scholarly journals Protective Role of Nrf2 in Zinc Oxide Nanoparticles-Induced Lung Inflammation in Female Mice and Sexual Dimorphism in Susceptibility

2022 ◽  
Author(s):  
Radwa Sehsah ◽  
Wenting Wu ◽  
Sahoko Ichihara ◽  
Naozumi Hashimoto ◽  
Cai Zong ◽  
...  
Keyword(s):  
Zinc Oxide ◽  
Proinflammatory Cytokines ◽  
Lung Inflammation ◽  
Zinc Oxide Nanoparticles ◽  
Synergistic Effects ◽  
Oxide Nanoparticles ◽  
Mrna Levels ◽  
Male Mice ◽  
Zno Nps ◽  
Female Mice

Abstract Background Zinc oxide nanoparticles (ZnO-NPs) are used in various products such as rubber, paint, and cosmetics. Our group reported recently that Nrf2 protein provides protection against ZnO-NPs-induced pulmonary inflammation in male mice. The present study investigated the effect of Nrf2 deletion on the lung inflammatory response in female mice exposed to ZnO-NPs. Methods Twenty-four female Nrf2−/− mice and the same number of female Nrf2+/+ mice were each divided into three equal groups and each exposed to ZnO-NPs at either 0, 10 or 30 µg/mouse by pharyngeal aspiration. Bronchoalveolar lavage fluid (BALF) and lungs were collected 14 days later to quantify protein level, number of inflammatory cells, and for scoring inflammation histopathologically. The mRNA levels of Nrf2-depedent antioxidant enzymes and proinflammatory cytokine in lung tissue were measured. Results Exposure to ZnO-NPs increased all types of BALF cells and lung inflammation scores in both of female Nrf2−/− and Nrf2+/+ mice, and Nrf2 deletion enhanced ZnO-NPs-induced increase in the number of eosinophils in BALF. Nrf2 deletion enhanced ZnO-NPs-induced downregulation of GR and upregulation of HO-1 and TNFα. Nrf2 deletion decreased mRNA levels of CAT, GcLc and NQO1 and increased that for GcLm and MT-2. ZnO-NPs dose-dependently increased the level of oxidized glutathione (GSSG), and mRNA levels of proinflammatory cytokines/chemokines; KC, MIP-2, IL-6, IL-1β and MCP-1 only in wild-type mice, and Nrf2 deletion decreased total glutathione levels and upregulated the above proinflammatory cytokines/chemokines regardless of level of exposure to ZnO-NPs. Taken together with our previous results in male mice, our results showed a lower susceptibility of females to lung inflammation, relative to males, irrespective of Nrf2 deletion, and that enhancement of ZnO-NPs-induced upregulation of HO-1 and TNFα and downregulation of GR by deletion of Nrf2 is specific to female mice. Conclusion We conclude that Nrf2 provides protection in female mice against increase in BALF eosinophils, probably through down-regulation of proinflammatory cytokines/chemokines and upregulation of oxidative stress-related genes. The study also suggests lower susceptibility to lung inflammation in female mice relative to their male counterparts and the synergistic effects of sex and exposure to ZnO-NPs on mRNA expression of GR, HO-1 or TNFα.

scholarly journals Evaluation of the Toxicological Effects of Zinc Oxide Nanoparticles in Albino Male Mice

2020 ◽  
pp. 42-58
Author(s):  
Sarab Mohammed M. Razooki ◽  
Adel M. Rabee
Keyword(s):  
Zinc Oxide ◽  
Zinc Oxide Nanoparticles ◽  
Seminiferous Tubules ◽  
Oxide Nanoparticles ◽  
High Dose ◽  
Dependent Manner ◽  
Male Mice ◽  
Zno Nps ◽  
Toxicological Effect ◽  
Histopathological Effects

