scholarly journals Efficient Plant Regeneration System for New Guinea Impatiens (Impatiens Hawkeri W. Bull) CV. ‘Violet’and ‘Scarlet Bronze Leaf’

2021 ◽  
Author(s):  
Di Zhang ◽  
Jie Wei ◽  
Min Zhou ◽  
Yang Li ◽  
Xin Yi Li ◽  
...  
Keyword(s):  
Plant Regeneration ◽  
Growth Regulators ◽  
New Guinea ◽  
Basic Medium ◽  
Regeneration System ◽  
Regeneration Rate ◽  
Induction Rate ◽  
Impatiens Hawkeri ◽  
New Guinea Impatiens

Abstract New Guinea Impatiens (Impatiens hawkeri W. Bull) is an eye-popping landscaping plant which is of bright and colorful blooms. A highly efficient in vitro plant regeneration system through direct shoot organogenesis was established for the first time from hypocotyl with partial cotyledons of New Guinea Impatiens. The results showed that Explant sterilization method, basic medium type,AgNO3,sucrose and plant growth regulators (PGRs) have greatly influences on in vitro morphogenesis.The regeneration rate in regeneration media that MS supplemented with 0.5mg·L−1 TDZ and 0.1mg·L−1 NAA was acceptable ,the induction rate of 'Violet' was 86.67%, and its proliferation coefficient was 5.27, while the induction rate of 'Scarlet Bronze Leaf' was 83.33%, and its proliferation coefficient was 5.13.PIC was unable to induce clumped sprouts, but it had a better effect on callus induction.We also included a shoot multiplication stage using regeneration New Guinea Impatiens medium that MS supplemented with 0.8mg·L−1 6-BA,0.5mg·L−1 TDZ and 0.05mg ·L−1 NAA.Reducing sucrose concentration to 20g·L−1 or adding 1mg·L−1AgNO3 could alleviate the vitrification phenomenon in the process of tufted bud proliferation.The optimal root culture medium for the regenerated seedlings of 'violet' and 'scarlet bronze leaf' of New Guinea Impatiens was MS supplemented with 0.05mg·L−1IBA, the rooting rate reached 100%.The study examined the micropropagation responses of New Guinea Impatiens in the presence of various growth regulators and provided a simple and more suitable protocol adapted for the mass propagation of clones.

scholarly journals Direct and Efficient Plant Regeneration from Different Explants Sources of Potato Cultivars as Influenced by Plant Growth Regulators

2012 ◽  
Vol 12 ◽  
pp. 1-6 ◽  
Author(s):  
Shambhu P. Dhital ◽  
Hak T. Lim ◽  
Hira K. Manandhar
Keyword(s):  
Plant Regeneration ◽  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Solid Medium ◽  
Naphthaleneacetic Acid ◽  
Regeneration System ◽  
Petiole Explants ◽  
Number Of Shoots

Response of widely grown potato cv. Superior and newly developed cvs. Gui valley and Bora valley to plant growth regulators (PGRs) for direct plant regeneration from internode, leaf blade and petiole explants were investigated. The explants were cultured on a MS solid medium supplemented with different concentrations and combinations of 6-benzylaminopurine (BAP), 1-naphthaleneacetic acid (NAA), zeatin, indole-3-acetic acid (IAA) and gibberellic acid (GA3). Potato cv. Superior, regenerated direct shoot without callus and root formation on MS solid medium supplemented with BAP or zeatin, proliferous roots were produced on NAA or IAA supplemented medium and only some calli were produced on GA3 supplemented medium. The regeneration response varied with different concentrations of PGRs, singly and also in combinations. In the case of combined application of PGRs, the highest shoot regeneration (75.3%) and number of shoot per explant (11.5) and number of roots per explant (7.0) were obtained from the MS solid medium supplemented with zeatin (2 mg l-1), NAA (0.1 mg l-1) and GA3 0.1 mg l-1). Among the three types of explants evaluated, internodes produced the highest number of shoots and roots for both potato cvs. Gui valley and Bora valley, and petiole produced the least number of shoots and roots. The regenerated shoots were rooted in PGRs-free MS solid medium and successfully established under glasshouse condition. Leaf, flower, and tuber morphology were identical to in vitro control and mother plants in the same conditions. This optimized regeneration system can be used for rapid shoot proliferation and also for gene transformation.DOI: http://dx.doi.org/10.3126/njst.v12i0.6471 Nepal Journal of Science and Technology 12 (2011) 1-6 

scholarly journals Plant Regeneration of Periwinkle (Catharanthus roseus) via Organogenesis

HortScience ◽  
2008 ◽  
Vol 43 (3) ◽  
pp. 832-836 ◽  
Author(s):  
Andrea Swanberg ◽  
Wenhao Dai
Keyword(s):  
Plant Regeneration ◽  
Shoot Regeneration ◽  
Woody Plant ◽  
Naphthalene Acetic Acid ◽  
Regeneration System ◽  
Dark Treatment ◽  
Regeneration Rate ◽  
Shoot Production ◽  
Leaf Tissues

