Measurement of Trans-Epithelial Electrical Resistance with EVOM2 and Chopstick Electrode

2020 ◽  
Author(s):  
Shaun D. McCullough
Keyword(s):  
Environmental Protection ◽  
Environmental Protection Agency ◽  
Electrical Resistance ◽  
Cell Monolayer ◽  
Protection Agency ◽  
Commercial Products ◽  
The Us ◽  
Trade Names ◽  
Agency Policy

Abstract Trans-epithelial Electrical Resistance (TEER) can be used as a measure of cell monolayer confluence, health, and integrity. An Epithelial Voltohmmeter (EVOM) is used to take TEER measurements. This method details how to take TEER readings using an EVOM with chopstick electrode.Please note that chopstick electrodes are only designed for use with 12 mm inserts.Disclaimer: The contents of this article have been reviewed by the US Environmental Protection Agency and approved for publication and do not necessarily represent Agency policy. Mention of trade names or commercial products does not constitute endorsement or recommendations for use.

scholarly journals Measurement of Trans-Epithelial Electrical Resistance with EVOM2 and EndOhm Cup

2021 ◽  
Author(s):  
Nicholas M. Mallek ◽  
Shaun D. McCullough
Keyword(s):  
Environmental Protection ◽  
Environmental Protection Agency ◽  
Electrical Resistance ◽  
Cell Monolayer ◽  
Protection Agency ◽  
Commercial Products ◽  
The Us ◽  
Trade Names ◽  
Agency Policy

Abstract Trans-epithelial Electrical Resistance (TEER) can be used as a measure of cell monolayer confluence, health, and integrity. An EndOhm chamber connected to an Epithelial Voltohmmeter (EVOM) may be used to take TEER measurements that are more reproducible than the chopsticks electrode. This method details how to take TEER readings using an EndOhm-12 chamber and EVOM2 resistance reader. Read the manufacturer’s manual carefully before adjusting any of the parameters below as the conditions described here were determined to meet the manufacturer’s specifications. Volumes will need to be adjusted to accommodate substitution of the EndOhm-6 or EndOhm-24.Disclaimer: The contents of this article have been reviewed by the US Environmental Protection Agency and approved for publication and do not necessarily represent Agency policy. Mention of trade names or commercial products does not constitute endorsement or recommendations for use.

scholarly journals Culture of Primary Human Tracheobronchial Epithelial Cells

2021 ◽  
Author(s):  
Lisa Dailey ◽  
Shaun D. McCullough
Keyword(s):  
Epithelial Cells ◽  
Environmental Protection ◽  
Environmental Protection Agency ◽  
Protection Agency ◽  
Commercial Products ◽  
Brush Biopsy ◽  
The Us ◽  
Trade Names ◽  
Agency Policy

Abstract This protocol is intended for culture of primary human tracheobronchial epithelial cells (pHBEC) obtained by brush biopsy during clinical bronchoscopy, or purchased commercially, using Lonza-based medium.Note: This is a historical protocol. At the time of publication, this protocol has been superseded by a different version in the McCullough lab; however, it is being published to support the transparency and reproducibility of other studies by which it is referenced.Disclaimer: The information presented here has been reviewed and approved for publication by the US Environmental Protection Agency do not necessarily represent Agency policy. Mention of trade names or commercial products does not constitute endorsement or recommendations for use.

Production and Concentration of Recombinant Lentiviral Particles

2020 ◽  
Author(s):  
Shaun D. McCullough
Keyword(s):  
Environmental Protection ◽  
Environmental Protection Agency ◽  
Protection Agency ◽  
Commercial Products ◽  
The Us ◽  
Trade Names ◽  
Formal Version ◽  
Agency Policy

Abstract This protocol describes the production of recombinant lentivirus by transfection of HEK293T cells with lentiviral packaging vectors and subsequent concentration by ultracentrifugation. The attached methods document is a formal version of the information included here.Disclaimer: The contents of this article have been reviewed by the US Environmental Protection Agency and approved for publication and do not necessarily represent Agency policy. Mention of trade names or commercial products does not constitute endorsement or recommendations for use.

scholarly journals Transduction of Cultured Cells with Recombinant Lentiviral Particles

2020 ◽  
Author(s):  
Samantha C. Faber ◽  
Eva C.M. Vitucci ◽  
Shaun D. McCullough
Keyword(s):  
Environmental Protection ◽  
Environmental Protection Agency ◽  
Cultured Cells ◽  
Protection Agency ◽  
Commercial Products ◽  
Fluorescent Markers ◽  
The Us ◽  
Trade Names ◽  
Formal Version ◽  
Agency Policy

Abstract This protocol describes the transduction of cultured cells with recombinant lentivirus expressing fluorescent markers. The attached methods document is a formal version of the information included here.Disclaimer: The contents of this article have been reviewed by the US Environmental Protection Agency and approved for publication and do not necessarily represent Agency policy. Mention of trade names or commercial products does not constitute endorsement or recommendations for use.

scholarly journals Culture and Differentiation of Primary Human Tracheobronchial Epithelial Cells Using STEMCELL Technologies Pneumacult Media

2021 ◽  
Author(s):  
Lisa A. Dailey ◽  
Shaun D. McCullough
Keyword(s):  
Epithelial Cells ◽  
Environmental Protection ◽  
Environmental Protection Agency ◽  
Protection Agency ◽  
Commercial Products ◽  
Brush Biopsy ◽  
Previous Version ◽  
The Us ◽  
Scope Of Application ◽  
Trade Names

Abstract NOTE: A PDF methods document is attached in the supplementary materials.SCOPE OF APPLICATION (LIMITATIONS)This method describes the culture and differentiation of primary human tracheobronchial epithelial cells (pHBEC). Cells used for this method can be obtained by brush biopsy during clinical bronchoscopy or purchased commercially. This method replaces the previous version described in (Dailey and McCullough, 2021a; b). Disclaimer: The contents of this article have been reviewed by the US Environmental Protection Agency and approved for publication and do not necessarily represent Agency policy. Mention of trade names or commercial products does not constitute endorsement or recommendations for use.

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