scholarly journals Fatty acid biosynthesis and transcriptional regulation of Stearoyl-CoA Desaturase 1 (SCD1) in Buffalo milk

2020 ◽  
Author(s):  
Zhipeng Li ◽  
Suyu Lu ◽  
Kuiqing Cui ◽  
Laiba Shafique ◽  
Saif ur Rehman ◽  
...  
Keyword(s):  
Transcriptional Regulation ◽  
Fatty Acid ◽  
Epithelial Cells ◽  
Fatty Acid Biosynthesis ◽  
Mammary Epithelial Cells ◽  
Buffalo Milk ◽  
Point Of View ◽  
Altered Expression ◽  
Expression Of Genes ◽  
Stearoyl Coa Desaturase

Abstract Background: Buffalo milk is considered as a highly nutritious food owing to its higher contents of fatty acids (FA)and rich nutrient profile. Higher fat contents of buffalo milk make it suitable for processing to develop various healthy and nutritious products. Moreover, buffalo milk contains more unsaturated FAs (UFA) such as oleic and linolenic acid, which are important from the human health point of view owing to their desirable physiological effects. However, inadequate information is available about the chemical composition and mechanism of FA synthesis in buffalo milk. In this study, we hypothesized that expression of SCD 1 gene could alter the biosynthesis of FA in epithelial cells of mammary gland and subsequently affect the FA contents in b uffalo milk. We investigated the transcriptional and biological role of Stearoyl-CoA Desaturase 1 ( SCD 1) in the buffalo mammary epithelial cells (BMECs) during FA and triacylglycerol (TAG) synthesis. Results : Results revealed that unsaturated fatty acid contents were much higher in concentration in buffalo milk as compared to Holstein cow. Significant increase in the expression level of FAS , ACACA , SREBP 1, PPARG , GPAT , and AGPAT genes was observed in response to altered expression of SCD 1 in buffalo milk . Moreover, change in SCD1 gene in BMECs also mediated the expression of genes related to FA biosynthesis subsequently leading to alter the FA composition. Overexpression of SCD1 significantly increased the expression of genes associated with FA and TAG synthesis leading to enhance FA and unsaturated FA contents in BMECs. However, down-regulation of SCD1 exhibited opposite consequences. Conclusion: Our study provides mechanistic insights on transcriptional regulation of SCD 1 to alter FA and TAG synthesis through directly or indirectly mediating biosynthesis and metabolic pathways in BMECs. We provide preliminary findings regarding engineering of FA contents in buffalo milk through SCD 1 signaling.

scholarly journals Fatty acid biosynthesis and transcriptional regulation of Stearoyl-CoA Desaturase 1 (SCD1) in Buffalo milk

2019 ◽  
Author(s):  
Zhipeng Li ◽  
Suyu Lu ◽  
Kuiqing Cui ◽  
Laiba Shafique ◽  
Saif ur Rehman ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Epithelial Cells ◽  
Nutritive Value ◽  
Fatty Acid Biosynthesis ◽  
Mammary Epithelial Cells ◽  
Buffalo Milk ◽  
Mammary Epithelial ◽  
Food Component ◽  
Expression Of Genes ◽  
Stearoyl Coa Desaturase

Abstract Background: Nutritionally, buffalo milk is of immense importance due to high contents of fatty acid (FA), highly energetic and nutritive value. The composition of FA has been regarded as functional food component, which facilitate to manufacture a variety of processed milk products. Moreover, Buffalo milk contains more unsaturated FAs (UFA) such as oleic and linolenic acids, which have been shown to exert a number of physiological effects. However, inadequate information reported about the chemical composition and mechanism of FA synthesis in buffalo milk. Our conducted research hypothesize that, the expression of SCD1 gene could alter the biosynthesis of fatty acid (FA) in epithelial cells of mammary gland and subsequently affect the FA contents in buffalo milk. Present study investigated transcriptional and biological role of Stearoyl-CoA Desaturase 1 (SCD1) in the buffalo mammary epithelial cells (BMECs) during FA and triacylglycerol (TAG) synthesis. Results: Results showed that unsaturated fatty acid contents are much higher in concentration in buffalo milk as compared to Holstein cow. Significantly, higher expression level of FAS, ACACA, SREBP1, PPARG, GPAT and AGPAT genes were also been observed with the expression of the SCD1 in buffalo milk. Moreover, studies showed that, SCD1 gene alteration in BMECs also causes the change in the expression of FA synthesis related genes mentioned above and subsequently affect the FA composition in the cells. Overexpression of SCD1 significantly increased the expression of genes associated with FA and TAG synthesis and also improved the FA and unsaturated FA contents in BMECs, while down-regulation of SCD1 appear opposite consequences. Conclusion: Thus, present study clearly demonstrated that, alteration in the expression of SCD1 plays an important role in FA and TAG synthesis and can directly or indirectly affect biosynthesis and metabolic pathways in BMECs and may be used as a symbol for important mechanism that contributes the characterization of buffalo milk.