Two periwinkle cultivars, Pacific Coral (P1) and Sunstorm Rose (P2), were used for development of a plant regeneration system. Leaf and internodal explants collected from in vitro plants were plated onto woody plant medium (WPM) using a factorial arrangement of 6-benzyladeine (BA) and 1-naphthalene acetic acid (NAA). Shoots were successfully regenerated. Shoot production from leaf tissues was minimal for all cultivars, whereas internodal tissues showed variable rates of regeneration depending on the hormone combination. Cultivar P1 showed the maximum regeneration rate (73.3%) when internodal explants, 4 to 6 mm in length, were placed on WPM containing 5 μm BA and 5 μm NAA. Cultivar P2 showed a regeneration rate of 56.7% with a combination of 20 μm BA and 10 μm NAA. Shoot regeneration rate increased as the internodal explant size increased for P2; however, the regeneration rate decreased when the explant size was greater than 7 mm for P1. The shoot regeneration rate decreased as the period of the dark treatment of internodal explants increased in both P1 and P2. The antibiotics carbenicillin (Carb) and cefotaxime (Cef) had little effect on shoot regeneration. There was a slightly higher rate observed for P1 when Cef was added into the medium, whereas P2 showed a decrease with the addition of Cef. Carb showed no significant effect on shoot regeneration for both cultivars. Addition of both Carb and Cef to the medium slightly inhibited shoot regeneration.

scholarly journals In vitro organogenesis and plant regeneration of Passiflora xishuangbannaensis, a species with extremely small populations

2021 ◽  
Author(s):  
Yuan-yuan Meng ◽  
Shi-jie Song ◽  
Sven Landrein
Keyword(s):  
Plant Regeneration ◽  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Basal Medium ◽  
Ex Situ ◽  
Naphthaleneacetic Acid ◽  
South American ◽  
South American Species

Abstract Passiflora xishuangbannaensis (Passifloraceae) is endemic to a few sites of Mengyang nature reserve in Yunnan, Xishuangbanna and less than 40 individuals have been recorded. Nine Passiflora species are endemic to Yunnan with most species occurring in South America, making P. xishuangbannaensis highly significant and emblematic to the conservation work in the region. This study is designed to provide the first protocol for in vitro organogenesis and plant regeneration for ex situ conservation and reintroduction for an Asian Passiflora species. Using internodes, petioles and tendrils we optimize calli formation and root elongation using several plant growth regulators, individually or in combination. We also assess the genetic stability of regenerated cells. The maximum callus induction and shoot bud differentiation were both achieved on half Murashige and Skoog basal medium supplemented with 4.44 µM 6-Benzylaminopurine and 1.08 µM 1-Naphthaleneacetic acid. The best rooting was achieved from 30 days old, regenerated shoots on half Murashige and Skoog basal medium supplemented with 1.08 µM 1-Naphthaleneacetic acid. Micropropagated plants were subjected to inter simple sequence repeat markers analyses. Collectively, 86 bands were generated from 6 primers of which 12 bands were polymorphic, showing genetic variation between the regenerated plantlets and the original plant. Response to plant growth regulators was more specific than most other studies using South American species, which could be explained by the morphological and physiological differences between South American and Asian Passiflora species

scholarly journals Introduction to in vitro culture and callus initiation in Salvia hispanica L. (chia)

2019 ◽  
Vol 17 (1) ◽  
pp. 33-37
Author(s):  
A. Z. Revutskaya ◽  
A. V. Holubenko ◽  
N. V. Nuzhyna ◽  
H. O. Rudik ◽  
N. Yu. Taran
Keyword(s):  
In Vitro Culture ◽  
Growth Regulators ◽  
Nutrient Medium ◽  
Callus Growth ◽  
Basic Medium ◽  
High Concentration ◽  
Salvia Hispanica ◽  
Callus Initiation ◽  
Salvia Hispanica L

Aim. Preparation of aseptic seedlings Salvia hispanica L., callus initiation in vitro and establishment of primary explants suitable for the callus production. Methods. Seeds are sprouted on our own modification of conventional methods. The non-hormonal Murashige-Skoog agarized nutrient medium was used as basic medium for the experiments. Parts of one-month seedlings (roots, hypocotyl, cotyledon leaves) were used as explants for the use of the colza. We added growth regulators (BAP, 2,4-D) in different concentration combinations into the nutrient medium for callus initiation. Statistical processing was performed in Microsoft Office Excel. Results. Aseptic S. hispanica seedlings have been obtained. The callus growth was initiated on all types of explants, the dependence of the callus intensity on the type of explants and the growth regulators content in the nutrient medium was established. Morphogenic callus and root-regenerants have been obtained. Conclusions. Hypocotyl was the most suitable primary explant for callus growth. Seedlings, leaves and roots showed low morphogenetic capacity. The nutrient medium with an elevated 2,4-D content was the most effective for initiation of callus genesis and proliferation of non-morphogenous callus. A high concentration of 2,4-D in the medium improves S. hispanica callus growth but suppresses its morphogenic ability.Keywords: Salvia hispanica (Chia), in vitro culture, callus.