The acute and sub chronic toxicity effects of 25.16 nm intraperitoneally- injected zinc oxide nanoparticles (ZnO NPs) were evaluated. Albino male mice were exposed to three different doses (25, 50 ,and 100 mg/kg ), depending on the value of calculated LD50, for 2 and 4 weeks . Considerable changes in organ indexes were shown with a good relevance to the illustrated histopathological effects which ranged from multiple hemorrhagic foci in liver, mild swelling and dilatation in kidney tubules, thickening of intestinal villi, moderate interstitial pneumonia, especially with the high dose , and sever necrosis of seminiferous tubules in testes of all studied groups. Significant changes in both hematological and biochemical parameters as well as thyroid hormones were observed with a considerable increase in the levels of antioxidant enzymes, in dose and exposure time dependent manner. The highest accumulated Zn mean values were recorded in the small intestine, kidney, liver, and spleen, respectively, followed by testes , heart , lung , and brain. These values followed the same order of the dose dependent manner, which explains the adverse effects that were recorded. This study proved the ability of using organ indexes as good tools side by side with the biochemical indicators to explain the histopathological changes. This study also revealed some histopathological effects that were not previously recorded as a toxicological effect of ZnO NPs in animal models.   

scholarly journals Zinc oxide nanoparticles improve testicular steroidogenesis machinery dysfunction in benzo[α]pyrene-challenged rats

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Niveen M. Daoud ◽  
Mohamed S. Aly ◽  
Omaima H. Ezzo ◽  
Naglaa A. Ali
Keyword(s):  
Oxidative Stress ◽  
Zinc Oxide ◽  
Zinc Oxide Nanoparticles ◽  
Molecular Mechanisms ◽  
Regulatory Protein ◽  
Male Rats ◽  
Oxide Nanoparticles ◽  
Mrna Levels ◽  
Zno Nps ◽  
Positive Effects

AbstractZinc oxide nanoparticles (ZnO NPs) demonstrate potential positive effects on reproduction. However, their protective role against the reproductive toxicity pollutants has not yet been adequately studied at the molecular level. This study was designed to assess this objective using Benzo[α]pyrene B[a]P as reproductive toxic agent . Forty-eight mature male rats were randomly distributed into six groups: Group1 (negative control); Groups 2 and 3 (positive control I and II, wherein the animals were treated with 10 and 30 mg ZnO NPs/kg BW, respectively); Group 4 (B[a]P group; treated with 150 mg B[a]P/kg BW); and Groups 5 and 6 (subjected to B[a]P treatment co-administered with different concentrations of ZnO NPs). We investigated oxidative stress biomarkers; cholesterol side-chain cleavage enzyme (CYP11A1), steroidogenic acute regulatory protein (StAR), and 3β-hydroxysteroid dehydrogenase (3β-HSD) gene expression; testosterone levels; and histopathology of the liver, kidney, and testicles. The B[a]P-treated group showed significant deterioration in all reproductive parameters and displayed induced oxidative stress. ZnO NPs remarkably reduced oxidative stress, effectively upregulated the mRNA levels of CY11A1, StAR, and 3β-HSD, and improved the histological pictures in the examined organs. At their investigated doses and given their NPs properties, ZnO NPs demonstrated a marked ameliorative effect against the reproductive toxic effects of B[a]P. Further studies are needed to thoroughly investigate the molecular mechanisms of ZnO NPs.

scholarly journals Histopathological Effect of Zinc Oxide Nanoparticles on Kkidney and Liver Tissues in Albino Male Mice

2018 ◽  
Vol 31 (1) ◽  
pp. 9 ◽  
Author(s):  
Ruqayah Ali Salman
Keyword(s):  
Zinc Oxide ◽  
Zinc Oxide Nanoparticles ◽  
Histopathological Examination ◽  
Control Group ◽  
Oxide Nanoparticles ◽  
Male Mice ◽  
Zno Nps ◽  
Liver And Kidney ◽  
Dose Levels ◽  
Liver Tissues

    Objective: The present study investigated the effects of different dose levels of Zinc oxide Nanoparticles (ZnO NPs) on the liver and kidney tissues in albino male mice. Methadology: ZnO NPs was administrated as a daily oral dose of (150, 350 mg/kg body weight) gavage for 2 weeks. Eighteen male mices were used by dividing them into three groups. Result: Histopathological examination of kidney and hepatic tissues treated with ZnO NPs showed toxicity changes compared with control group. Conclusion:This study demonstrated the ability of ZnO NPs to affect on kidney and liver tissues. Recommendation: More study needed to know the effect of different doses of nanoparticles on human health.  

