreated with QCT (20 mg/kg b.w.) and MPLN (0.2 mg/kg b.w.) for 7 days, and (V) QCT (20 mg/kg b.w.) alone. MPLN caused a significant increase in plasma bilirubin, urea, and creatinine by 122.2%, 102.3%, and 188%, respectively (P<0.05). Similarly, plasma ALP, ALT, AST, and γ-GT activities increased significantly by 57.9%, 144.3%, 71.3%, and 307.2%, respectively, relative to control. However, pre or cotreatment with QCT ameliorated the levels of renal and hepatic function indices. Hepatic ascorbic acid and GSH and activities of glutathione-S-transferase, SOD, and catalase decreased significantly by 36.2%, 188%, 46.5%, 34.4%, and 55.2%, respectively, followed by increase in MDA content by 46.5% relative to control. Pre- and cotreatment with QCT reestablished the hepatic antioxidant status and lipid peroxidation. Overall, quercetin protected against MPLN-induced renal and hepatic toxicity in rats.

scholarly journals Antioxidant and hepatoprotective property of squalene for counteracting the oxidative damage induced by methotrex- ate in experimental rats

2021 ◽  
Vol 64 (2) ◽  
pp. 199-206
Author(s):  
Edakkukaran Sudhakaran Sumi ◽  
Pavan Kumar Dara ◽  
Rosemol Jacob Mannuthy ◽  
Balaraman Ganesan ◽  
Rangasamy Anandan ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Reduced Glutathione ◽  
Histopathological Examination ◽  
Lipid Peroxide ◽  
Mucosal Injury ◽  
Protective Role ◽  
Bone Marrow Toxicity ◽  
Shark Liver Oil ◽  
Limited Application

Methotrexate (MTX), an antifolate drug, is extensively prescribed for patients suffering from diseases like cancer, psoriasis, neoplasms, and rheumatoid arthritis. Despite its effectiveness, MTX sometimes finds limited application because its undesirable side effects, including hepatic or renal impairment, bone marrow toxicity and gastrointestinal mucosal injury. Squalene, a highly unsaturated isoprenoid compound, isolated from shark liver oil has great potential in neutralizing the damaging effects triggered by free radicals. Therefore, in this study, the protective role of dietary squalene supplementation on oxidative stress induced by methotrexate in experimental rats was evaluated. A significant reduction was displayed in the activities of catalase (CAT) and superoxide dismutase (SOD) in MTX-intoxicated groups compared to other groups. Similarly, the activities of glutathione dependant enzymes (GPx and GST) and reduced glutathione (GSH) in MTX-induced groups were shown to be lower compared to the untreated control. Increased LPO (lipid peroxide) level was found in MTX-intoxicated groups compared to other groups. In addition, alterations in the levels of liver marker enzymes like AST, ALP, ALT, and LDH were noticed in MTX intoxicated groups compared to other groups. Biochemical results were confirmed by the histopathological examination of liver sections. In conclusion, the result obtained in the present study proposes that squalene exerts antioxidant activity and is capable of ameliorating oxidative stress and liver injury induced by MTX.

scholarly journals The protective role of virgin coconut oil on the alloxan-induced oxidative stress in the liver, kidneys and heart of diabetic rats

2019 ◽  
Vol 10 (4) ◽  
pp. 2114-2124 ◽  
Author(s):  
Siniša Đurašević ◽  
Nebojša Jasnić ◽  
Marko Prokić ◽  
Ilijana Grigorov ◽  
Vesna Martinović ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Coconut Oil ◽  
Protective Role ◽  
Diabetic Rats ◽  
Toxic Effects ◽  
Virgin Coconut Oil ◽  
Tissue Specific

Our results show that VCO supplementation ameliorates some toxic effects of alloxan induced diabetes, and that the changes are tissue specific.

scholarly journals Cellular Apoptosis, Mitochondrial Swelling, Permeability and Cytochrome-C Level After (Fe3o4)-Nps Nanoparticles Exposure and Protective Role of Diferuloylmethane in Rats Liver

2020 ◽  
Vol 7 (1) ◽  
pp. 140-154
Author(s):  
Zina Bouteraa ◽  
Rachid Rouabhi ◽  
Fouad Menaceur ◽  
Salim Gasmi
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Protective Role ◽  
Mitochondrial Swelling ◽  
Male Rats ◽  
Glutathione S Transferase ◽  
Defensive Role ◽  
Cellular Apoptosis ◽  
Cyt C

AbstractDuring recent years the defensive role of diferuloylmethane against oxidative stress and apoptosis has been experimentally documented. Fe3O4-NPs can cause cellular death by inducing oxidative stress. Present study aimed to investigate whether diferuloylmethane could protect rats mitochondria against Fe3O4-NPs intoxication. Twenty adult male rats were randomly chosen and divided into four groups: control; treated with 10 mg/kg/d of Fe3O4-NPs; treated with diferuloylmethane at the dose 20 ml/kg/d; treated with Fe3O4-NPs (10 mg/kg/d) and diferuloylmethane (20 ml/kg/d) respectively for 28 days. The results showed that Fe3O4-NPs increased the Alanine aminotransferase (ALT), aspartate aminotransferase (AST), lipid peroxidation, mit-GSH (Glutathione), mit-CAT (Catalase), mit-GST (Glutathione S-transferase) and decreased mit-GPx (Glutathione peroxidase), with increased in mitochondrial swelling and permeability followed by the increasing level of plasmatic Cyt-c. The addition of diferuloylmethane (DFM) to these samples reduces or corrects the amount of the most of biomarkers. These findings have demonstrated that DFM can act as an antioxidant and antiapoptotic factor against damages induced by Fe3O4-NPs.

