scholarly journals An Ancient Enzyme Finds a New Home: Prevalence and Neofunctionalization of Trypsin in Marine Phytoplankton

2021 ◽  
Author(s):  
Yanchun You ◽  
Xueqiong Sun ◽  
Senjie Lin
Keyword(s):  
Environmental Conditions ◽  
Aquatic Ecosystem ◽  
Digestive Enzyme ◽  
Research Direction ◽  
Potential Functions ◽  
Marine Phytoplankton ◽  
Global Ocean ◽  
Coastal Oceans ◽  
Genome Wide ◽  
New Research

Abstract Trypsin is an ancient protease best known as a digestive enzyme in animals, and traditionally believed to be absent in plants and protists. Here, we surveyed the distribution, diversity, evolution and potential functions of trypsin genes in global ocean phytoplankton, the major primary producers in the aquatic ecosystem. Our analysis indicates that trypsin genes are widely distributed both taxonomically and geographically in marine phytoplankton. Furthermore, by systematic comparative analyses we documented lineage-specific diversity and expansion of trypsin genes in the evolution of marine phytoplankton. Genome-wide analyses revealed that trypsin genes were more prevalent in diatoms than in other lineages. Moreover, the expression of trypsin genes in diatom tended to be more responsive to environmental stimuli. The duplication and neofunctionalization of trypsin genes may be important in diatoms to adapt to dynamical environmental conditions, contributing to diatoms’ dominance in the coastal oceans. This work advances our knowledge on the distributions and neofunctionalizations of this ancient enzyme and creates a new research direction in the phytoplankton biology.

A Robust Blind Image Forensic-Detection Method based on Fuzzy Technique

2019 ◽  
Vol 8 (3) ◽  
pp. 5926-5929
Keyword(s):  
Research Direction ◽  
Geometric Transformation ◽  
Detection Accuracy ◽  
Forensic Investigation ◽  
Image Forensic ◽  
Fuzzy Logic Approach ◽  
Logic Approach ◽  
Tampered Image ◽  
New Research ◽  
Blind Image

Blind forensic-investigation in a digital image is a new research direction in image security. It aims to discover the altered image content without any embedded security scheme. Block and key point based methods are the two dispensation options in blind image forensic investigation. Both the techniques exhibit the best performance to reveal the tampered image. The success of these methods is limited due to computational complexity and detection accuracy against various image distortions and geometric transformation operations. This article introduces different blind image tampering methods and introduces a robust image forensic investigation method to determine the copy-move tampered image by means of fuzzy logic approach. Empirical outcomes facilitate that the projected scheme effectively classifies copy-move type of forensic images as well as blurred tampered image. Overall detection accuracy of this method is high over the existing methods.

scholarly journals Genome-wide identification of the CCCH gene family in rose (Rosa chinensis Jacq.) reveals its potential functions

2021 ◽  
Vol 35 (1) ◽  
pp. 517-526
Author(s):  
Cai-hua Li ◽  
Qing-xi Fang ◽  
Wen-Jing Zhang ◽  
Yu-huan Li ◽  
Jin-zhu Zhang ◽  
...  
Keyword(s):  
Gene Family ◽  
Potential Functions ◽  
Rosa Chinensis ◽  
Genome Wide

scholarly journals Advances in the role of miRNAs in the occurrence and development of osteosarcoma

Open Medicine ◽  
2020 ◽  
Vol 15 (1) ◽  
pp. 1003-1011
Author(s):  
Guanyu Zhang ◽  
Yiran Li ◽  
Jiasheng Xu ◽  
Zhenfang Xiong
Keyword(s):  
Target Gene ◽  
Early Stage ◽  
Research Direction ◽  
Research Progress ◽  
Skeletal System ◽  
Translation Process ◽  
Non Coding Rnas ◽  
New Research ◽  
Post Transcriptional Regulation

AbstractOsteosarcoma (OS) is the most common primary malignant tumor of the skeletal system in the clinic. It mainly occurs in adolescent patients and the pathogenesis of the disease is very complicated. The distant metastasis may occur in the early stage, and the prognosis is poor. MicroRNAs (miRNAs) are non-coding RNAs of about 18–25 nt in length that are involved in post-transcriptional regulation of genes. miRNAs can regulate target gene expression by promoting the degradation of target mRNAs or inhibiting the translation process, thereby the proliferation of OS cells can be inhibited and the apoptosis can be promoted; in this way, miRNAs can affect the metabolism of OS cells and can also participate in the occurrence, invasion, metastasis, and recurrence of OS. Some miRNAs have already been found to be closely related to the prognosis of patients with OS. Unlike other reviews, this review summarizes the miRNA molecules closely related to the development, diagnosis, prognosis, and treatment of OS in recent years. The expression and influence of miRNA molecule on OS were discussed in detail, and the related research progress was summarized to provide a new research direction for early diagnosis and treatment of OS.

Lab Scale Study on Synergistic Disinfection of WWTPS Secondary Effluent by Ultrasonic and Ozone

2012 ◽  
Vol 433-440 ◽  
pp. 4751-4756 ◽  
Author(s):  
Hao Wu ◽  
Zi Fu Li ◽  
Xin Jin ◽  
Xin Zhao ◽  
Fu Rong Deng
Keyword(s):  
Reaction Time ◽  
Removal Rate ◽  
Research Direction ◽  
Synergistic Action ◽  
Color Removal ◽  
Secondary Effluent ◽  
Disinfection Methods ◽  
Number Of Microorganisms ◽  
New Research ◽  
Ozone Disinfection

Secondary effluent from WWTPS still contains a large number of microorganisms, therefore, disinfection is essential. There are many disadvantages in using traditional disinfection methods, so the combination of disinfection techniques is a new research direction. Ozone combined with ultrasound is one of them. In this experiment, the inactivation of the total coli forms, color removal and UV254 removal of secondary effluent by a combination of ultrasonic (frequency20 kHz; power100W) and ozone disinfection was investigated. The results show that the effect of the synergistic action of 30s US and ozone disinfection is superior to the effect of individual ozone disinfection. After 15min synergistic disinfection, the total coli forms inactivation rate is up to 99.9%, simultaneously color removal rate to 80% and UV254 removal rate to 52%. Compared with individual ozone disinfection, reaction time was reduced by 5 min.