Investigations on synergistic and antioxidant actions of medicinal plant based biosynthesis of Zinc Oxide Nanoparticles against E.coli and K. pneumonia bacteria

2021 ◽  
Vol 24 ◽  
Author(s):  
Farzana Rashid ◽  
Iqra Pervaiz ◽  
Husna Malik ◽  
Zakia Kanwal ◽  
Muhammad Rafique ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Zinc Oxide ◽  
Antibacterial Activity ◽  
Zinc Oxide Nanoparticles ◽  
Bacterial Resistance ◽  
Synergistic Effects ◽  
Oxide Nanoparticles ◽  
Zno Nps ◽  
Dpph Method ◽  
Multiple Drugs

Introduction: Bacterial resistance to multiple drugs is increasing at an alarming rate in current era and nanotechnology is one of the effective and novel approaches to overcome drug resistance. Methods: Zinc Oxide Nanoparticles (ZnO NPs) has stronger antibacterial activity and is regarded as bio-safe nanomaterial. The aim of present study is to synthesize the ZnO NPs using Aloe vera leaves extract and to investigate the synergistic effects and antioxidant actions of bio-synthesized ZnO NPs against gram negative bacteria E.coli and K. pneumoniae. The synergistic effect of β-lactam antibiotics (meropenem and ciprofloxacin) was tested along with ZnO NPs by using Kirby’s disc diffusion assay. The antioxidant activity was investigated by α, α-diphenyl-β-picrylhydrazyl (DPPH) method. Results: Results revealed that the antibacterial activity of the selected antibiotics was much enhanced by ZnO NPs than the antibiotics alone. The resistant antibiotic (ciprofloxacin) became sensitive when combined with ZnO NPs. The antioxidant activity reveals that biosynthesized ZnO NPs possess significantly higher (p<0.05) antioxidant activity (77%). Conclusion: The findings reveal that biosynthesized ZnO NPs have much more eco-friendly approach. It can act as a strong potentiator of β-lactam antibiotics and put forward the possibility to use them effectively in targeted drug delivery, pharmaceuticals and biomedical fields.

Evaluation of the Effect of Nanoparticles Zinc Oxide/Camellia sinensis Complex on the Kidney of Rats Treated with Monosodium Glutamate: Antioxidant and Histological Approaches

2019 ◽  
Vol 20 (7) ◽  
pp. 542-550 ◽  
Author(s):  
Nahla S. El-Shenawy ◽  
Reham Z. Hamza ◽  
Fawziah A. Al-Salmi ◽  
Rasha A. Al-Eisa
Keyword(s):  
Zinc Oxide ◽  
Camellia Sinensis ◽  
Zinc Oxide Nanoparticles ◽  
Morphological Changes ◽  
Monosodium Glutamate ◽  
Oxide Nanoparticles ◽  
High Dose ◽  
Enzymatic Antioxidants ◽  
Zno Nps ◽  
Tea Extract

Background: Zinc oxide nanoparticles (ZnO NPs) are robustly used biomedicine. Moreover, no study has been conducted to explore the consequence of green synthesis of ZnO NPs with Camellia sinensis (green tea extract, GTE) on kidneys of rats treated with monosodium glutamate (MSG). Methods: Therefore, the objective of the research was designed to explore the possible defensive effect of GTE/ZnO NPs against MSG-induced renal stress investigated at redox and histopathological points. Results: The levels of urea and creatinine increased as the effect of a high dose of MSG, in addition, the myeloperoxidase and xanthine oxidase activates were elevated significantly with the high dose of MSG. The levels of non-enzymatic antioxidants (uric acid, glutathione, and thiol) were decreased sharply in MSG-treated rats as compared to the normal group. Conclusion: The data displayed that GTE/ZnO NPs reduced the effects of MSG significantly by reduction of the level peroxidation and enhancement intracellular antioxidant. These biochemical findings were supported by histopathology evaluation, which showed minor morphological changes in the kidneys of rats.