scholarly journals Liver Dysfunction and Oxidative Stress in Streptozotocin-Induced Diabetic Rats: Protective Role of Artemisia Turanica

2019 ◽  
Vol 22 (2) ◽  
pp. 109-114
Author(s):  
Hassan Bgheri Yazdi ◽  
Vida Hojati ◽  
Abdolhossein Shiravi ◽  
Sara Hosseinian ◽  
Gholamhassan Vaezi ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Liver Dysfunction ◽  
Protective Role ◽  
Diabetic Rats ◽  
And Oxidative Stress

Possible prenatal impact of sertraline on human placental glutathione S-transferase-π

2011 ◽  
Vol 31 (5) ◽  
pp. 457-464 ◽  
Author(s):  
O Dalmizrak ◽  
G Kulaksiz-Erkmen ◽  
N Ozer
Keyword(s):  
Congenital Malformations ◽  
Inhibitory Concentration ◽  
Reduced Glutathione ◽  
Protective Role ◽  
Tricyclic Antidepressant ◽  
Glutathione S Transferase ◽  
Natural Ligand ◽  
The Brain

Sertraline (SER), a tricyclic antidepressant, is considered to belong to the group of selective amine reuptake inhibitors. Its ability to cross the blood–brain barrier and transplacental transport has been reported previously. It is widely distributed in the brain and is bound to human glutathione S-transferase-π (GST-π). If SER is taken during pregnancy, it gets accumulated in the embryo and fetus, and some studies have suggested it may cause congenital malformations, thus the study of the interaction of GST-π with antidepressants is crucial. In this study, the interaction of human placental GST-π with SER in the presence of the natural ligand, reduced glutathione (GSH) and a xenobiotic ligand, 1-chloro-2,4-dinitrobenzene (CDNB) was investigated. The Vmvalues obtained at variable [CDNB] and variable [GSH] were 61.3 ± 2.3 and 46.4 ± 1.7 U/mg protein, respectively. The kcatand kcat/ Kmvalues for GSH and CDNB were 3.63 × 106s−1, 2.59 × 1010M−1s−1and 4.79 × 106s−1, 1.29 × 1010M−1s−1, respectively. The half maximal inhibitory concentration value for SER was 4.60 mM. At constant [CDNB] and variable [GSH] the inhibition type was linear mixed-type, with Ks, α, and Kivalues of 0.14 ± 0.02, 2.90 ± 1.64, and 2.18 ± 0.80 mM, respectively. On the other hand, at fixed [GSH] and at variable [CDNB], the inhibition type was competitive, with Kivalue of 0.96 ± 0.10 mM. Thus, these findings weaken the importance of the protective role of GST against toxic electrophiles in vivo in adults, but due to its immature enterohepatic system SER may accumulate in the fetus and cause congenital malformations.

scholarly journals Protective Effect of Diosgenin on Streptozotocin Induced Diabetic Nephropathy in Experimental Rats

2020 ◽  
pp. 201-207
Author(s):  
Pankaj G. Jain ◽  
Dipali J. Patil ◽  
Priti G. Nayase ◽  
Sanjay J. Surana ◽  
Pramod P. Patil
Keyword(s):  
Oxidative Stress ◽  
Plasma Glucose ◽  
Reduced Glutathione ◽  
Glycosylated Hemoglobin ◽  
Kidney Injury ◽  
Vascular Diseases ◽  
Protective Role ◽  
Diabetic Rats ◽  
Steroidal Saponin ◽  
Dependent Manner

Diabetes mellitus is a multifactorial metabolic disorder associated with the diabetes related vascular diseases. Oxidative stress along with inflammation is the Key factor leading to diabetic complications. Present study was designed to investigate the protective role of diosgenin, a steroidal saponin, in diabetes induced early kidney injury and oxidative stress markers and histopathological changes in kidney of diabetic rats, induced by single intra-peritoneal injection of streptozotocin (55 mg/kg weight (b.w.). After 72 hrs, experimental rats received diosgenin at different doses (10, 20 and 40 mg/kg b.w.) once daily for four weeks. At the end of the experiment, diabetic rats showed a significant increase in the levels of plasma glucose, glycosylated hemoglobin with a significant decrease in insulin and total hemoglobin. The activities of antioxidant enzymes such as superoxide dismutase, catalase, reduced glutathione, and the levels of reduced glutathione were decreased while increases in the levels of lipid peroxidation markers were observed in kidney tissues of diabetic rats. Oral administration of diosgenin to diabetic rats considerably shrivelled the plasma glucose and exaggerated the endocrine level supported a dose dependent manner. Diosgenin at a dose of 40 mg/kg b.w. was more pronounced effect than the other two doses and used for further studies. All the manifestations observed in diabetic rats were significantly reversed to near normal at a dose of 40 mg/kg b.w. of diosgenin. These findings recommend that diosgenin may have a helpful role against excretory organ harm evoked by aerobic stress within the diabetic state.

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