A Data Mining Approach to Forming Generic Bills of Materials in Support of Variant Design Activities

2004 ◽  
Vol 4 (4) ◽  
pp. 316-328 ◽  
Author(s):  
Carol J. Romanowski , ◽  
Rakesh Nagi
Keyword(s):  
Data Mining ◽  
Product Family ◽  
Research Direction ◽  
Design Constraints ◽  
Variant Design ◽  
Data Mining Approach ◽  
Design Activities ◽  
Using Data ◽  
New Research

In variant design, the proliferation of bills of materials makes it difficult for designers to find previous designs that would aid in completing a new design task. This research presents a novel, data mining approach to forming generic bills of materials (GBOMs), entities that represent the different variants in a product family and facilitate the search for similar designs and configuration of new variants. The technical difficulties include: (i) developing families or categories for products, assemblies, and component parts; (ii) generalizing purchased parts and quantifying their similarity; (iii) performing tree union; and (iv) establishing design constraints. These challenges are met through data mining methods such as text and tree mining, a new tree union procedure, and embodying the GBOM and design constraints in constrained XML. The paper concludes with a case study, using data from a manufacturer of nurse call devices, and identifies a new research direction for data mining motivated by the domains of engineering design and information.

Vietnamese Proverbs From a Cultural Perspective

2019 ◽  
Author(s):  
Thanh Quy Ngo Thi ◽  
◽  
Hong Minh Nguyen Thi ◽  
Keyword(s):  
Cultural Values ◽  
Language Use ◽  
Interdisciplinary Approach ◽  
Research Direction ◽  
Point Of View ◽  
Traditional Values ◽  
Cultural Perspective ◽  
Language And Culture ◽  
Speakers Of Other Languages ◽  
New Research

Proverbs are important data depicting the traditional culture of each nation. Vietnamese proverbs, dated thousands of years ago, are an immense valuable treasure of experience which the Vietnamese people desire to pass to the younger generations. This paper aims to explore the unique and diversified world of intelligence and spirits of the Vietnamese through a condensed and special literary genre, as well as a traditional value of the nation (Nguyen Xuan Kinh 2013, Tran Ngoc Them 1996, Le Chi Que and Ngo Thi Thanh Quy 2014). Through an interdisciplinary approach, from an anthropological point of view, approaching proverbs we will open up a vast treasure of knowledge and culture of all Vietnamese generations. The study has examined over 16,000 Vietnamese proverbs and analysed three groups expressing Vietnamese people’s behaviors toward nature, society and their selves, and compared them with English and Japanese proverbs. The research has attempted to explore the beauty of Vietnamese language, cultural values and the souls and personalities of Vietnam. Approaching Vietnamese proverbs under the interdisciplinary perspective of language, culture and literature is a new research direction in the field of Social Sciences and Humanity in Vietnam. From these viewpoints, it is seen that proverbs have remarkably contributed to the language and culture of Vietnam as well as and constructed to the practice of language use in everyday life which is imaginary, meaningful and effective in communication. Furthermore, the study seeks to inspire the Vietnamese youth’s pride in national identity and to encourage their preservation and promotion for traditional values of the nation in the context of integration and globalisation. In the meantime, it would be favourable to introduce and market the beauty of Vietnamese language, culture and people to the world, encouraging the speakers of other languages to study, explore and understand Vietnam.

scholarly journals Has-miR-875-5p Inhibits Tumorigenesis by Targeting USF2 via TGF-β Signaling Pathway in Gastric Cancer

2021 ◽  
Author(s):  
Shenshuo Gao ◽  
Zhikai Zhang ◽  
Xubin Wang ◽  
Yan Ma ◽  
Chensheng Li ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Signaling Pathway ◽  
Research Direction ◽  
Luciferase Reporter ◽  
Migration And Invasion ◽  
Gastric Cancer Cells ◽  
New Research

Abstract Background: Gastric cancer (GC) is one of the most common malignancies, and more and more evdiences show that the pathogenesis is regulated by various miRNAs.In this study, we investigated the role of miR-875 in GC. Methods:The expression of miR-875-5p was detected in human GC specimens and cell lines by miRNA RT-PCR. The effect of miR-875-5p on GC proliferation was determined by CCK-8 proliferation assay and EDU assay. Migration and invasion were examined by transwell migration and invasion assay and wound healing assay. The interaction between miR-875-5p and its target gene USF2 was verified by a dual luciferase reporter assay. The effects of miR-875-5p in vivo were studied in xenograft nude mice models.Related proteins were detected by Western blot.Results:The results showed that miR-875-5p inhibited the proliferation, migration and invasion of gastric cancer cells in vitro, and inhibited tumorigenesis in vivo. USF2 proved to be a direct target of miR-875-5p. Knockdown of USF2 partially counteracts the effects of miR-875-5p inhibitors.Overexpression of miR-875-5p can inhibit proliferation, migration, and invasion through the TGF-β signaling pathway by down-regulation of USF2 in GC, providing a new research direction for the diagnosis and targeted therapy of GC.Conclusions: MiR-875-5pcan inhibited the progression of GC by directly targeting USF2 and negatively regulating TGF-β signaling pathway.In the future, miR-875-5p is expected to be used as a potential therapeutic target for GC therapy.

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