Zinc oxide nanoparticles help to enhance plant growth and alleviate abiotic stress: A review

2020 ◽  
Vol 21 ◽  
Author(s):  
Mohammad Faizan ◽  
Fangyuan Yu ◽  
Chen Chen ◽  
Ahmad Faraz ◽  
Shamsul Hayat
Keyword(s):  
Zinc Oxide ◽  
Plant Growth ◽  
Abiotic Stresses ◽  
Zinc Oxide Nanoparticles ◽  
Growth And Yield ◽  
Oxide Nanoparticles ◽  
Main Concern ◽  
Negative Effects ◽  
Zno Nps ◽  
Agricultural Yield

: Abiotic stresses arising from atmosphere change belie plant growth and yield, leading to food reduction. The cultivation of a large number of crops in the contaminated environment is a main concern of environmentalists in the present time. To get food safety, a highly developed nanotechnology is a useful tool for promoting food production and assuring sustainability. Nanotechnology helps to better production in agriculture by promoting the efficiency of inputs and reducing relevant losses. This review examines the research performed in the past to show how zinc oxide nanoparticles (ZnO-NPs) are influencing the negative effects of abiotic stresses. Application of ZnO-NPs is one of the most effectual options for considerable enhancement of agricultural yield globally under stressful conditions. ZnO-NPs can transform the agricultural and food industry with the help of several innovative tools in reversing oxidative stress symptoms induced by abiotic stresses. In addition, the effect of ZnO-NPs on physiological, biochemical, and antioxidative activities in various plants have also been examined properly. This review summarizes the current understanding and the future possibilities of plant-ZnO-NPs research.

scholarly journals Wound Healing Composite Materials of Bacterial Cellulose and Zinc Oxide Nanoparticles with Immobilized Betulin Diphosphate

Nanomaterials ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 713
Author(s):  
Nina Melnikova ◽  
Alexander Knyazev ◽  
Viktor Nikolskiy ◽  
Peter Peretyagin ◽  
Kseniia Belyaeva ◽  
...  
Keyword(s):  
Wound Healing ◽  
Zinc Oxide ◽  
Bacterial Cellulose ◽  
Zinc Oxide Nanoparticles ◽  
Wound Dressings ◽  
Oxide Nanoparticles ◽  
Burn Wound ◽  
Zno Nps ◽  
Wound Model ◽  
And Oxidative Stress

A design of new nanocomposites of bacterial cellulose (BC) and betulin diphosphate (BDP) pre-impregnated into the surface of zinc oxide nanoparticles (ZnO NPs) for the production of wound dressings is proposed. The sizes of crystalline BC and ZnO NPs (5–25%) corresponded to 5–6 nm and 10–18 nm, respectively (powder X-ray diffractometry (PXRD), Fourier-infrared (FTIR), ultraviolet (UV), atomic absorption (AAS) and photoluminescence (PL) spectroscopies). The biological activity of the wound dressings “BC-ZnO NPs-BDP” was investigated in rats using a burn wound model. Morpho-histological studies have shown that more intensive healing was observed during treatment with hydrophilic nanocomposites than the oleophilic standard (ZnO NPs-BDP oleogel; p < 0.001). Treatment by both hydrophilic and lipophilic agents led to increases in antioxidant enzyme activity (superoxide dismutase (SOD), catalase) in erythrocytes and decreases in the malondialdehyde (MDA) concentration by 7, 10 and 21 days (p < 0.001). The microcirculation index was restored on the 3rd day after burn under treatment with BC-ZnO NPs-BDP wound dressings. The results of effective wound healing with BC-ZnO NPs-BDP nanocomposites can be explained by the synergistic effect of all nanocomposite components, which regulate oxygenation and microcirculation, reducing hypoxia and oxidative stress in a burn wound.