scholarly journals Fatty acid biosynthesis and transcriptional regulation of Stearoyl-CoA Desaturase 1 (SCD1) in Buffalo milk

2019 ◽  
Author(s):  
Zhipeng Li ◽  
Suyu Lu ◽  
Kuiqing Cui ◽  
Laiba Shafique ◽  
Saif ur Rehman ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Epithelial Cells ◽  
Nutritive Value ◽  
Fatty Acid Biosynthesis ◽  
Mammary Epithelial Cells ◽  
Buffalo Milk ◽  
Mammary Epithelial ◽  
Food Component ◽  
Expression Of Genes ◽  
Stearoyl Coa Desaturase

Abstract Background: Nutritionally, buffalo milk is of immense importance due to high contents of fatty acid (FA), highly energetic and nutritive value. The composition of FA has been regarded as functional food component, which facilitate to manufacture a variety of processed milk products. Moreover, Buffalo milk contains more unsaturated FAs (UFA) such as oleic and linolenic acids, which have been shown to exert a number of physiological effects. However, inadequate information reported about the chemical composition and mechanism of FA synthesis in buffalo milk. Our conducted research hypothesize that, the expression of SCD1 gene could alter the biosynthesis of fatty acid (FA) in epithelial cells of mammary gland and subsequently affect the FA contents in buffalo milk. Present study investigated transcriptional and biological role of Stearoyl-CoA Desaturase 1 (SCD1) in the buffalo mammary epithelial cells (BMECs) during FA and triacylglycerol (TAG) synthesis. Results: Results showed that unsaturated fatty acid contents are much higher in concentration in buffalo milk as compared to Holstein cow. Significantly, higher expression level of FAS, ACACA, SREBP1, PPARG, GPAT and AGPAT genes were also been observed with the expression of the SCD1 in buffalo milk. Moreover, studies showed that, SCD1 gene alteration in BMECs also causes the change in the expression of FA synthesis related genes mentioned above and subsequently affect the FA composition in the cells. Overexpression of SCD1 significantly increased the expression of genes associated with FA and TAG synthesis and also improved the FA and unsaturated FA contents in BMECs, while down-regulation of SCD1 appear opposite consequences. Conclusion: Thus, present study clearly demonstrated that, alteration in the expression of SCD1 plays an important role in FA and TAG synthesis and can directly or indirectly affect biosynthesis and metabolic pathways in BMECs and may be used as a symbol for important mechanism that contributes the characterization of buffalo milk.

scholarly journals Fatty acid biosynthesis and transcriptional regulation of Stearoyl-CoA Desaturase 1 (SCD1) in buffalo milk

BMC Genetics ◽  
2020 ◽  
Vol 21 (1) ◽  
Author(s):  
Zhipeng Li ◽  
Suyu Lu ◽  
Kuiqing Cui ◽  
Laiba Shafique ◽  
Saif ur Rehman ◽  
...  
Keyword(s):  
Transcriptional Regulation ◽  
Fatty Acid ◽  
Fatty Acid Biosynthesis ◽  
Buffalo Milk ◽  
Acid Biosynthesis ◽  
Stearoyl Coa Desaturase

Liver X receptor α participates in LPS-induced reduction of triglyceride synthesis in bovine mammary epithelial cells

2020 ◽  
pp. 1-7
Author(s):  
Jianfa Wang ◽  
Shuai Lian ◽  
Jun Song ◽  
Hai Wang ◽  
Xu Zhang ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Epithelial Cells ◽  
Dairy Cow ◽  
Milk Fat ◽  
Mammary Epithelial Cells ◽  
Liver X Receptor ◽  
Mammary Epithelial ◽  
Triglyceride Synthesis ◽  
Milk Fat Depression ◽  
Liver X Receptor Α

Abstract Lipopolysaccharides (LPS) could induce milk fat depression via regulating the body and blood fat metabolism. However, it is not completely clear how LPS might regulate triglyceride synthesis in dairy cow mammary epithelial cells (DCMECs). DCMECs were isolated and purified from dairy cow mammary tissue and treated with LPS. The level of triglyceride synthesis, the expression and activity of the liver X receptor α (LXRα), enzymes related to de novo fatty acid synthesis, and the expression of the fatty acid transporters were investigated. We found that LPS decreased the level of triglyceride synthesis via a down-regulation of the transcription, translation, and nuclear translocation level of the LXRα. The results also indicated that the transcription level of the LXRα target genes, sterol regulatory element binding protein 1 (SREBP1), fatty acid synthetase (FAS), acetyl-CoA carboxylase-1 (ACC1), were significantly down-regulated in DCMECs after LPS treatment. Our data may provide new insight into the mechanisms of milk fat depression caused by LPS.

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