In vitro plant regeneration from leaf expiants of Lycopersicon esculentum (tomato)

1976 ◽  
Vol 54 (21) ◽  
pp. 2409-2414 ◽  
Author(s):  
R. M. Behki ◽  
S. M. Lesley
Keyword(s):  
Plant Regeneration ◽  
Lycopersicon Esculentum ◽  
Growth Regulators ◽  
In Vitro Plant Regeneration ◽  
Leaf Discs ◽  
Skoog Medium ◽  
Murashige And Skoog Medium ◽  
Napthaleneacetic Acid ◽  
In Vitro Plant

Leaf discs from 15 mutant clones of tomato were tested for their morphogenetic response in Murashige and Skoog medium supplemented with 12 combinations of the growth regulators napthaleneacetic acid (NAA) and benzylaminopurine (BA) and 4 combinations of NAA and zeatin. The results show that either callus, shoots, roots, or shoots and roots can be produced depending upon the hormone concentrations and ratios. Plants were regenerated from 12 of the 15 varieties tested.

scholarly journals Haploid plantlet regeneration through anther culture in oilseed Brassica species

1970 ◽  
Vol 34 (4) ◽  
pp. 693-703 ◽  
Author(s):  
MA Alam ◽  
MA Haque ◽  
MR Hossain ◽  
SC Sarker ◽  
R Afroz
Keyword(s):  
Plant Regeneration ◽  
Anther Culture ◽  
Growth Regulators ◽  
Callus Induction ◽  
Brassica Species ◽  
Plantlet Regeneration ◽  
Root Induction ◽  
Ms Medium ◽  
Half Strength

Anther of five varieties of Brassica species, namely BARI Shariaha-7, Tori-7, Agrani, Daulat and Safal were cultured in vitro to observe their regeneration potentiality. Different concentrations and combinations of growth regulators were supplemented in MS medium. The range of callus induction was 12.50-87.50 %. Maximum callus induction (75.00%) was observed on MS +4 mg/L 2, 4-D + 1.0 mg/L BAP. Among the genotypes, BARI Sharisha-7 showed the highest percentage of callus induction (60.42%). Among the treatments, highest percentage of shoot regeneration (75.00%) was observed on MS + 4 mg/L BAP + 1.0 mg/L NAA. BARI Sharisha-7 also showed the highest rate of plant regeneration (66.67%). Root induction was highest (75%) on half strength MS medium supplemented with 1.0 mg/L IBA and 0.5 mg/L NAA. The plantlets with sufficient roots thus obtained were transferred successfully to plastic pots and subsequently to the field. BARI Sharisha-7 and Tori-7 survived easily in the pots as well as in the field but Safal was very poor in survivability both in the pots and in the field. Key Words: Brassica; haploid; anther culture; in vitro regeneration.DOI: 10.3329/bjar.v34i4.5844Bangladesh J. Agril. Res. 34(4) : 693-703, December 2009 

REGENERATION OF TREMA ORIENTALIS (BLUME) LINN.: EFFECT OF GROWTH REGULATORS, CULTURE CONDITIONS, AND AGE AND SOURCE OF EXPLANTS

1995 ◽  
Vol 43 (4) ◽  
pp. 391-395 ◽  
Author(s):  
G.R. Rout ◽  
S. Samantaray ◽  
P. Das
Keyword(s):  
Growth Regulators ◽  
Leaf Explants ◽  
Naphthaleneacetic Acid ◽  
Mature Trees ◽  
Regeneration Rate ◽  
Bud Regeneration ◽  
Shoot Bud ◽  
Shoot Bud Regeneration ◽  
Trema Orientalis

Optimal conditions for high frequency shoot bud regeneration from leaf callus of Trema orientalis (Blume) Linn. were studied. The regeneration rate was controlled by the growth regulators, the age and the source of the explants, and the illumination conditions. Irrespective of illumination conditions, shoot bud regeneration was achieved only in media containing benzyladenine (BA) + α-naphthaleneacetic acid (NAA) combinations, with the best results being obtained in the presence of 2.5 mg/1 BA and 0.25–0.5 mg/1 NAA. The morphogenic response was less frequent in the calluses derived from leaf explants of the mature trees compared to those of the in vitro-grown seedlings. The rate of shoot bud regeneration was more pronounced in the cultures maintained for 4 weeks in the light (16-h photoperiod) than the cultures incubated in the dark. Regenerated shoots were rooted on the medium containing 1/2 strength basal Murashige and Skoog (MS) salts supplemented with 0.01 mg/1 NAA or indole-3-butyric acid (IBA). The rooted plantlets were established in the greenhouse.

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