scholarly journals Biosynthesis of Zinc Oxide Nanoparticles from Acacia nilotica (L.) Extract to Overcome Carbapenem-Resistant Klebsiella Pneumoniae

Molecules ◽  
2021 ◽  
Vol 26 (7) ◽  
pp. 1919
Author(s):  
Elsayim Rasha ◽  
AlOthman Monerah ◽  
Alkhulaifi Manal ◽  
Ali Rehab ◽  
Doud Mohammed ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Zinc Oxide ◽  
Klebsiella Pneumoniae ◽  
Zinc Oxide Nanoparticles ◽  
Acacia Nilotica ◽  
Oxide Nanoparticles ◽  
Zno Nps ◽  
X Ray ◽  
Carbapenem Resistant

Recently, concerns have been raised globally about antimicrobial resistance, the prevalence of which has increased significantly. Carbapenem-resistant Klebsiella pneumoniae (KPC) is considered one of the most common resistant bacteria, which has spread to ICUs in Saudi Arabia. This study was established to investigate the antibacterial activity of biosynthesized zinc oxide nanoparticles (ZnO-NPs) against KPC in vitro and in vivo. In this study, we used the aqueous extract of Acacia nilotica (L.) fruits to mediate the synthesis of ZnO-NPs. The nanoparticles produced were characterized by UV-vis spectroscopy, zetasizer and zeta potential analyses, X-ray diffraction (XRD) spectroscopy, Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDX), and transmission electron microscopy (TEM). The antimicrobial activity of ZnO-NPs against KPC was determined via the well diffusion method, and determining minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC), the results showed low MIC and MBC when compared with the MIC and MBC of Imipenem and Meropenem antibiotics. The results of in vitro analysis were supported by the results upon applying ZnO-NP ointment to promote wound closure of rats, which showed better wound healing than the results with imipenem ointment. The biosynthesized ZnO-NPs showed good potential for use against bacteria due to their small size, applicability, and low toxicity to human cells.

scholarly journals Scolicidal activity of biosynthesized zinc oxide nanoparticles by Mentha longifolia L. leaves against Echinococcus granulosus protoscolices

2021 ◽  
Author(s):  
Bushra H. Shnawa ◽  
Samir M. Hamad ◽  
Azeez A. Barzinjy ◽  
Payman A. Kareem ◽  
Mukhtar H. Ahmed
Keyword(s):  
Zinc Oxide ◽  
Zinc Oxide Nanoparticles ◽  
Public Health Problem ◽  
Analytical Techniques ◽  
Oxide Nanoparticles ◽  
Zno Nps ◽  
Leaf Extracts ◽  
X Ray ◽  
Morphological Alterations ◽  
Mentha Longifolia

AbstractCystic echinococcosis is a public health problem in developing countries that practice sheep breeding extensively. In the current study, the protoscolicidal activity of biosynthesized zinc oxide nanoparticles (ZnO NPs) derived from Mentha longifolia L. leaf extracts was investigated. The resultant ZnO NPs were characterized by means of various analytical techniques, such as ultraviolet–visible (UV–Vis) spectrometry, Fourier transform infrared (FT-IR) spectrophotometry, X-ray diffraction (XRD), scanning electron microscopy (SEM), and energy-dispersive X-ray spectroscopy (EDX) analysis. The results showed that the ZnO NP had the highest scolicidal activity at 400 ppm concentration after 150 min of exposure time, showing 100% mortality rate. The treated protoscolices exhibited loss of viability with several morphological alterations. Hence, an easy and effective green synthesis of ZnO NPs, with efficient scolicidal potential, is reported in this study.

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